ES2143466T3 - Preparacion de anticuerpos quimericos usando la estrategia pcr recombinante. - Google Patents
Preparacion de anticuerpos quimericos usando la estrategia pcr recombinante.Info
- Publication number
- ES2143466T3 ES2143466T3 ES91919298T ES91919298T ES2143466T3 ES 2143466 T3 ES2143466 T3 ES 2143466T3 ES 91919298 T ES91919298 T ES 91919298T ES 91919298 T ES91919298 T ES 91919298T ES 2143466 T3 ES2143466 T3 ES 2143466T3
- Authority
- ES
- Spain
- Prior art keywords
- cdr
- primers
- antibody
- preparation
- chemical
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/46—Hybrid immunoglobulins
- C07K16/461—Igs containing Ig-regions, -domains or -residues form different species
- C07K16/464—Igs containing CDR-residues from one specie grafted between FR-residues from another
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/867—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof involving immunoglobulin or antibody produced via recombinant dna technology
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
LA INVENCION SE REFIERE A UN METODO PARA LA PRODUCCION DE UN ANTICUERPO QUIMERICO EN QUE EL CDR DE UN PRIMER ANTICUERPO SE UNE ENTRE LAS REGIONES DE MARCO DE UN SEGUNDO ANTICUERPO. EL METODO SE LLEVA A CABO UTILIZANDO UNA PLANTILLA QUE CONSTA DE DOS REGIONES DE MARCO, AB Y CD, Y ENTRE ELLAS, EL CDR QUE VA A SER SUSTITUIDO POR UN CDR DONADOR. SE UTILIZAN IMPRIMADORES A Y B PARA AMPLIFICAR LA REGION DE MARCO CD. SIN EMBARGO, LOS IMPRIMADORES B Y C CONTIENEN TAMBIEN, EN SUS EXTREMOS 5'', UNA SECUENCIA ADICIONAL CORRESPONDIENTE A TODA O POR LO MENOS A PARTE DE LA SECUENCIA DEL CDR DONADOR. LOS IMPRIMADORES B Y C SE SOLAPAN ENTRE SI UNA LONGITUD SUFICIENTE COMO PARA PERMITIR LA FIJACION POR CALOR DE SUS EXTREMOS 5'' BAJO CONDICIONES QUE PERMITEN QUE SE LLEVE A CABO UNA REACCION EN CADENA DE LA POLIMERASA Y POR TANTO INCORPORAR TODA LA SECUENCIA DEL CDR DONADOR. LAS REGIONES AMPLIFICADAS AB Y CD PUEDEN PASAR POR UNA EXTENSION DE SOLAPAMIENTO DE UNION PARA LA PRODUCCION DEL PRODUCTO QUIMERICO EN UNASOLA REACCION.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB909022011A GB9022011D0 (en) | 1990-10-10 | 1990-10-10 | Chimaeric antibodies and their preparation |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2143466T3 true ES2143466T3 (es) | 2000-05-16 |
Family
ID=10683489
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES91919298T Expired - Lifetime ES2143466T3 (es) | 1990-10-10 | 1991-10-08 | Preparacion de anticuerpos quimericos usando la estrategia pcr recombinante. |
Country Status (9)
Country | Link |
---|---|
US (1) | US5858725A (es) |
EP (1) | EP0552266B1 (es) |
JP (1) | JP3516950B2 (es) |
AT (1) | ATE188995T1 (es) |
DE (1) | DE69131930T2 (es) |
DK (1) | DK0552266T3 (es) |
ES (1) | ES2143466T3 (es) |
GB (1) | GB9022011D0 (es) |
WO (1) | WO1992007075A1 (es) |
Families Citing this family (47)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9115364D0 (en) | 1991-07-16 | 1991-08-28 | Wellcome Found | Antibody |
JP4157160B2 (ja) * | 1991-12-13 | 2008-09-24 | ゾーマ テクノロジー リミテッド | 改変抗体可変領域の調製のための方法 |
US5869619A (en) * | 1991-12-13 | 1999-02-09 | Xoma Corporation | Modified antibody variable domains |
