ES2118916T3 - Procedimiento de amplificacion de arn con una sola etapa de manipulacion. - Google Patents

Procedimiento de amplificacion de arn con una sola etapa de manipulacion.

Info

Publication number
ES2118916T3
ES2118916T3 ES93401119T ES93401119T ES2118916T3 ES 2118916 T3 ES2118916 T3 ES 2118916T3 ES 93401119 T ES93401119 T ES 93401119T ES 93401119 T ES93401119 T ES 93401119T ES 2118916 T3 ES2118916 T3 ES 2118916T3
Authority
ES
Spain
Prior art keywords
arn
innatural
adnc
synthesizing
reagents
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93401119T
Other languages
English (en)
Inventor
Francois Mallet
Guy Oriol
Bernard Mandrand
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux SA
Original Assignee
Biomerieux SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux SA filed Critical Biomerieux SA
Application granted granted Critical
Publication of ES2118916T3 publication Critical patent/ES2118916T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PROCESO DE AMPLIFICACION ARN, COMPORTANDO LAS ETAPAS QUE CONSISTEN, EN DESNATURALIZAR EL ARN POR CALENTAMIENTO DE UNA SOLUCION QUE LE CONTIENE, EN SINTETIZAR UNA PRIMERA PIEZA DE ADNC A PARTIR DE UN CEBADO APROPIADO EN PRESENCIA DE UNA TRANSCRIPTASA INVERTIDA, EN DESNATURALIZAR EL HETERODUPLEX FORMADO, EN SINTETIZAR LA SEGUNDA PIEZA DE ADNC A PARTIR DE UN SEGUNDO CEBADO EN PRESENCIA DE ADN POLIMERASA, LUEGO EN SOMETER EL ADNC OBTENIDO A UNOS CICLOS DE AMPLIFICACION EN NUMERO SUFICIENTE. GRACIAS AL DESCUBRIMIENTO QUE CIERTAS TRANSCRIPTASAS INVERTIDAS NO REPUTADAS TERMOESTABLES SON CAPACES, CUANDO SON UTILIZADAS EN CANTIDAD SUFICIENTE, DE RESISTIR UN TRATAMIENTO DE DESNATURALIZACION DEL ARN POR EL CALOR A UNA TEMPERATURA SUPERIOR O IGUAL A 60 C, Y AUN PODER FUNCIONAR, DESPUES DE ESTE TRATAMIENTO, A TEMPERATURAS ELEVADAS QUE CORRESPONDEN A CONDICIONES SUFICIENTEMENTE DISCRIMINANTES, ESTE PROCESO SE CARACTERIZA POR EL HECHO QUE DICHA SOLUCION DE ARN CONTIENE DESDE LA PARTIDA, TODOS LOS REACTIVOS Y EN UN MISMO CONTENEDOR SIN AÑADIDO DE REACTIVOS O DISOLVENTES. ES ASI POSIBLE OBRAR EN UNAS CONDICIONES SATISFACTORIAS, EN UNA SOLA ETAPA DE MANIPULACION, LO QUE EVITA NOTABLEMENTE LOS RIESGOS DE CONTAMINACION. APLICACION EN ESPECIAL AL DIAGNOSTICO DE LAS INFECCIONES BACTERIANAS O VIRICAS Y A LOS DESORDENES GENETICOS, ASI COMO AL TIPO CELULAR.
ES93401119T 1992-04-29 1993-04-29 Procedimiento de amplificacion de arn con una sola etapa de manipulacion. Expired - Lifetime ES2118916T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9205322A FR2690691B1 (fr) 1992-04-29 1992-04-29 Procede d'amplification d'arn necessitant une seule etape de manipulation.

Publications (1)

Publication Number Publication Date
ES2118916T3 true ES2118916T3 (es) 1998-10-01

Family

ID=9429395

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93401119T Expired - Lifetime ES2118916T3 (es) 1992-04-29 1993-04-29 Procedimiento de amplificacion de arn con una sola etapa de manipulacion.

