ES2182501T3 - Metodos mejorados para producir proteinas del factor viii. - Google Patents

Metodos mejorados para producir proteinas del factor viii.

Info

Publication number
ES2182501T3
ES2182501T3 ES99912427T ES99912427T ES2182501T3 ES 2182501 T3 ES2182501 T3 ES 2182501T3 ES 99912427 T ES99912427 T ES 99912427T ES 99912427 T ES99912427 T ES 99912427T ES 2182501 T3 ES2182501 T3 ES 2182501T3
Authority
ES
Spain
Prior art keywords
improved methods
factor proteins
solution
produce factor
viii
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES99912427T
Other languages
English (en)
Inventor
Brian D Kelley
Johanna Dalborg
Anna Petersson
Venkata Suresh Vunnum
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genetics Institute LLC
Original Assignee
Genetics Institute LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetics Institute LLC filed Critical Genetics Institute LLC
Application granted granted Critical
Publication of ES2182501T3 publication Critical patent/ES2182501T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Un método mejorado para la purificación de un polipéptido del factor VIII que comprende: a) añadir una mezcla que contiene el polipéptido del factor VIII que se va a purificar a una matriz de inmunoafinidad que se une por atracción hidrofóbica al polipéptido del factor VIII; b) eluir el polipéptido del factor VIII desde la matriz de inmunoafinidad con una solución de desorción que causa la desorción del polipéptido del factor VIII, que se libera a una solución de elución; c) diluir la solución de elución con una solución que comprende mayor fuerza iónica que la de la solución de elución, dando como resultado una solución diluida del factor VIII, por lo que la solución de elución se diluye de 3 veces a 5 veces con respecto a la solución de elución inicial; d) hacer pasar la solución diluida de factor VIII a través de una columna de intercambio iónico capaz de unirse al polipéptido del factor VIII, por lo que se une el polipéptido del factor VIII mientras que se permite que los contaminantes pasen a través de la columna de intercambio iónico; y e) eluir el polipéptido del factor VIII purificado de la columna de intercambio iónico.
ES99912427T 1998-03-12 1999-03-11 Metodos mejorados para producir proteinas del factor viii. Expired - Lifetime ES2182501T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US7780298P 1998-03-12 1998-03-12

Publications (1)

Publication Number Publication Date
ES2182501T3 true ES2182501T3 (es) 2003-03-01

Family

ID=22140139

Family Applications (1)

Application Number Title Priority Date Filing Date
ES99912427T Expired - Lifetime ES2182501T3 (es) 1998-03-12 1999-03-11 Metodos mejorados para producir proteinas del factor viii.

Country Status (11)