US5817311A (en) * | 1993-03-05 | 1998-10-06 | Universite Catholique De Louvain | Methods of inhibiting T-cell medicated immune responses with LO-CD2a-specific antibodies |
US5498531A (en) * | 1993-09-10 | 1996-03-12 | President And Fellows Of Harvard College | Intron-mediated recombinant techniques and reagents |
US20060257890A1 (en) * | 1996-05-20 | 2006-11-16 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
US6309883B1 (en) * | 1994-02-17 | 2001-10-30 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
US6165793A (en) | 1996-03-25 | 2000-12-26 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US6117679A (en) | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US5837458A (en) | 1994-02-17 | 1998-11-17 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
US6335160B1 (en) * | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
US6406855B1 (en) | 1994-02-17 | 2002-06-18 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
US6395547B1 (en) | 1994-02-17 | 2002-05-28 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US6995017B1 (en) | 1994-02-17 | 2006-02-07 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US6537776B1 (en) | 1999-06-14 | 2003-03-25 | Diversa Corporation | Synthetic ligation reassembly in directed evolution |
US6764835B2 (en) | 1995-12-07 | 2004-07-20 | Diversa Corporation | Saturation mutageneis in directed evolution |
GB9603507D0 (en) * | 1996-02-20 | 1996-04-17 | Isis Innovation | Antibody variants |
US6506602B1 (en) | 1996-03-25 | 2003-01-14 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US20070009930A1 (en) * | 1996-12-18 | 2007-01-11 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
GB9701425D0 (en) | 1997-01-24 | 1997-03-12 | Bioinvent Int Ab | A method for in vitro molecular evolution of protein function |
US6153410A (en) * | 1997-03-25 | 2000-11-28 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
GB9712512D0 (en) | 1997-06-16 | 1997-08-20 | Bioinvent Int Ab | A method for in vitro molecular evolution of protein function |
DE19739685A1 (de) | 1997-09-10 | 1999-03-11 | Eichel Streiber Christoph Von | Monoklonale Antikörper zur Therapie und Prophylaxe von Erkrankungen durch Clostridium difficile |
JP2001518310A (ja) * | 1997-09-29 | 2001-10-16 | シティ・オブ・ホープ | 核酸断片の効率的連結 |
IL136574A0 (en) * | 1997-12-08 | 2001-06-14 | California Inst Of Techn | A method for forming a polynucleotide of desired properties |
BR9906927A (pt) * | 1998-01-14 | 2001-11-20 | Chiron Spa | Proteìnas de neisseria meningitidis |
US7153655B2 (en) * | 1998-06-16 | 2006-12-26 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence |
US6365408B1 (en) | 1998-06-19 | 2002-04-02 | Maxygen, Inc. | Methods of evolving a polynucleotides by mutagenesis and recombination |
WO2001007082A1 (en) | 1999-07-23 | 2001-02-01 | Glaxo Group Limited | Combination of an anti-ep-cam antibody with a chemotherapeutic agent |
US6958213B2 (en) | 2000-12-12 | 2005-10-25 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function |
US20020086292A1 (en) | 2000-12-22 | 2002-07-04 | Shigeaki Harayama | Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules |
US6972324B2 (en) | 2001-05-18 | 2005-12-06 | Boehringer Ingelheim Pharmaceuticals, Inc. | Antibodies specific for CD44v6 |
US20040005709A1 (en) * | 2001-10-24 | 2004-01-08 | Hoogenboom Henricus Renerus Jacobus Mattheus | Hybridization control of sequence variation |
