ES2081848T3 - Composiciones a base de conjugados de una enzima y de una hidrazona o hidrazina de un acido nucleico, sondas y metodos. - Google Patents

Composiciones a base de conjugados de una enzima y de una hidrazona o hidrazina de un acido nucleico, sondas y metodos.

Info

Publication number
ES2081848T3
ES2081848T3 ES89309532T ES89309532T ES2081848T3 ES 2081848 T3 ES2081848 T3 ES 2081848T3 ES 89309532 T ES89309532 T ES 89309532T ES 89309532 T ES89309532 T ES 89309532T ES 2081848 T3 ES2081848 T3 ES 2081848T3
Authority
ES
Spain
Prior art keywords
nucleic acid
hydrazone
hydrazine
conjugates
probes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES89309532T
Other languages
English (en)
Inventor
Soumitra Shankar Ghosh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siska Diagnostics Inc
Original Assignee
Siska Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Siska Diagnostics Inc filed Critical Siska Diagnostics Inc
Application granted granted Critical
Publication of ES2081848T3 publication Critical patent/ES2081848T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Hematology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

SE PROVEE UNA MUESTRA DE ACIDO NUCLEICO, EN DONDE LA ETIQUETA ES UN ENCIMA CATALITICAMENTE ACTIVO. EL ACIDO NUCLEICO DE LA INVESTIGACION ES UN DNA O UN RNA Y COMPRENDE UN SEGMENTO DE AL MENOS 20 NUCLEOTIDOS EN LA SECUENCIA COMPLEMENTARIA AL DEL SEGMENTO OBJETIVO DE LA INVESTIGACION. EN LA PRUEBA, EL ACIDO NUCLEICO Y EL ENCIMA ESTAN UNIDOS COVALENTEMENTE, MEDIANTE UN ENLACE, EL CUAL ESTA LIGADO EN UN EXTREMO, AL 5''-CARBONO DEL NUCLEOTIDO 5''DEL ACIDO NUCLEICO Y, EN EL OTRO EXTREMO, AL ENCIMA, Y EL CUAL COMPRENDE UN GRUPO HIDRAZONA O HIDRACINA. TAMBIEN SE PROVEE UN ACIDO NUCLEICO, EL CUAL ES UN INTERMEDIO PARA HACER UNA MUESTRA DE ACUERDO CON LA INVENCION Y EL CUAL DERIVA EN EL CARBONO 5'' DEL NUCLEOTIDO 5'', CON LA MITAD DE UN GRUPO HIDRACINO. ADEMAS SE PROVEEN METODOS DE HACER UNA MUESTRA DEL ACIDO NUCLEICO DE LA INVENCION. EN UNO DE TALES METODOS, UN ACIDO NUCLEICO DE LA INVENCION SE HACE REACCIONAR CON UN ENCIMA, EL CUAL ESTA MODIFICADO, TENIENDO UN GRUPO ALDEHIDO. EN OTRO DE TALES METODOS, UNA MUESTRA DE LA INVENCION, CON UNA HIDRACINA EN EL ENLACE DE UNION ENTRE EL ACIDO NUCLEICO Y EL ENCIMA, SE REDUCE CON CIANOBOROHIDRURO; TAL REDUCCION CAMBIA LA HIDRAZONA A UNA HIDRACINA PERO DE OTRO MODO NO SE AFECTA SUBSTANCIALMENTE A LA MUESTRA. ADEMAS, SE PROVEE UN METODO PARA ANALIZAR UN ACIDO NUCLEICO EXTRAYENDO UNA MUESTRA DE ACIDO NUCLEICO DEL ENSAYO DE HIBRIDACION, ESTANDO LA MUESTRA PARA EL ANALISIS DE ACUERDO CON LA INVENCION. FINALMENTE, LA INVENCION PROVEE EL COMPUESTO BENCILAMIDOBENZALDEHIDO 4-N'' Y MUESTRAS DE ACIDO NUCLEICO ETIQUETADAS CON 2,4-DINITROFENILO O 4-N''-BENCILAMIDOFENILO MEDIANTE UN LIGANTE EL CUAL COMPRENDE UN GRUPO HIDRAZONA O HIDRACINA.
ES89309532T 1988-09-26 1989-09-19 Composiciones a base de conjugados de una enzima y de una hidrazona o hidrazina de un acido nucleico, sondas y metodos. Expired - Lifetime ES2081848T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US24976688A 1988-09-26 1988-09-26

