ES2111000T3 - Separacion de proteinas y colorantes. - Google Patents

Separacion de proteinas y colorantes.

Info

Publication number
ES2111000T3
ES2111000T3 ES91916462T ES91916462T ES2111000T3 ES 2111000 T3 ES2111000 T3 ES 2111000T3 ES 91916462 T ES91916462 T ES 91916462T ES 91916462 T ES91916462 T ES 91916462T ES 2111000 T3 ES2111000 T3 ES 2111000T3
Authority
ES
Spain
Prior art keywords
protein
dye
dye separation
dowex
eluant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91916462T
Other languages
English (en)
Inventor
R A Johnson
Alan Victor Quirk
John Rodney Woodrow
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Albumedix Ltd
Original Assignee
Delta Biotechnology Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Delta Biotechnology Ltd filed Critical Delta Biotechnology Ltd
Application granted granted Critical
Publication of ES2111000T3 publication Critical patent/ES2111000T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

PROBLEMA: CUANDO LAS PROTEINAS SE PURIFICAN USANDO UN TINTE DE UNION A LAS PROTEINAS INMOVILIZADOS SOBRE UNA MATRIZ CROMATOGRAFICA, EL TINTE O UNA PARTE/DERIVADO DEL MISMO PUEDE ESCAPARSE AL INTERIOR DEL ELUYENTE. SOLUCION: SE USAN UNA RESINA DE INTERCAMBIO DE IONES (POR EJ. DOWEX-1) Y UN MATERIAL DISLURUPTOR (POR EJ. SAL Y ACIDO GRASO TAL COMO OCTANOATO DE SODIO) PARA SEPARAR EL TINTE DE LA PROTEINA.
ES91916462T 1990-09-12 1991-09-12 Separacion de proteinas y colorantes. Expired - Lifetime ES2111000T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB909019919A GB9019919D0 (en) 1990-09-12 1990-09-12 Purification of proteins

Publications (1)

Publication Number Publication Date
ES2111000T3 true ES2111000T3 (es) 1998-03-01

Family

ID=10682072

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91916462T Expired - Lifetime ES2111000T3 (es) 1990-09-12 1991-09-12 Separacion de proteinas y colorantes.

Country Status (13)

Country Link
EP (1) EP0548191B1 (es)
JP (1) JP2983287B2 (es)
AT (1) ATE159726T1 (es)
AU (1) AU644687B2 (es)
CA (1) CA2089161C (es)
DE (1) DE69128088T2 (es)
DK (1) DK0548191T3 (es)
ES (1) ES2111000T3 (es)
GB (2) GB9019919D0 (es)
GR (1) GR3025758T3 (es)
IE (1) IE913226A1 (es)
WO (1) WO1992004367A1 (es)
ZA (1) ZA917274B (es)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5625041A (en) * 1990-09-12 1997-04-29 Delta Biotechnology Limited Purification of proteins
US5728553A (en) * 1992-09-23 1998-03-17 Delta Biotechnology Limited High purity albumin and method of producing
AU4837996A (en) * 1996-02-29 1997-09-16 Delta Biotechnology Limited High purity albumin production process
GB9902000D0 (en) 1999-01-30 1999-03-17 Delta Biotechnology Ltd Process
GB0217033D0 (en) 2002-07-23 2002-08-28 Delta Biotechnology Ltd Gene and polypeptide sequences
DE10304066B4 (de) * 2003-01-31 2007-01-18 Henkel Kgaa Verfahren zur Veredelung konzentrierter Enzymlösungen
GB0329681D0 (en) 2003-12-23 2004-01-28 Delta Biotechnology Ltd Gene expression technique
GB0329722D0 (en) 2003-12-23 2004-01-28 Delta Biotechnology Ltd Modified plasmid and use thereof
FR2866890B1 (fr) 2004-02-27 2008-04-04 Lab Francais Du Fractionnement Procede de purification d'albumine comprenant une etape de nanofiltration, solution et composition a usage therapeutique la contenant
JP5631533B2 (ja) 2004-12-23 2014-11-26 ノボザイムス バイオファーマ デーコー アクティーゼルスカブ 遺伝子発現技術
EP2201036A1 (en) 2007-08-08 2010-06-30 Novozymes Biopharma DK A/S Transferrin variants and conjugates
SG172789A1 (en) 2009-02-11 2011-08-29 Novozymes Biopharma Dk As Albumin variants and conjugates
BR112012009450A2 (pt) 2009-10-30 2017-05-23 Novozymes Biopharma Dk As variantes de albumina
EP2780364A2 (en) 2011-11-18 2014-09-24 Eleven Biotherapeutics, Inc. Proteins with improved half-life and other properties
CA2890766A1 (en) 2012-11-08 2014-05-15 Novozymes Biopharma Dk A/S Albumin variants
CA2989966C (en) 2015-08-20 2024-04-30 Albumedix A/S Albumin variants and conjugates
CN108431204A (zh) 2015-12-22 2018-08-21 阿尔布梅迪克斯医疗有限公司 改良的表达蛋白质的菌株
JP7146637B2 (ja) 2015-12-22 2022-10-04 アルブミディクス リミティド 改善されたタンパク質発現株
CA3064037A1 (en) 2017-06-20 2018-12-27 Albumedix Ltd Improved protein expression strains

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2342058A1 (de) * 1973-08-20 1975-02-27 Ts I Gematologii I Pereliwanij Verfahren zur isolierung des reinen serumalbumins aus biologischen fluessigkeiten
GB2053926B (en) * 1979-07-20 1983-02-23 Atkinson A Albumin extraction by affinity chromatography
GB2058926B (en) * 1979-09-20 1983-07-20 Consolidated Pneumatic Tool Co Closed loop compressor system
JPS6383100A (ja) * 1986-09-26 1988-04-13 Green Cross Corp:The ヒト尿中アルブミンの製造方法
DE3911629A1 (de) * 1989-04-10 1990-10-11 Behringwerke Ag Verfahren zur abtrennung von toxinen aus proteinloesungen
JP3230091B2 (ja) * 1990-06-25 2001-11-19 ウェルファイド株式会社 ヒト血清アルブミンの着色抑制方法

Also Published As

Publication number Publication date
GB9019919D0 (en) 1990-10-24
GB2263279A (en) 1993-07-21
GB9302665D0 (en) 1993-05-12
CA2089161C (en) 1999-12-14
CA2089161A1 (en) 1992-03-13
DE69128088D1 (de) 1997-12-04
IE913226A1 (en) 1992-02-25
ZA917274B (en) 1992-07-29
EP0548191A1 (en) 1993-06-30
WO1992004367A1 (en) 1992-03-19
ATE159726T1 (de) 1997-11-15
JP2983287B2 (ja) 1999-11-29
GB2263279B (en) 1994-01-26
JPH06500110A (ja) 1994-01-06
EP0548191B1 (en) 1997-10-29
AU8505791A (en) 1992-03-30
DK0548191T3 (da) 1998-03-16
AU644687B2 (en) 1993-12-16
DE69128088T2 (de) 1998-03-19
GR3025758T3 (en) 1998-03-31

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