ES2189869T3 - Procedimiento para el enriquecimiento de poblaciones de celulas raras. - Google Patents
Procedimiento para el enriquecimiento de poblaciones de celulas raras.Info
- Publication number
- ES2189869T3 ES2189869T3 ES96910382T ES96910382T ES2189869T3 ES 2189869 T3 ES2189869 T3 ES 2189869T3 ES 96910382 T ES96910382 T ES 96910382T ES 96910382 T ES96910382 T ES 96910382T ES 2189869 T3 ES2189869 T3 ES 2189869T3
- Authority
- ES
- Spain
- Prior art keywords
- erythrocytes
- blood sample
- fetal nucleated
- enrichment
- procedure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/491—Blood by separating the blood components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
- B01D17/02—Separation of non-miscible liquids
- B01D17/0202—Separation of non-miscible liquids by ab- or adsorption
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
- B01D17/02—Separation of non-miscible liquids
- B01D17/0217—Separation of non-miscible liquids by centrifugal force
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
- B01D17/06—Separation of liquids from each other by electricity
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
- B01D17/08—Thickening liquid suspensions by filtration
- B01D17/085—Thickening liquid suspensions by filtration with membranes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D21/00—Separation of suspended solid particles from liquids by sedimentation
- B01D21/26—Separation of sediment aided by centrifugal force or centripetal force
- B01D21/262—Separation of sediment aided by centrifugal force or centripetal force by using a centrifuge
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
- B01D17/02—Separation of non-miscible liquids
- B01D17/04—Breaking emulsions
- B01D17/047—Breaking emulsions with separation aids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2221/00—Applications of separation devices
- B01D2221/10—Separation devices for use in medical, pharmaceutical or laboratory applications, e.g. separating amalgam from dental treatment residues
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Thermal Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Ecology (AREA)
- Electrochemistry (AREA)
- Biophysics (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
SE PRESENTA UN METODO PARA ENRIQUECER UNA MUESTRA DE SANGRE TOTAL CON CELULAS ESCASAS. UNA MUESTRA DE SANGRE (12) SE DISPONE EN CAPAS EN FICOLL/HYPAQUE-1119 (14) Y SE CENTRIFUGA PARA ELIMINAR LOS ERITROCITOS MATERNOS (16) Y EL RESTO DE LA MUESTRA DE SANGRE CENTRIFUGADA SE HACE PASAR A TRAVES DE UN APARATO SEPARADOR ESTABILIZADO DE CARGA-FLUJO A CONTRACORRIENTE CONTROLADO POR LA GRAVEDAD (20) PARA SEPARAR LOS ERITROCITOS NUCLEADOS FETALES (26) DE LOS DEMAS COMPONENTES CELULARES MEDIANTE LA APLICACION DE UN CAMPO ELECTRICO. LOS ERITROCITOS NUCLEADOS FETALES SEPARADOS (26) SON SOMETIDOS A HEMOLISIS CON AMONIACO (30) PARA ELIMINAR LOS ERITROCITOS MADUROS Y RECUPERAR LOS ERITROCITOS NUCLEADOS FETALES ENRIQUECIDOS.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/401,131 US5676849A (en) | 1994-10-21 | 1995-03-08 | Method for enrichment of fetal cell population from maternal whole blood samples |
PCT/US1995/013631 WO1996012945A1 (en) | 1994-10-21 | 1995-10-20 | Method for separating nucleated fetal erythrocytes |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2189869T3 true ES2189869T3 (es) | 2003-07-16 |
Family
ID=23586432
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES96910382T Expired - Lifetime ES2189869T3 (es) | 1995-03-08 | 1996-03-07 | Procedimiento para el enriquecimiento de poblaciones de celulas raras. |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0813442B1 (es) |
JP (1) | JPH11502106A (es) |
AT (1) | ATE228026T1 (es) |
DE (1) | DE69624908T2 (es) |
ES (1) | ES2189869T3 (es) |
WO (1) | WO1996027420A1 (es) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2813891B1 (fr) * | 2000-09-14 | 2005-01-14 | Immunotech Sa | Reactif multifonctionnel pour erythrocytes mettant en jeu des carbamates et applications |
US8986944B2 (en) | 2001-10-11 | 2015-03-24 | Aviva Biosciences Corporation | Methods and compositions for separating rare cells from fluid samples |
US8980568B2 (en) | 2001-10-11 | 2015-03-17 | Aviva Biosciences Corporation | Methods and compositions for detecting non-hematopoietic cells from a blood sample |
EP1524321B2 (en) * | 2003-10-16 | 2014-07-23 | Sequenom, Inc. | Non-invasive detection of fetal genetic traits |
US20090305236A1 (en) * | 2005-05-11 | 2009-12-10 | Genetic Technologies Limited | Methods of enriching fetal cells |
EP2041299A4 (en) | 2006-07-14 | 2010-01-13 | Aviva Biosciences Corp | METHOD AND COMPOSITIONS FOR DETECTION OF RARE CELLS FROM A BIOLOGICAL SAMPLE |
US9334477B2 (en) | 2010-08-20 | 2016-05-10 | Japan Advanced Institute Of Science And Technology | Method for collecting nucleated red blood cells via density-gradient centrifugation utilizing changes in blood cell density |
ITRM20120028A1 (it) * | 2012-01-25 | 2013-07-26 | Natalia Malara | Metodo per isolare cellule tumorali circolanti. |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4925572A (en) * | 1987-10-20 | 1990-05-15 | Pall Corporation | Device and method for depletion of the leukocyte content of blood and blood components |
US5192553A (en) * | 1987-11-12 | 1993-03-09 | Biocyte Corporation | Isolation and preservation of fetal and neonatal hematopoietic stem and progenitor cells of the blood and methods of therapeutic use |
US5336387A (en) * | 1990-09-11 | 1994-08-09 | Bioseparations, Inc. | Electrical separator apparatus and method of counterflow gradient focusing |
US5439571A (en) * | 1991-11-19 | 1995-08-08 | Bioseparations, Inc. | Apparatus and method for enhanced resolution continuous flow zone electrophoresis |
US5457024A (en) * | 1993-01-22 | 1995-10-10 | Aprogenex, Inc. | Isolation of fetal erythrocytes |
US5432054A (en) * | 1994-01-31 | 1995-07-11 | Applied Imaging | Method for separating rare cells from a population of cells |
US5489386A (en) * | 1994-01-31 | 1996-02-06 | Applied Imaging | Density gradient medium for the separation of cells |
-
1996
- 1996-03-07 JP JP8527058A patent/JPH11502106A/ja not_active Ceased
- 1996-03-07 DE DE69624908T patent/DE69624908T2/de not_active Expired - Fee Related
- 1996-03-07 WO PCT/US1996/003165 patent/WO1996027420A1/en active IP Right Grant
- 1996-03-07 ES ES96910382T patent/ES2189869T3/es not_active Expired - Lifetime
- 1996-03-07 EP EP96910382A patent/EP0813442B1/en not_active Expired - Lifetime
- 1996-03-07 AT AT96910382T patent/ATE228026T1/de not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
JPH11502106A (ja) | 1999-02-23 |
ATE228026T1 (de) | 2002-12-15 |
EP0813442A4 (en) | 1998-09-09 |
WO1996027420A1 (en) | 1996-09-12 |
EP0813442A1 (en) | 1997-12-29 |
EP0813442B1 (en) | 2002-11-20 |
DE69624908T2 (de) | 2003-07-03 |
DE69624908D1 (de) | 2003-01-02 |
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