ES2190491T3 - Procedimiento para la amplificacion de la señal de respuesta de un inmunoensayo tipo sandwich. - Google Patents
Procedimiento para la amplificacion de la señal de respuesta de un inmunoensayo tipo sandwich.Info
- Publication number
- ES2190491T3 ES2190491T3 ES97108863T ES97108863T ES2190491T3 ES 2190491 T3 ES2190491 T3 ES 2190491T3 ES 97108863 T ES97108863 T ES 97108863T ES 97108863 T ES97108863 T ES 97108863T ES 2190491 T3 ES2190491 T3 ES 2190491T3
- Authority
- ES
- Spain
- Prior art keywords
- amplification
- antibody
- procedure
- response signal
- marked
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/811—Test for named disease, body condition or organ function
- Y10S436/814—Pregnancy
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
SE DESCRIBE UNA MEJORA DE UN INMUNOENSAYO DE TIPO SANDWICH EN EL CUAL SE INMOVILIZA EN UN SOPORTE SOLIDO UN ANTICUERPO QUE ES ESPECIFICO PARA UN EPITOPO DEL ANALITO CUYA PRESENCIA O CONCENTRACION SE ESTA DETERMINANDO Y UN PRIMER ANTICUERPO MARCADO QUE ES ESPECIFICO PARA OTRO EPITOPO DEL ANALITO. LA MEJORA IMPLICA PROPORCIONAR UN SEGUNDO ANTICUERPO MARCADO QUE ES ESPECIFICO PARA EL PRIMER ANTICUERPO MARCADO PARA FORMAR DE ESTA FORMA UNA CADENA DE DOS O MAS ANTICUERPOS MARCADOS QUE RESULTA EN UNA AMPLIFICACION DE LA SEÑAL GENERADA AL FORMARSE EL SANDWICH.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/660,923 US5876944A (en) | 1996-06-10 | 1996-06-10 | Method for amplification of the response signal in a sandwich immunoassay |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2190491T3 true ES2190491T3 (es) | 2003-08-01 |
Family
ID=24651502
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES97108863T Expired - Lifetime ES2190491T3 (es) | 1996-06-10 | 1997-06-03 | Procedimiento para la amplificacion de la señal de respuesta de un inmunoensayo tipo sandwich. |
Country Status (7)
Country | Link |
---|---|
US (1) | US5876944A (es) |
EP (1) | EP0813064B1 (es) |
JP (1) | JP3758814B2 (es) |
AU (1) | AU714613B2 (es) |
CA (1) | CA2198958C (es) |
DE (1) | DE69718558T2 (es) |
ES (1) | ES2190491T3 (es) |
Families Citing this family (49)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6207651B1 (en) * | 1996-08-02 | 2001-03-27 | Metabolite Laboratories | Method for treatment and prevention of deficiencies of vitamins B12, folic acid, and B6 |
BE1010184A3 (fr) * | 1996-04-30 | 1998-02-03 | Biocode Sa | Procede de detection et/ou de quantification d'un haptene dans une phase homogene et dispositif pour sa mise en oeuvre. |
US20030235877A1 (en) * | 1996-04-30 | 2003-12-25 | La Region Wallone | Conjugates of haptens to beta-lactam derivatives and their use for detecting and/or quantifying haptens in solution and device for implementation thereof |
US6436721B1 (en) * | 1997-07-25 | 2002-08-20 | Bayer Corporation | Device and method for obtaining clinically significant analyte ratios |
US6753190B1 (en) * | 1998-07-01 | 2004-06-22 | Nitto Denko Corporation | Immunologic test method and immunologic test kit |
JP2000310638A (ja) * | 1999-04-28 | 2000-11-07 | Fuji Photo Film Co Ltd | 均一系酵素免疫分析方法 |
DE10006432A1 (de) * | 2000-02-14 | 2001-08-16 | Ganzimmun Inst Fuer Ganzheitli | Verfahren zum Nachweis von Helicobacter pylori in Stuhl- und Speichelproben |
US6908741B1 (en) * | 2000-05-30 | 2005-06-21 | Transtech Pharma, Inc. | Methods to identify compounds that modulate RAGE |
GB0029154D0 (en) | 2000-11-30 | 2001-01-17 | Lee Helen | Signal enhancement with multiple labelled-antibodies |
US6600057B2 (en) | 2000-12-29 | 2003-07-29 | Kimberly-Clark Worldwide, Inc. | Matrix metalloproteinase inhibitors |
