ES2074087T3 - Peptido-señal y secuencias de adn que codifican para el mismo. - Google Patents
Peptido-señal y secuencias de adn que codifican para el mismo.Info
- Publication number
- ES2074087T3 ES2074087T3 ES89402328T ES89402328T ES2074087T3 ES 2074087 T3 ES2074087 T3 ES 2074087T3 ES 89402328 T ES89402328 T ES 89402328T ES 89402328 T ES89402328 T ES 89402328T ES 2074087 T3 ES2074087 T3 ES 2074087T3
- Authority
- ES
- Spain
- Prior art keywords
- amino acids
- peptide
- dna sequences
- sequences coding
- signal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108091028043 Nucleic acid sequence Proteins 0.000 title abstract 2
- 108010076504 Protein Sorting Signals Proteins 0.000 title abstract 2
- 235000001014 amino acid Nutrition 0.000 abstract 4
- 229940024606 amino acid Drugs 0.000 abstract 4
- 150000001413 amino acids Chemical class 0.000 abstract 4
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 3
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 abstract 2
- 230000002068 genetic effect Effects 0.000 abstract 2
- 238000004519 manufacturing process Methods 0.000 abstract 2
- 229920001184 polypeptide Polymers 0.000 abstract 2
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 2
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 abstract 1
- 239000004471 Glycine Substances 0.000 abstract 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 abstract 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 abstract 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 abstract 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 abstract 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 abstract 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 abstract 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 abstract 1
- 239000004472 Lysine Substances 0.000 abstract 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 abstract 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 abstract 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 abstract 1
- 239000004473 Threonine Substances 0.000 abstract 1
- 235000004279 alanine Nutrition 0.000 abstract 1
- 235000009582 asparagine Nutrition 0.000 abstract 1
- 229960001230 asparagine Drugs 0.000 abstract 1
- 235000018417 cysteine Nutrition 0.000 abstract 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 abstract 1
- 229930182817 methionine Natural products 0.000 abstract 1
- 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 abstract 1
- 125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Endocrinology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Saccharide Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
LA INVENCION SE REFIERE A UN PEPTIDO-SEÑAL DE FORMULA MXKSTLLLLFLLLCLPSWNAGA, DONDE A=ALANINA, C=CISTEINA, F=FENILALANINA, G=GLICINA, K=LISINA, L=LEUCINA, M=METIONINA, N=ASPARRAGINA, P=PROLINA, S=SERINA, T=TEONINA, W=TRIPTOFANO, Y X REPRESENTA UNA UNION DIRECTA ENTRE M Y K, UN AMINOACIDO ELEGIDO DEL GRUPO DE 20 AMINOACIDOS, DEL CODIGO GENETICO O UN PEPTIDO QUE TIENE 2, 3 O 4 AMINOACIDOS ELEGIDOS CADA UNO INDEPENDIENTEMENTE EN EL CONJUNTO DE 20 AMINOACIDOS DEL CODIGO GENETICO. SE REFIERE IGUALMENTE A LAS SECUENCIAS DE ADN QUE CODIFICAN PARA EL, A LOS VECTORES DE EXPRESION QUE LLEVAN UNA DE ESTAS SECUENCIAS, A LAS BACTERIAS GAM-NEGATIVAS TRANSFORMADAS POR ESTOS VECTORES Y AL PROCEDIMIENTO DE PRODUCCION PERIPLASMICA DE UN POLIPEPTIDO. APLICACION: FABRICACION DE POLIPEPTIDOS, TAL COMO HGH O HIRUDINA.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR8811188A FR2636643B1 (fr) | 1988-08-24 | 1988-08-24 | Peptide-signal, sequences d'adn codant pour celui-ci, vecteurs d'expression portant l'une de ces sequences et procede de production periplasmique d'un polypeptide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2074087T3 true ES2074087T3 (es) | 1995-09-01 |
Family
ID=9369498
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES89402328T Expired - Lifetime ES2074087T3 (es) | 1988-08-24 | 1989-08-23 | Peptido-señal y secuencias de adn que codifican para el mismo. |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP0356335B1 (es) |
| JP (1) | JP2535076B2 (es) |
| AT (1) | ATE122398T1 (es) |
| CA (1) | CA1334944C (es) |
| DE (1) | DE68922549T2 (es) |
| ES (1) | ES2074087T3 (es) |
| FR (1) | FR2636643B1 (es) |
| GR (1) | GR3016981T3 (es) |
| PT (1) | PT91511B (es) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4009268A1 (de) * | 1990-03-22 | 1991-09-26 | Consortium Elektrochem Ind | Sekretion von hirudinderivaten |
| US6514730B1 (en) | 1991-03-21 | 2003-02-04 | Consortium für elektrochemische Industrie GmbH | Secretion of hirudin derivatives |
| WO2001094418A2 (en) * | 2000-06-05 | 2001-12-13 | Corixa Corporation | Leader peptides for enhancing secretion of recombinant protein from a host cell |
| FR2957598B1 (fr) | 2010-03-17 | 2015-12-04 | Lfb Biotechnologies | Nouveau peptide signal, et son utilisation pour la production de proteines recombinantes |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3587205T2 (de) * | 1984-07-30 | 1993-08-26 | Wakunaga Seiyaku Kk | Verfahren zur herstellung eines proteins und dafuer zu verwendender vektor, rekombinante dns und transformierte zelle. |
| DE3586386T2 (de) * | 1984-10-05 | 1993-01-14 | Genentech Inc | Dna, zellkulturen und verfahren zur sekretion von heterologen proteinen und periplasmische proteinrueckgewinnung. |
-
1988
- 1988-08-24 FR FR8811188A patent/FR2636643B1/fr not_active Expired - Lifetime
-
1989
- 1989-08-22 PT PT91511A patent/PT91511B/pt not_active IP Right Cessation
- 1989-08-23 CA CA000609139A patent/CA1334944C/en not_active Expired - Lifetime
- 1989-08-23 AT AT89402328T patent/ATE122398T1/de not_active IP Right Cessation
- 1989-08-23 ES ES89402328T patent/ES2074087T3/es not_active Expired - Lifetime
- 1989-08-23 DE DE68922549T patent/DE68922549T2/de not_active Expired - Lifetime
- 1989-08-23 EP EP89402328A patent/EP0356335B1/fr not_active Expired - Lifetime
- 1989-08-24 JP JP1220106A patent/JP2535076B2/ja not_active Expired - Lifetime
-
1995
- 1995-08-02 GR GR950402097T patent/GR3016981T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ATE122398T1 (de) | 1995-05-15 |
| EP0356335B1 (fr) | 1995-05-10 |
| FR2636643B1 (fr) | 1990-12-28 |
| DE68922549D1 (de) | 1995-06-14 |
| GR3016981T3 (en) | 1995-11-30 |
| JP2535076B2 (ja) | 1996-09-18 |
| JPH02257883A (ja) | 1990-10-18 |
| PT91511B (pt) | 1995-07-18 |
| EP0356335A1 (fr) | 1990-02-28 |
| DE68922549T2 (de) | 1996-01-18 |
| PT91511A (pt) | 1990-03-08 |
| FR2636643A1 (fr) | 1990-03-23 |
| CA1334944C (en) | 1995-03-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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