ES2162044T3 - Procedimiento para la reduccion de la formacion de artefactos durante la transcripcion de acidos ribonucleicos en acidos desoxirribonucleicos. - Google Patents
Procedimiento para la reduccion de la formacion de artefactos durante la transcripcion de acidos ribonucleicos en acidos desoxirribonucleicos.Info
- Publication number
- ES2162044T3 ES2162044T3 ES96910034T ES96910034T ES2162044T3 ES 2162044 T3 ES2162044 T3 ES 2162044T3 ES 96910034 T ES96910034 T ES 96910034T ES 96910034 T ES96910034 T ES 96910034T ES 2162044 T3 ES2162044 T3 ES 2162044T3
- Authority
- ES
- Spain
- Prior art keywords
- acids
- procedure
- transcription
- deoxirribonucleic
- reduction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pyrane Compounds (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Lighting Device Outwards From Vehicle And Optical Signal (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
Abstract
LA INVENCION TRATA DE UN PROCEDIMIENTO PARA LA TRANSCRIPCION DE LOS ACIDOS RIBONUCLEICOS DE UNA MUESTRA TRATANDO LA MUESTRA QUE CONTIENE EL ACIDO RIBONUCLEICO CON UNA ENZIMA QUE DESCOMPONE EL ADN EN PRESENCIA DE IONES MN 2+ Y LA POSTERIOR TRANSCRIPCION DE LOS ACIDOS RIBONUCLEICOS EN ACIDOS DESOXIRIBONUCLEICOS. LA INVENCION TRATA TAMBIEN DE UNOS REACTIVOS ADECUADOS PARA LA REALIZACION DEL PROCEDIMIENTO.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19513728A DE19513728A1 (de) | 1995-04-11 | 1995-04-11 | Verfahren zur Reduzierung der Bildung von Artefakten |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2162044T3 true ES2162044T3 (es) | 2001-12-16 |
Family
ID=7759479
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES96910034T Expired - Lifetime ES2162044T3 (es) | 1995-04-11 | 1996-04-10 | Procedimiento para la reduccion de la formacion de artefactos durante la transcripcion de acidos ribonucleicos en acidos desoxirribonucleicos. |
Country Status (10)
Country | Link |
---|---|
US (1) | US6124093A (es) |
EP (1) | EP0871769B1 (es) |
JP (1) | JP4126348B2 (es) |
AT (1) | ATE205544T1 (es) |
AU (1) | AU5335596A (es) |
DE (2) | DE19513728A1 (es) |
ES (1) | ES2162044T3 (es) |
IL (1) | IL117880A0 (es) |
WO (1) | WO1996032501A1 (es) |
ZA (1) | ZA962836B (es) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9716664D0 (en) | 1997-08-06 | 1997-10-15 | Norwegian Inst Of Fisheries & | A method of removing nucleic acid contamination reactions |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5561058A (en) * | 1986-08-22 | 1996-10-01 | Hoffmann-La Roche Inc. | Methods for coupled high temperatures reverse transcription and polymerase chain reactions |
US5798257A (en) * | 1990-07-09 | 1998-08-25 | Research Corporation Technologies, Inc. | Nucleic acid encoding human MTS-1 protein |
FR2690691B1 (fr) * | 1992-04-29 | 1999-02-12 | Bio Merieux | Procede d'amplification d'arn necessitant une seule etape de manipulation. |
WO1994012657A1 (en) * | 1992-11-20 | 1994-06-09 | Katcher Harold L | Direct-phenol pcr, rt and rt-pcr methods |
ATE205259T1 (de) * | 1993-07-01 | 2001-09-15 | Hoffmann La Roche | Reagenz und verfahren für reverse transkription bei hoher temperatur, verbunden mit einer polymerase-kettenreaktion |
-
1995
- 1995-04-11 DE DE19513728A patent/DE19513728A1/de not_active Ceased
-
1996
- 1996-04-10 DE DE59607694T patent/DE59607694D1/de not_active Expired - Lifetime
- 1996-04-10 AU AU53355/96A patent/AU5335596A/en not_active Abandoned
- 1996-04-10 ES ES96910034T patent/ES2162044T3/es not_active Expired - Lifetime
- 1996-04-10 AT AT96910034T patent/ATE205544T1/de not_active IP Right Cessation
- 1996-04-10 JP JP53070396A patent/JP4126348B2/ja not_active Expired - Lifetime
- 1996-04-10 EP EP96910034A patent/EP0871769B1/de not_active Expired - Lifetime
- 1996-04-10 ZA ZA9602836A patent/ZA962836B/xx unknown
- 1996-04-10 WO PCT/EP1996/001524 patent/WO1996032501A1/de active IP Right Grant
- 1996-04-10 US US08/930,601 patent/US6124093A/en not_active Expired - Lifetime
- 1996-04-11 IL IL11788096A patent/IL117880A0/xx unknown
Also Published As
Publication number | Publication date |
---|---|
EP0871769A1 (de) | 1998-10-21 |
ZA962836B (en) | 1997-10-10 |
DE19513728A1 (de) | 1996-10-31 |
EP0871769B1 (de) | 2001-09-12 |
US6124093A (en) | 2000-09-26 |
DE59607694D1 (de) | 2001-10-18 |
JP4126348B2 (ja) | 2008-07-30 |
AU5335596A (en) | 1996-10-30 |
ATE205544T1 (de) | 2001-09-15 |
WO1996032501A1 (de) | 1996-10-17 |
JPH11503325A (ja) | 1999-03-26 |
IL117880A0 (en) | 1996-12-05 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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