Publications (1)

Publication Number Publication Date
ES2081848T3 true ES2081848T3 (es) 1996-03-16

Family

ID=22944901

Family Applications (1)

Application Number Title Priority Date Filing Date
ES89309532T Expired - Lifetime ES2081848T3 (es) 1988-09-26 1989-09-19 Composiciones a base de conjugados de una enzima y de una hidrazona o hidrazina de un acido nucleico, sondas y metodos.

Country Status (6)

Country Link
EP (2) EP0361768B1 (es)
JP (1) JPH02257897A (es)
AT (1) ATE130046T1 (es)
CA (1) CA1333366C (es)
DE (1) DE68924750T2 (es)
ES (1) ES2081848T3 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992008808A1 (en) * 1990-11-14 1992-05-29 Siska Diagnostics, Inc. Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor
WO1995005480A2 (en) * 1993-08-18 1995-02-23 Id Biomedical Corporation Compositions and methods for detecting target nucleic acid sequences utilizing flanking sequence enzyme molecules
GB9512380D0 (en) * 1995-06-17 1995-08-16 Cruachem Ltd Labelling and detection of nucleic acids
WO2001051689A1 (en) * 2000-01-11 2001-07-19 Nanogen Recognomics Gmbh Biomolecules having multiple attachment moieties for binding to a substrate surface
CA2723664A1 (en) 2000-03-22 2001-09-27 Solulink, Incorporated Hydrazine-based and carbonyl-based bifunctional crosslinking reagents
US7102024B1 (en) 2000-08-01 2006-09-05 Schwartz David A Functional biopolymer modification reagents and uses thereof
US6686461B1 (en) 2000-03-22 2004-02-03 Solulink Bioscience, Inc. Triphosphate oligonucleotide modification reagents and uses thereof
US7129229B2 (en) 2000-08-11 2006-10-31 Nanogen Recognomics Gmbh Hydrazide building blocks and hydrazide modified biomolecules
EP1385997A2 (en) * 2001-04-09 2004-02-04 Fraunhofer-Gesellschaft Zur Förderung Der Angewandten Forschung E.V. System and method for detecting and quantifying nucleic acids using covalent binding
GB201003594D0 (en) * 2010-03-04 2010-04-21 Eluceda Ltd Method for detecting the presence of specific micro-organisms and device for the same

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4537718A (en) * 1982-10-26 1985-08-27 Columbia University In The City Of New York Impermeant spectroscopic probes
US4605735A (en) * 1983-02-14 1986-08-12 Wakunaga Seiyaku Kabushiki Kaisha Oligonucleotide derivatives
FR2565996B1 (fr) * 1984-06-15 1988-01-22 Inst Nat Sante Rech Med Acides nucleiques marques chimiquement, leur utilisation et necessaire pour sa mise en oeuvre
JPS622164A (ja) * 1985-06-25 1987-01-08 シスカ・ダイアグノステイツクス・インコ−ポレ−テツド Ｎ４−（置換アミノ）シチジンを含む核酸プロ−ブ
CA1295559C (en) * 1985-08-13 1992-02-11 Jannis Stavrianopoulos Method for labeling polynucleotide sequences

Also Published As

Publication number Publication date
JPH02257897A (ja) 1990-10-18
EP0361768A3 (en) 1991-06-05
DE68924750T2 (de) 1996-05-09
EP0361768A2 (en) 1990-04-04
ATE130046T1 (de) 1995-11-15
EP0670329A2 (en) 1995-09-06
CA1333366C (en) 1994-12-06
DE68924750D1 (de) 1995-12-14
EP0670329A3 (en) 1995-12-06
EP0361768B1 (en) 1995-11-08

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