ES2127514T3 - Ensayo de union utilizando agentes de union con grupos cola. - Google Patents

Ensayo de union utilizando agentes de union con grupos cola.

Info

Publication number
ES2127514T3
ES2127514T3 ES95910661T ES95910661T ES2127514T3 ES 2127514 T3 ES2127514 T3 ES 2127514T3 ES 95910661 T ES95910661 T ES 95910661T ES 95910661 T ES95910661 T ES 95910661T ES 2127514 T3 ES2127514 T3 ES 2127514T3
Authority
ES
Spain
Prior art keywords
union
agents
groups
tail groups
cola
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95910661T
Other languages
English (en)
Inventor
Roger Ekins
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Multilyte Ltd
Original Assignee
Multilyte Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Multilyte Ltd filed Critical Multilyte Ltd
Application granted granted Critical
Publication of ES2127514T3 publication Critical patent/ES2127514T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies

Abstract

LA PRESENTE INVENCION DESCRIBE METODOS Y KITS PARA LA DETERMINACION DE LA CONCENTRACION DE UNO O MAS ANALITOS EN UNA MUESTRA DE LIQUIDO QUE UTILIZA AGENTES DE CAPTURA INMOVILIZADOS EN UN SOPORTE SOLIDO Y AGENTES DE UNION PARA UNIR EL ANALITO(S), LOS AGENTES DE UNION TENIENDO GRUPOS DE COLA CAPACES DE UNIRSE AL RESPECTIVO AGENTE DE CAPTURA. PREFERIBLEMENTE, LOS AGENTES DE CAPTURA Y AGENTES DE UNION SON OLIGONUCLEOTIDOS COMPLEMENTARIOS Y LOS AGENTES DE CAPTURA ESTAN INMOVILIZADOS EN LA FORMA DE MICROPUNTOS. LA UTILIZACION DE LOS GRUPOS DE COLA Y AGENTES DE CAPTURA PUEDE PERMITIR QUE LA UNION DEL ANALITO(S) AL AGENTE(S) DE UNION TENGA LUGAR EN UNA SOLUCION, MAS QUE EN UNA SUPERFICIE, MEJORANDO LA CINETICA ASOCIADA CON ESTE PROCESO. ADEMAS, EL USUARIO DEL ENSAYO PUEDE ALTERAR AGENTES DE UNION APROPIADOS PARA UTILIZAR CON UN SOPORTE UNIVERSAL, MEDIANTE LA UNION DE GRUPOS DE COLA A LOS MISMOS.
ES95910661T 1994-03-11 1995-03-10 Ensayo de union utilizando agentes de union con grupos cola. Expired - Lifetime ES2127514T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB9404709A GB9404709D0 (en) 1994-03-11 1994-03-11 Binding assay

Publications (1)

Publication Number Publication Date
ES2127514T3 true ES2127514T3 (es) 1999-04-16

Family

ID=10751658

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95910661T Expired - Lifetime ES2127514T3 (es) 1994-03-11 1995-03-10 Ensayo de union utilizando agentes de union con grupos cola.

Country Status (10)

Country Link
US (3) US6316186B1 (es)
EP (1) EP0749581B1 (es)
JP (2) JP3431160B2 (es)
AU (1) AU1857195A (es)
DE (1) DE69506387T2 (es)
ES (1) ES2127514T3 (es)
FI (1) FI114826B (es)
GB (1) GB9404709D0 (es)
HK (1) HK1014044A1 (es)
WO (1) WO1995024649A1 (es)

