ES2352633B8 - Cepa mutante de lactococcus lactis lactis y método para la producción industrial de acetoína. - Google Patents
Cepa mutante de lactococcus lactis lactis y método para la producción industrial de acetoína. Download PDFInfo
- Publication number
- ES2352633B8 ES2352633B8 ES200930561A ES200930561A ES2352633B8 ES 2352633 B8 ES2352633 B8 ES 2352633B8 ES 200930561 A ES200930561 A ES 200930561A ES 200930561 A ES200930561 A ES 200930561A ES 2352633 B8 ES2352633 B8 ES 2352633B8
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- ES
- Spain
- Prior art keywords
- lactis
- acetoin
- strain
- glucose
- cml
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
- C12P7/26—Ketones
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La presente invención se refiere a la obtención de una bacteria ácido láctica mutante, la cepa L. lactis lactis CML B4, que dispone de una elevada capacidad de producir el compuesto químico acetoína. La bacteria mencionada presenta una actividad enzimática lactato deshidrogenasa reducida, inferior al 10% de la actividad de la cepa madre. Como consecuencia de ello, dicha cepa mutante produce una cantidad de acetoína más de diez veces superior a la producida por la cepa madre, de tipo silvestre, cuando ambas son cultivadas en idénticas condiciones en un medio de cultivo que contiene 10 g/L de glucosa como fuente de carbono. La presente invención se refiere también al desarrollo de un procedimiento eficiente de producción de acetoína mediante fermentación en condiciones aeróbicas basado en la utilización de la cepa de la invención. En las condiciones óptimas de fermentación que originan la máxima producción de acetoína la cepa L. lactis subsp. lactis CML B4 produce hasta 43,06 g de acetoína a partir de 100 g de glucosa en fermentaciones tipo discontinuo (batch), y 72,42 g de acetoína a partir de 200 g de glucosa en fermentaciones tipo semicontinuo (fed-batch).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200930561A ES2352633B8 (es) | 2009-08-04 | 2009-08-04 | Cepa mutante de lactococcus lactis lactis y método para la producción industrial de acetoína. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200930561A ES2352633B8 (es) | 2009-08-04 | 2009-08-04 | Cepa mutante de lactococcus lactis lactis y método para la producción industrial de acetoína. |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| ES2352633A1 ES2352633A1 (es) | 2011-02-22 |
| ES2352633B1 ES2352633B1 (es) | 2012-01-02 |
| ES2352633B8 true ES2352633B8 (es) | 2012-02-20 |
Family
ID=43557971
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES200930561A Active ES2352633B8 (es) | 2009-08-04 | 2009-08-04 | Cepa mutante de lactococcus lactis lactis y método para la producción industrial de acetoína. |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2352633B8 (es) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016142419A1 (en) | 2015-03-09 | 2016-09-15 | Fundacion Tecnalia Research & Innovation | Metabolite production by lactic acid bacterium |
| CN109563471B (zh) * | 2016-07-19 | 2023-02-14 | 腾亚研创 | 产生2,3-丁二醇和其他代谢物的细菌菌株 |
| US20190194814A1 (en) | 2016-09-14 | 2019-06-27 | Biosyncaucho, S.L. | Electrochemical method for manufacturing methyl ethyl ketone |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2901008B2 (ja) * | 1989-11-28 | 1999-06-02 | 明治乳業株式会社 | 乳酸菌によるジアセチル、アセトイン発酵法 |
| DK0928333T4 (da) * | 1996-08-22 | 2010-01-18 | Chr Hansen As | Metabolsk optimerede mælkesyrebakterier og deres anvendelse |
| FR2777905B1 (fr) * | 1998-04-23 | 2002-07-19 | Agronomique Inst Nat Rech | Bacteries surproductrices d'alpha-acetolactate, et procede d'obtention |
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2009
- 2009-08-04 ES ES200930561A patent/ES2352633B8/es active Active
Also Published As
| Publication number | Publication date |
|---|---|
| ES2352633B1 (es) | 2012-01-02 |
| ES2352633A1 (es) | 2011-02-22 |
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| Date | Code | Title | Description |
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| FG2A | Definitive protection |
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| PC2A | Transfer of patent |
Owner name: FUNDACION TECNALIA RESEARCH & INNOVATION Effective date: 20120522 |