ES2172801T3 - Procedimiento y dispositivo para la purificacion y enriquecimiento de moleculas. - Google Patents
Procedimiento y dispositivo para la purificacion y enriquecimiento de moleculas.Info
- Publication number
- ES2172801T3 ES2172801T3 ES97931688T ES97931688T ES2172801T3 ES 2172801 T3 ES2172801 T3 ES 2172801T3 ES 97931688 T ES97931688 T ES 97931688T ES 97931688 T ES97931688 T ES 97931688T ES 2172801 T3 ES2172801 T3 ES 2172801T3
- Authority
- ES
- Spain
- Prior art keywords
- molecules
- enrichment
- purification
- procedure
- dissolution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title abstract 2
- 238000000746 purification Methods 0.000 title abstract 2
- 238000004090 dissolution Methods 0.000 abstract 3
- 239000002253 acid Substances 0.000 abstract 1
- 150000007513 acids Chemical class 0.000 abstract 1
- 230000005684 electric field Effects 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/24—Extraction; Separation; Purification by electrochemical means
- C07K1/26—Electrophoresis
Abstract
LA INVENCION TRATA DE UN PROCEDIMIENTO PARA LA PURIFICACION Y ENRIQUECIMIENTO DE PRIMERAS MOLECULAS (9) QUE ESTAN CARGADAS, COMO PROTEINAS, ACIDOS NUCLEICOS Y SIMILARES, QUE COMPRENDE LAS SIGUIENTES ETAPAS: A) PREPARACION DE UNA DISOLUCION QUE CONTENGA LAS PRIMERAS MOLECULAS (9), B) PONER EN CONTACTO LA DISOLUCION CON UN ELECTRODO (2), EL CUAL LLEVA DIRECTAMENTE UNA CAPA QUE TIENE UNA AFINIDAD CON LAS SEGUNDAS MOLECULAS (4) QUE CONTIENEN LAS PRIMERAS MOLECULAS (9), Y C) UNIR EL ELECTRODO (2) CON UN MEDIO (11) PARA FORMAR UN CAMPO ELECTRICO, DE TAL MANERA QUE OCURRA EN LA DISOLUCION UN MOVIMIENTO DE LAS PRIMERAS MOLECULAS (9) DIRIGIDA HACIA EL ELECTRODO (2).
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19628171A DE19628171C2 (de) | 1996-07-12 | 1996-07-12 | Verfahren und Vorrichtung zur Aufreinigung und Anreicherung von Molekülen |
DE29702254U DE29702254U1 (de) | 1996-07-12 | 1997-02-10 | Vorrichtung zur Aufreinigung von Molekülen |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2172801T3 true ES2172801T3 (es) | 2002-10-01 |
Family
ID=26027454
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES97931688T Expired - Lifetime ES2172801T3 (es) | 1996-07-12 | 1997-06-28 | Procedimiento y dispositivo para la purificacion y enriquecimiento de moleculas. |
Country Status (8)
Country | Link |
---|---|
US (1) | US6479644B1 (es) |
EP (1) | EP0912470B9 (es) |
JP (1) | JP2000515508A (es) |
AT (1) | ATE213227T1 (es) |
AU (1) | AU719533B2 (es) |
CA (1) | CA2260132A1 (es) |
ES (1) | ES2172801T3 (es) |
WO (1) | WO1998002399A1 (es) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU3931699A (en) * | 1998-05-04 | 1999-11-23 | Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V. | Method and device for isolating nucleic acids |
DE10006491A1 (de) | 1999-02-15 | 2000-08-24 | Fraunhofer Ges Forschung | Verfahren und Probenträgersystem zur Trennung und Anreicherung von Stoffen in situ |
DE19940647C2 (de) * | 1999-08-26 | 2002-11-28 | November Ag Molekulare Medizin | Verfahren zum Nachweis und zur Quantifizierung von in einer Flüssigkeit befindlichen ersten Biopolymeren |
DE10025174A1 (de) * | 2000-05-24 | 2001-12-06 | November Ag Molekulare Medizin | Verfahren zur Herstellung einer mit Biomolekülen beschichteten Elektrode |
