ES2094821T3 - Metodo para la determinacion de endotoxina. - Google Patents
Metodo para la determinacion de endotoxina.Info
- Publication number
- ES2094821T3 ES2094821T3 ES91916941T ES91916941T ES2094821T3 ES 2094821 T3 ES2094821 T3 ES 2094821T3 ES 91916941 T ES91916941 T ES 91916941T ES 91916941 T ES91916941 T ES 91916941T ES 2094821 T3 ES2094821 T3 ES 2094821T3
- Authority
- ES
- Spain
- Prior art keywords
- addition
- specimen
- endotoxin
- analyzing
- limulus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/579—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
SE DESCRIBE UN METODO PARA ANALIZAR ENDOTOXINAS DE MANERA EXACTA PARA PERMITIR ASI UN DIAGNOSTICO DEFINITORIO DE SEPSIS BACTERIAL GRAMNEGATIVA, QUE HASTA AHORA HA SIDO MUY DIFICIL EFECTUARLO RAPIDAMENTE, MEDIANTE LA ADICION DE UNA SOLUCION ACUOSA DE UNA MEZCLA DE UN SUPERFICIATIVO ESPECIFICADO, UN COMPUESTO QUE CONTIENE IMIDAZOLILO O GRUPO AMINO, UNA SAL METALICA DE TIERRA ALCALINA Y UN HIDROXIDO DE METAL ALCALINO A UN ESPECIMEN Y LA ADICION POSTERIOR DE UN COMPONENTE DE LISATO AMEBOCITO LIMULUS. SE PUEDE PREPARAR UNA EXCELENTE SOLUCION DE ESPECIMEN DADO QUE UNA ENDOTOXINA ADHERENTE A UNA PROTEINA, LIPIDO, PLAQUETA, ETC. PUEDE LIBERARSE EFICAZMENTE SIMPLEMENTE AÑADIENDO LA SOLUCION ACUOSA QUE NO DEBE ESTAR SUJETA A VARIAS OPERACIONES INCLUIDA LA SEPARACION. POR TANTO ESTE METODO SIRVE PARA SOLUCIONAR EL PROBLEMA DEL METODO ANTERIOR DE ANALIZAR ENDOTOXINA MEDIANTE EL TEST LIMULUS UTILIZANDO PLASMA, SUERO, ETC., COMO UN ESPECIMEN QUE NECESITA PRETRATAMIENTO COMO SEPARACION CENTRIFUGA DE PRECIPITADOS DESNATURALIZADOS FORMADOS POR UN TRATAMIENTO ACIDO, DE MANERA QUE LA OPERACION SEA COMPLICADA.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2257415A JP2957251B2 (ja) | 1990-09-28 | 1990-09-28 | エンドトキシンの測定法 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2094821T3 true ES2094821T3 (es) | 1997-02-01 |
Family
ID=17306062
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES91916941T Expired - Lifetime ES2094821T3 (es) | 1990-09-28 | 1991-09-27 | Metodo para la determinacion de endotoxina. |
Country Status (6)
Country | Link |
---|---|
US (1) | US5378610A (es) |
EP (1) | EP0513361B1 (es) |
JP (1) | JP2957251B2 (es) |
DE (1) | DE69123445T2 (es) |
ES (1) | ES2094821T3 (es) |
WO (1) | WO1992006382A1 (es) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2737514B2 (ja) * | 1992-01-24 | 1998-04-08 | 和光純薬工業株式会社 | エンドトキシン測定用試料の前処理方法 |
JP3524120B2 (ja) * | 1992-05-08 | 2004-05-10 | 生化学工業株式会社 | 前処理剤、前処理方法、前処理された試料による測定法、測定用キット及び試料の判定方法 |
JP3242733B2 (ja) * | 1993-02-26 | 2001-12-25 | 生化学工業株式会社 | エンドトキシン特異的測定剤 |
JP3429036B2 (ja) * | 1993-09-30 | 2003-07-22 | 生化学工業株式会社 | エンドトキシンの特異的測定剤 |
JP3061418B2 (ja) * | 1993-11-22 | 2000-07-10 | 生化学工業株式会社 | リムルス試薬反応性物質の測定方法 |
US5648230A (en) * | 1994-06-30 | 1997-07-15 | Seikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation) | Endotoxin stabilizing agent, endotoxin composition and method for assaying endotoxin |
US5855832A (en) * | 1996-06-27 | 1999-01-05 | Clausi; Robert N. | Method of molding powdered plant fiber into high density materials |
