ES2151901T3 - Metodo y aparato para propagar particulas de biomasa. - Google Patents
Metodo y aparato para propagar particulas de biomasa.Info
- Publication number
- ES2151901T3 ES2151901T3 ES93903440T ES93903440T ES2151901T3 ES 2151901 T3 ES2151901 T3 ES 2151901T3 ES 93903440 T ES93903440 T ES 93903440T ES 93903440 T ES93903440 T ES 93903440T ES 2151901 T3 ES2151901 T3 ES 2151901T3
- Authority
- ES
- Spain
- Prior art keywords
- culture medium
- settling chamber
- vessel
- particle
- agitated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/22—Settling tanks; Sedimentation by gravity
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
LA PRESENTE INVENCION SE REFIERE A UN METODO Y APARATO MEJORADOS DE CRECIMIENTO DE PARTICULAS DE BIOMASA (15), PARTICULARMENTE CELULAS ENLAZADAS DE MICROPORTADORAS, EN UN RECIPIENTE DE CULTIVO EN SUSPENSION (10) EN EL CUAL SE AÑADE UN MEDIO DE CULTIVO FRESCO Y EL MEDIO DE CULTIVO GASTADO SE RETIRA CONTINUA O SEMICONTINUAMENTE. LA MEJORA CONSISTE EN RETIRAR EL MEDIO DE CULTIVO GASTADO A TRAVES DE UNA CAMARA DE ASENTAMIENTO DE PARTICULAS (26) SITUADA DENTRO DEL RECIPIENTE (10) Y AL MENOS PARCIALMENTE INMERSO EN EL MEDIO DE CULTIVO AGITADO EN SU INTERIOR. LA CAMARA DE ASENTAMIENTO DE PARTICULAS (26) COMPRENDE UN CONTENEDOR HUECO (27, 28) CON UNA ABERTURA INFERIOR (18) A TRAVES DE LA CUAL LAS PARTICULAS DE BIOMASA (15),TAL COMO CELULAS ENLAZADAS DE MICROPORTADORAS, SE ESTABLECEN POR UN REFUERZO DE GRAVEDAD DENTRO DEL MEDIO DE CULTIVO AGITADO Y UNA ABERTURA SUPERIOR (19) A TRAVES DE LA CUAL SE RETIRA EL MEDIO DE CULTIVO GASTADO LIBRE DE PARTICULAS DEL RECIPIENTE (10). LA CAMARA DE ASENTAMIENTO (26) ESTA CONFIGURADA DE MANERA QUE LA VELOCIDAD DE FLUIDO DE MEDIO DE CULTIVO SE INTRODUCE DENTRO DE LA CAMARA DE ASENTAMIENTO (26) A TRAVES DE LA ABERTURA INFERIOR (18) ES SIGNIFICANTEMENTE INFERIOR A LA VELOCIDAD DE ASENTAMIENTO DE PARTICULAS DE BIOMASA.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US82230192A | 1992-01-17 | 1992-01-17 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2151901T3 true ES2151901T3 (es) | 2001-01-16 |
Family
ID=25235685
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES93903440T Expired - Lifetime ES2151901T3 (es) | 1992-01-17 | 1993-01-15 | Metodo y aparato para propagar particulas de biomasa. |
Country Status (15)
Country | Link |
---|---|
US (1) | US5654197A (es) |
EP (1) | EP0585419B1 (es) |
JP (1) | JP3244701B2 (es) |
AT (1) | ATE197813T1 (es) |
AU (1) | AU664596B2 (es) |
CA (1) | CA2106064C (es) |
DE (1) | DE69329706T2 (es) |
DK (1) | DK0585419T3 (es) |
ES (1) | ES2151901T3 (es) |
GR (1) | GR3035408T3 (es) |
HK (1) | HK1012674A1 (es) |
IL (1) | IL104385A (es) |
PT (1) | PT585419E (es) |
WO (1) | WO1993014192A1 (es) |
ZA (1) | ZA93310B (es) |
Families Citing this family (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5998184A (en) * | 1997-10-08 | 1999-12-07 | Unisyn Technologies, Inc. | Basket-type bioreactor |
US6335191B1 (en) | 1998-02-27 | 2002-01-01 | Nch Corporation | Automated system and method for growing bacteria |
DE19918409A1 (de) * | 1999-04-22 | 2000-10-26 | Forschungszentrum Juelich Gmbh | Bioreaktor umfassend einen Medienablauf und einen Rührer sowie Rührer und Medienablauf |
US20040185534A1 (en) * | 2000-10-02 | 2004-09-23 | Knudsen Ida Molgaard | Industrial-scale serum-free production of recombinant proteins in mammalian cells |
DE60137950D1 (de) * | 2000-10-02 | 2009-04-23 | Novo Nordisk Healthcare Ag | Verfahren zur herstellung vitamin-k-abhängiger proteine |
US20040185535A1 (en) * | 2003-03-21 | 2004-09-23 | Giles Wilson | Industrial-scale serum-free production of recombinant FVII in mammalian cells |