DE10211741B4 (de) * | 2002-03-14 | 2005-12-01 | November Ag | Verfahren zum elektrochemischen Detektieren eines Analyten |
EP1734111B1 (en) | 2005-06-13 | 2020-06-24 | Tosoh Corporation | Cell fusion device, and method for cell fusion using the same |
CN101317086B (zh) * | 2005-10-04 | 2013-08-14 | 海德威技术公司 | 分析物的微观流体检测 |
JP2009273459A (ja) * | 2008-04-15 | 2009-11-26 | Tosoh Corp | 細胞選別装置、およびそれを用いた細胞選別方法 |
CN103900894A (zh) * | 2008-06-06 | 2014-07-02 | 华盛顿大学 | 用于从溶液浓缩颗粒的方法和系统 |
US8940092B1 (en) | 2008-10-27 | 2015-01-27 | University Of Washington Through Its Center For Commercialization | Hybrid fibers, devices using hybrid fibers, and methods for making hybrid fibers |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1152449A (en) | 1979-12-31 | 1983-08-23 | Patrick H. O'farrell | Method and apparatus for dynamic equilibrium electrophoresis |
US4545888A (en) * | 1984-04-06 | 1985-10-08 | Walsh J William | Apparatus for electrophoretic recovery of nucleic acids and other substances |
FR2574311B1 (fr) * | 1984-12-11 | 1989-09-15 | Inst Nat Sante Rech Med | Perfectionnements aux procedes et appareils d'electrophorese preparative |
JPS62184343A (ja) | 1986-02-07 | 1987-08-12 | Fuji Photo Film Co Ltd | 電気泳動装置 |
JPS63317756A (ja) | 1987-06-19 | 1988-12-26 | Fuji Photo Film Co Ltd | 電気泳動装置 |
GB8729002D0 (en) | 1987-12-11 | 1988-01-27 | Iq Bio Ltd | Electrode material |
AT390274B (de) * | 1988-03-15 | 1990-04-10 | Steininger Karl Heinz | Elektrode |
NL8801073A (nl) * | 1988-04-26 | 1989-11-16 | Univ Twente | Detectiemethode. |
US5196099A (en) | 1990-04-13 | 1993-03-23 | W. R. Grace & Co.-Conn. | Electrophoretic matrix and electrophoresis using same |
US5776672A (en) * | 1990-09-28 | 1998-07-07 | Kabushiki Kaisha Toshiba | Gene detection method |
US5217593A (en) * | 1991-03-13 | 1993-06-08 | Macconnell William P | Nucleic acid purification system and method |
US6017696A (en) * | 1993-11-01 | 2000-01-25 | Nanogen, Inc. | Methods for electronic stringency control for molecular biological analysis and diagnostics |
FR2687931A1 (fr) | 1992-02-28 | 1993-09-03 | Centre Nat Rech Scient | Procedes d'electrophorese et d'optimisation d'electrophorese et appareil pour leur mise en óoeuvre. |
JPH05236997A (ja) * | 1992-02-28 | 1993-09-17 | Hitachi Ltd | ポリヌクレオチド捕捉用チップ |
WO1994025144A1 (en) | 1993-05-05 | 1994-11-10 | Amrad Corporation Limited | Electrophoretic resolution of charged molecules |
US5871918A (en) * | 1996-06-20 | 1999-02-16 | The University Of North Carolina At Chapel Hill | Electrochemical detection of nucleic acid hybridization |
US5968745A (en) * | 1995-06-27 | 1999-10-19 | The University Of North Carolina At Chapel Hill | Polymer-electrodes for detecting nucleic acid hybridization and method of use thereof |
-
1997
- 1997-06-28 AU AU35376/97A patent/AU719533B2/en not_active Ceased
- 1997-06-28 JP JP10505499A patent/JP2000515508A/ja not_active Withdrawn
- 1997-06-28 ES ES97931688T patent/ES2172801T3/es not_active Expired - Lifetime
- 1997-06-28 US US09/214,629 patent/US6479644B1/en not_active Expired - Fee Related
- 1997-06-28 CA CA002260132A patent/CA2260132A1/en not_active Abandoned
- 1997-06-28 AT AT97931688T patent/ATE213227T1/de not_active IP Right Cessation
