ES2145702A1 - Procedimiento enzimatico de obtencion de (r)-2-hidroxi-4-fenilbutanoato de etilo enantiomericamente puro utilizando biocatalizadores de lipasas inmovilizadas. - Google Patents

Procedimiento enzimatico de obtencion de (r)-2-hidroxi-4-fenilbutanoato de etilo enantiomericamente puro utilizando biocatalizadores de lipasas inmovilizadas.

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Publication number
ES2145702A1
ES2145702A1 ES009800708A ES9800708A ES2145702A1 ES 2145702 A1 ES2145702 A1 ES 2145702A1 ES 009800708 A ES009800708 A ES 009800708A ES 9800708 A ES9800708 A ES 9800708A ES 2145702 A1 ES2145702 A1 ES 2145702A1
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Spain
Prior art keywords
hydroxy
phenylbutanoate
immobilized
formula
enantiomerically pure
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Application number
ES009800708A
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English (en)
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ES2145702B1 (es
Inventor
Seijas Jose Manuel Guisan
Gil Pilar Armisen
Castrillo Pilar Sabuquillo
Lorente Gloria Fernandez
Lafuente Roberto Fernandez
Codina Agatha Bastida
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Vita Invest SA
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Vita Invest SA
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Publication date
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Priority to ES9800708A priority Critical patent/ES2145702B1/es
Publication of ES2145702A1 publication Critical patent/ES2145702A1/es
Application granted granted Critical
Publication of ES2145702B1 publication Critical patent/ES2145702B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

Procedimiento enzimáico de obtención de (r)-2-hidroxi-4-fenilbutanoato de etilo enantioméricamente puro utilizando biocatalizadores de lipasas inmovilizadas.Consiste en hidrolizar enantioselectivamente con lipasas inmovilizadas, la mezcla racémica (R, S)-2-hidroxi-4-fenilbutanoato de etilo ((R,S)-HPBE) de fórmula (I), obteniéndose (R)-2-hidroxi-4-fenilbutanoato de etilo ((R)-HPBE) de fórmula (II) y ácido (S)-2-hidroxi-4-feniltutanoico de fórmula (III).El compuesto de fórmula (II) es un importante intermedio enla síntesis de inhibidores de la enzima convertidora de laangiotensina. El procedimiento se lleva a cabo en medios acuosos, en condiciones suaves de pH y temperatura y utiliza biocatalizadores de lipasas inmovilizados obtenidos por adsorción de las enzimas, a muy baja fuerza iónica, en soportes hidrofílicos modificados químicamente con una capa densa de grupos hidrofóbicos bien definidos.
ES9800708A 1998-04-03 1998-04-03 Procedimiento enzimatico de obtencion de (r)-2-hidroxi-4-fenilbutanoato de etilo enantiomericamente puro utilizando biocatalizadores de lipasas inmovilizadas. Expired - Fee Related ES2145702B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9800708A ES2145702B1 (es) 1998-04-03 1998-04-03 Procedimiento enzimatico de obtencion de (r)-2-hidroxi-4-fenilbutanoato de etilo enantiomericamente puro utilizando biocatalizadores de lipasas inmovilizadas.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9800708A ES2145702B1 (es) 1998-04-03 1998-04-03 Procedimiento enzimatico de obtencion de (r)-2-hidroxi-4-fenilbutanoato de etilo enantiomericamente puro utilizando biocatalizadores de lipasas inmovilizadas.

Publications (2)

Publication Number Publication Date
ES2145702A1 true ES2145702A1 (es) 2000-07-01
ES2145702B1 ES2145702B1 (es) 2001-02-01

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ES9800708A Expired - Fee Related ES2145702B1 (es) 1998-04-03 1998-04-03 Procedimiento enzimatico de obtencion de (r)-2-hidroxi-4-fenilbutanoato de etilo enantiomericamente puro utilizando biocatalizadores de lipasas inmovilizadas.

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2161167A1 (es) * 1999-09-24 2001-11-16 Vita Invest Sa Procedimiento de hidrolisis enzimatica de esteres del acido (3sr,4rs)-4-(4-flurofenil)-6-oxopiperidin-3-carboxilico con biocatalizadores de lipasas o esterasas inmovilizadas.
WO2010139837A1 (es) * 2009-06-01 2010-12-09 Consejo Superior De Investigaciones Científicas (Csic) Complejos covalentes de lipasas con proteínas, sondas de adn, cofactores u otras biomoléculas

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4668628A (en) * 1985-04-01 1987-05-26 Stauffer Chemical Company Resolution of racemic mixtures of aliphatic acid esters
EP0325971A2 (de) * 1988-01-26 1989-08-02 F. Hoffmann-La Roche Ag Verfahren zur Herstellung optisch reiner Hydroxyarylalkansäuren und -ester

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4668628A (en) * 1985-04-01 1987-05-26 Stauffer Chemical Company Resolution of racemic mixtures of aliphatic acid esters
EP0325971A2 (de) * 1988-01-26 1989-08-02 F. Hoffmann-La Roche Ag Verfahren zur Herstellung optisch reiner Hydroxyarylalkansäuren und -ester

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
YAMANÉ, T. et al.: "Repeated use of lipase immobilized on amphiphilic gel for hydrolysis of a small amount of glycerides included in liquid crude fatty acid", (1982), J. Ferment. Technol., Vol. 60 (6), paginas 517-523 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2161167A1 (es) * 1999-09-24 2001-11-16 Vita Invest Sa Procedimiento de hidrolisis enzimatica de esteres del acido (3sr,4rs)-4-(4-flurofenil)-6-oxopiperidin-3-carboxilico con biocatalizadores de lipasas o esterasas inmovilizadas.
WO2010139837A1 (es) * 2009-06-01 2010-12-09 Consejo Superior De Investigaciones Científicas (Csic) Complejos covalentes de lipasas con proteínas, sondas de adn, cofactores u otras biomoléculas
ES2352779A1 (es) * 2009-06-01 2011-02-23 Consejo Superior De Investigaciones Cientificas (Csic) Complejos covalentes de lipasas con proteinas, sondas de adn, cofactores u otras biomoleculas.

Also Published As

Publication number Publication date
ES2145702B1 (es) 2001-02-01

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