US20020179544A1 (en) * | 2001-04-27 | 2002-12-05 | Nexell Therapeutics, Inc. | Cell processing and fluid transfer apparatus and method of use |
PL368119A1 (en) * | 2001-10-02 | 2005-03-21 | Novo Nordisk Health Care Ag | Method for production of recombinant proteins in eukaryote cells |
US7160719B2 (en) | 2002-06-07 | 2007-01-09 | Mayo Foundation For Medical Education And Research | Bioartificial liver system |
CN1300297C (zh) * | 2002-09-20 | 2007-02-14 | 华东理工大学 | 一种用于动物细胞扩大接种的消化反应器 |
US7081361B2 (en) * | 2003-08-07 | 2006-07-25 | Nch Corporation | Biomass generator |
US20080020049A1 (en) * | 2005-02-25 | 2008-01-24 | Andrew Darling | Super-sparger microcarrier beads and precision extrusion deposited poly-epsilon-caprolactone structures for biological applications |
CN102421467B (zh) | 2009-03-13 | 2015-04-22 | 梅约医学教育与研究基金会 | 生物人工肝 |
US8551762B2 (en) | 2009-07-07 | 2013-10-08 | Nch Corporation | System and apparatus for feeding, solubilizing, growing and discharging a biological material |
US8961893B2 (en) * | 2009-07-07 | 2015-02-24 | Nch Corporation | Automated chemical diluter system having disposable components |
DE102009026366A1 (de) | 2009-08-12 | 2011-02-17 | Technische Universität München | Fermentationsbehälter sowie Verfahren und Vorrichtung zur Fermentation |
JP5415330B2 (ja) * | 2010-03-12 | 2014-02-12 | 株式会社日立製作所 | 生体細胞の培養装置及び培養方法 |
EP2665806B1 (en) * | 2011-01-17 | 2021-07-28 | F. Hoffmann-La Roche AG | Separation apparatus |
US20170096628A1 (en) * | 2014-04-14 | 2017-04-06 | Enevor Inc. | Conical Impeller and Applications Thereof |
US20170218330A1 (en) * | 2016-02-01 | 2017-08-03 | Fenwal, Inc. | Systems and methods for the separation of cells from microcarriers using a spinning membrane |
JP6744161B2 (ja) * | 2016-07-19 | 2020-08-19 | 株式会社日立プラントサービス | 分離装置、培養装置および分離方法 |
CN114058505A (zh) | 2020-07-30 | 2022-02-18 | 环球生命科技咨询美国有限责任公司 | 新型灌流培养用高密度微载体截留装置和使用方法 |
JP2023003462A (ja) * | 2021-06-24 | 2023-01-17 | 横河電機株式会社 | 細胞培養プロセスに使用するための溶液処理デバイス |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR821326A (fr) * | 1936-05-04 | 1937-12-02 | Procédé et appareil pour la fermentation continue de solutions, en vue de l'obtention de produits de fermentation ou de micro-organismes qui se multiplient | |
US2451156A (en) * | 1944-06-19 | 1948-10-12 | Mattos Annibal Ramos De | Process and apparatus for producing alcohol by fermentation |
JPS575690A (en) * | 1980-06-13 | 1982-01-12 | Toray Ind Inc | Circulation device for culture medium in cultivation tank |
US4335215A (en) * | 1980-08-27 | 1982-06-15 | Monsanto Company | Method of growing anchorage-dependent cells |
CS231837B1 (en) * | 1982-07-30 | 1984-12-14 | Svatopluk Mackrle | Device for a biological water purification |
US4814278A (en) * | 1985-02-05 | 1989-03-21 | Teijin Limited | Culture apparatus and method |
JPS63123379A (ja) * | 1986-11-12 | 1988-05-27 | Teijin Ltd | 動物細胞の培養方法 |
GB8515636D0 (en) * | 1985-06-20 | 1985-07-24 | Celltech Ltd | Fermenter |
JPH0195769A (ja) * | 1987-10-08 | 1989-04-13 | Ebara Res Co Ltd | 高濃度培養装置 |
JPH0380077A (ja) * | 1989-08-23 | 1991-04-04 | Meiji Milk Prod Co Ltd | マイクロキャリア分離方法及び装置 |
EP0498858A1 (en) * | 1989-11-03 | 1992-08-19 | Massachusetts Institute Of Technology | Nested-cone separator |
JP3385741B2 (ja) * | 1994-08-22 | 2003-03-10 | 日本ゼオン株式会社 | 医用材料組成物、その調製方法及び医用成形物 |
-
1993
- 1993-01-13 IL IL10438593A patent/IL104385A/en not_active IP Right Cessation
- 1993-01-15 CA CA002106064A patent/CA2106064C/en not_active Expired - Fee Related
- 1993-01-15 JP JP51262993A patent/JP3244701B2/ja not_active Expired - Fee Related
- 1993-01-15 DE DE69329706T patent/DE69329706T2/de not_active Expired - Lifetime
