ES2478872T3 - Composición líquida que comprende una proteasa aspártica - Google Patents

Composición líquida que comprende una proteasa aspártica Download PDF

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Publication number
ES2478872T3
ES2478872T3 ES11156545.3T ES11156545T ES2478872T3 ES 2478872 T3 ES2478872 T3 ES 2478872T3 ES 11156545 T ES11156545 T ES 11156545T ES 2478872 T3 ES2478872 T3 ES 2478872T3
Authority
ES
Spain
Prior art keywords
liquid composition
aspartic protease
count
malate
formate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES11156545.3T
Other languages
English (en)
Inventor
André De Haan
Mylene Caussette
Margot Elisabeth Francoise Schooneveld-Bergmans
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
DSM IP Assets BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=36950288&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2478872(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV
Application granted granted Critical
Publication of ES2478872T3 publication Critical patent/ES2478872T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/032—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
    • A23C19/0326—Rennet produced by fermentation, e.g. microbial rennet; Rennet produced by genetic engineering
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
    • C12N9/6405—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
    • C12N9/6413—Aspartic endopeptidases (3.4.23)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6483—Chymosin (3.4.23.4), i.e. rennin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23004—Chymosin (3.4.23.4), i.e. rennin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Dairy Products (AREA)

Abstract

Composición líquida que comprende: (i) una proteasa aspártica de Rhizomucor miehei y (ii) una sal inorgánica y (iii) un compuesto seleccionado de formiato, acetato, lactato, propionato, malato o fumarato composición en que: la concentración total de sorbato, benzoato y ésteres alquílicos de para-hidroxibenzoato es menor que 0,010 moles/l; el recuento en placa clásico <= 100 en 1 ml; recuento de levaduras <= 10 en 1 ml y recuento de mohos <= 10 en 1 ml y en la que el pH está entre 4,8 y 5,5 y en que la concentración de formiato, acetato, lactato, propionato, malato o fumarato o combinación de los mismos es al menos 0,1 moles/l.

Description

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Claims (1)

  1. imagen1
    imagen2
ES11156545.3T 2006-04-13 2007-04-12 Composición líquida que comprende una proteasa aspártica Active ES2478872T3 (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US79144706P 2006-04-13 2006-04-13
EP06112649 2006-04-13
EP06112649 2006-04-13
US791447P 2006-04-13

Publications (1)

Publication Number Publication Date
ES2478872T3 true ES2478872T3 (es) 2014-07-23

Family

ID=36950288

Family Applications (1)

Application Number Title Priority Date Filing Date
ES11156545.3T Active ES2478872T3 (es) 2006-04-13 2007-04-12 Composición líquida que comprende una proteasa aspártica

Country Status (12)

Country Link
US (1) US20100009033A1 (es)
EP (2) EP2004820A1 (es)
JP (1) JP5557242B2 (es)
CN (1) CN101421397A (es)
BR (1) BRPI0709829A2 (es)
DK (1) DK2333056T3 (es)
EA (1) EA025412B1 (es)
ES (1) ES2478872T3 (es)
MX (1) MX2008013182A (es)
NZ (1) NZ571699A (es)
PL (1) PL2333056T3 (es)
WO (1) WO2007118838A1 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2617819B1 (en) 2006-09-21 2016-04-27 BASF Enzymes LLC Phytases, nucleic acids encoding them and methods for making and using them
AU2010215867B2 (en) 2009-02-20 2016-03-31 Danisco Us Inc. Fermentation broth formulations
CN101870967B (zh) * 2010-07-22 2012-05-23 安泰生物工程股份有限公司 半连续发酵生产微生物凝乳酶的方法
WO2012127005A1 (en) 2011-03-22 2012-09-27 Dsm Ip Assets B.V. Chymosin formulation
CN103773751A (zh) * 2012-10-22 2014-05-07 丰益(上海)生物技术研发中心有限公司 脂肪酶稳定剂
ES2685402T3 (es) 2013-07-18 2018-10-08 Chr. Hansen A/S Composición de enzima proteasa aspártica para coagulación de leche
CN103740685B (zh) * 2013-10-11 2015-04-15 孟乙 利用米黑毛霉生产凝乳酶的方法
CN104480095A (zh) * 2014-11-27 2015-04-01 广西大学 一种酶活力稳定的液态木瓜蛋白酶制剂
JP2017029129A (ja) * 2015-07-30 2017-02-09 三菱化学フーズ株式会社 プロテアーゼの活性維持方法およびプロテアーゼ溶液
WO2017089613A1 (en) 2015-11-26 2017-06-01 Csk Food Enrichment B.V. Liquid composition comprising an aspartic protease having improved enzyme stability
GB201520912D0 (en) 2015-11-26 2016-01-13 Csk Food Enrichment Bv Liquid composition comprising an aspartic protease having improved enzyme stability

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK110031A (es) 1965-12-02
US3763010A (en) * 1971-06-10 1973-10-02 Baxter Laboratories Inc Stabilized microbial rennet
US4348482A (en) * 1978-05-22 1982-09-07 Miles Laboratories, Inc. Process for decreasing the thermal stability of microbial rennet
IL87258A (en) 1987-07-28 1994-02-27 Gist Brocades Nv SECYMOREVYULK surrogate cells and their use for the expression of polypeptides
US4853329A (en) * 1988-06-27 1989-08-01 Miles Inc. Stabilization of microbial rennet
JP2974763B2 (ja) 1989-06-13 1999-11-10 シーエイチアール ハンセン エイエス キモシンの回収および精製
DK47493D0 (da) 1993-04-27 1993-04-27 Novo Nordisk As Fremgangsmaade til fremstilling af ost
JP2739704B2 (ja) * 1993-08-11 1998-04-15 アサヒビール株式会社 飲食品添加用液状パパイン組成物
ATE288482T1 (de) * 1994-05-03 2005-02-15 Hansens Lab Verfahren zur trennung milchgerinnender enzyme
JPH1169973A (ja) * 1997-08-29 1999-03-16 Kao Corp 酵素の安定化方法
JP2002153269A (ja) * 2000-11-24 2002-05-28 Cosmo Products Kk プロテアーゼ溶液の安定化方法および皮膚化粧料
SE0004808D0 (sv) 2000-12-20 2000-12-20 Apbiotech Ab Method for the purification of an enzyme
US7955829B2 (en) * 2001-12-19 2011-06-07 Dsm Ip Assets B.V. Method for the inactivation of amylase in the presence of protease
EP1365019B1 (en) 2002-05-21 2006-07-26 DSM IP Assets B.V. Fermentative production of microbial milk clotting protease
ATE408681T1 (de) 2002-05-29 2008-10-15 Dsm Ip Assets Bv Verfahren zur reinigung einer rhizomucor aspartat-protease

Also Published As

Publication number Publication date
BRPI0709829A2 (pt) 2011-07-26
EP2004820A1 (en) 2008-12-24
EP2333056B1 (en) 2014-05-07
JP2009533036A (ja) 2009-09-17
WO2007118838A1 (en) 2007-10-25
US20100009033A1 (en) 2010-01-14
DK2333056T3 (da) 2014-08-04
EA025412B1 (ru) 2016-12-30
EA200802140A1 (ru) 2009-02-27
NZ571699A (en) 2011-07-29
EP2333056A1 (en) 2011-06-15
JP5557242B2 (ja) 2014-07-23
MX2008013182A (es) 2008-10-27
PL2333056T3 (pl) 2014-09-30
CN101421397A (zh) 2009-04-29

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