ES2145702B1 - Procedimiento enzimatico de obtencion de (r)-2-hidroxi-4-fenilbutanoato de etilo enantiomericamente puro utilizando biocatalizadores de lipasas inmovilizadas. - Google Patents

Procedimiento enzimatico de obtencion de (r)-2-hidroxi-4-fenilbutanoato de etilo enantiomericamente puro utilizando biocatalizadores de lipasas inmovilizadas.

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Publication number
ES2145702B1
ES2145702B1 ES9800708A ES9800708A ES2145702B1 ES 2145702 B1 ES2145702 B1 ES 2145702B1 ES 9800708 A ES9800708 A ES 9800708A ES 9800708 A ES9800708 A ES 9800708A ES 2145702 B1 ES2145702 B1 ES 2145702B1
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Spain
Prior art keywords
hydroxy
formula
obtaining
immobilized
phenylbutanoate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES9800708A
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English (en)
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ES2145702A1 (es
Inventor
Seijas Jose Manuel Guisan
Gil Pilar Armisen
Castrillo Pilar Sabuquillo
Lorente Gloria Fernandez
Lafuente Roberto Fernandez
Codina Agatha Bastida
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Vita Invest SA
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Vita Invest SA
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Publication date
Application filed by Vita Invest SA filed Critical Vita Invest SA
Priority to ES9800708A priority Critical patent/ES2145702B1/es
Publication of ES2145702A1 publication Critical patent/ES2145702A1/es
Application granted granted Critical
Publication of ES2145702B1 publication Critical patent/ES2145702B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

Procedimiento enzimáico de obtención de (r)-2-hidroxi-4-fenilbutanoato de etilo enantioméricamente puro utilizando biocatalizadores de lipasas inmovilizadas.Consiste en hidrolizar enantioselectivamente con lipasas inmovilizadas, la mezcla racémica (R, S)-2-hidroxi-4-fenilbutanoato de etilo ((R,S)-HPBE) de fórmula (I), obteniéndose (R)-2-hidroxi-4-fenilbutanoato de etilo ((R)-HPBE) de fórmula (II) y ácido (S)-2-hidroxi-4-feniltutanoico de fórmula (III).El compuesto de fórmula (II) es un importante intermedio enla síntesis de inhibidores de la enzima convertidora de laangiotensina. El procedimiento se lleva a cabo en medios acuosos, en condiciones suaves de pH y temperatura y utiliza biocatalizadores de lipasas inmovilizados obtenidos por adsorción de las enzimas, a muy baja fuerza iónica, en soportes hidrofílicos modificados químicamente con una capa densa de grupos hidrofóbicos bien definidos.
ES9800708A 1998-04-03 1998-04-03 Procedimiento enzimatico de obtencion de (r)-2-hidroxi-4-fenilbutanoato de etilo enantiomericamente puro utilizando biocatalizadores de lipasas inmovilizadas. Expired - Fee Related ES2145702B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9800708A ES2145702B1 (es) 1998-04-03 1998-04-03 Procedimiento enzimatico de obtencion de (r)-2-hidroxi-4-fenilbutanoato de etilo enantiomericamente puro utilizando biocatalizadores de lipasas inmovilizadas.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9800708A ES2145702B1 (es) 1998-04-03 1998-04-03 Procedimiento enzimatico de obtencion de (r)-2-hidroxi-4-fenilbutanoato de etilo enantiomericamente puro utilizando biocatalizadores de lipasas inmovilizadas.

Publications (2)

Publication Number Publication Date
ES2145702A1 ES2145702A1 (es) 2000-07-01
ES2145702B1 true ES2145702B1 (es) 2001-02-01

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Family Applications (1)

Application Number Title Priority Date Filing Date
ES9800708A Expired - Fee Related ES2145702B1 (es) 1998-04-03 1998-04-03 Procedimiento enzimatico de obtencion de (r)-2-hidroxi-4-fenilbutanoato de etilo enantiomericamente puro utilizando biocatalizadores de lipasas inmovilizadas.

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ES (1) ES2145702B1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2161167B1 (es) * 1999-09-24 2002-07-01 Vita Invest Sa Procedimiento de hidrolisis enzimatica de esteres del acido (3sr,4rs)-4-(4-fluorofenil)-6-oxopiperidin-3-carboxilico con biocatalizadores de lipasas o esterasas inmovilizadas.
ES2352779B1 (es) * 2009-06-01 2012-01-26 Consejo Superior De Investigaciones Científicas (Csic) Complejos covalentes de lipasas con proteinas, sondas de adn, cofactores u otras biomoleculas

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4668628A (en) * 1985-04-01 1987-05-26 Stauffer Chemical Company Resolution of racemic mixtures of aliphatic acid esters
EP0325971A3 (de) * 1988-01-26 1990-07-25 F. Hoffmann-La Roche Ag Verfahren zur Herstellung optisch reiner Hydroxyarylalkansäuren und -ester

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