ES2166371T3 - Celulas de levadura tratadas para producir sustitutos de proteinas de sistemas de feromonas y usos de las mismas. - Google Patents

Celulas de levadura tratadas para producir sustitutos de proteinas de sistemas de feromonas y usos de las mismas.

Info

Publication number
ES2166371T3
ES2166371T3 ES94912292T ES94912292T ES2166371T3 ES 2166371 T3 ES2166371 T3 ES 2166371T3 ES 94912292 T ES94912292 T ES 94912292T ES 94912292 T ES94912292 T ES 94912292T ES 2166371 T3 ES2166371 T3 ES 2166371T3
Authority
ES
Spain
Prior art keywords
yeast cells
proteins
same
pheromone
surrogate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94912292T
Other languages
English (en)
Inventor
Dana Fowlkes
Jim Broach
John Manfredi
Christine Klein
Andrew J Murphy
Jeremy Paul
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cadus Pharmaceutical Corp
Original Assignee
Cadus Pharmaceutical Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cadus Pharmaceutical Corp filed Critical Cadus Pharmaceutical Corp
Application granted granted Critical
Publication of ES2166371T3 publication Critical patent/ES2166371T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Cash Registers Or Receiving Machines (AREA)
  • Inspection Of Paper Currency And Valuable Securities (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

CELULAS DE LEVADURAS EN LAS QUE SE HAN APLICADO TECNICAS DE INGENIERIA GENETICA PARA EXPRESAR TANTO UN SUSTITUTO DE UNA PROTEINA DE UN SISTEMA DE FEROMONAS (EJ., ENZIMAS QUE INTERVIENEN EN LA MADURACION DEL {AL}-FACTOR, TRANSPORTADORES DEL {AL}FACTOR, RECEPTORES DE FEROMONAS, ETC.) Y UN MODULADOR DE PEPTIDOS POTENCIAL DEL SUSTITUTO, DE TAL MODO QUE LA INHIBICION O ACTIVACION DEL SUSTITUTO AFECTA A LAS CARACTERISTICAS SELECCIONABLES O SEPARABLES DE LAS CELULAS DE LEVADURAS. VARIAS CARACTERISTICAS ADICIONALES MEJORAN LA RELACION DE SEÑAL A RUIDO DEL SISTEMA DE SEPARACION/SELECCION.
ES94912292T 1993-03-31 1994-03-23 Celulas de levadura tratadas para producir sustitutos de proteinas de sistemas de feromonas y usos de las mismas. Expired - Lifetime ES2166371T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4143193A 1993-03-31 1993-03-31
US19032894A 1994-01-31 1994-01-31

Publications (1)

Publication Number Publication Date
ES2166371T3 true ES2166371T3 (es) 2002-04-16

Family

ID=26718132

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94912292T Expired - Lifetime ES2166371T3 (es) 1993-03-31 1994-03-23 Celulas de levadura tratadas para producir sustitutos de proteinas de sistemas de feromonas y usos de las mismas.

Country Status (10)

