ES2118120T3 - Procedimiento de inmovilizacion de un fragmento de acido nucleico por fijacion pasiva sobre un soporte solido, soporte solido asi obtenido y su utilizacion. - Google Patents

Procedimiento de inmovilizacion de un fragmento de acido nucleico por fijacion pasiva sobre un soporte solido, soporte solido asi obtenido y su utilizacion.

Info

Publication number
ES2118120T3
ES2118120T3 ES92402080T ES92402080T ES2118120T3 ES 2118120 T3 ES2118120 T3 ES 2118120T3 ES 92402080 T ES92402080 T ES 92402080T ES 92402080 T ES92402080 T ES 92402080T ES 2118120 T3 ES2118120 T3 ES 2118120T3
Authority
ES
Spain
Prior art keywords
solid support
support
nucleic acid
supply
passive fixation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92402080T
Other languages
English (en)
Inventor
Philippe Cros
Patrice Andre Allibert
Bernard Fabien Mandrand
Pascal Thierry Dalbon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux SA
Original Assignee
Biomerieux SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux SA filed Critical Biomerieux SA
Application granted granted Critical
Publication of ES2118120T3 publication Critical patent/ES2118120T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Abstract

PROCESO DE INMOVILIZACION POR FIJACION PASIVA, SOBRE UN SOPORTE SOLIDO, DE UN FRAGMENTO DE ACIDO NUCLEICO QUE COMPRENDE MENOS DE 100 NUCLEOTIDOS, EN EL QUE SE DEPOSITA SOBRE DICHO SOPORTE DICHO FRAGMENTO EN FORMA DE UN DERIVADO RESULTANTE DEL ACOPLAMIENTO COVALENTE DE DICHO FRAGMENTO CON UN LIGANDO QUE TIENE UNA MASA MOLECULAR INFERIOR A 5000 Y QUE COMPRENDEN AL MENOS UN GRUPO FUNCIONAL POLAR, NO SIENDO DICHO DERIVADO SUSCEPTIBLE DE REACCIONAR SOBRE DICHO SOPORTE CON FORMACION DE UN ENLACE COVALENTE EN LAS CONDICIONES DE DICHO DEPOSITO, ENTENDIENDOSE QUE CUANDO DICHO LIGANDO ES UN NUCLEOTIDO U OLIGONUCLEOTIDO, COMPRENDE AL MENOS UN NUCLEOTIDO MODIFICADO DE MANERA A INTRODUCIR DICHO GRUPO FUNCIONAL POLAR, Y SOPORTE SOLIDO ASI OBTENIDO. ESTE SOPORTE PUEDE UTILIZARSE PARTICULARMENTE COMO SONDA DE CAPTURA PARA LA DETECCION DE UN BLANCO NUCLEOTIDICO.
ES92402080T 1991-07-17 1992-07-17 Procedimiento de inmovilizacion de un fragmento de acido nucleico por fijacion pasiva sobre un soporte solido, soporte solido asi obtenido y su utilizacion. Expired - Lifetime ES2118120T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9109057A FR2679255B1 (fr) 1991-07-17 1991-07-17 Procede d'immobilisation d'un fragment nucleique par fixation passive sur un support solide, support solide ainsi obtenu et son utilisation.

Publications (1)

Publication Number Publication Date
ES2118120T3 true ES2118120T3 (es) 1998-09-16

Family

ID=9415243

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92402080T Expired - Lifetime ES2118120T3 (es) 1991-07-17 1992-07-17 Procedimiento de inmovilizacion de un fragmento de acido nucleico por fijacion pasiva sobre un soporte solido, soporte solido asi obtenido y su utilizacion.

Country Status (9)

Country Link
US (1) US5510084A (es)
EP (1) EP0524864B1 (es)
JP (1) JP2797047B2 (es)
AU (1) AU659176B2 (es)
CA (1) CA2074135C (es)
DE (1) DE69225646T2 (es)
ES (1) ES2118120T3 (es)
FI (1) FI102619B (es)
FR (1) FR2679255B1 (es)

