ES2097806T3 - Procedimiento para purificar un polipeptido. - Google Patents

Procedimiento para purificar un polipeptido.

Info

Publication number
ES2097806T3
ES2097806T3 ES91906510T ES91906510T ES2097806T3 ES 2097806 T3 ES2097806 T3 ES 2097806T3 ES 91906510 T ES91906510 T ES 91906510T ES 91906510 T ES91906510 T ES 91906510T ES 2097806 T3 ES2097806 T3 ES 2097806T3
Authority
ES
Spain
Prior art keywords
purify
polypeptide
procedure
container
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91906510T
Other languages
English (en)
Inventor
Toshihiko Kaminuma
Toshii Iida
Masahiro Tajima
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shiseido Co Ltd
Original Assignee
Shiseido Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shiseido Co Ltd filed Critical Shiseido Co Ltd
Application granted granted Critical
Publication of ES2097806T3 publication Critical patent/ES2097806T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

UN PROCESO MEJORADO PARA PURIFICAR UN POLIPEPTIDO CON UN ENVASE PARA LA CROMATOGRAFIA DE LIQUIDOS DE ALTO RENDIMIENTO QUE CONSISTE EN PRETRATAR LOS POLIPEPTIDOS PRODUCIDOS POR VARIAS CELULAS EN UN ESTADO DADO, AJUSTANDO EL PH DE UNA SOLUCION ACUOSA DE LOS POLIPEPTIDOS PARA QUE CAIGA DENTRO DE UN MARGEN ESPECIFICO PARA ASI EXTRAER LOS CONTAMINANTES MEDIANTE PRECIPITACION Y TRATAR LA SOLUCION QUE QUEDA CON UN ENVASE PARA LA CROMATOGRAFIA DE LIQUIDOS DE ALTO RENDIMIENTO EN FASE INVERSA.
ES91906510T 1990-03-30 1991-03-29 Procedimiento para purificar un polipeptido. Expired - Lifetime ES2097806T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP8689890 1990-03-30
JP21301690 1990-08-10

Publications (1)

Publication Number Publication Date
ES2097806T3 true ES2097806T3 (es) 1997-04-16

Family

ID=26427972

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91906510T Expired - Lifetime ES2097806T3 (es) 1990-03-30 1991-03-29 Procedimiento para purificar un polipeptido.

Country Status (8)

Country Link
US (1) US5612454A (es)
EP (1) EP0480048B1 (es)
KR (1) KR100203545B1 (es)
AU (1) AU645794B2 (es)
CA (1) CA2057014C (es)
DE (1) DE69124561T2 (es)
ES (1) ES2097806T3 (es)
WO (1) WO1991015502A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1254370B (it) * 1992-05-22 1995-09-14 Sorin Biomedica Spa Procedimento per la purificazione di proteine da sistemi cellulari.
US7495070B2 (en) * 2000-04-24 2009-02-24 Yale University Protein binding miniature proteins
US6966992B2 (en) * 2001-03-19 2005-11-22 Akzo Nobel Nv Method of purification of molecules using unbranched terminal alkyldiols
US6822073B2 (en) * 2001-12-20 2004-11-23 Kimberly-Clark Worldwide, Inc. Modular peptide-based reagent
EP2895610B1 (en) 2012-09-13 2019-11-06 Indiana University Research and Technology Corporation Compositions and systems for conferring disease resistance in plants and methods of use thereof
WO2014063194A1 (en) 2012-10-23 2014-05-01 The University Of Sydney Elastic hydrogel
CN105246520B (zh) 2012-12-10 2017-11-21 埃拉斯塔根私人有限公司 可缩放的三维弹性构建体制造
US20160194379A1 (en) 2013-08-13 2016-07-07 Elastagen Pty Ltd Regeneration of Damaged Tissue
EP3049427A4 (en) * 2013-09-24 2017-05-31 Elastagen Pty Ltd Method of extracting protein

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60123500A (ja) * 1983-12-08 1985-07-02 Mitsubishi Petrochem Co Ltd 新規カルシトニン及びその採取法
EP0252588A3 (en) * 1986-05-12 1989-07-12 Smithkline Beecham Corporation Process for the isolation and purification of p. falciparum cs protein expressed in recombinant e. coli, and its use as a vaccine
US4894330A (en) * 1986-12-23 1990-01-16 Cetus Corporation Purification of recombinant beta-interferon incorporating RP-HPLC

Also Published As

Publication number Publication date
DE69124561T2 (de) 1997-09-04
EP0480048A1 (en) 1992-04-15
KR100203545B1 (ko) 1999-06-15
WO1991015502A1 (en) 1991-10-17
CA2057014A1 (en) 1991-10-01
EP0480048A4 (en) 1993-04-28
EP0480048B1 (en) 1997-02-05
DE69124561D1 (de) 1997-03-20
AU645794B2 (en) 1994-01-27
CA2057014C (en) 2001-07-24
US5612454A (en) 1997-03-18
AU7548291A (en) 1991-10-30

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