EP0837330B1 (en) * | 1996-10-21 | 2005-08-24 | Seikagaku Corporation | Method for measuring Endotoxin |
US20050079566A1 (en) * | 2003-09-10 | 2005-04-14 | Caterer Nigel Robert | Analytical methods for determination of proteolytic cleavage at specific sites |
US7638340B2 (en) | 2005-06-22 | 2009-12-29 | Seikagu Corporation | Method of eliminating reactivity of lipoarabinomannan and application of the same |
ES2816550T3 (es) * | 2012-12-28 | 2021-04-05 | Seikagaku Kogyo Co Ltd | Método de pre-tratamiento para antitrombina III que se va a someter a una prueba de Limulus |
RS58167B1 (sr) | 2015-07-28 | 2019-03-29 | Hoffmann La Roche | Unapređeni bakterijski endotoksinski test za određivanje endotoksina |
CN112462076B (zh) * | 2020-11-12 | 2022-10-18 | 天津华龛生物科技有限公司 | 应用于微载体的内毒素含量的检测方法 |
CN112462015B (zh) * | 2020-11-18 | 2022-07-12 | 海南倍特药业有限公司 | 一种氢溴酸瑞马唑仑细菌内毒素的检测方法 |
CN113607525B (zh) * | 2021-08-09 | 2024-06-14 | 安徽信灵检验医学科技股份有限公司 | 一种用于血液消解的真空采血管添加剂及其制备方法 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4096091A (en) * | 1974-07-17 | 1978-06-20 | Baxter Travenol Laboratories, Inc. | Limulus lysate having improved capacity to precipitate in the presence of low endotoxin concentrations, and reconstituting solutions therefor |
US4221866A (en) * | 1978-10-06 | 1980-09-09 | Baxter Travenol Laboratories, Inc. | Method for determining endotoxin concentration |
US4276050A (en) * | 1980-01-10 | 1981-06-30 | Abbott Laboratories | Method for systemic endotoxin detection |
BE889407A (fr) * | 1980-06-27 | 1981-10-16 | Mallinckrodt Inc | Amelioration du procede de preparation d'un lysat de limule |
US4322217A (en) * | 1980-06-27 | 1982-03-30 | Mallinckrodt, Inc. | Process for preparing Limulus lysate |
US4406832A (en) * | 1981-09-03 | 1983-09-27 | Mallinckrodt, Inc. | Peptide-type substrates useful in the quantitative determination of endotoxin |
JPS5885162A (ja) * | 1981-11-16 | 1983-05-21 | Seikagaku Kogyo Co Ltd | エンドトキシン測定法 |
US4808314A (en) * | 1987-09-18 | 1989-02-28 | Scripps Clinic And Research Foundation | Method for reducing bacterial endotoxin contamination in solutions of macromolecules |
-
1990
- 1990-09-28 JP JP2257415A patent/JP2957251B2/ja not_active Expired - Fee Related
-
1991
- 1991-09-27 EP EP91916941A patent/EP0513361B1/en not_active Expired - Lifetime
- 1991-09-27 WO PCT/JP1991/001309 patent/WO1992006382A1/ja active IP Right Grant
- 1991-09-27 US US07/859,398 patent/US5378610A/en not_active Expired - Lifetime
- 1991-09-27 DE DE69123445T patent/DE69123445T2/de not_active Expired - Fee Related
- 1991-09-27 ES ES91916941T patent/ES2094821T3/es not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
EP0513361A4 (en) | 1992-07-21 |
DE69123445D1 (de) | 1997-01-16 |
EP0513361B1 (en) | 1996-12-04 |
JPH04136763A (ja) | 1992-05-11 |
US5378610A (en) | 1995-01-03 |
EP0513361A1 (en) | 1992-11-19 |
WO1992006382A1 (en) | 1992-04-16 |
DE69123445T2 (de) | 1997-04-24 |
JP2957251B2 (ja) | 1999-10-04 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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