US7041787B2 (en) * | 2000-12-29 | 2006-05-09 | Kimberly-Clark Worldwide, Inc. | Design and use of advanced zinc chelating peptides to regulate matrix metalloproteinases |
US20050266499A1 (en) * | 2001-04-25 | 2005-12-01 | Rockeby Biomed Corporation, Ltd. | Method and apparatus for testing for presence or absence of select biological substances |
US20030119073A1 (en) * | 2001-12-21 | 2003-06-26 | Stephen Quirk | Sensors and methods of detection for proteinase enzymes |
US8367013B2 (en) * | 2001-12-24 | 2013-02-05 | Kimberly-Clark Worldwide, Inc. | Reading device, method, and system for conducting lateral flow assays |
US20030119203A1 (en) * | 2001-12-24 | 2003-06-26 | Kimberly-Clark Worldwide, Inc. | Lateral flow assay devices and methods for conducting assays |
US6837171B1 (en) | 2002-04-29 | 2005-01-04 | Palmer/Snyder Furniture Company | Lightweight table with unitized table top |
JP3634307B2 (ja) * | 2001-12-27 | 2005-03-30 | 三菱電機株式会社 | チャット管理システム |
US7432105B2 (en) * | 2002-08-27 | 2008-10-07 | Kimberly-Clark Worldwide, Inc. | Self-calibration system for a magnetic binding assay |
US7285424B2 (en) | 2002-08-27 | 2007-10-23 | Kimberly-Clark Worldwide, Inc. | Membrane-based assay devices |
US7314763B2 (en) * | 2002-08-27 | 2008-01-01 | Kimberly-Clark Worldwide, Inc. | Fluidics-based assay devices |
US7781172B2 (en) * | 2003-11-21 | 2010-08-24 | Kimberly-Clark Worldwide, Inc. | Method for extending the dynamic detection range of assay devices |
US20040121334A1 (en) * | 2002-12-19 | 2004-06-24 | Kimberly-Clark Worldwide, Inc. | Self-calibrated flow-through assay devices |
US7247500B2 (en) * | 2002-12-19 | 2007-07-24 | Kimberly-Clark Worldwide, Inc. | Reduction of the hook effect in membrane-based assay devices |
US7585641B2 (en) * | 2003-01-21 | 2009-09-08 | Agdia, Inc. | Immunoassay and method of use |
US7851209B2 (en) * | 2003-04-03 | 2010-12-14 | Kimberly-Clark Worldwide, Inc. | Reduction of the hook effect in assay devices |
US20040197819A1 (en) * | 2003-04-03 | 2004-10-07 | Kimberly-Clark Worldwide, Inc. | Assay devices that utilize hollow particles |
US7943395B2 (en) * | 2003-11-21 | 2011-05-17 | Kimberly-Clark Worldwide, Inc. | Extension of the dynamic detection range of assay devices |
US7713748B2 (en) * | 2003-11-21 | 2010-05-11 | Kimberly-Clark Worldwide, Inc. | Method of reducing the sensitivity of assay devices |
US20050112703A1 (en) * | 2003-11-21 | 2005-05-26 | Kimberly-Clark Worldwide, Inc. | Membrane-based lateral flow assay devices that utilize phosphorescent detection |
US20050136500A1 (en) * | 2003-12-19 | 2005-06-23 | Kimberly-Clark Worldwide; Inc. | Flow-through assay devices |
US20050136550A1 (en) * | 2003-12-19 | 2005-06-23 | Kimberly-Clark Worldwide, Inc. | Flow control of electrochemical-based assay devices |
US7943089B2 (en) * | 2003-12-19 | 2011-05-17 | Kimberly-Clark Worldwide, Inc. | Laminated assay devices |
US20050191704A1 (en) * | 2004-03-01 | 2005-09-01 | Kimberly-Clark Worldwide, Inc. | Assay devices utilizing chemichromic dyes |
US7094528B2 (en) * | 2004-06-30 | 2006-08-22 | Kimberly-Clark Worldwide, Inc. | Magnetic enzyme detection techniques |
US7906276B2 (en) | 2004-06-30 | 2011-03-15 | Kimberly-Clark Worldwide, Inc. | Enzymatic detection techniques |
US7521226B2 (en) | 2004-06-30 | 2009-04-21 | Kimberly-Clark Worldwide, Inc. | One-step enzymatic and amine detection technique |
CN101300490A (zh) * | 2005-08-23 | 2008-11-05 | 反应生医公司 | 多重指向性免疫层析分析 |
BRPI0717416A2 (pt) | 2006-09-21 | 2013-11-12 | Prometheus Lab Inc | Método para realizar um imunoensaio complexo de alta produtividade, e, arranjo |
WO2011008990A1 (en) | 2009-07-15 | 2011-01-20 | Prometheus Laboratories Inc. | Drug selection for gastric cancer therapy using antibody-based arrays |