Country Link
EP (2) EP0915154A1 (es)
JP (3) JPH08510115A (es)
AT (1) ATE207118T1 (es)
AU (2) AU685103B2 (es)
CA (1) CA2158274C (es)
DE (1) DE69428689T2 (es)
DK (1) DK0692025T3 (es)
ES (1) ES2166371T3 (es)
PT (1) PT692025E (es)
WO (1) WO1994023025A1 (es)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5876951A (en) * 1993-03-31 1999-03-02 Cadus Pharmaceutical Corporation Yeast cells engineered to produce pheromone system protein surrogates and uses therefor
US6255059B1 (en) 1993-03-31 2001-07-03 Cadus Pharmaceutical Corporation Methods for identifying G protein coupled receptor effectors
US6100042A (en) 1993-03-31 2000-08-08 Cadus Pharmaceutical Corporation Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor
US20030054402A1 (en) 1993-03-31 2003-03-20 Cadus Pharmaceutical Corporation Methods and compositions for identifying receptor effectors
US5789184A (en) * 1993-03-31 1998-08-04 Cadus Pharmaceutical Corporation Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor
US7235648B1 (en) 1993-03-31 2007-06-26 Cadus Technologies, Inc. Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor
US7416881B1 (en) 1993-03-31 2008-08-26 Cadus Technologies, Inc. Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor
US6864060B1 (en) 1993-03-31 2005-03-08 Cadus Technologies, Inc. Yeast cells expressing modified G proteins and methods of use therefor
US5691188A (en) * 1994-02-14 1997-11-25 American Cyanamid Company Transformed yeast cells expressing heterologous G-protein coupled receptor
CA2188950C (en) * 1994-04-26 2001-07-03 James R. Broach Functional expression of mammalian adenylyl cyclase in yeast
AU723619B2 (en) * 1995-04-24 2000-08-31 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
CA2220459A1 (en) * 1995-06-07 1996-12-19 Cadus Pharmaceutical Corporation Expression of functional vertebrate phospholipases in yeast
CA2232733A1 (en) * 1995-09-20 1997-03-27 Orikkalapat Prem Das Yeast receptor and g-protein fusion protein
WO1997040170A2 (en) * 1996-04-23 1997-10-30 Cadus Pharmaceutical Corporation Functional expression of heterologous adenylyl cyclase
US6242211B1 (en) 1996-04-24 2001-06-05 Terragen Discovery, Inc. Methods for generating and screening novel metabolic pathways
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
DE69715621T2 (de) * 1996-04-26 2003-05-22 Massachusetts Inst Technology Drei hybriden screening test
CA2266543C (en) * 1996-09-24 2003-12-09 Cadus Pharmaceutical Corporation Methods and compositions for identifying receptor effectors
CA2293270A1 (en) 1997-06-11 1998-12-17 New York University Prenylcysteine carboxyl methyltransferase, dna encoding same, and a method of screening for inhibitors thereof
EP1021538A1 (en) * 1997-10-07 2000-07-26 Cadus Pharmaceutical Corporation Yeast cells expressing modified g proteins and methods of use therefor
US6355473B1 (en) 1998-05-06 2002-03-12 Cadus Pharmaceutical Corp. Yeast cells having mutations in stp22 and uses therefor
US6733991B1 (en) 1998-05-08 2004-05-11 Osi Pharmaceuticals, Inc. AGS proteins and nucleic acid molecules and uses therefor
AU3977699A (en) * 1998-05-08 1999-11-29 Cadus Pharmaceutical Corporation Ags proteins and nucleic acid molecules and uses therefor
US6746852B1 (en) 1998-05-08 2004-06-08 Osi Pharmaceuticals, Inc. AGS proteins and nucleic acid molecules and uses thereof
US20020015967A1 (en) * 1998-06-02 2002-02-07 Lauren Silverman Functional expression of adenosine receptors in yeast
US7081360B2 (en) 1998-07-28 2006-07-25 Cadus Technologies, Inc. Expression of G protein-coupled receptors with altered ligand binding and/or coupling properties
US7273747B2 (en) 1998-08-27 2007-09-25 Cadus Technologies, Inc. Cell having amplified signal transduction pathway responses and uses therefor
US6251605B1 (en) 1998-10-27 2001-06-26 Cadus Pharmaceutical Corporation Yeast cells having mutations in Cav1 and uses therefor
AU2030000A (en) 1998-11-25 2000-06-13 Cadus Pharmaceutical Corporation Methods and compositions for identifying receptor effectors
US6504008B1 (en) 1999-02-01 2003-01-07 Cadus Technologies, Inc. Cell based signal generation
US6555325B1 (en) 1999-06-14 2003-04-29 Cadus Technologies, Inc. System for detection of a functional interaction between a compound and a cellular signal transduction component
US7223533B2 (en) 1999-09-10 2007-05-29 Cadus Technologies, Inc. Cell surface proteins and use thereof as indicators of activation of cellular signal transduction pathways
GB0030038D0 (en) * 2000-12-08 2001-01-24 Univ Warwick Yeast-based assay
EP1975620A3 (en) 2001-03-02 2008-12-24 GPC Biotech AG Three hybrid assay system
NZ513755A (en) * 2001-08-24 2001-09-28 Ann Rachel Holmes Protein expression system in yeast comprising a vector encoding a heterologous membrane protein and its application in screening for drugs
AU2013212008A1 (en) * 2012-01-25 2014-07-10 Alfa Wassermann S.P.A. Rifaximin derivative and uses thereof
BR112021003545A2 (pt) * 2018-08-29 2021-05-18 Amyris, Inc. células e métodos para ensaio baseado em seleção
CN109593773B (zh) * 2018-11-22 2021-07-30 北京利德曼生化股份有限公司 一种应用酵母表达系统表达可溶性生长刺激表达基因2蛋白的方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988010308A1 (en) * 1987-06-24 1988-12-29 Whitehead Institute For Biomedical Research Pheromone-inducible yeast promoter
US5284746A (en) * 1990-02-08 1994-02-08 Zymogenetics, Inc. Methods of producing hybrid G protein-coupled receptors
DK0548165T3 (da) * 1990-09-13 2001-07-16 Univ Duke Ekspression af G-proteinkoblede receptorer i gær

Also Published As

Publication number Publication date
AU685103B2 (en) 1998-01-15
DK0692025T3 (da) 2002-02-11
CA2158274A1 (en) 1994-10-13
AU6354198A (en) 1998-07-09
AU6490994A (en) 1994-10-24
WO1994023025A1 (en) 1994-10-13
EP0692025B1 (en) 2001-10-17
DE69428689T2 (de) 2002-08-08
JP2004337174A (ja) 2004-12-02
JPH08510115A (ja) 1996-10-29
PT692025E (pt) 2002-04-29
DE69428689D1 (de) 2001-11-22
EP0692025A1 (en) 1996-01-17
CA2158274C (en) 2000-05-30
JP2004357713A (ja) 2004-12-24
EP0915154A1 (en) 1999-05-12
ATE207118T1 (de) 2001-11-15

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