ES2179874T3 - Procedimiento y articulos para producir metabolitos secundarios de celulas vegetales viables inmovilizadas en una matriz porosa. - Google Patents

Procedimiento y articulos para producir metabolitos secundarios de celulas vegetales viables inmovilizadas en una matriz porosa.

Info

Publication number
ES2179874T3
ES2179874T3 ES95920202T ES95920202T ES2179874T3 ES 2179874 T3 ES2179874 T3 ES 2179874T3 ES 95920202 T ES95920202 T ES 95920202T ES 95920202 T ES95920202 T ES 95920202T ES 2179874 T3 ES2179874 T3 ES 2179874T3
Authority
ES
Spain
Prior art keywords
matrix
pct
sio2
cells
viable
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95920202T
Other languages
English (en)
Inventor
Elsa Mariella Cappelletti
Giovanni Carturan
Anna Piovan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Silbiotec Due SA
Original Assignee
Silbiotec Due SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Silbiotec Due SA filed Critical Silbiotec Due SA
Application granted granted Critical
Publication of ES2179874T3 publication Critical patent/ES2179874T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14Enzymes or microbial cells immobilised on or in an inorganic carrier
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/04Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres

Landscapes

  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Dispersion Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

SE DESCRIBE UN PROCEDIMIENTO PARA PRODUCIR METABOLITOS SECUNDARIOS DE CELULAS VEGETALES VIABLES, QUE INCLUYEN LAS ETAPAS DE: (A) PREPARAR UN SOPORTE QUE INCLUYE UNA MATRIZ SUSTANCIALMENTE UNIFORME Y POROSA DE MATERIAL INORGANICO, QUE POSEE UNA RESISTENCIA A LA TRACCION DE AL MENOS 500 MPA; (B) INTRODUCIR UN CULTIVO DE CELULAS VEGETALES VIABLES EN LOS POROS DE DICHA MATRIZ; (C) ATRAPAR LAS CELULAS VEGETALES MEDIANTE EL REVESTIMIENTO DE LA MATRIZ CON UN SOL O UNA SUSPENSION COLOIDAL QUE NO INTERFIERA CON LA VIABILIDAD DE LAS CELULAS; (D) INMOVILIZAR LAS CELULAS ATRAPADAS EN LA MATRIZ CON UN GAS REACTIVO QUE INCLUYE UN GAS VEHICULO SATURADO CON SIO SUB,2} VOLATIL O PRECURSORES DE SIO SUB,2} MODIFICADOS ORGANICOS. LA MATRIZ PUEDE SER UN SIO SUB,2} O MATRICES DE OXIDO INORGANICO, EN LAS CUALES LA PROPORCION EN PESO ENTRE LA CARGA CELULAR Y EL MATERIAL INORGANICO VARIA ENTRE 1 X 10 SUP,-4} Y 1 X 10 SUP,-2}. LAS CELULAS INMOVILIZADAS NO ENTRAN EN SOLUCION DURANTE UN PERIODO DE 6 MESES Y MANTIENENSU VIABILIDAD MIENTRAS QUE PRODUCEN METABOLITOS SECUNDARIOS.
ES95920202T 1995-05-18 1995-05-18 Procedimiento y articulos para producir metabolitos secundarios de celulas vegetales viables inmovilizadas en una matriz porosa. Expired - Lifetime ES2179874T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IT1995/000083 WO1996036703A1 (en) 1995-05-18 1995-05-18 Process and articles for producing secondary metabolites of viable plant cells immobilized in a porous matrix

Publications (1)

Publication Number Publication Date
ES2179874T3 true ES2179874T3 (es) 2003-02-01

Family

ID=11332468

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95920202T Expired - Lifetime ES2179874T3 (es) 1995-05-18 1995-05-18 Procedimiento y articulos para producir metabolitos secundarios de celulas vegetales viables inmovilizadas en una matriz porosa.

Country Status (11)

Country Link
US (1) US5998162A (es)
EP (1) EP0827535B1 (es)
JP (1) JP3662026B2 (es)
AT (1) ATE221572T1 (es)
AU (1) AU2574095A (es)
BR (1) BR9510598A (es)
CA (1) CA2219491A1 (es)
DE (1) DE69527657T2 (es)
ES (1) ES2179874T3 (es)
IL (1) IL118220A (es)
WO (1) WO1996036703A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6214593B1 (en) * 1996-05-28 2001-04-10 Biosil A.G. Encapsulation of supported animal cells using gas-phase inorganic alkoxides
DE10016554A1 (de) * 2000-04-03 2001-10-18 Rootec Ges Fuer Bioaktive Wirk Vorrichtung zum Kultivieren von pflanzlichen oder tierischen Gewebekulturen
DE10163640A1 (de) * 2001-12-21 2003-07-03 Arno Kromminga Nachweis von Wechselwirkungen zwischen mindestens zwei spezifischen Biomolekülen
CA2471820A1 (en) * 2002-01-18 2003-07-24 Gianni Carturan Bioreactor
WO2009158502A1 (en) * 2008-06-25 2009-12-30 Purdue Research Foundation Encapsulation of living cells within an aerosolized sol-gel matrix
US9677065B2 (en) 2009-07-21 2017-06-13 Purdue Research Foundation Cell-mediated silica sol-gel encapsulation of living cells and tissues
WO2012074502A1 (en) * 2010-11-29 2012-06-07 Chayil Technologies, Llc Secondary metabolite stimulation in photoautotrophic cultures
DE102013208227A1 (de) * 2013-05-06 2014-11-06 Siemens Aktiengesellschaft Photobioreaktor für immobilisierte Mikroorganismen
US11299700B1 (en) 2021-02-19 2022-04-12 Acequia Biotechnology, Llc Bioreactor containers and methods of growing hairy roots using the same

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4845054A (en) * 1985-06-14 1989-07-04 Focus Semiconductor Systems, Inc. Low temperature chemical vapor deposition of silicon dioxide films
GB2185998A (en) * 1986-01-30 1987-08-05 Pilkington Brothers Plc Improvements in or relating to the growth of plant tissue cultures
EP0267470A1 (en) * 1986-11-03 1988-05-18 Manville Corporation Porous glass fiber mats for attachment of cells and biologically active substances
US5041138A (en) * 1986-11-20 1991-08-20 Massachusetts Institute Of Technology Neomorphogenesis of cartilage in vivo from cell culture

Also Published As

Publication number Publication date
EP0827535B1 (en) 2002-07-31
EP0827535A1 (en) 1998-03-11
DE69527657D1 (de) 2002-09-05
BR9510598A (pt) 1999-11-30
IL118220A (en) 2000-07-16
AU2574095A (en) 1996-11-29
JP3662026B2 (ja) 2005-06-22
CA2219491A1 (en) 1996-11-21
WO1996036703A1 (en) 1996-11-21
DE69527657T2 (de) 2003-04-03
JPH11505120A (ja) 1999-05-18
ATE221572T1 (de) 2002-08-15
IL118220A0 (en) 1996-09-12
US5998162A (en) 1999-12-07

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