ES2192949B1 - Disminucion de la virulencia de mycobacterium tuberculosis por la inactivacion del gen phop. - Google Patents
Disminucion de la virulencia de mycobacterium tuberculosis por la inactivacion del gen phop.Info
- Publication number
- ES2192949B1 ES2192949B1 ES200101824A ES200101824A ES2192949B1 ES 2192949 B1 ES2192949 B1 ES 2192949B1 ES 200101824 A ES200101824 A ES 200101824A ES 200101824 A ES200101824 A ES 200101824A ES 2192949 B1 ES2192949 B1 ES 2192949B1
- Authority
- ES
- Spain
- Prior art keywords
- phop
- virulence
- mutant
- mycobacterium tuberculosis
- gen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
- A61P31/06—Antibacterial agents for tuberculosis
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Communicable Diseases (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Oncology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Pulmonology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
La construcción de un mutante por recombinación homóloga a partir de un aislado clínico de Mycobacterium tuberculosis en el que se inactiva el gen phoP, atenúa su virulencia en el macrófago de médula ósea de ratón. El mutante phoP también disminuye su virulencia en el modelo experimental de ratón. Este mutante phoP es además capaz de persistir sin ser eliminado tanto en el macrófago como en el ratón.
Priority Applications (10)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES200101824A ES2192949B1 (es) | 2001-07-31 | 2001-07-31 | Disminucion de la virulencia de mycobacterium tuberculosis por la inactivacion del gen phop. |
DE60229962T DE60229962D1 (de) | 2001-07-31 | 2002-07-30 | Verringerung der virulenz von mycobacterium tuberculosis und tuberkuloseschutz mittels inaktivierung des phop-gens |
PT02751195T PT1428870E (pt) | 2001-07-31 | 2002-07-30 | Redução da virulência de mycobacterium tuberculosis e protecção contra a tuberculose pela inactivação do gene phop |
CA2456007A CA2456007C (en) | 2001-07-31 | 2002-07-30 | Reduction in the virulence of mycobacterium tuberculosis and protection against tuberculosis by means of phop gene inactivation |
US10/485,372 US7468190B2 (en) | 2001-07-31 | 2002-07-30 | Reduction in the virulence of Mycobacterium tuberculosis and protection against tuberculosis by means of phoP gene inactivation |
EP02751195A EP1428870B1 (en) | 2001-07-31 | 2002-07-30 | Reduction in the virulence of mycobacterium tuberculosis and protection against tuberculosis by means of phop gene inactivation |
PCT/ES2002/000381 WO2003012075A1 (es) | 2001-07-31 | 2002-07-30 | DISMINUCIÓN DE VIRULENCIA DE MYCOBACTERIUM TUBERCULOSIS Y PROTECCIÓN CONTRA LA TUBERCULOSIS POR LA INACTIVACIÓN DEL GEN phoP |
ES02751195T ES2316590T3 (es) | 2001-07-31 | 2002-07-30 | Reduccion de la virulencia de mycobacterium tuberculosis y proteccion frente a la tuberculosis mediante la inactivacion del gen phop. |
JP2003517253A JP4153423B2 (ja) | 2001-07-31 | 2002-07-30 | ミコバクテリウム結核菌の毒力の減少およびphoP遺伝子の不活性化による結核菌に対する保護 |
AT02751195T ATE414761T1 (de) | 2001-07-31 | 2002-07-30 | Verringerung der virulenz von mycobacterium tuberculosis und tuberkuloseschutz mittels inaktivierung des phop-gens |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES200101824A ES2192949B1 (es) | 2001-07-31 | 2001-07-31 | Disminucion de la virulencia de mycobacterium tuberculosis por la inactivacion del gen phop. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2192949A1 ES2192949A1 (es) | 2003-10-16 |
ES2192949B1 true ES2192949B1 (es) | 2004-08-16 |
Family
ID=8498623
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES200101824A Expired - Fee Related ES2192949B1 (es) | 2001-07-31 | 2001-07-31 | Disminucion de la virulencia de mycobacterium tuberculosis por la inactivacion del gen phop. |
ES02751195T Expired - Lifetime ES2316590T3 (es) | 2001-07-31 | 2002-07-30 | Reduccion de la virulencia de mycobacterium tuberculosis y proteccion frente a la tuberculosis mediante la inactivacion del gen phop. |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES02751195T Expired - Lifetime ES2316590T3 (es) | 2001-07-31 | 2002-07-30 | Reduccion de la virulencia de mycobacterium tuberculosis y proteccion frente a la tuberculosis mediante la inactivacion del gen phop. |
Country Status (9)
Country | Link |
---|---|
US (1) | US7468190B2 (es) |
EP (1) | EP1428870B1 (es) |
JP (1) | JP4153423B2 (es) |
AT (1) | ATE414761T1 (es) |
CA (1) | CA2456007C (es) |
DE (1) | DE60229962D1 (es) |
ES (2) | ES2192949B1 (es) |
PT (1) | PT1428870E (es) |
WO (1) | WO2003012075A1 (es) |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2433518T3 (es) * | 2006-03-24 | 2013-12-11 | Universidad De Zaragoza | Vacuna contra la tuberculosis |
WO2009025888A2 (en) * | 2007-05-10 | 2009-02-26 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Regulated synthesis of antigen and/or regulated attentuation to enhance vaccine immunogenics and/or safety |