- 1997-06-28 EP EP97931688A patent/EP0912470B9/de not_active Expired - Lifetime
- 1997-06-28 WO PCT/DE1997/001368 patent/WO1998002399A1/de active IP Right Grant
Also Published As
Publication number | Publication date |
---|---|
EP0912470A1 (de) | 1999-05-06 |
AU719533B2 (en) | 2000-05-11 |
EP0912470B9 (de) | 2002-09-25 |
EP0912470B1 (de) | 2002-02-13 |
WO1998002399A1 (de) | 1998-01-22 |
US6479644B1 (en) | 2002-11-12 |
ATE213227T1 (de) | 2002-02-15 |
CA2260132A1 (en) | 1998-01-22 |
JP2000515508A (ja) | 2000-11-21 |
AU3537697A (en) | 1998-02-09 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ATE469911T1 (de) | Isolierte nukleinsäuremoleküle die t-zellen induzierbare faktoren kodieren (tif), kodierte proteine und ihre anwendung | |
TR200100056T2 (tr) | Nörotrofik faktörler | |
DE60144145D1 (de) | Subtilisin-variante | |
ES2153037T3 (es) | Lacasas purificadas de scytalidium y acidos nucleicos que las codifican. | |
WO1996034107A3 (en) | Antiviral proteins, dna coding sequences therefor, and uses thereof | |
DK1062226T3 (da) | Nukleinsyreligander med høj affinitet mod komplementsystemets C5-protein | |
DE69838147D1 (de) | Verbindungen und methoden zur modulierung von occludin-abhängiger gewebepermeabilität | |
DE60139262D1 (de) | System zur wiederherstellung von gelenkoberflächen von säugetieren | |
DE60136490D1 (de) | Sn-38-lipidkomplexe und verfahren zu ihrer verwendung | |
ID21045A (id) | Asam amino termodifikasi, obat-obatan yang mengandung senyawa ini dan proses pembuatannya | |
ES2172801T3 (es) | Procedimiento y dispositivo para la purificacion y enriquecimiento de moleculas. | |
KR890000517A (ko) | 하이브리드 단백질 c 및 이의 제조방법 | |
DE69840150D1 (de) | Verfahren, basierend auf affinitätskreuzbindung, der interaktion von biologisch aktiven peptiden an antikörper | |
TR199802394A3 (tr) | Proteinleri stabilize etmek için gelistirilmis proses. | |
BR0312521A (pt) | Métodos para a separação e a purificação de fibrinogênio e de pelo menos uma outra proteìna e para a co-purificação de fibrinogênio e de fator xiii, uso de cromatografia de afinidade com ìon de metal imobilizado, fibrinogênio, kit farmacêutico, e, formulações farmacêutica e de fibrinogênio liofilizado | |
IT1291623B1 (it) | Procedimento per la coniugazione di chelanti con molecole contenenti gruppi amminici | |
EA200000196A1 (ru) | Универсальная плазма крови | |
WO1996024650A3 (en) | Cytoplasmic antiproteinase-2 and cytoplasmic antiproteinase-3 and coding sequences | |
WO2002010393A3 (en) | Isolated nucleic acid molecules which encode t cell inducible factors, or interleukin-21, the proteins encoded, and uses thereof | |
DE60139544D1 (de) | Veränderte, fluoreszierende proteine | |
ATE309356T1 (de) | Konstruierte fluoreszenzproteine mit langen wellenlängen | |
ATE196926T1 (de) | Gen grb3-3, variante und ihre verwendungen | |
DE69734586D1 (de) | Retinoid-metabolisierendes protein | |
AU3042497A (en) | Vaccine containing a peroxiredoxin and/or a beta-tubulin | |
DK0643719T3 (da) | Nukleinsyresekvenser, som koder for eller er komplementære til nukleinsyresekvenser, som koder for interleukin 9-receptor |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
Ref document number: 912470 Country of ref document: ES |