- 1993-01-15 PT PT93903440T patent/PT585419E/pt unknown
- 1993-01-15 WO PCT/US1993/000272 patent/WO1993014192A1/en active IP Right Grant
- 1993-01-15 ES ES93903440T patent/ES2151901T3/es not_active Expired - Lifetime
- 1993-01-15 EP EP93903440A patent/EP0585419B1/en not_active Expired - Lifetime
- 1993-01-15 AT AT93903440T patent/ATE197813T1/de active
- 1993-01-15 AU AU34703/93A patent/AU664596B2/en not_active Ceased
- 1993-01-15 DK DK93903440T patent/DK0585419T3/da active
- 1993-01-18 ZA ZA93310A patent/ZA93310B/xx unknown
- 1993-08-06 US US08/102,098 patent/US5654197A/en not_active Expired - Lifetime
-
1998
- 1998-12-17 HK HK98113868A patent/HK1012674A1/xx not_active IP Right Cessation
-
2001
- 2001-02-14 GR GR20010400237T patent/GR3035408T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
CA2106064A1 (en) | 1993-07-18 |
DE69329706D1 (de) | 2001-01-04 |
JP3244701B2 (ja) | 2002-01-07 |
EP0585419A4 (en) | 1995-04-05 |
ZA93310B (en) | 1993-09-08 |
DE69329706T2 (de) | 2001-03-29 |
US5654197A (en) | 1997-08-05 |
CA2106064C (en) | 2005-09-20 |
EP0585419B1 (en) | 2000-11-29 |
IL104385A0 (en) | 1993-05-13 |
GR3035408T3 (en) | 2001-05-31 |
EP0585419A1 (en) | 1994-03-09 |
AU664596B2 (en) | 1995-11-23 |
JPH06510205A (ja) | 1994-11-17 |
WO1993014192A1 (en) | 1993-07-22 |
DK0585419T3 (da) | 2001-03-19 |
HK1012674A1 (en) | 1999-08-06 |
PT585419E (pt) | 2001-04-30 |
AU3470393A (en) | 1993-08-03 |
ATE197813T1 (de) | 2000-12-15 |
IL104385A (en) | 1995-12-31 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ES2151901T3 (es) | Metodo y aparato para propagar particulas de biomasa. | |
JP3583785B2 (ja) | 微生物生育工程の制御方法 | |
EP0699101B1 (en) | Particle settler for use in mammalian cell culture | |
DE3661273D1 (en) | Method and apparatus for carrying out cell culture | |
ES2095944T3 (es) | Conglomerado portador de sustancias. | |
ATE306984T1 (de) | Vorrichtung und verfahren zum rühren von teilchen in suspension in einem flüssigen medium | |
DE3165197D1 (en) | Method and apparatus for stirring particles in suspension such as microcarriers for anchorage-dependent living cells in a liquid culture medium | |
BR8100543A (pt) | Instalacao para ajustar a quantidade de levedura a ser introduzida em um meio de fermentacao | |
DE69617081D1 (de) | Vorrichtung zur biologischen Reinigung von Flüssigkeiten, insbesondere von Abwasser | |
Cherry et al. | Studies and observations on the growth of mammalian cells in agitated fluid medium | |
JPH0416153B2 (es) | ||
GB2054397B (en) | Method and apparatus for stirring particles in suspension such as microcarriers for anchorage-dependent living cells in a liquid culture medium | |
GB2017752A (en) | Apparatus for cultivating microorganisms | |
JPS645486A (en) | Cell culture method and apparatus therefor | |
DE3873511D1 (de) | Abwasserbehandlungsanlage. | |
JPS6486869A (en) | Method for rotating cultivation of plant cell and apparatus therefor | |
AU564836B2 (en) | Separating different constituents according to their specific gravity | |
CA1313149C (en) | Method of cell fusions induced by electric pulsations | |
Mooers | Studies on immobilization and product recovery for the magnetically stabilized fluidized bed plant cell bioreactor | |
CN117757729A (zh) | 一种诱导脐带间充质干细胞分化的方法 | |
EP0276154A3 (en) | Method and apparatus for cell culture | |
Badger et al. | A Method for the Selection of a Synchronized Population of Cloning HeLa Cells | |
JPS57140697A (en) | Aeration in active sludge process | |
Johnson | [61] Measurement of interferon modulation of the in vitro antibody response | |
JPS63123379A (ja) | 動物細胞の培養方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
Ref document number: 585419 Country of ref document: ES |