US9250243B2 (en) * | 2006-09-21 | 2016-02-02 | Nestec S.A. | Drug selection for lung cancer therapy using antibody-based arrays |
ES2398618T3 (es) | 2008-02-25 | 2013-03-20 | Nestec S.A. | Selección de fármacos para terapia del cáncer de mama utilizando matrices de anticuerpos |
US20090263905A1 (en) * | 2008-04-18 | 2009-10-22 | Kim Scheuringer | Detection test assembly for detecting the presence of a substance in a sample |
AU2010325893B2 (en) * | 2009-12-04 | 2014-05-29 | Rapid Pathogen Screening, Inc. | Multiplanar lateral flow assay with sample compressor |
JP5132664B2 (ja) * | 2009-12-07 | 2013-01-30 | 富士フイルム株式会社 | イムノクロマトグラフ方法 |
US9719995B2 (en) | 2011-02-03 | 2017-08-01 | Pierian Holdings, Inc. | Drug selection for colorectal cancer therapy using receptor tyrosine kinase profiling |
ES2553456T3 (es) | 2011-09-02 | 2015-12-09 | Nestec S.A. | Perfilado de proteínas de ruta de señal para determinar una eficacia terapéutica |
CA3092717A1 (en) * | 2012-02-21 | 2013-08-29 | Laboratory Corporation Of America Holdings | Methods and systems for signal amplification of bioassays |
USD860674S1 (en) | 2018-02-06 | 2019-09-24 | San Jamar, Inc. | Towel dispenser |
JP7369017B2 (ja) * | 2018-11-26 | 2023-10-25 | デンカ株式会社 | コロイド粒子を用いた被検物質検出増感法 |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4474892A (en) * | 1983-02-16 | 1984-10-02 | Board Of Trustees Of The Leland Stanford Junior University | Two-site immunoassays using monoclonal antibodies of different classes or subclasses and test kits for performing same |
US4703017C1 (en) * | 1984-02-14 | 2001-12-04 | Becton Dickinson Co | Solid phase assay with visual readout |
US4657853A (en) * | 1984-09-14 | 1987-04-14 | E. I. Du Pont De Nemours And Company | Immunoassays utilizing covalent conjugates of polymerized enzyme and antibody |
WO1988008534A1 (en) * | 1987-04-27 | 1988-11-03 | Unilever Plc | Immunoassays and devices therefor |
US5120643A (en) * | 1987-07-13 | 1992-06-09 | Abbott Laboratories | Process for immunochromatography with colloidal particles |
US4853335A (en) * | 1987-09-28 | 1989-08-01 | Olsen Duane A | Colloidal gold particle concentration immunoassay |
US5102788A (en) * | 1988-11-21 | 1992-04-07 | Hygeia Sciences, Inc. | Immunoassay including lyophilized reactant mixture |
US6083689A (en) * | 1990-10-16 | 2000-07-04 | Bayer Corporation | Sensitive immunoassays utilizing antibody conjugates with replicable DNA templates |
JPH05322893A (ja) * | 1991-05-11 | 1993-12-07 | B M L:Kk | 免疫学的測定用bf分離法 |
JPH04350559A (ja) * | 1991-05-28 | 1992-12-04 | Dai Ichi Pure Chem Co Ltd | 特異抗体の測定法 |
US5326707A (en) * | 1991-11-29 | 1994-07-05 | Miles Inc. | Composition and device for urinary protein assay and method of using the same |
-
1996
- 1996-06-10 US US08/660,923 patent/US5876944A/en not_active Expired - Lifetime
-
1997
- 1997-03-03 CA CA002198958A patent/CA2198958C/en not_active Expired - Fee Related
- 1997-06-03 ES ES97108863T patent/ES2190491T3/es not_active Expired - Lifetime
- 1997-06-03 DE DE69718558T patent/DE69718558T2/de not_active Expired - Lifetime
- 1997-06-03 EP EP97108863A patent/EP0813064B1/en not_active Expired - Lifetime
- 1997-06-06 AU AU24753/97A patent/AU714613B2/en not_active Ceased
- 1997-06-09 JP JP15059297A patent/JP3758814B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
US5876944A (en) | 1999-03-02 |
AU714613B2 (en) | 2000-01-06 |
CA2198958A1 (en) | 1997-12-10 |
JPH1062419A (ja) | 1998-03-06 |
CA2198958C (en) | 2003-12-30 |
EP0813064B1 (en) | 2003-01-22 |
DE69718558D1 (de) | 2003-02-27 |
AU2475397A (en) | 1997-12-18 |
DE69718558T2 (de) | 2003-08-28 |
JP3758814B2 (ja) | 2006-03-22 |
EP0813064A1 (en) | 1997-12-17 |
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