Country Status (6)

Country Link
US (2) US5654143A (es)
EP (1) EP0569272B1 (es)
CA (1) CA2095070C (es)
DE (1) DE69319180T2 (es)
ES (1) ES2118916T3 (es)
FR (1) FR2690691B1 (es)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2690691B1 (fr) * 1992-04-29 1999-02-12 Bio Merieux Procede d'amplification d'arn necessitant une seule etape de manipulation.
USRE39031E1 (en) * 1992-04-29 2006-03-21 Biomerieux RNA amplification method requiring only one manipulation step
US5660979A (en) * 1994-11-04 1997-08-26 Akzo Nobel N.V. Detection of human retrovirus infection
DE19513728A1 (de) * 1995-04-11 1996-10-31 Boehringer Mannheim Gmbh Verfahren zur Reduzierung der Bildung von Artefakten
US6013488A (en) * 1996-07-25 2000-01-11 The Institute Of Physical And Chemical Research Method for reverse transcription
EP2354251B1 (en) 1997-04-03 2014-10-15 Life Technologies Corporation Compositons and methods for reverse transcriptase-Polymerase chain reaction (RT-PCR)
DE69841241D1 (de) * 1997-04-22 2009-11-26 Life Technologies Corp Verfahren zur herstellung von aslv reverser transkriptase zusammengestellt aus mehreren untereinheiten
KR19990064373A (ko) * 1998-06-24 1999-08-05 김필동 Dna를 이용한 제품의 장식방법
GB9915027D0 (en) * 1999-06-29 1999-08-25 Wilkinson Jonathan F A formulation of reverse transcriptases with enhanced efficiency of RNA amplification
US6232104B1 (en) 1999-08-17 2001-05-15 Dade Behring Inc. Detection of differences in nucleic acids by inhibition of spontaneous DNA branch migration
US7118860B2 (en) 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
US6893819B1 (en) * 2000-11-21 2005-05-17 Stratagene California Methods for detection of a nucleic acid by sequential amplification
US7838225B2 (en) * 1999-10-29 2010-11-23 Hologic, Inc. Methods for detection of a target nucleic acid by forming a cleavage structure using a reverse transcriptase
US7824859B2 (en) * 1999-10-29 2010-11-02 Cytyc Corporation Methods for detection of a target nucleic acid by forming a cleavage structure using an RNA polymerase
GB9928232D0 (en) * 1999-12-01 2000-01-26 Skelton Stephen Detection system
US20030022318A1 (en) * 2000-01-25 2003-01-30 Epiclone, Inc. Method for thermocycling amplification of nucleic acid sequences and the generation of related peptides thereof
WO2001066797A2 (en) * 2000-03-03 2001-09-13 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Multiplex hybridization system for identification of pathogenic mycobacterium and method of use
US6379932B1 (en) * 2000-07-17 2002-04-30 Incyte Genomics, Inc. Single primer PCR amplification of RNA
US20080242627A1 (en) * 2000-08-02 2008-10-02 University Of Southern California Novel rna interference methods using dna-rna duplex constructs
US7678541B2 (en) * 2000-11-21 2010-03-16 Hologic, Inc. Methods and compositions for the detection of a nucleic acid using a non-invasive cleavage reaction
WO2002044736A2 (en) 2000-11-30 2002-06-06 Molecular Skincare Limited Diagnosis and treatment of epidermal or skin diseases
US7338805B2 (en) 2001-05-04 2008-03-04 Bio Merieux Labeling reagents, methods for synthesizing such reagents and methods for detecting biological molecules
US7060441B2 (en) 2001-05-04 2006-06-13 Biomerieux Method for fragmenting and labeling DNA involving abasic sites and phosphate labeling
US20030021799A1 (en) * 2001-07-11 2003-01-30 Wilkinson Jonathan Frome Reverse transcriptase formulations
FR2868071B1 (fr) 2004-03-26 2006-06-09 Biomerieux Sa Reactifs de marquage, procedes de synthese de tels reactifs et procedes de detection de molecules biologiques
FR2886735B1 (fr) 2005-06-01 2015-09-11 Biomerieux Sa Procede de marquage ou de traitement d'un echantillon biologique contenant des molecules biologiques d'interet, notamment des acides nucleiques
FR2902430B1 (fr) 2005-11-25 2012-11-02 Biomerieux Sa Oligonucleotides, utilisation, methode de detection et kit permettant de diagnostiquer la presence des genes h5 et n1 du virus d'influenza a
FR2906532B1 (fr) 2006-09-28 2008-12-12 Biomerieux Sa Nouvel oligonucleotide marque
EP1956097A1 (en) 2007-02-06 2008-08-13 bioMerieux B.V. Method for discriminating single nucleotide polymorphisms (SNPs)
FR2917090B1 (fr) 2007-06-11 2012-06-15 Biomerieux Sa Reactifs de marquage portant des fonctions diazo et nitro, procedes de synthese de tels reactifs et procedes de detection de molecules biologiques
EP2071034A1 (en) 2007-12-12 2009-06-17 bioMérieux Method for treating a solution in order to destroy any ribonucleic acid after amplification
FR2933410B1 (fr) 2008-07-04 2010-08-20 Biomerieux Sa Nouvelle sonde de detection
FR2934595B1 (fr) 2008-07-29 2013-04-05 Biomerieux Sa Reactifs de marquage ayant un noyau pyridine portant une fonction diazomethyle, procedes de synthese de tels reactifs et procedes de detection de molecules biologiques
EP2172563A1 (en) 2008-09-24 2010-04-07 bioMérieux S.A. Method for lowering the dependency towards sequence variation of a nucleic acid target in a diagnostic hybridization assay
FR2940805B1 (fr) 2009-01-05 2015-10-16 Biomerieux Sa Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede
FR2950358B1 (fr) 2009-09-18 2015-09-11 Biomerieux Sa Dispositif d'amplification d'acides nucleiques simplifie et son procede de mise en oeuvre
TWI518325B (zh) 2010-02-04 2016-01-21 自治醫科大學 對ａｌｋ抑制劑具有先抗性或後抗性癌症的識別、判斷和治療
US10174373B2 (en) 2012-10-18 2019-01-08 Idexx Laboratories, Inc. Nucleic acid amplification controls and kits and methods of use thereof

