ES2194834T3 - Transformacion por medio de particulas de celulas somaticas animales. - Google Patents
Transformacion por medio de particulas de celulas somaticas animales.Info
- Publication number
- ES2194834T3 ES2194834T3 ES90910074T ES90910074T ES2194834T3 ES 2194834 T3 ES2194834 T3 ES 2194834T3 ES 90910074 T ES90910074 T ES 90910074T ES 90910074 T ES90910074 T ES 90910074T ES 2194834 T3 ES2194834 T3 ES 2194834T3
- Authority
- ES
- Spain
- Prior art keywords
- animals
- transformation
- somatic cell
- spark discharge
- cell particles
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
- C12N15/895—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection using biolistic methods
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Sustainable Development (AREA)
- Electromagnetism (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Medicinal Preparation (AREA)
Abstract
SE DESCUBRE UN METODO PARA LA TRANSFORMACION CONVENIENTE DE LAS CELULAS SOMATICAS DE ANIMALES. LA TRANSFORMACION DE CELULAS SOMATICAS ES UTIL PARA EL CUIDADO MEDICO Y VETERINARIO DE ENFERMEDADES GENETICAS, Y PARA OTROS PROPOSITOS TERAPEUTICOS O DE MEJORAMIENTO DE ANIMALES. EL METODO UTILIZA UN APARATO ELECTRICO DE ACELERACION DE DESCARGA DE PARTICULAS QUE PUEDE INYECTAR UNAS PARTICULAS MUY PEQUEÑAS DE ORO Y OTRO MATERIAL DENSO LLEVANDO CONSTRUCTORES GENETICOS REVESTIDOS EN EL A LAS CELULAS VIVAS DE ANIMALES. LOS ANIMALES NO SOLO VIVEN, SI NO QUE NO HAY NINGUN ROCE NI SANGRE EN EL LUGAR DEL TRATAMIENTO. EL METODO ES PARCICULARMENTE ADAPTABLE DESDE QUE LA FUERZA DE LA INYECCION DE PARTICULAS EN TAL APARATO DE DESCARGA DE CHISPAS ES AJUSTABLE MEDIANTE AJUSTES A LA TENSION DE LA DESCARGA DE CHISPAS.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US37186989A | 1989-06-26 | 1989-06-26 | |
US49493390A | 1990-03-14 | 1990-03-14 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2194834T3 true ES2194834T3 (es) | 2003-12-01 |
Family
ID=27005554
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES90910074T Expired - Lifetime ES2194834T3 (es) | 1989-06-26 | 1990-06-21 | Transformacion por medio de particulas de celulas somaticas animales. |
Country Status (10)
Country | Link |
---|---|
US (2) | US20020081739A1 (es) |
EP (1) | EP0431135B1 (es) |
JP (2) | JP3573454B2 (es) |
AT (1) | ATE235558T1 (es) |
AU (2) | AU5856790A (es) |
CA (1) | CA2019676C (es) |
DE (1) | DE69034053T2 (es) |
DK (1) | DK0431135T3 (es) |
ES (1) | ES2194834T3 (es) |
WO (1) | WO1991000359A1 (es) |
Families Citing this family (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1991007487A1 (en) | 1989-11-16 | 1991-05-30 | Duke University | Particle-mediated transformation of animal tissue cells |
US5149655A (en) * | 1990-06-21 | 1992-09-22 | Agracetus, Inc. | Apparatus for genetic transformation |
AU1663392A (en) * | 1991-03-06 | 1992-10-06 | Agracetus, Inc. | Particle mediated transformation of cotton |
CA2098498A1 (en) * | 1991-10-15 | 1993-04-16 | Ning-Sun Yang | Particle-mediated transformation of mammalian unattached cells |
WO1993008292A1 (en) * | 1991-10-16 | 1993-04-29 | Agracetus, Inc. | Particle-mediated transformation of animal somatic cells |
US5260191A (en) * | 1992-01-30 | 1993-11-09 | Agracetus, Inc. | Method for diagnosing tumors |
EP0584339A4 (en) * | 1992-03-04 | 1996-02-28 | Jon A Wolff | Method of delivering therapeutic substances to the brain |
DE69333814T2 (de) * | 1992-03-11 | 2006-02-02 | Powderject Vaccines, Inc., Madison | Genetischer impfstoff gegen den immunschwäche virus |
AU2146092A (en) * | 1992-05-28 | 1993-12-30 | Scientific Dimensions Usa, Inc. | Transgenic animal production with biolistically transformed spermatozoa |
AU7102194A (en) * | 1993-06-30 | 1995-01-24 | Dcv Biologics L.P. | A method for introducing a biological substance into a target |
GB2282139A (en) * | 1993-09-24 | 1995-03-29 | Univ Reading | Introducing DNA into the germ line of birds |
US6995008B1 (en) | 1994-03-07 | 2006-02-07 | Merck & Co., Inc. | Coordinate in vivo gene expression |
US7223739B1 (en) | 1995-06-07 | 2007-05-29 | Powderject Vaccines, Inc. | Adjuvanted genetic vaccines |
CA2216308A1 (en) * | 1995-12-21 | 1997-07-03 | Solvay (Societe Anonyme) | Plasmid vaccine for pseudorabies virus |
BE1010344A3 (fr) * | 1996-06-12 | 1998-06-02 | Solvay | Vaccin plasmidique contre le virus pseudorabique. |
