ES2241120T3 - Metodos para liofilizar celulas competentes. - Google Patents

Metodos para liofilizar celulas competentes.

Info

Publication number
ES2241120T3
ES2241120T3 ES98906440T ES98906440T ES2241120T3 ES 2241120 T3 ES2241120 T3 ES 2241120T3 ES 98906440 T ES98906440 T ES 98906440T ES 98906440 T ES98906440 T ES 98906440T ES 2241120 T3 ES2241120 T3 ES 2241120T3
Authority
ES
Spain
Prior art keywords
cells
competent
transformation efficiency
lyophilized
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES98906440T
Other languages
English (en)
Spanish (es)
Inventor
Joel A. Jessee
Frederic R. Bloom
Thuan Trinh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Application granted granted Critical
Publication of ES2241120T3 publication Critical patent/ES2241120T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/16—Physical preservation processes
    • A01N1/162—Temperature processes, e.g. following predefined temperature changes over time
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
ES98906440T 1997-02-12 1998-02-11 Metodos para liofilizar celulas competentes. Expired - Lifetime ES2241120T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US3972097P 1997-02-12 1997-02-12
US39720P 1997-02-12

Publications (1)

Publication Number Publication Date
ES2241120T3 true ES2241120T3 (es) 2005-10-16

Family

ID=21907025

Family Applications (1)

Application Number Title Priority Date Filing Date
ES98906440T Expired - Lifetime ES2241120T3 (es) 1997-02-12 1998-02-11 Metodos para liofilizar celulas competentes.

Country Status (8)

Country Link
US (3) US6960464B2 (cg-RX-API-DMAC7.html)
EP (1) EP1005529B1 (cg-RX-API-DMAC7.html)
JP (1) JP2001512310A (cg-RX-API-DMAC7.html)
AT (1) ATE294229T1 (cg-RX-API-DMAC7.html)
DE (1) DE69829976T2 (cg-RX-API-DMAC7.html)
DK (1) DK1005529T3 (cg-RX-API-DMAC7.html)
ES (1) ES2241120T3 (cg-RX-API-DMAC7.html)
WO (1) WO1998035018A1 (cg-RX-API-DMAC7.html)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1005529B1 (en) * 1997-02-12 2005-04-27 Invitrogen Corporation Methods for lyophilizing competent cells
US6709852B1 (en) * 1999-06-22 2004-03-23 Invitrogen Corporation Rapid growing microorganisms for biotechnology applications
US20020081565A1 (en) * 2000-10-30 2002-06-27 Sigma-Aldrich Co. Process for producing freeze dried competent cells and use thereof in cloning
WO2002061111A2 (en) * 2000-12-15 2002-08-08 Stratagene Room temperature stable competent cells
US20040110267A1 (en) * 2000-12-15 2004-06-10 Stratagene Room temperature stable competent cells
US20040214306A1 (en) * 2003-01-23 2004-10-28 Bloom Fredric R. Rapid growing microorganisms for biotechnology applications
WO2004085613A2 (en) * 2003-03-19 2004-10-07 Stratagene Electrocompetent host cells
TWI414602B (zh) 2010-12-14 2013-11-11 Ind Tech Res Inst 新穎之大腸桿菌與其用途以及製備勝任細胞的方法
CN103387946A (zh) * 2012-05-11 2013-11-13 中国人民解放军军事医学科学院放射与辐射医学研究所 一种感受态宿主细胞的制备方法以及一种快速高效将外源物质转入宿主细胞的方法及其应用