Family Cites Families (12)

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Publication number Priority date Publication date Assignee Title
GB8423659D0 (en) * 1984-09-19 1984-10-24 Pasteur Institut Cloned dna sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5021335A (en) * 1988-06-17 1991-06-04 The Board Of Trustees Of The Leland Stanford Junior University In situ transcription in cells and tissues
US5229297A (en) * 1989-02-03 1993-07-20 Eastman Kodak Company Containment cuvette for PCR and method of use
EP0397463A3 (en) * 1989-05-10 1991-03-06 Eastman Kodak Company Construction of synthetic double-stranded dna sequences
US5219727A (en) * 1989-08-21 1993-06-15 Hoffmann-Laroche Inc. Quantitation of nucleic acids using the polymerase chain reaction
IL95825A (en) * 1989-10-02 1994-12-29 Union Oil Co Production of tetraacycarbonate acid salts in aqueous solution
DE69030386T2 (de) * 1989-12-22 1997-10-09 F. Hoffmann-La Roche Ag, Basel Bei hoher temperatur aktive reverse transkriptasen
JPH04141098A (ja) * 1990-09-29 1992-05-14 Shimadzu Corp Ｒｎａ検出用試薬及びそれを用いたｒｎａの検出方法
FR2669347A1 (fr) * 1990-11-15 1992-05-22 Inst Nat Sante Rech Med Procede permettant d'effectuer au moins deux reactions successives, en evitant les risques de contamination, notamment applicable aux techniques d'amplification de l'adn ou de l'arn.
FR2690691B1 (fr) * 1992-04-29 1999-02-12 Bio Merieux Procede d'amplification d'arn necessitant une seule etape de manipulation.

Also Published As

Publication number Publication date
DE69319180T2 (de) 1999-02-11
US5817465A (en) 1998-10-06
CA2095070C (fr) 2000-01-25
EP0569272A1 (fr) 1993-11-10
CA2095070A1 (fr) 1993-10-30
FR2690691A1 (fr) 1993-11-05
FR2690691B1 (fr) 1999-02-12
EP0569272B1 (fr) 1998-06-17
US5654143A (en) 1997-08-05
DE69319180D1 (de) 1998-07-23

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