DE68924750T2 - Enzym-Nukleinsäurehydrazon und Enzym-Nukleinsäurehydrazid-Konjugat-Zusammensetzung, Sonden und Verfahren. - Google Patents
Enzym-Nukleinsäurehydrazon und Enzym-Nukleinsäurehydrazid-Konjugat-Zusammensetzung, Sonden und Verfahren.Info
- Publication number
- DE68924750T2 DE68924750T2 DE68924750T DE68924750T DE68924750T2 DE 68924750 T2 DE68924750 T2 DE 68924750T2 DE 68924750 T DE68924750 T DE 68924750T DE 68924750 T DE68924750 T DE 68924750T DE 68924750 T2 DE68924750 T2 DE 68924750T2
- Authority
- DE
- Germany
- Prior art keywords
- nucleic acid
- enzyme
- probes
- methods
- hydrazone
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Hematology (AREA)
- Genetics & Genomics (AREA)
- Urology & Nephrology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US24976688A | 1988-09-26 | 1988-09-26 |
Publications (2)
Publication Number | Publication Date |
---|---|
DE68924750D1 DE68924750D1 (de) | 1995-12-14 |
DE68924750T2 true DE68924750T2 (de) | 1996-05-09 |
Family
ID=22944901
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE68924750T Expired - Fee Related DE68924750T2 (de) | 1988-09-26 | 1989-09-19 | Enzym-Nukleinsäurehydrazon und Enzym-Nukleinsäurehydrazid-Konjugat-Zusammensetzung, Sonden und Verfahren. |
Country Status (6)
Country | Link |
---|---|
EP (2) | EP0670329A3 (de) |
JP (1) | JPH02257897A (de) |
AT (1) | ATE130046T1 (de) |
CA (1) | CA1333366C (de) |
DE (1) | DE68924750T2 (de) |
ES (1) | ES2081848T3 (de) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5474895A (en) * | 1990-11-14 | 1995-12-12 | Siska Diagnostics Inc. | Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor |
JPH09506762A (ja) * | 1993-08-18 | 1997-07-08 | アイディー バイオメディカル コーポレイション | 近接配列−酵素分子を利用して標的核酸配列を検出するための組成物および方法 |
GB9512380D0 (en) * | 1995-06-17 | 1995-08-16 | Cruachem Ltd | Labelling and detection of nucleic acids |
KR20020065644A (ko) | 2000-01-11 | 2002-08-13 | 나노겐 인코포레이티드 | 기판 표면 결합용의 다수의 부착 잔기를 갖는 생분자 |
EP1315699B1 (de) | 2000-03-22 | 2013-01-02 | Solulink, Incorporated | Hydrazin- und carbonyl-basierte bifunktionelle vernetzungsmittel |
US6686461B1 (en) | 2000-03-22 | 2004-02-03 | Solulink Bioscience, Inc. | Triphosphate oligonucleotide modification reagents and uses thereof |
US7102024B1 (en) | 2000-08-01 | 2006-09-05 | Schwartz David A | Functional biopolymer modification reagents and uses thereof |
US7129229B2 (en) | 2000-08-11 | 2006-10-31 | Nanogen Recognomics Gmbh | Hydrazide building blocks and hydrazide modified biomolecules |
WO2002081738A2 (en) * | 2001-04-09 | 2002-10-17 | Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E.V. | System and method for detecting and quantifying nucleic acids using covalent binding |
GB201003594D0 (en) * | 2010-03-04 | 2010-04-21 | Eluceda Ltd | Method for detecting the presence of specific micro-organisms and device for the same |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4537718A (en) * | 1982-10-26 | 1985-08-27 | Columbia University In The City Of New York | Impermeant spectroscopic probes |
US4605735A (en) * | 1983-02-14 | 1986-08-12 | Wakunaga Seiyaku Kabushiki Kaisha | Oligonucleotide derivatives |
FR2565996B1 (fr) * | 1984-06-15 | 1988-01-22 | Inst Nat Sante Rech Med | Acides nucleiques marques chimiquement, leur utilisation et necessaire pour sa mise en oeuvre |
JPS622164A (ja) * | 1985-06-25 | 1987-01-08 | シスカ・ダイアグノステイツクス・インコ−ポレ−テツド | N4−(置換アミノ)シチジンを含む核酸プロ−ブ |
CA1295559C (en) * | 1985-08-13 | 1992-02-11 | Jannis Stavrianopoulos | Method for labeling polynucleotide sequences |
-
1989
- 1989-08-31 CA CA000610032A patent/CA1333366C/en not_active Expired - Fee Related
- 1989-09-19 EP EP95105491A patent/EP0670329A3/de not_active Withdrawn
- 1989-09-19 ES ES89309532T patent/ES2081848T3/es not_active Expired - Lifetime
- 1989-09-19 DE DE68924750T patent/DE68924750T2/de not_active Expired - Fee Related
- 1989-09-19 EP EP89309532A patent/EP0361768B1/de not_active Expired - Lifetime
- 1989-09-19 AT AT89309532T patent/ATE130046T1/de not_active IP Right Cessation
- 1989-09-26 JP JP1250374A patent/JPH02257897A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
ATE130046T1 (de) | 1995-11-15 |
EP0361768A3 (de) | 1991-06-05 |
EP0361768B1 (de) | 1995-11-08 |
JPH02257897A (ja) | 1990-10-18 |
DE68924750D1 (de) | 1995-12-14 |
CA1333366C (en) | 1994-12-06 |
ES2081848T3 (es) | 1996-03-16 |
EP0670329A2 (de) | 1995-09-06 |
EP0361768A2 (de) | 1990-04-04 |
EP0670329A3 (de) | 1995-12-06 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8339 | Ceased/non-payment of the annual fee |