ES2352633B8 - Cepa mutante de lactococcus lactis lactis y método para la producción industrial de acetoína. - Google Patents
Cepa mutante de lactococcus lactis lactis y método para la producción industrial de acetoína. Download PDFInfo
- Publication number
- ES2352633B8 ES2352633B8 ES200930561A ES200930561A ES2352633B8 ES 2352633 B8 ES2352633 B8 ES 2352633B8 ES 200930561 A ES200930561 A ES 200930561A ES 200930561 A ES200930561 A ES 200930561A ES 2352633 B8 ES2352633 B8 ES 2352633B8
- Authority
- ES
- Spain
- Prior art keywords
- lactis
- acetoin
- strain
- glucose
- cml
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- ROWKJAVDOGWPAT-UHFFFAOYSA-N Acetoin Chemical compound CC(O)C(C)=O ROWKJAVDOGWPAT-UHFFFAOYSA-N 0.000 title abstract 13
- 241000194041 Lactococcus lactis subsp. lactis Species 0.000 title abstract 2
- 235000014969 Streptococcus diacetilactis Nutrition 0.000 title 1
- 238000009776 industrial production Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- GFAZHVHNLUBROE-UHFFFAOYSA-N hydroxymethyl propionaldehyde Natural products CCC(=O)CO GFAZHVHNLUBROE-UHFFFAOYSA-N 0.000 abstract 6
- 238000000855 fermentation Methods 0.000 abstract 4
- 230000004151 fermentation Effects 0.000 abstract 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 3
- 239000008103 glucose Substances 0.000 abstract 3
- 241000894006 Bacteria Species 0.000 abstract 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 abstract 2
- 238000004519 manufacturing process Methods 0.000 abstract 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 1
- 102000003855 L-lactate dehydrogenase Human genes 0.000 abstract 1
- 108700023483 L-lactate dehydrogenases Proteins 0.000 abstract 1
- 241000194035 Lactococcus lactis Species 0.000 abstract 1
- 229910052799 carbon Inorganic materials 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 235000014655 lactic acid Nutrition 0.000 abstract 1
- 239000004310 lactic acid Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
- C12P7/26—Ketones
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La presente invención se refiere a la obtención de una bacteria ácido láctica mutante, la cepa L. lactis lactis CML B4, que dispone de una elevada capacidad de producir el compuesto químico acetoína. La bacteria mencionada presenta una actividad enzimática lactato deshidrogenasa reducida, inferior al 10% de la actividad de la cepa madre. Como consecuencia de ello, dicha cepa mutante produce una cantidad de acetoína más de diez veces superior a la producida por la cepa madre, de tipo silvestre, cuando ambas son cultivadas en idénticas condiciones en un medio de cultivo que contiene 10 g/L de glucosa como fuente de carbono. La presente invención se refiere también al desarrollo de un procedimiento eficiente de producción de acetoína mediante fermentación en condiciones aeróbicas basado en la utilización de la cepa de la invención. En las condiciones óptimas de fermentación que originan la máxima producción de acetoína la cepa L. lactis subsp. lactis CML B4 produce hasta 43,06 g de acetoína a partir de 100 g de glucosa en fermentaciones tipo discontinuo (batch), y 72,42 g de acetoína a partir de 200 g de glucosa en fermentaciones tipo semicontinuo (fed-batch).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200930561A ES2352633B8 (es) | 2009-08-04 | 2009-08-04 | Cepa mutante de lactococcus lactis lactis y método para la producción industrial de acetoína. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200930561A ES2352633B8 (es) | 2009-08-04 | 2009-08-04 | Cepa mutante de lactococcus lactis lactis y método para la producción industrial de acetoína. |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| ES2352633A1 ES2352633A1 (es) | 2011-02-22 |
| ES2352633B1 ES2352633B1 (es) | 2012-01-02 |
| ES2352633B8 true ES2352633B8 (es) | 2012-02-20 |
Family
ID=43557971
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES200930561A Active ES2352633B8 (es) | 2009-08-04 | 2009-08-04 | Cepa mutante de lactococcus lactis lactis y método para la producción industrial de acetoína. |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2352633B8 (es) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016142419A1 (en) | 2015-03-09 | 2016-09-15 | Fundacion Tecnalia Research & Innovation | Metabolite production by lactic acid bacterium |