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FR2706618B1 (fr) * 1993-06-11 1995-09-01 Bio Merieux Dispositif pour le dosage d'haptènes et son utilisation.
FR2707010B1 (es) * 1993-06-25 1995-09-29 Bio Merieux
US5656427A (en) * 1994-08-29 1997-08-12 Gen-Probe Incorporated Nucleic acid hybridization assay probes, helper probes and amplification oligonucleotides targeted to Mycoplasma pneumoniae nucleic acid
FR2726277B1 (fr) 1994-10-28 1996-12-27 Bio Merieux Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin
GB9425138D0 (en) * 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
WO1996019587A2 (en) * 1994-12-22 1996-06-27 Abbott Laboratories Methods of immobilizing oligonucleotides to solid support materials and methods of using support bound oligonucleotides
FR2733755B1 (fr) * 1995-05-03 1997-07-11 Bio Merieux Fragment nucleotidique de l'arn ribosomique 16s de corynebacteries, sondes et amorces derivees, reactif et procede de detection
FR2758884B1 (fr) * 1997-01-30 1999-04-02 Bio Merieux Procede pour isoler, notamment detecter ou quantifier un analyte dans un milieu
US6692912B1 (en) 1997-03-05 2004-02-17 Matrix Technologies Corporation Nucleic acid-containing polymerizable complex
US5932711A (en) * 1997-03-05 1999-08-03 Mosaic Technologies, Inc. Nucleic acid-containing polymerizable complex
IL124275A (en) * 1997-05-02 2002-03-10 Bio Merieux Vitek Inc A method to produce sequences of nucleic acids
US6410275B1 (en) 1997-05-02 2002-06-25 Biomerieux, Inc. Disposable test devices for performing nucleic acid amplification reactions
US6429007B1 (en) 1997-05-02 2002-08-06 BIOMéRIEUX, INC. Nucleic acid amplification reaction station for disposable test devices
US6558901B1 (en) 1997-05-02 2003-05-06 Biomerieux Vitek Nucleic acid assays
US5786182A (en) * 1997-05-02 1998-07-28 Biomerieux Vitek, Inc. Dual chamber disposable reaction vessel for amplification reactions, reaction processing station therefor, and methods of use
US5989499A (en) * 1997-05-02 1999-11-23 Biomerieux, Inc. Dual chamber disposable reaction vessel for amplification reactions
US6762019B2 (en) * 1997-09-30 2004-07-13 Surmodics, Inc. Epoxide polymer surfaces
US6465178B2 (en) 1997-09-30 2002-10-15 Surmodics, Inc. Target molecule attachment to surfaces
US5858653A (en) * 1997-09-30 1999-01-12 Surmodics, Inc. Reagent and method for attaching target molecules to a surface
US6703228B1 (en) 1998-09-25 2004-03-09 Massachusetts Institute Of Technology Methods and products related to genotyping and DNA analysis
EP1001037A3 (en) * 1998-09-28 2003-10-01 Whitehead Institute For Biomedical Research Pre-selection and isolation of single nucleotide polymorphisms
US20050153926A1 (en) * 1998-12-04 2005-07-14 Adams Christopher P. Method for the immobilization of oligonucleotides
WO2000034343A1 (en) * 1998-12-04 2000-06-15 Mosaic Technologies, Inc. Method for the immobilization of oligonucleotides
US6383783B1 (en) 1999-09-21 2002-05-07 3M Innovative Properties Company Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant
GB0016836D0 (en) * 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (1)
US6706479B2 (en) * 2000-10-05 2004-03-16 Virginia Tech Intellectual Properties, Inc. Bio-chip, photoluminescent methods for identifying biological material, and apparatuses for use with such methods and bio-chips
CA2473376A1 (en) 2002-01-16 2003-07-31 Dynal Biotech Asa Method for isolating nucleic acids and protein from a single sample
US20040067495A1 (en) * 2002-10-08 2004-04-08 Ray Gustav Allen Polynucleic acid and polyamino acid binding substrate
US7309593B2 (en) * 2003-10-01 2007-12-18 Surmodics, Inc. Attachment of molecules to surfaces
US20050130177A1 (en) * 2003-12-12 2005-06-16 3M Innovative Properties Company Variable valve apparatus and methods
US7939249B2 (en) 2003-12-24 2011-05-10 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and concentration step
US7727710B2 (en) 2003-12-24 2010-06-01 3M Innovative Properties Company Materials, methods, and kits for reducing nonspecific binding of molecules to a surface
EP1623996A1 (en) * 2004-08-06 2006-02-08 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Improved method of selecting a desired protein from a library
US8053246B2 (en) * 2006-02-23 2011-11-08 National University Corporation Hokkaido University Method of esterifying bio-related molecule for mass spectrometry and method of mass spectrometry of obtained esterified derivative
US20100129878A1 (en) * 2007-04-25 2010-05-27 Parthasarathy Ranjani V Methods for nucleic acid amplification
EP2408791A1 (en) * 2009-03-19 2012-01-25 SEPS Pharma N.V. Fosfluconazole derivatives, synthesis, and use in long acting formulations
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Also Published As

Publication number Publication date
FR2679255B1 (fr) 1993-10-22
FR2679255A1 (fr) 1993-01-22
US5510084A (en) 1996-04-23
EP0524864B1 (fr) 1998-05-27
FI102619B1 (fi) 1999-01-15
JP2797047B2 (ja) 1998-09-17
DE69225646T2 (de) 1998-12-24
AU659176B2 (en) 1995-05-11
EP0524864A1 (fr) 1993-01-27
CA2074135A1 (fr) 1993-01-18
CA2074135C (fr) 2001-04-17
FI923260A0 (fi) 1992-07-16
JPH05271272A (ja) 1993-10-19
FI102619B (fi) 1999-01-15
FI923260A (fi) 1993-01-18
DE69225646D1 (de) 1998-07-02
AU2037992A (en) 1993-01-21

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