US6693087B1 (en) | 1998-08-20 | 2004-02-17 | Aventis Pasteur Limited | Nucleic acid molecules encoding POMP91A protein of Chlamydia |
CA2340330A1 (en) | 1998-08-20 | 2000-03-02 | Aventis Pasteur Limited | Nucleic acid molecules encoding inclusion membrane protein c of chlamydia |
US6686339B1 (en) | 1998-08-20 | 2004-02-03 | Aventis Pasteur Limited | Nucleic acid molecules encoding inclusion membrane protein C of Chlamydia |
US6881723B1 (en) | 1998-11-05 | 2005-04-19 | Powderject Vaccines, Inc. | Nucleic acid constructs |
AU3593200A (en) | 1999-02-09 | 2000-08-29 | Powderject Vaccines, Inc. | (mycobacterium tuberculosis), immunization |
US7196066B1 (en) | 1999-11-03 | 2007-03-27 | Powderject Vaccines, Inc. | DNA-vaccines based on constructs derived from the genomes of human and animal pathogens |
EP1792995A3 (en) | 2000-05-08 | 2007-06-13 | Sanofi Pasteur Limited | Chlamydia secretory locus orf and uses thereof |
AU5810201A (en) | 2000-05-10 | 2001-11-20 | Aventis Pasteur | Immunogenic polypeptides encoded by mage minigenes and uses thereof |
GB0201736D0 (en) * | 2002-01-25 | 2002-03-13 | Glaxo Group Ltd | DNA dosage forms |
ES2285087T3 (es) * | 2002-01-25 | 2007-11-16 | Glaxo Group Limited | Formas farmaceuticas de adn. |
EP1356820A1 (en) | 2002-04-26 | 2003-10-29 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | DNA vaccine combined with an inducer of tumor cell apoptosis |
US9029135B2 (en) | 2009-03-27 | 2015-05-12 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Kanamycin antisense nucleic acid for the treatment of cancer |
CA2798837A1 (en) | 2010-06-07 | 2011-12-15 | Pfizer Inc. | Her-2 peptides and vaccines |
CN113260697A (zh) | 2018-12-20 | 2021-08-13 | 国立大学法人丰桥技术科学大学 | 电穿孔装置以及外来物质导入细胞的制造方法 |
IT201900007060A1 (it) | 2019-05-21 | 2020-11-21 | St Superiore Di Sanita | Cellule tumorali ingegnerizzate e loro usi |
IT201900012540A1 (it) | 2019-07-22 | 2021-01-22 | Humanitas Mirasole Spa | Inibitori di CHI3L1 e loro usi |
EP4200452A1 (en) | 2020-08-18 | 2023-06-28 | Enviro Metals, LLC | Metal refinement |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4945050A (en) * | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
IL84459A (en) * | 1986-12-05 | 1993-07-08 | Agracetus | Apparatus and method for the injection of carrier particles carrying genetic material into living cells |
US5015580A (en) * | 1987-07-29 | 1991-05-14 | Agracetus | Particle-mediated transformation of soybean plants and lines |
US5149655A (en) * | 1990-06-21 | 1992-09-22 | Agracetus, Inc. | Apparatus for genetic transformation |
US5405779A (en) * | 1993-04-09 | 1995-04-11 | Agracetus, Inc. | Apparatus for genetic transformation |
US5506125A (en) * | 1993-12-22 | 1996-04-09 | Agracetus, Inc. | Gene delivery instrument with replaceable cartridges |
-
1990
- 1990-06-21 AT AT90910074T patent/ATE235558T1/de not_active IP Right Cessation
- 1990-06-21 DE DE69034053T patent/DE69034053T2/de not_active Revoked
- 1990-06-21 WO PCT/US1990/003522 patent/WO1991000359A1/en active IP Right Grant
- 1990-06-21 DK DK90910074T patent/DK0431135T3/da active
- 1990-06-21 JP JP50925090A patent/JP3573454B2/ja not_active Expired - Lifetime
- 1990-06-21 AU AU58567/90A patent/AU5856790A/en not_active Abandoned
- 1990-06-21 ES ES90910074T patent/ES2194834T3/es not_active Expired - Lifetime
- 1990-06-21 EP EP90910074A patent/EP0431135B1/en not_active Revoked
- 1990-06-22 CA CA002019676A patent/CA2019676C/en not_active Expired - Lifetime
-
1994
- 1994-03-18 AU AU57873/94A patent/AU679477B2/en not_active Expired
-
2000
- 2000-12-20 JP JP2000387953A patent/JP2001213807A/ja not_active Withdrawn
-
2001
- 2001-12-20 US US10/032,316 patent/US20020081739A1/en not_active Abandoned
-
2005
- 2005-03-23 US US11/086,657 patent/US20050216959A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
AU679477B2 (en) | 1997-07-03 |
JP3573454B2 (ja) | 2004-10-06 |
AU5787394A (en) | 1994-05-19 |
EP0431135A1 (en) | 1991-06-12 |
DK0431135T3 (da) | 2003-07-21 |
AU5856790A (en) | 1991-01-17 |
JP2001213807A (ja) | 2001-08-07 |
CA2019676C (en) | 2005-08-09 |
DE69034053T2 (de) | 2003-12-04 |
US20020081739A1 (en) | 2002-06-27 |
JPH04500314A (ja) | 1992-01-23 |
WO1991000359A1 (en) | 1991-01-10 |
ATE235558T1 (de) | 2003-04-15 |
EP0431135B1 (en) | 2003-03-26 |
DE69034053D1 (de) | 2003-04-30 |
CA2019676A1 (en) | 1990-12-26 |
US20050216959A1 (en) | 2005-09-29 |
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