ES2173888T3 - Nueva proteasa. - Google Patents

Nueva proteasa.

Info

Publication number
ES2173888T3
ES2173888T3 ES93911969T ES93911969T ES2173888T3 ES 2173888 T3 ES2173888 T3 ES 2173888T3 ES 93911969 T ES93911969 T ES 93911969T ES 93911969 T ES93911969 T ES 93911969T ES 2173888 T3 ES2173888 T3 ES 2173888T3
Authority
ES
Spain
Prior art keywords
protease
glutamic acid
proteasa
new
prepropeptides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93911969T
Other languages
English (en)
Inventor
Etsuo Nakamura
Hiroshige Tsuzuki
Kengo Kitadokoro
Masaru Shin
Hiroshi Teraoka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shionogi and Co Ltd
Original Assignee
Shionogi and Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shionogi and Co Ltd filed Critical Shionogi and Co Ltd
Application granted granted Critical
Publication of ES2173888T3 publication Critical patent/ES2173888T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/886—Streptomyces
    • Y10S435/896—Streptomyces fradiae

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

SE PRESENTA UNA NUEVA PROTEASA QUE SEPARA EL C TERMINAL DEL RESIDUO DEL ACIDO GLUTAMICO EN LA SECUENCIA DE AMINOACIDO DE UN POLIPEPTIDO QUE SE ORIGINA EN EL "STREPTOMYCES FRADIAE", Y UN PROCESO PARA OBTENER LA PROTEASA A PARTIR DE ESTA BACTERIA. LA INVENCION HA CLARIFICADO LAS PROPIEDADES DE ESTA PROTEASA Y TAMBIEN UNA SECUENCIA DE DNA QUE CODIFICA ESTA PROTEASA Y LOS PREPROPEPTIDOS. LA PROTEASA ES ESPECIFICA PARA EL ACIDO GLUTAMICO Y PUEDE UTILIZARSE PARA DIFERENTES PROPOSITOS, TALES COMO EL ANALISIS DE LAS PROTEINAS Y LA DIVISION DE CADENAS DE PEPTIDOS EN LA ZONA DESEADA DE UNA PROTEINA DE FUSION.
ES93911969T 1992-05-19 1993-04-30 Nueva proteasa. Expired - Lifetime ES2173888T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP12651192 1992-05-19

Publications (1)

Publication Number Publication Date
ES2173888T3 true ES2173888T3 (es) 2002-11-01

Family

ID=14937022

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93911969T Expired - Lifetime ES2173888T3 (es) 1992-05-19 1993-04-30 Nueva proteasa.

Country Status (7)

Country Link
US (1) US5665586A (es)
EP (1) EP0647710B1 (es)
JP (1) JP3396033B2 (es)
AT (1) ATE214731T1 (es)
DE (1) DE69331742T2 (es)
ES (1) ES2173888T3 (es)
WO (1) WO1993023530A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5616485A (en) * 1993-12-23 1997-04-01 Cangene Corporation Streptomyces proteases and improved streptomyces strains for expression of peptides and polypeptides
ES2111497B1 (es) * 1996-07-24 1998-09-01 Univ Madrid Complutense Procedimiento enzimatico para la disociacion de celulas de tejidos animales y su aplicacion.
US6855548B2 (en) 2000-02-08 2005-02-15 F. Hoffman-La Roche Ag Use of acid-stable proteases in animal feed
US6727277B1 (en) 2002-11-12 2004-04-27 Kansas State University Research Foundation Compounds affecting cholesterol absorption
WO2004072279A2 (en) * 2003-02-07 2004-08-26 Novozymes A/S Proteases
WO2004072221A2 (en) 2003-02-07 2004-08-26 Novozymes A/S Proteases
CA2539693A1 (en) 2003-10-10 2005-04-21 Novozymes A/S Protease variants
CN101014704A (zh) 2004-06-21 2007-08-08 诺维信公司 蛋白酶

Also Published As

Publication number Publication date
ATE214731T1 (de) 2002-04-15
US5665586A (en) 1997-09-09
DE69331742T2 (de) 2002-11-14
EP0647710A1 (en) 1995-04-12
EP0647710B1 (en) 2002-03-20
DE69331742D1 (de) 2002-04-25
JP3396033B2 (ja) 2003-04-14
WO1993023530A1 (en) 1993-11-25
EP0647710A4 (en) 1996-06-26

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