ES2109387T3 - Procedimiento para la deteccion de acidos nucleicos. - Google Patents

Procedimiento para la deteccion de acidos nucleicos.

Info

Publication number
ES2109387T3
ES2109387T3 ES93105916T ES93105916T ES2109387T3 ES 2109387 T3 ES2109387 T3 ES 2109387T3 ES 93105916 T ES93105916 T ES 93105916T ES 93105916 T ES93105916 T ES 93105916T ES 2109387 T3 ES2109387 T3 ES 2109387T3
Authority
ES
Spain
Prior art keywords
procedure
immobilized
detection
nucleic acids
acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93105916T
Other languages
English (en)
Other versions
ES2109387T5 (es
Inventor
Cornelia Dr Kruse-Muller
Stefanie Dr Kohler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=6456845&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2109387(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Application granted granted Critical
Publication of ES2109387T3 publication Critical patent/ES2109387T3/es
Publication of ES2109387T5 publication Critical patent/ES2109387T5/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Communicable Diseases (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Surgical Instruments (AREA)
  • Electrophonic Musical Instruments (AREA)
  • Golf Clubs (AREA)
  • Saccharide Compounds (AREA)

Abstract

PROCEDIMIENTO PARA COMPROBAR UN ACIDO NUCLEINICO A TRAVES DEL DESARROLLO INDIVIDUAL DEL ACIDO NUCLEINICO EN CONDICIONES ALCALINAS. POR LO MENOS ESTA PRESENTE UN DETERGENTE DEL GRUPO DE DETERGENTES ANIONICOS NO IONICAS E HIBRIDAS SE ADICIONA UNA SONDA DE RETENCION INMOVILIZADA O NO INMOVILIZADA AL ACIDO NUCLEINICO, SE HIBRIDA LA SONDA DE RETENCION CON EL ACIDO NUCLEINICO INMOVILIZANDO EL ACIDO NUCLEINICO POR LA SONDA DE RETENCION Y SE COMPRUEBA LA CANTIDAD DE HIBRIDO INMOVILIZADO FORMADO.
ES93105916T 1992-04-15 1993-04-13 Procedimiento para la deteccion de acidos nucleicos. Expired - Lifetime ES2109387T5 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4212555 1992-04-15
DE4212555A DE4212555A1 (de) 1992-04-15 1992-04-15 Verfahren zum Nachweis von Nukleinsäuren

Publications (2)

Publication Number Publication Date
ES2109387T3 true ES2109387T3 (es) 1998-01-16
ES2109387T5 ES2109387T5 (es) 2009-01-01

Family

ID=6456845

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93105916T Expired - Lifetime ES2109387T5 (es) 1992-04-15 1993-04-13 Procedimiento para la deteccion de acidos nucleicos.

Country Status (8)

Country Link
US (1) US6066455A (es)
EP (1) EP0566050B2 (es)
JP (1) JP2638421B2 (es)
AT (1) ATE157707T1 (es)
AU (1) AU654771B2 (es)
DE (2) DE4212555A1 (es)
ES (1) ES2109387T5 (es)
FI (1) FI931677A (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4344742A1 (de) * 1993-06-09 1994-12-15 Boehringer Mannheim Gmbh Verfahren zur Immobilisierung von Nukleinsäuren
US7569342B2 (en) 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
NL1010224C2 (nl) * 1998-09-30 2000-03-31 Packard Biosciene B V Werkwijze voor het detecteren van ATP.
EP2243832A3 (en) * 2005-02-18 2011-01-05 Gen-Probe Incorporated Sample preparation method incorporating an alkaline shock
EP1861721B1 (en) 2005-03-10 2017-05-03 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes within samples
US20110065108A1 (en) * 2008-02-01 2011-03-17 Siemens Healthcare Diagnostics Inc. Urine Transport Medium
US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
US8718948B2 (en) 2011-02-24 2014-05-06 Gen-Probe Incorporated Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector
EP2764112B1 (en) * 2011-10-05 2018-04-18 Spartan Bioscience Inc. Direct nucleic acid analysis
EP3387107B1 (en) 2015-12-11 2020-08-12 Spartan Bioscience Inc. Tube sealing system and methods for nucleic acid amplification

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI63596C (fi) * 1981-10-16 1983-07-11 Orion Yhtymae Oy Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser
GB8317855D0 (en) * 1983-06-30 1983-08-03 Iq Bio Ltd Biochemical detection method
US4652517A (en) * 1984-06-11 1987-03-24 Diagnostic Research Limited Partnership Methods for the in vitro detection and identification of unknown pathogens or genetic entities
DK171161B1 (da) * 1985-03-28 1996-07-08 Hoffmann La Roche Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve
NO870613L (no) * 1986-03-05 1987-09-07 Molecular Diagnostics Inc Deteksjon av mikroorganismer i en prŸve inneholdende nukleinsyre.
CA1338457C (en) * 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
EP0261955A3 (en) * 1986-09-26 1989-06-07 E.I. Du Pont De Nemours And Company Process for immobilization of dna
AU601021B2 (en) * 1987-03-11 1990-08-30 Molecular Diagnostics, Inc. Assay for necleic acid sequences in a sample
US4886741A (en) * 1987-12-09 1989-12-12 Microprobe Corporation Use of volume exclusion agents for the enhancement of in situ hybridization
US5225326A (en) * 1988-08-31 1993-07-06 Research Development Foundation One step in situ hybridization assay
US5232829A (en) * 1989-09-29 1993-08-03 Hoffmann-La Roche Inc. Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes
US5231015A (en) * 1989-10-18 1993-07-27 Eastman Kodak Company Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme
CA2048302A1 (en) * 1990-08-15 1992-02-16 Victoria P. Meucci Solubilization reagent for biological test samples
WO1992018649A1 (en) * 1991-04-12 1992-10-29 Microprobe Corporation Compositions and methods for improved extraction and hybridization of nucleic acid
WO1993020234A1 (en) * 1992-03-31 1993-10-14 E.I. Du Pont De Nemours And Company A rapid, high capacity nucleic acid based assay

Also Published As

Publication number Publication date
FI931677A0 (fi) 1993-04-14
JP2638421B2 (ja) 1997-08-06
FI931677A (fi) 1993-10-16
ATE157707T1 (de) 1997-09-15
AU654771B2 (en) 1994-11-17
DE59307233D1 (de) 1997-10-09
ES2109387T5 (es) 2009-01-01
US6066455A (en) 2000-05-23
AU3679293A (en) 1993-11-04
EP0566050B1 (de) 1997-09-03
EP0566050B2 (de) 2008-07-09
JPH0622797A (ja) 1994-02-01
DE4212555A1 (de) 1993-10-21
EP0566050A1 (de) 1993-10-20

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