WO2008141226A2 (en) * | 2007-05-10 | 2008-11-20 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Recombinant bacteria comprising vectors for expression of nucleic acid sequences encoding antigens |
WO2009046449A1 (en) * | 2007-10-05 | 2009-04-09 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Recombinant bacterium capable of eliciting an immune response against enteric pathogens |
WO2010045620A1 (en) | 2008-10-17 | 2010-04-22 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Recombinant bacterium capable of eliciting an immune response against streptococcus pneumoniae |
US9163219B2 (en) * | 2009-04-14 | 2015-10-20 | Arizona Board Of Regents On Behalf Of Arizona State University | Single expression vector for generation of a virus with a segmented genome |
WO2010135563A1 (en) | 2009-05-22 | 2010-11-25 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Recombinant bacterium and methods of antigen and nucleic acid delivery |
US9045742B2 (en) * | 2009-05-29 | 2015-06-02 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Recombinant Edwardsiella bacterium |
US9062297B2 (en) | 2010-01-13 | 2015-06-23 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Yersinia pestis vaccine |
WO2011091291A1 (en) * | 2010-01-22 | 2011-07-28 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | BACTERIUM COMPRISING A REGULATED rfaH NUCLEIC ACID |
CN102439134B (zh) * | 2010-04-22 | 2016-01-20 | 刘军 | 包含过表达PhoP和/或PhoP调节子蛋白的重组BCG菌株的结核疫苗 |
WO2011150421A2 (en) | 2010-05-28 | 2011-12-01 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Recombinant bacterium to decrease tumor growth |
US9303264B2 (en) | 2012-05-18 | 2016-04-05 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Photosynthetic microorganisms expressing thermostable lipase |
US9580718B2 (en) | 2013-06-17 | 2017-02-28 | Arizona Board Of Regents On Behalf Of Arizona State University | Attenuated live bacteria with increased acid resistance and methods of use thereof |
CN109423479A (zh) * | 2017-09-05 | 2019-03-05 | 江苏齐氏生物科技有限公司 | 一种大鼠骨髓巨噬细胞分离及培养方法 |
CN109810932A (zh) * | 2019-02-09 | 2019-05-28 | 济宁医学院 | 新疆地区的结核分枝杆菌临床分离株中存在PhoP基因的天然缺失突变菌株 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU645489B2 (en) * | 1989-03-31 | 1994-01-20 | Washington University | Vaccines containing avirulent phoP-type microorganisms |
US5695983A (en) | 1990-12-18 | 1997-12-09 | The General Hospital Corporation | Salmonella vaccines |
US5843426A (en) | 1990-12-18 | 1998-12-01 | The General Hospital Corporation | Salmonella vaccines |
-
2001
- 2001-07-31 ES ES200101824A patent/ES2192949B1/es not_active Expired - Fee Related
-
2002
- 2002-07-30 CA CA2456007A patent/CA2456007C/en not_active Expired - Fee Related
- 2002-07-30 ES ES02751195T patent/ES2316590T3/es not_active Expired - Lifetime
- 2002-07-30 DE DE60229962T patent/DE60229962D1/de not_active Expired - Lifetime
- 2002-07-30 WO PCT/ES2002/000381 patent/WO2003012075A1/es active Application Filing
- 2002-07-30 PT PT02751195T patent/PT1428870E/pt unknown
- 2002-07-30 JP JP2003517253A patent/JP4153423B2/ja not_active Expired - Fee Related
- 2002-07-30 EP EP02751195A patent/EP1428870B1/en not_active Expired - Lifetime
- 2002-07-30 AT AT02751195T patent/ATE414761T1/de active
- 2002-07-30 US US10/485,372 patent/US7468190B2/en not_active Expired - Lifetime
Non-Patent Citations (3)
Title |
---|
PÉREZ, E. et al. "An essential role for pho P in Mycobacterium tuberculosis virulence". MOLECULAR MICROBIOLOGY, 2001, Vol. 41, Nº 1, páginas 179-187. Todo el documento. * |
ZAHRT, T.C. et al. "An essential two-component signal transduction system in Mycobacterium tuberculosis". JOURNAL OF BACTERIOLOGY, 2000, Vol. 182, Nº 13, páginas 3832-3838. Todo el documento. * |
ZAHRT, T.C. et al. "Elements of signal transduction in Mycobacterium tuberculosis: In vitro phosphorylation an in vivo expression of the response regulator MtrA". JOURNAL OF BACTERIOLOGY, 1996, Vol. 178. * |
Also Published As
Publication number | Publication date |
---|---|
ES2192949A1 (es) | 2003-10-16 |
DE60229962D1 (de) | 2009-01-02 |
US20050106175A1 (en) | 2005-05-19 |
JP4153423B2 (ja) | 2008-09-24 |
EP1428870A1 (en) | 2004-06-16 |
JP2004535827A (ja) | 2004-12-02 |
CA2456007A1 (en) | 2003-02-13 |
CA2456007C (en) | 2013-10-08 |
EP1428870B1 (en) | 2008-11-19 |
PT1428870E (pt) | 2009-02-16 |
ATE414761T1 (de) | 2008-12-15 |
US7468190B2 (en) | 2008-12-23 |
ES2316590T3 (es) | 2009-04-16 |
WO2003012075A1 (es) | 2003-02-13 |
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Legal Events
Date | Code | Title | Description |
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EC2A | Search report published |
Date of ref document: 20031016 Kind code of ref document: A1 |
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FD2A | Announcement of lapse in spain |
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