| ES2859490T3 (es) * | 2016-07-19 | 2021-10-04 | Fundacion Tecnalia Res & Innovation | Cepa bacteriana productora de 2,3-butanodiol y otros metabolitos |
| WO2018050695A1 (en) | 2016-09-14 | 2018-03-22 | Biosyncaucho, S.L. | Electrochemical method for manufacturing methyl ethyl ketone |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2901008B2 (ja) * | 1989-11-28 | 1999-06-02 | 明治乳業株式会社 | 乳酸菌によるジアセチル、アセトイン発酵法 |
| DE69735633T3 (de) * | 1996-08-22 | 2010-04-15 | Chr. Hansen A/S | Metabolisch veränderte milchsäurebakterien und ihre verwendung |
| FR2777905B1 (fr) * | 1998-04-23 | 2002-07-19 | Agronomique Inst Nat Rech | Bacteries surproductrices d'alpha-acetolactate, et procede d'obtention |
-
2009
- 2009-08-04 ES ES200930561A patent/ES2352633B8/es active Active
Also Published As
| Publication number | Publication date |
|---|---|
| ES2352633B1 (es) | 2012-01-02 |
| ES2352633A1 (es) | 2011-02-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Nguyen et al. | Production of L-lactic acid from a green microalga, Hydrodictyon reticulum, by Lactobacillus paracasei LA104 isolated from the traditional Korean food, makgeolli | |
| Chatzifragkou et al. | Production of 1, 3-propanediol by Clostridium butyricum growing on biodiesel-derived crude glycerol through a non-sterilized fermentation process | |
| Abdel-Rahman et al. | Improved lactic acid productivity by an open repeated batch fermentation system using Enterococcus mundtii QU 25 | |
| Zeidan et al. | Stable coexistence of two Caldicellulosiruptor species in a de novo constructed hydrogen-producing co-culture | |
| De Giorgi et al. | Potential use of ricotta cheese whey for the production of lactobionic acid by Pseudomonas taetrolens strains | |
| JP5892507B2 (ja) | ３−ヒドロキシ酪酸又はその塩の産生方法。 | |
| KR101576186B1 (ko) | 에탄올 생산 경로가 봉쇄된 클루이베로마이세스 막시아누스 균주 및 이의 용도 | |
| MX2009010229A (es) | Nuevos microorganismos para la produccion de 1,2-propanodiol obtenido mediante una combinacion de evolucion iy diseño racional. | |
| FR2923491A1 (fr) | Utilisation de bacteries pour la production de sources de bioenergie | |
| MX2018012297A (es) | Metodo para la produccion de acido lactico. | |
| DK1776451T3 (da) | Fremgangsmåde til fremstilling af L-aminosyrer under anvendelse af stammer fra familien enterobacteriaceae | |
| TW200902721A (en) | Method for producing lactic acid | |
| Tongpim et al. | Isolation of thermophilic l-lactic acid producing bacteria showing homo-fermentative manner under high aeration condition | |
| Leite et al. | Canonical analysis technique as an approach to determine optimal conditions for lactic acid production by lactobacillus helveticus ATCC 15009 | |
| Tian et al. | The supplement of vitamin C facilitates L-lactic acid biosynthesis in Lactobacillus thermophilus A69 from sweet sorghum juice coupled with soybean hydrolysate as feedstocks | |
| WO2010143323A1 (ja) | 新規なｄ－乳酸生産菌及びｄ－乳酸を生産する方法 | |
| de Assis et al. | High cell density culture of dairy propionibacterium sp. and acidipropionibacterium sp.: A review for food industry applications | |
| ES2352633B8 (es) | Cepa mutante de lactococcus lactis lactis y método para la producción industrial de acetoína. | |
| WO2010084995A3 (en) | Process for producing l-lysine or l-threonine employing bacteria of the enterobacteriacea family in a culture medium with controlled glycerol concentration | |
| RU2012145469A (ru) | Способ получения зеаксантина ферментацией | |
| JP5717119B2 (ja) | Ｌ−乳酸の製造方法 | |
| KR20220169808A (ko) | 카프로익산 생산 미생물군집 제조방법 및 이의 용도 | |
| KR101224854B1 (ko) | 내당성 및 내산성이 향상된 락토바실러스 파라카제이 변이주 및 그의 용도 | |
| JP6145583B2 (ja) | 高効率エタノール発酵菌 | |
| AU2016288656A8 (en) | Process for producing lactic acid or its salts from fermentation using thermotolerance bacillus bacteria |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
Ref document number: 2352633 Country of ref document: ES Kind code of ref document: B1 Effective date: 20120102 |
|
| PC2A | Transfer of patent |
Owner name: FUNDACION TECNALIA RESEARCH & INNOVATION Effective date: 20120522 |