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US7157234B2 (en) 1997-10-24 2007-01-02 Beckman Coulter, Inc. Detection of very low quantities of analyte bound to a solid phase
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AU2583899A (en) 1998-02-04 1999-08-23 Invitrogen Corporation Microarrays and uses therefor
US20010031468A1 (en) * 2000-02-08 2001-10-18 Alex Chenchik Analyte assays employing universal arrays
AU5951201A (en) 2000-05-04 2001-11-12 Univ Yale High density protein arrays for screening of protein activity
US9709559B2 (en) 2000-06-21 2017-07-18 Bioarray Solutions, Ltd. Multianalyte molecular analysis using application-specific random particle arrays
DE60117556T2 (de) 2000-06-21 2006-11-02 Bioarray Solutions Ltd. Multianalytische molekularanalyse durch verwendung anwendungsspezifischer zufallspartikelarrays
WO2002033045A2 (en) 2000-10-12 2002-04-25 The Public Health Research Institute Of The City Of New York, Inc. Oligonucleotide - facilitated coalescence
US20070154945A1 (en) * 2001-03-05 2007-07-05 Megumi Akiyama Immobilized biomolecule and method of detecting substance capable of interacting with biomolecule
US20030082571A1 (en) * 2001-04-10 2003-05-01 Kachab Edward Hanna Linear nucleic acid and sequence therefor
US7262063B2 (en) 2001-06-21 2007-08-28 Bio Array Solutions, Ltd. Directed assembly of functional heterostructures
US20040161741A1 (en) 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
US9777312B2 (en) 2001-06-30 2017-10-03 Enzo Life Sciences, Inc. Dual polarity analysis of nucleic acids
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
NZ532947A (en) 2001-10-15 2006-01-27 Bioarray Solutions Ltd Multiplexed analysis of polymorphic loci by concurrent interrogation and enzyme-mediated detection
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
US7041453B2 (en) * 2002-08-22 2006-05-09 Bioarray Solutions Ltd. Molecular constructs and methods of use for detection of biochemical reactions
AU2003298655A1 (en) 2002-11-15 2004-06-15 Bioarray Solutions, Ltd. Analysis, secure access to, and transmission of array images
US7229763B2 (en) * 2003-04-07 2007-06-12 Beckman Coulter, Inc. Assay system using labeled oligonucleotides
US20040229226A1 (en) * 2003-05-16 2004-11-18 Reddy M. Parameswara Reducing microarray variation with internal reference spots
WO2005029705A2 (en) 2003-09-18 2005-03-31 Bioarray Solutions, Ltd. Number coding for identification of subtypes of coded types of solid phase carriers
ATE532066T1 (de) 2003-09-22 2011-11-15 Bioarray Solutions Ltd Oberflächenimmobilisierter polyelektrolyt mit mehreren, zur kovalenten bindung an biomoleküle fähigen funktionellen gruppen
WO2005042763A2 (en) 2003-10-28 2005-05-12 Bioarray Solutions Ltd. Optimization of gene expression analysis using immobilized capture probes
EP1694859B1 (en) 2003-10-29 2015-01-07 Bioarray Solutions Ltd Multiplexed nucleic acid analysis by fragmentation of double-stranded dna
US20080254997A1 (en) * 2004-03-18 2008-10-16 Nissui Pharmaceutical Co., Ltd. Kit, Device and Method For Analyzing Biological Substance
US7364898B2 (en) * 2004-05-04 2008-04-29 Eppendorf Ag Customized micro-array construction and its use for target molecule detection
US7848889B2 (en) 2004-08-02 2010-12-07 Bioarray Solutions, Ltd. Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification
GB0421838D0 (en) 2004-09-30 2004-11-03 Congenia S R L Cancer markers
US8486629B2 (en) 2005-06-01 2013-07-16 Bioarray Solutions, Ltd. Creation of functionalized microparticle libraries by oligonucleotide ligation or elongation
WO2007126101A1 (ja) * 2006-04-28 2007-11-08 Toppan Printing Co., Ltd. 蛋白質チップ
WO2008016680A1 (en) * 2006-08-02 2008-02-07 California Institute Of Technology Methods and systems for detecting and/or sorting targets
US20090032446A1 (en) * 2007-08-01 2009-02-05 Triwatech, L.L.C. Mobile station and methods for diagnosing and modeling site specific effluent treatment facility requirements
SE533515C2 (sv) 2008-04-16 2010-10-12 Aamic Ab Analysförfarande för samtidig analys av flera analyter
US8309306B2 (en) * 2008-11-12 2012-11-13 Nodality, Inc. Detection composition
EP2389585A2 (en) * 2009-01-22 2011-11-30 Li-Cor, Inc. Single molecule proteomics with dynamic probes
GB201016403D0 (en) 2010-09-29 2010-11-10 Bath Ventures Novel interaction between staphylococcus aureus Sbi and C3d protiens
GB201021909D0 (en) 2010-12-23 2011-02-02 Randox Lab Ltd Multi-analyte microarrays using tag-specific antibodies and tag-anchored antibodies
US20200385792A1 (en) * 2018-01-05 2020-12-10 Quotient Suisse Sa Self-assembling diagnostic array platform
EP3811079A1 (en) 2018-06-21 2021-04-28 F. Hoffmann-La Roche AG Hybridizing all-lna oligonucleotides
BR112021009419A2 (pt) * 2018-11-16 2021-08-17 F. Hoffmann-La Roche Ag fase sólida, método de preparação de uma fase sólida, uso, kit para determinar um analito em uma amostra, complexo e métodos para formar um complexo e para determinar um analito em uma amostra
CN113939599A (zh) * 2019-06-07 2022-01-14 豪夫迈·罗氏有限公司 杂交全lna寡核苷酸

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Also Published As

Publication number Publication date
FI114826B (fi) 2004-12-31
US6316186B1 (en) 2001-11-13
EP0749581B1 (en) 1998-12-02
GB9404709D0 (en) 1994-04-27
DE69506387T2 (de) 1999-05-12
US20060204983A1 (en) 2006-09-14
US7588893B2 (en) 2009-09-15
FI963560A0 (fi) 1996-09-10
HK1014044A1 (en) 1999-09-17
JP3619222B2 (ja) 2005-02-09
US20020182617A1 (en) 2002-12-05
AU1857195A (en) 1995-09-25
WO1995024649A1 (en) 1995-09-14
DE69506387D1 (de) 1999-01-14
JP2003156493A (ja) 2003-05-30
JPH10512664A (ja) 1998-12-02
JP3431160B2 (ja) 2003-07-28
EP0749581A1 (en) 1996-12-27
FI963560A (fi) 1996-09-10

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