ATE115959T1 - Markiertes dna. - Google Patents

Markiertes dna.

Info

Publication number
ATE115959T1
ATE115959T1 AT84305989T AT84305989T ATE115959T1 AT E115959 T1 ATE115959 T1 AT E115959T1 AT 84305989 T AT84305989 T AT 84305989T AT 84305989 T AT84305989 T AT 84305989T AT E115959 T1 ATE115959 T1 AT E115959T1
Authority
AT
Austria
Prior art keywords
labels
dna molecule
bonded
dna
marked dna
Prior art date
Application number
AT84305989T
Other languages
English (en)
Inventor
Greg M Landes
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genzyme Corp filed Critical Genzyme Corp
Application granted granted Critical
Publication of ATE115959T1 publication Critical patent/ATE115959T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Lubrication Of Internal Combustion Engines (AREA)
AT84305989T 1983-09-02 1984-08-31 Markiertes dna. ATE115959T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/529,044 US4626501A (en) 1983-09-02 1983-09-02 Labeled DNA

Publications (1)

Publication Number Publication Date
ATE115959T1 true ATE115959T1 (de) 1995-01-15

Family

ID=24108275

Family Applications (1)

Application Number Title Priority Date Filing Date
AT84305989T ATE115959T1 (de) 1983-09-02 1984-08-31 Markiertes dna.

Country Status (6)

Country Link
US (1) US4626501A (de)
EP (1) EP0138357B1 (de)
JP (1) JPS60172994A (de)
AT (1) ATE115959T1 (de)
CA (1) CA1234061A (de)
DE (1) DE3486362T2 (de)