US20150267210A1 (en) * 2012-10-16 2015-09-24 Genebridges Gmbh Electrocompetent cells and preparation thereof
KR102160389B1 (ko) 2013-08-05 2020-09-28 트위스트 바이오사이언스 코포레이션 드 노보 합성된 유전자 라이브러리
US9752829B2 (en) * 2014-01-07 2017-09-05 Sudhir R. Brahmbhatt Liquid nitrogen (LIN) integrated lyophilization system for minimizing a carbon footprint
WO2016014307A1 (en) * 2014-07-21 2016-01-28 New England Biolabs, Inc. Compositions and methods for extending storage time of competent cells at -20 c
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
CA2975855C (en) 2015-02-04 2025-09-23 Twist Bioscience Corporation SYNTHETIC GENE COMPOSITIONS AND ASSEMBLY METHODS
WO2016172377A1 (en) 2015-04-21 2016-10-27 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
KR20180050411A (ko) 2015-09-18 2018-05-14 트위스트 바이오사이언스 코포레이션 올리고핵산 변이체 라이브러리 및 그의 합성
WO2017053450A1 (en) 2015-09-22 2017-03-30 Twist Bioscience Corporation Flexible substrates for nucleic acid synthesis
EP3384077A4 (en) 2015-12-01 2019-05-08 Twist Bioscience Corporation FUNCTIONALIZED SURFACES AND MANUFACTURE THEREOF
WO2018038772A1 (en) 2016-08-22 2018-03-01 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
EP4664304A3 (en) 2016-09-21 2026-01-21 Atlas Data Storage, Inc. Nucleic acid based data storage
US10463065B2 (en) * 2016-12-01 2019-11-05 Council Of Scientific And Industrial Research Enzyme-assisted extraction of steviol glycosides from the leaves of Stevia rebaudiana Bertoni
GB2573069A (en) 2016-12-16 2019-10-23 Twist Bioscience Corp Variant libraries of the immunological synapse and synthesis thereof
WO2018156792A1 (en) 2017-02-22 2018-08-30 Twist Bioscience Corporation Nucleic acid based data storage
CA3056388A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
IL271205B2 (en) 2017-06-12 2025-02-01 Twist Bioscience Corp Methods for assembling continuous nucleic acids
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
WO2019126562A1 (en) * 2017-12-22 2019-06-27 Bio-Rad Laboratories, Inc. Controlling phenotype of organisms with crispr/cas gene targeting
IL312616B2 (en) 2018-01-04 2025-08-01 Twist Bioscience Corp Dna-based digital information storage
EP3814497A4 (en) 2018-05-18 2022-03-02 Twist Bioscience Corporation POLYNUCLEOTIDES, REAGENTS AND METHODS FOR NUCLEIC ACID HYBRIDIZATION
EP3902816A4 (en) 2018-12-26 2022-09-14 Twist Bioscience Corporation HIGH-PRECISION DE NOVO POLYNUCLEOTIDE SYNTHESIS
CA3131689A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for glp1 receptor
KR20210144698A (ko) 2019-02-26 2021-11-30 트위스트 바이오사이언스 코포레이션 항체 최적화를 위한 변이 핵산 라이브러리
EP3987019A4 (en) 2019-06-21 2023-04-19 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
CN115023440B (zh) 2019-09-23 2025-09-12 特韦斯特生物科学公司 结合CD3ε的抗体
US12091777B2 (en) 2019-09-23 2024-09-17 Twist Bioscience Corporation Variant nucleic acid libraries for CRTH2
AU2020400030B2 (en) 2019-12-09 2026-05-21 Twist Bioscience Corporation Variant nucleic acid libraries for adenosine receptors
CN113817609A (zh) * 2021-11-01 2021-12-21 苏州科正环保科技有限公司 基因工程菌的冻干保藏方法

