AU4259793A - Process for the prenatal diagnosis of genetic anomalies - Google Patents

Process for the prenatal diagnosis of genetic anomalies

Info

Publication number
AU4259793A
AU4259793A AU42597/93A AU4259793A AU4259793A AU 4259793 A AU4259793 A AU 4259793A AU 42597/93 A AU42597/93 A AU 42597/93A AU 4259793 A AU4259793 A AU 4259793A AU 4259793 A AU4259793 A AU 4259793A
Authority
AU
Australia
Prior art keywords
nucleated erythrocytes
pregnant women
various
blood
hybridisation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
AU42597/93A
Other versions
AU667723B2 (en
Inventor
Dorothee Ahlert
Hendrikus Stephanus Paulus Garritsen
Wolfgang Holzgreve
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Garritsen Hendrikus Stephanus Paulus
Original Assignee
Garritsen Hendrikus Stephanus Paulus
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Garritsen Hendrikus Stephanus Paulus filed Critical Garritsen Hendrikus Stephanus Paulus
Publication of AU4259793A publication Critical patent/AU4259793A/en
Application granted granted Critical
Publication of AU667723B2 publication Critical patent/AU667723B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analyzing Materials By The Use Of Ultrasonic Waves (AREA)

Abstract

It has been possible to demonstrate clearly both in umbilical cord blood samples and after the separation of pregnancy blood that the combination of the triple gradient and MACS with anti-CD71 marked cells is a very effective method of enriching nucleated erythrocytes. The proportion of nucleated erythrocytes in the umbilical cord blood was between 72 and 89 % after both enrichment methods. It has been possible to detect nucleated erythrocytes in the posivite fraction according to the triple gradient and MACS in all cases in pregnant women at various gestation stages. The variation in the number of enriched nucleated erythrocytes in various pregnancies very probably reflects individual differences in the foeto-maternal cell ratio at various stages in pregnancy. The process described here makes it possible in normal male blood and that of non-pregnant women to demonstrate no nucleated erythrocytes, by contrast with pregnant women at various stages of gestation. The method is also highly reproducible and suitable for clinical diagnosis. With fluorescence $i(in situ) hybridisation it has been possible to detect a foetal trisomia in all three cases investigated. The enrichment of nucleated erythrocytes is thus strong enough to diagnose infantile aneuploids using fluorescence $i(in situ) hybridisation. It is therefore obvious that it is possible with this only slightly invasive and relatively simple and economical method to conduct screening examinations for three of the most important trisomias (13, 18 and 21) and monogenic diseases (with the aid of the PCR) with virtually no risk to the patient and the child.
AU42597/93A 1992-05-16 1993-05-11 Process for the prenatal diagnosis of genetic anomalies Ceased AU667723B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DE4216345 1992-05-16
DE4216345 1992-05-16
DE4222573 1992-07-09
DE4222573A DE4222573C1 (en) 1992-05-16 1992-07-09
PCT/DE1993/000427 WO1993023754A1 (en) 1992-05-16 1993-05-11 Process for the prenatal diagnosis of genetic abnormalities

Publications (2)

Publication Number Publication Date
AU4259793A true AU4259793A (en) 1993-12-13
AU667723B2 AU667723B2 (en) 1996-04-04

Family

ID=25914908

Family Applications (1)

Application Number Title Priority Date Filing Date
AU42597/93A Ceased AU667723B2 (en) 1992-05-16 1993-05-11 Process for the prenatal diagnosis of genetic anomalies

Country Status (9)

Country Link
EP (1) EP0597068B1 (en)
JP (1) JPH06509178A (en)
AT (1) ATE163093T1 (en)
AU (1) AU667723B2 (en)
CA (1) CA2113194A1 (en)
DE (2) DE4222573C1 (en)
DK (1) DK0597068T3 (en)
ES (1) ES2115059T3 (en)
WO (1) WO1993023754A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5489386A (en) * 1994-01-31 1996-02-06 Applied Imaging Density gradient medium for the separation of cells
US5432054A (en) * 1994-01-31 1995-07-11 Applied Imaging Method for separating rare cells from a population of cells
GB9603249D0 (en) * 1996-02-16 1996-04-17 Univ Nottingham Foetal cell analysis
CA2283416A1 (en) * 1997-03-08 1998-09-17 The University Of Dundee Prenatal diagnostic methods
AU5967100A (en) * 1999-07-21 2001-02-13 Britta Christensen A novel method for the improved isolation of a target cell population
JP6234542B1 (en) 2016-12-27 2017-11-22 株式会社 ＴＬ Ｇｅｎｏｍｉｃｓ Method for obtaining chromosomal DNA derived from fetal cells
SG11202002907RA (en) * 2017-10-19 2020-05-28 Tl Genomics Inc Cell classification chip
JP2020103299A (en) * 2020-02-18 2020-07-09 株式会社 ＴＬ Ｇｅｎｏｍｉｃｓ Method for acquiring nucleic acid derived from fetal cell

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4434156A (en) * 1981-10-26 1984-02-28 The Salk Institute For Biological Studies Monoclonal antibodies specific for the human transferrin receptor glycoprotein
AU621694B2 (en) * 1988-12-06 1992-03-19 Flinders Technologies Pty Ltd Isolation of fetal cells from maternal blood to enable prenatal diagnosis
ATE161960T1 (en) * 1988-12-06 1998-01-15 Flinders Technologies Pty Ltd ISOLATION OF FETAL CELLS FROM MATERNAL BLOOD TO PERFORM PRENATAL DIAGNOSTICS
FR2657167B1 (en) * 1990-01-16 1992-05-07 Clonatec Sa METHOD FOR IN VITRO PRENATAL SCREENING FOR FETAL TRISOMY IN A BIOLOGICAL SAMPLE LIKELY CONTAINING HCG AND NECESSARY FOR ITS IMPLEMENTATION.

Also Published As

Publication number Publication date
DE4222573C1 (en) 1993-08-12
EP0597068A1 (en) 1994-05-18
DK0597068T3 (en) 1998-09-23
JPH06509178A (en) 1994-10-13
CA2113194A1 (en) 1993-11-25
AU667723B2 (en) 1996-04-04
DE59308101D1 (en) 1998-03-12
EP0597068B1 (en) 1998-02-04
WO1993023754A1 (en) 1993-11-25
ES2115059T3 (en) 1998-06-16
ATE163093T1 (en) 1998-02-15

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