ES2126262T3 - Cassette de expresion que comprende una region promotora salida del gen pho4 de schizosaccharomyces pombe. - Google Patents

Cassette de expresion que comprende una region promotora salida del gen pho4 de schizosaccharomyces pombe.

Info

Publication number
ES2126262T3
ES2126262T3 ES95911383T ES95911383T ES2126262T3 ES 2126262 T3 ES2126262 T3 ES 2126262T3 ES 95911383 T ES95911383 T ES 95911383T ES 95911383 T ES95911383 T ES 95911383T ES 2126262 T3 ES2126262 T3 ES 2126262T3
Authority
ES
Spain
Prior art keywords
pct
schizosaccharomyces pombe
expression cassette
gene
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95911383T
Other languages
English (en)
Inventor
Eric Jacobs
Nathalie Silvestre
Ernst Schweingruber
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Transgene SA
Original Assignee
Transgene SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transgene SA filed Critical Transgene SA
Application granted granted Critical
Publication of ES2126262T3 publication Critical patent/ES2126262T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

LA PRESENTE INVENCION SE REFIERE AL USO COMBINADO DE DOS CASILLAS DE EXPRESION, PERMITIENDO UNA LA EXPRESION DE UN FRAGMENTO DE ADN DE INTERES BAJO EL CONTROL DE UNA REGION PROMOTORA REGULABLE POR LA TIANINA Y PERMITIENDO LA OTRA LA EXPRESION DE UN GEN ACTIVADOR, ASI COMO LAS CELULAS HUESPEDES EN LAS QUE SE PONE EN PRACTICA TAL USO. LA PRESENTE INVENCION SE REFIERE TAMBIEN A UNA CASILLA DE EXPRESION EN LA QUE EL FRAGMENTO DE ADN DE INTERES Y BAJO EL CONTROL DE UNA REGION PROMOTORA OBTENIDA DEL GEN PHO-4 DE SCHIZOSACCHAROMYCES POMBE Y LOS VECTORES Y CELULAS QUE COMPRENDEN TAL CASILLA DE EXPRESION. POR FIN, LA INVENCION COMPRENDE UN NUEVO PROCEDIMIENTO DE PRODUCCION DE UNA PROTEINA DE INTERES.
ES95911383T 1994-03-10 1995-03-09 Cassette de expresion que comprende una region promotora salida del gen pho4 de schizosaccharomyces pombe. Expired - Lifetime ES2126262T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9402767A FR2717187B1 (fr) 1994-03-10 1994-03-10 Usage combiné de deux cassettes d'expression pour la production d'une protéine d'intérêt.

Publications (1)

Publication Number Publication Date
ES2126262T3 true ES2126262T3 (es) 1999-03-16

Family

ID=9460880

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95911383T Expired - Lifetime ES2126262T3 (es) 1994-03-10 1995-03-09 Cassette de expresion que comprende una region promotora salida del gen pho4 de schizosaccharomyces pombe.

Country Status (12)

Country Link
US (1) US5834237A (es)
EP (1) EP0749489B1 (es)
JP (1) JPH09509843A (es)
AT (1) ATE174630T1 (es)
AU (1) AU700159B2 (es)
CA (1) CA2185103A1 (es)
DE (1) DE69506691T2 (es)
DK (1) DK0749489T3 (es)
ES (1) ES2126262T3 (es)
FR (1) FR2717187B1 (es)
GR (1) GR3029565T3 (es)
WO (1) WO1995024491A1 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6709858B1 (en) 1997-11-03 2004-03-23 The Arizona Board Of Regents On Behalf Of The University Of Arizona Hyperthermic inducible expression vectors for gene therapy and methods of use thereof
WO1999023216A2 (en) * 1997-11-03 1999-05-14 The Arizona Board Of Regents On Behalf Of The University Of Arizona Hyperthermic inducible expression vectors for gene therapy and methods of use thereof
US7098191B2 (en) 1997-11-03 2006-08-29 The Arizona Board Of Reagents Hyperthermic inducible expression vectors for gene therapy and methods of use thereof
WO2001059091A2 (en) * 2000-02-11 2001-08-16 Metabolix, Inc. Multi-gene expression constructs containing modified inteins
US7300792B2 (en) * 2002-04-04 2007-11-27 University Of Tennessee Research Foundation Lux expression in eukaryotic cells
CA2965865C (en) 2002-07-18 2021-10-19 Merus N.V. Recombinant production of mixtures of antibodies
USRE47770E1 (en) 2002-07-18 2019-12-17 Merus N.V. Recombinant production of mixtures of antibodies
EP2395016A3 (en) 2003-05-30 2012-12-19 Merus B.V. Design and use of paired variable regions of specific binding molecules
US20100069614A1 (en) 2008-06-27 2010-03-18 Merus B.V. Antibody producing non-human mammals
DK1737971T3 (da) * 2004-01-20 2017-11-13 Merus Nv Blandinger af bindingsproteiner
AU2013249985B2 (en) 2012-04-20 2017-11-23 Merus N.V. Methods and means for the production of Ig-like molecules
SG11201408646VA (en) 2012-07-06 2015-01-29 Genmab Bv Dimeric protein with triple mutations
WO2020243660A1 (en) 2019-05-30 2020-12-03 490 BioTech, Inc. Lux expression in cells and methods of use