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3843453A (en) 1970-11-27 1974-10-22 Miles Lab Process and composition for use in microbiological quality assurance
US4038143A (en) 1973-10-29 1977-07-26 The Regents Of The University Of Michigan Test kit for the genetic detection of microorganisms
DE3128411A1 (de) 1980-07-18 1983-09-15 Unisearch Ltd., 2033 Kensington, New South Wales Kulturstamm-mutante von esccherichia coli sowie verfahren zur herstellung von phenylalanin
US4446230A (en) 1981-10-30 1984-05-01 Temple University Of The Commonwealth System Of Higher Education Test method for the laboratory diagnosis of Gonorrhea and test strain of neisseria gonorrhoeae
US4520019A (en) 1982-04-29 1985-05-28 Ribi Immunochem Research, Inc. Stable composition and preparation thereof
JPS60258125A (ja) 1984-06-06 1985-12-20 Hayashibara Biochem Lab Inc 蛋白性生理活性物質を含有する水溶性乾燥物
DE3515586A1 (de) 1985-04-30 1986-11-06 Boehringer Mannheim Gmbh, 6800 Mannheim Stabilisiertes sarcosinoxidase-praeparat
JPH0779694B2 (ja) 1985-07-09 1995-08-30 カドラント バイオリソ−シズ リミテツド 蛋白質および同類品の保護
US4981797A (en) 1985-08-08 1991-01-01 Life Technologies, Inc. Process of producing highly transformable cells and cells produced thereby
US4851348A (en) 1986-07-16 1989-07-25 Cold Spring Harbor Laboratory Biologically pure escherichia coli cell line which is a deoR- mutant and which is more transformation efficient with foreign plasmids than deoR+ escherichia coli cell lines, processes for obtaining these cell lines, methods of use
US5292507A (en) 1986-08-01 1994-03-08 Imperial Oil Limited Method of using polysaccharides to stabilize microorganisms for inoculating plant seeds
FR2625221B1 (fr) 1987-12-24 1990-06-15 Sanofi Sa Protoplastes stabilises et procede pour leur obtention
US4808404A (en) 1988-01-11 1989-02-28 A. H. Robins Company, Inc. Live vaccine for coccidiosis utilizing coccidial sporozoites
US5178884A (en) 1988-05-18 1993-01-12 Cryopharm Corporation Lyophilized and reconstituted red blood cell compositions
US5958670A (en) 1988-05-18 1999-09-28 Cobe Laboratories, Inc. Method of freezing cells and cell-like materials
US5153004A (en) 1989-06-02 1992-10-06 Cryopharm Corporation Freezing and thawing of erythrocytes
US5213814A (en) 1989-04-10 1993-05-25 Cryopharm Corporation Lyophilized and reconstituted blood platelet compositions
US5043261A (en) 1989-04-10 1991-08-27 Cryopharm Corporation Lyophilized and reconstituted red blood cell and hemosome compositions
US5045446A (en) 1988-08-26 1991-09-03 Cryopharm Corporation Lyophilization of cells
US5059518A (en) 1988-10-20 1991-10-22 Coulter Corporation Stabilized lyophilized mammalian cells and method of making same
GB8903593D0 (en) 1989-02-16 1989-04-05 Pafra Ltd Storage of materials
US5149656A (en) 1990-05-03 1992-09-22 University Of Florida Research Foundation, Inc. Microbiological assay pad and kit for selective detection of toxicants
US5200399A (en) * 1990-09-14 1993-04-06 Boyce Thompson Institute For Plant Research, Inc. Method of protecting biological materials from destructive reactions in the dry state
US5733774A (en) * 1995-02-02 1998-03-31 Ecoscience Corporation Method and composition for producing stable bacteria and bacterial formulations
US6274369B1 (en) 1996-02-02 2001-08-14 Invitrogen Corporation Method capable of increasing competency of bacterial cell transformation
US5891692A (en) 1996-03-29 1999-04-06 Life Technologies, Inc. Method for increasing the viability and transformation ability of bacteria during or after storage at low temperatures
EP1005529B1 (en) * 1997-02-12 2005-04-27 Invitrogen Corporation Methods for lyophilizing competent cells
US6383810B2 (en) 1997-02-14 2002-05-07 Invitrogen Corporation Dry powder cells and cell culture reagents and methods of production thereof
US6610531B1 (en) * 1998-09-24 2003-08-26 The United States Of America As Represented By The Secretary Of The Navy Viable dried bacteria produced by drying in the presence of trehalose and divalent cation
US20030044965A1 (en) * 1998-09-24 2003-03-06 Alfred Mateczun Long term preservation and storage of viable dried bacteria
US20020081565A1 (en) * 2000-10-30 2002-06-27 Sigma-Aldrich Co. Process for producing freeze dried competent cells and use thereof in cloning
US6872357B1 (en) * 2000-11-22 2005-03-29 Quadrant Drug Delivery Limited Formulation of preservation mixtures containing sensitive biologicals to be stabilized for ambient temperature storage by drying
WO2002061111A2 (en) * 2000-12-15 2002-08-08 Stratagene Room temperature stable competent cells
AU2002248178A1 (en) * 2000-12-15 2002-08-12 Stratagene Mutant cells with enhanced resistance to desiccation
US20040110267A1 (en) * 2000-12-15 2004-06-10 Stratagene Room temperature stable competent cells
US20050074867A1 (en) * 2001-05-07 2005-04-07 Victor Bronshtein Preservation of competent cells at ambient temperatures

Also Published As

Publication number Publication date
US20060014255A1 (en) 2006-01-19
EP1005529A4 (en) 2002-06-12
ATE294229T1 (de) 2005-05-15
JP2001512310A (ja) 2001-08-21
DK1005529T3 (da) 2005-06-13
EP1005529A1 (en) 2000-06-07
US7648832B2 (en) 2010-01-19
DE69829976D1 (de) 2005-06-02
US20020164771A1 (en) 2002-11-07
EP1005529B1 (en) 2005-04-27
WO1998035018A1 (en) 1998-08-13
US6960464B2 (en) 2005-11-01
DE69829976T2 (de) 2006-04-27
US20100081171A1 (en) 2010-04-01

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