AU2003230027A1 (en) * | 2002-05-17 | 2003-12-02 | Alligator Bioscience Ab | A method for in vitro molecular evolution of protein function |
CN102174533A (zh) | 2002-10-15 | 2011-09-07 | 英特塞尔股份公司 | 编码b族链球菌粘着因子的核酸、b族链球菌的粘着因子和它们的用途 |
GB2432366B (en) * | 2005-11-19 | 2007-11-21 | Alligator Bioscience Ab | A method for in vitro molecular evolution of protein function |
EP2759307B1 (en) | 2006-03-29 | 2016-11-09 | Merial Limited | Vaccine against Streptococci |
WO2009039584A1 (en) | 2007-09-28 | 2009-04-02 | Welcome Receptor Antibodies Pty Ltd | Diagnosis and treatment of diseased and damaged tissue |
ES2883176T3 (es) | 2007-11-08 | 2021-12-07 | Prec Biologics Inc | Anticuerpos monoclonales recombinantes y antígenos correspondientes para cánceres de colon y de páncreas |
WO2010005527A1 (en) | 2008-06-30 | 2010-01-14 | Angioblast Systems, Inc. | Treatment of eye diseases and excessive neovascularization using a combined therapy |
ES2542744T3 (es) * | 2009-12-17 | 2015-08-11 | Novimmune Sa | Bibliotecas de polipéptidos sintéticos y métodos para generar variantes polipeptídicas diversificadas de forma natural |
US9361427B2 (en) * | 2011-02-01 | 2016-06-07 | The Regents Of The University Of California | Scar-less multi-part DNA assembly design automation |
DK2888283T3 (en) | 2012-08-24 | 2018-11-19 | Univ California | ANTIBODIES AND VACCINES FOR TREATING ROR1 CANCER AND INHIBITIVE METASTASE |
EP3169343B1 (en) | 2014-07-15 | 2020-03-25 | Yissum Research and Development Company of the Hebrew University of Jerusalem Ltd. | Isolated polypeptides of cd44 and uses thereof |
JP7109007B2 (ja) | 2016-06-27 | 2022-07-29 | ザ・リージエンツ・オブ・ザ・ユニバーシテイー・オブ・カリフオルニア | がん治療の組み合わせ |
KR20220147684A (ko) * | 2017-05-10 | 2022-11-03 | 더 위스타 인스티튜트 오브 아나토미 앤드 바이올로지 | 최적화된 핵산 항체 작제물 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL162181A (en) * | 1988-12-28 | 2006-04-10 | Pdl Biopharma Inc | A method of producing humanized immunoglubulin, and polynucleotides encoding the same |
-
1990
- 1990-10-08 US US08/039,198 patent/US5858725A/en not_active Expired - Lifetime
- 1990-10-10 GB GB909022011A patent/GB9022011D0/en active Pending
-
1991
- 1991-10-08 DE DE69131930T patent/DE69131930T2/de not_active Expired - Lifetime
- 1991-10-08 EP EP91919298A patent/EP0552266B1/en not_active Expired - Lifetime
- 1991-10-08 ES ES91919298T patent/ES2143466T3/es not_active Expired - Lifetime
- 1991-10-08 JP JP51645691A patent/JP3516950B2/ja not_active Expired - Lifetime
- 1991-10-08 AT AT91919298T patent/ATE188995T1/de not_active IP Right Cessation
- 1991-10-08 DK DK91919298T patent/DK0552266T3/da active
- 1991-10-08 WO PCT/GB1991/001744 patent/WO1992007075A1/en active IP Right Grant
Also Published As
Publication number | Publication date |
---|---|
JP3516950B2 (ja) | 2004-04-05 |
DE69131930D1 (de) | 2000-02-24 |
EP0552266B1 (en) | 2000-01-19 |
ATE188995T1 (de) | 2000-02-15 |
JPH06501614A (ja) | 1994-02-24 |
EP0552266A1 (en) | 1993-07-28 |
DE69131930T2 (de) | 2000-08-03 |
GB9022011D0 (en) | 1990-11-21 |
WO1992007075A1 (en) | 1992-04-30 |
DK0552266T3 (da) | 2000-06-26 |
US5858725A (en) | 1999-01-12 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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