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1236844B (it) * 1989-10-19 1993-04-22 Mini Ricerca Scient Tecnolog Clonaggio del gene regolabile nmt1 di schizosaccharomyces pombe e impiego delle sequenze di controllo della regolazione della trascrizione di tale gene per l'espressione regolabile di geni eterologhi

Also Published As

Publication number Publication date
JPH09509843A (ja) 1997-10-07
DK0749489T3 (da) 1999-08-23
EP0749489A1 (fr) 1996-12-27
DE69506691D1 (de) 1999-01-28
FR2717187B1 (fr) 1996-05-31
GR3029565T3 (en) 1999-06-30
AU1896895A (en) 1995-09-25
WO1995024491A1 (fr) 1995-09-14
DE69506691T2 (de) 1999-06-10
US5834237A (en) 1998-11-10
ATE174630T1 (de) 1999-01-15
EP0749489B1 (fr) 1998-12-16
FR2717187A1 (fr) 1995-09-15
CA2185103A1 (fr) 1995-09-14
AU700159B2 (en) 1998-12-24

Similar Documents

Publication Publication Date Title
ES2126262T3 (es) Cassette de expresion que comprende una region promotora salida del gen pho4 de schizosaccharomyces pombe.
FI950628A0 (fi) Modifioidut sienisolut ja menetelmä rekombinanttituotteiden tuottamiseksi
NO942456L (no) Fremgangsmåte for fremstilling av trombin
DE69638334D1 (de) Methode für die produktion von retroviralen verpackungszellinien, die einen retroviralen überstand mit hocheffizienter transduktionsrate produzieren
FI950409A (fi) Yhdistelmä soluja, DNA-rakenteita, vektoreita ja menetelmiä fytaattia hajoittavien entsyymien ilmentämiseksi toivotuissa suhteissa
DK609985D0 (da) Adhesin-antigener, antistoffer mod disse og dna-fragmenter, som indkoder antigenerne
DK0770139T3 (da) En svamp, hvor areA-genet er blevet modificeret, samt et areA-gen fra aspergillus oryzae
AR001678A1 (es) Variantes de glucanotransferasa de ciclomaltodextrina construccion de adn vector de expresion recombinante celula huesped método para producir variantes usos de dichas variantes método para modificar la adhesion y/o especialmente de la enzima CGTasa precursora
AU4940096A (en) Host cell expressing reduced levels of a metalloprotease and methods using the host cell in protein production
ATE474926T1 (de) Verwendung von clya hemolysin fur ausscheidung von fusionprotein
DK0910642T3 (da) Fremgangsmåde til at öge hæmoproteinproduktionen i filamentöse fungi
ATE386130T1 (de) Adenovirale vektoren für die gentherapie
SE9501472D0 (sv) Truncated IGF-I
BR0005798A (pt) Processo para preparação fermentativa del-aminoácidos usando bactérias corineforme
ATE413451T1 (de) Methoden zur massenkultivierung von zellen die rekombinantes menschliches erythropoietin produzieren
Strid et al. Kinetics of the membrane‐bound inorganic pyrophosphatase from Rhodospirillum rubrum chromatophores: Effect of the transmembrane electrical potential on the rate constants
HUT63196A (en) Process for producing and applying new cloning and expression vectors
AU6433886A (en) A method for the expression of genes in yeast, and DNA fragments as well as plasmids comprising said DNA fragments to be used when carrying out the method
KR850007980A (ko) 인체 내인성 암 조절인자의 제조방법
PT788549E (pt) Sequencias reguladoras de acidos nucleicos e utilizacoes
NZ332555A (en) Gene expression in monocytes and macrophages associated with the CD68 gene
ATE264387T1 (de) Verfahren zur auslösung von apoptose in zellen
MX9700255A (es) Metodo para identificar acidos nucleicos que codifican las proteinas activadoras del promotor c-fos.

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 749489

Country of ref document: ES