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5200313A (en) * 1983-08-05 1993-04-06 Miles Inc. Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
US4707440A (en) * 1984-01-30 1987-11-17 Enzo Biochem, Inc. Nucleic acid hybridization assay and detectable molecules useful in such assay
US4952685A (en) * 1984-01-30 1990-08-28 Enzo Biochem, Inc. Detectable molecules, method of preparation and use
US5002885A (en) * 1984-01-30 1991-03-26 Enzo Biochem, Inc. Detectable molecules, method preparation and use
US4957858A (en) * 1986-04-16 1990-09-18 The Salk Instute For Biological Studies Replicative RNA reporter systems
DE3431536A1 (de) * 1984-08-28 1986-03-13 Boehringer Mannheim Gmbh, 6800 Mannheim Derivatisierte nucleinsaeure-sequenz, verfahren zu deren herstellung sowie deren verwendung zum nachweis von nucleinsaeuren
US4828979A (en) * 1984-11-08 1989-05-09 Life Technologies, Inc. Nucleotide analogs for nucleic acid labeling and detection
US4824775A (en) * 1985-01-03 1989-04-25 Molecular Diagnostics, Inc. Cells labeled with multiple Fluorophores bound to a nucleic acid carrier
EP0187332B1 (de) * 1985-01-10 1989-02-01 Molecular Diagnostics, Inc. Photochemisches Verfahren zum Markieren von Nucleinsäuren zum Nachweis in Hybridisationsproben
US5026840A (en) * 1985-01-10 1991-06-25 Molecular Diagnostics, Inc. Photochemical nucleic acid-labeling reagent having a polyalklamine spacer
DE3679690D1 (de) * 1985-03-19 1991-07-18 Abbott Lab Diagnostische probe, die mikroperoxidase verwendet.
GB8509880D0 (en) * 1985-04-17 1985-05-22 Ici Plc Testing device
JPH0723393B2 (ja) * 1985-05-15 1995-03-15 アモコ・コーポレーション シチジン同族体
EP0204510B1 (de) * 1985-05-31 1991-07-10 Amoco Corporation Verstärkung von Hybridisierungssignalen durch Verwendung von komplementarischen DNS-Strängen
US4833251A (en) * 1985-06-25 1989-05-23 Siska Diagnostics, Inc. Compounds for tagging nucleic acid probes
US5093232A (en) * 1985-12-11 1992-03-03 Chiron Corporation Nucleic acid probes
US4882269A (en) * 1985-12-13 1989-11-21 Princeton University Amplified hybridization assay
DE3785591T2 (de) * 1986-01-10 1993-09-02 Amoco Corp Kompetitiver homogener test.
US4925785A (en) * 1986-03-07 1990-05-15 Biotechnica Diagnostics, Inc. Nucleic acid hybridization assays
GB8621337D0 (en) * 1986-09-04 1986-10-15 Agricultural Genetics Co Non-radioactive nucleic acid hybridization probes
US5428163A (en) * 1986-12-31 1995-06-27 Mills; Randell L. Prodrugs for selective drug delivery
IL86164A0 (en) * 1987-04-28 1988-11-15 Tamir Biotechnology Ltd Improved dna probes
FR2618160B1 (fr) * 1987-07-16 1990-03-09 Ire Celltarg Sa Technique enzymatique utilisant un chromogene lyophilise, trousse de reactif et procede de lyophilisation
FR2620122B1 (fr) * 1987-09-03 1989-12-29 Pasteur Institut Procede d'obtention par voie chimique d'oligonucleotides marques et applications biologiques
US4962029A (en) * 1987-10-02 1990-10-09 Cetus Corporation Covalent oligonucleotide-horseradish peroxidase conjugate
JPH0630574B2 (ja) * 1987-10-02 1994-04-27 シタス コーポレイション オリゴヌクレオチド‐西洋ワサビパーオキシダーゼ共有結合接合体
US5656731A (en) * 1987-10-15 1997-08-12 Chiron Corporation Nucleic acid-amplified immunoassay probes
CA1341077C (en) * 1988-03-31 2000-08-08 Randell L. Mills Luminide and macroluminide class of pharmaceuticals
US6326136B1 (en) 1988-04-01 2001-12-04 Digene Corporation Macromolecular conjugate made using unsaturated aldehydes
US5109124A (en) * 1988-06-01 1992-04-28 Biogen, Inc. Nucleic acid probe linked to a label having a terminal cysteine
FR2644163B2 (fr) * 1988-07-13 1994-09-30 Bioprobe Systems Sa Procede de marquage d'une sonde nucleique et trousse de reactifs pour la mise en oeuvre de ce procede
FR2634211B1 (fr) * 1988-07-13 1992-06-26 Lebacq Philippe Procede de marquage d'une sonde nucleique et trousse de reactifs pour la mise en oeuvre de ce procede
US5858652A (en) * 1988-08-30 1999-01-12 Abbott Laboratories Detection and amplification of target nucleic acid sequences
US5082935A (en) * 1988-12-15 1992-01-21 Amoco Corporation Diagnostic reagents made by attaching cytidine containing nucleic acid probes to amino functionalized solid supports by bisulfite mediated transamination
US5049489A (en) * 1989-04-17 1991-09-17 The Standard Oil Company 16S rRNA oligonucleotide probes for the identification of sulfate-reducing bacteria
WO1991002040A1 (en) * 1989-08-04 1991-02-21 Kosak Kenneth M Cyclodextrin labels for nucleic acid and biochemical analysis
GB8927503D0 (en) * 1989-12-04 1990-02-07 Kronem Systems Inc Enzyme-amplified lanthanide chelate luminescence
US5849481A (en) * 1990-07-27 1998-12-15 Chiron Corporation Nucleic acid hybridization assays employing large comb-type branched polynucleotides
RU2142467C1 (ru) * 1990-07-27 1999-12-10 Чирон Диагностикс Корпорейшн Полинуклеотиды, способ их получения, гибридизационный анализ нуклеиновой кислоты
US5258507A (en) * 1990-11-08 1993-11-02 Amoco Corporation Labeling reagents useful for the chemical attachment of nitrophenyl derivative ligands to DNA probes
US5512433A (en) * 1990-11-08 1996-04-30 Vysis, Inc. Methods and compounds for labeling DNA with xanthine and lower alkyl substituted xanthine derivatives and reagents for the in situ detection of chromosomes
ZA936015B (en) * 1992-08-24 1994-03-10 Akzo Nv Elimination of false negatives in nuleic acid detection.
JP3612534B2 (ja) * 1998-09-04 2005-01-19 独立行政法人理化学研究所 Ｄｎａ固定支持方法およびｄｎａ固定支持体
AU2001266861A1 (en) * 2000-06-12 2001-12-24 Randell L. Mills Photocleavable prodrugs for selective drug delivery
GB0029154D0 (en) * 2000-11-30 2001-01-17 Lee Helen Signal enhancement with multiple labelled-antibodies
US7361310B1 (en) * 2001-11-30 2008-04-22 Northwestern University Direct write nanolithographic deposition of nucleic acids from nanoscopic tips
US20050080260A1 (en) * 2003-04-22 2005-04-14 Mills Randell L. Preparation of prodrugs for selective drug delivery
AU2008266889A1 (en) * 2007-06-20 2008-12-24 Northwestern University Patterning with compositions comprising lipid
JP2010536033A (ja) * 2007-08-08 2010-11-25 ノースウエスタン ユニバーシティ カンチレバーアレイのための、独立してアドレス可能な自己修正インク付け方法
KR101935146B1 (ko) * 2017-04-26 2019-01-03 아주대학교산학협력단 재귀반사 현상을 이용한 분자 비콘 기반의 광학 유전자 바이오센서 및 이를 이용한 핵산 분자의 정량 분석 방법
GB201912866D0 (en) * 2019-09-06 2019-10-23 Cambridge Molecular Diagnostics Ltd Miltiply labelled polymetric molecule for detection assays

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4139346A (en) * 1977-11-28 1979-02-13 Enzo Bio Chem Incorporated Nucleic acid and protein binding paper
FR2422956A1 (fr) * 1978-04-13 1979-11-09 Pasteur Institut Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede
US4228237A (en) * 1978-09-21 1980-10-14 Calbiochem-Behring Corp. Methods for the detection and determination of ligands
US4286964A (en) * 1979-10-12 1981-09-01 Seed Brian S Polyfunctional epoxides and halohydrins used as bridging groups to bind aromatic amine group-containing alcohols and thiols to hydroxyl bearing substrates
US4282287A (en) * 1980-01-24 1981-08-04 Giese Roger W Biochemical avidin-biotin multiple-layer system
US4358535A (en) * 1980-12-08 1982-11-09 Board Of Regents Of The University Of Washington Specific DNA probes in diagnostic microbiology
FR2519005B1 (fr) * 1981-12-29 1985-10-25 Pasteur Institut Fragments d'adn marques a l'une au moins de leurs extremites par des ribonucleotides modifies reconnaissables par des molecules affines et procede pour realiser une analyse de tels fragments d'adn

Also Published As

Publication number Publication date
CA1234061A (en) 1988-03-15
EP0138357B1 (de) 1994-12-21
DE3486362D1 (de) 1995-02-02
US4626501A (en) 1986-12-02
EP0138357A3 (en) 1986-05-07
JPS60172994A (ja) 1985-09-06
DE3486362T2 (de) 1995-05-11
EP0138357A2 (de) 1985-04-24

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