Country Link
US (2) US6683159B2 (es)
EP (1) EP1062244B1 (es)
JP (1) JP4405668B2 (es)
AT (1) ATE222925T1 (es)
AU (1) AU747274C (es)
CA (1) CA2306820C (es)
DE (1) DE69902639T2 (es)
DK (1) DK1062244T3 (es)
ES (1) ES2182501T3 (es)
PT (1) PT1062244E (es)
WO (1) WO1999046299A1 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8183344B2 (en) 1996-04-24 2012-05-22 University Of Michigan Inactivation resistant factor VIII
WO1999046299A1 (en) * 1998-03-12 1999-09-16 Genetics Institute, Inc. Improved methods for producing factor viii proteins
US7820796B2 (en) * 1998-03-12 2010-10-26 Genetics Institute, Llc. Methods for producing Factor VIII proteins
US20050060775A1 (en) * 1998-05-14 2005-03-17 Hooker Brian S. Production of human coagulation factor VIII from plant cells and whole plants
US7498024B2 (en) 2003-06-03 2009-03-03 Cell Genesys, Inc. Compositions and methods for enhanced expression of immunoglobulins from a single vector using a peptide cleavage site
US7485291B2 (en) 2003-06-03 2009-02-03 Cell Genesys, Inc. Compositions and methods for generating multiple polypeptides from a single vector using a virus derived peptide cleavage site, and uses thereof
WO2008005847A2 (en) * 2006-06-30 2008-01-10 The Regents Of The University Of Michigan Method of producing factor viii proteins by recombinant methods
US20090203077A1 (en) * 2006-06-30 2009-08-13 The Regents Of The University Of Michigan Method of producing factor viii proteins by recombinant methods
AU2007347505B2 (en) 2007-02-23 2012-11-22 Sk Bioscience Co., Ltd. Process for producing and purifying factor VIII and its derivatives
CA2821711C (en) 2010-12-15 2017-10-10 Baxter International Inc. Eluate collection using conductivity gradient
RU2500818C1 (ru) * 2012-07-19 2013-12-10 Общество с ограниченной ответственностью "Международный биотехнологический центр "Генериум" ("МБЦ "Генериум") РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК рАР227, КОДИРУЮЩАЯ ПОЛИПЕПТИД РЕКОМБИНАНТНОГО ФАКТОРА VIII СВЕРТЫВАЕМОСТИ КРОВИ ЧЕЛОВЕКА, ЛИНИЯ КЛЕТОК Cricetulus griseus CHO 2H5 - ПРОДУЦЕНТ РЕКОМБИНАНТНОГО ФАКТОРА VIII СВЕРТЫВАЕМОСТИ КРОВИ ЧЕЛОВЕКА И СПОСОБ ПОЛУЧЕНИЯ ПОЛИПЕПТИДА, ОБЛАДАЮЩЕГО АКТИВНОСТЬЮ ФАКТОРА VIII
JP2014138614A (ja) * 2014-04-09 2014-07-31 Sk Chemicals Co Ltd 第ｖｉｉｉ因子とその誘導体の製造及び精製方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4312935A (en) * 1979-08-09 1982-01-26 International Business Machines Corporation Class of E-beam resists based on conducting organic charge transfer salts
JPS5764648A (en) * 1980-10-06 1982-04-19 Takeda Chem Ind Ltd N-substituted derivative of valienamine, its preparation, and alpha-glucosidase inhibitor
US4757006A (en) 1983-10-28 1988-07-12 Genetics Institute, Inc. Human factor VIII:C gene and recombinant methods for production
US4743680A (en) * 1985-02-01 1988-05-10 New York University Method for purifying antihemophilic factor
CA1341174C (en) 1985-04-12 2001-01-30 John J. Toole Jr. Procoagulant proteins derived from factor viii: c
DE3785102T2 (de) 1986-01-03 1993-07-22 Genetics Inst Verfahren zur herstellung von faktor-viii:c-typ-proteinen.
US5451521A (en) 1986-05-29 1995-09-19 Genetics Institute, Inc. Procoagulant proteins
US4912040A (en) 1986-11-14 1990-03-27 Genetics Institute, Inc. Eucaryotic expression system
CA1339946C (en) * 1987-03-31 1998-07-07 Michael J. Griffith Ultrapurification process for polypeptides
CA1331157C (en) 1987-04-06 1994-08-02 Randal J. Kaufman Method for producing factor viii:c-type proteins
WO1999046299A1 (en) * 1998-03-12 1999-09-16 Genetics Institute, Inc. Improved methods for producing factor viii proteins

Also Published As

Publication number Publication date
DE69902639T2 (de) 2003-04-24
AU3080399A (en) 1999-09-27
DK1062244T3 (da) 2002-12-30
EP1062244B1 (en) 2002-08-28
AU747274B2 (en) 2002-05-09
DE69902639D1 (de) 2002-10-02
JP4405668B2 (ja) 2010-01-27
ATE222925T1 (de) 2002-09-15
PT1062244E (pt) 2002-12-31
US20020115832A1 (en) 2002-08-22
CA2306820C (en) 2008-09-09
EP1062244A1 (en) 2000-12-27
US20040087776A1 (en) 2004-05-06
WO1999046299A1 (en) 1999-09-16
CA2306820A1 (en) 1999-09-16
AU747274C (en) 2004-03-18
US6683159B2 (en) 2004-01-27
JP2002506081A (ja) 2002-02-26

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