ES2070917T3 - Ensayo a base de cuantificacion de un enzima sobre una mecha. - Google Patents
Ensayo a base de cuantificacion de un enzima sobre una mecha.Info
- Publication number
- ES2070917T3 ES2070917T3 ES89313626T ES89313626T ES2070917T3 ES 2070917 T3 ES2070917 T3 ES 2070917T3 ES 89313626 T ES89313626 T ES 89313626T ES 89313626 T ES89313626 T ES 89313626T ES 2070917 T3 ES2070917 T3 ES 2070917T3
- Authority
- ES
- Spain
- Prior art keywords
- analyte
- enzyme
- fragment
- complementary
- support
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
LOS ENSAYOS DIAGNOSTICOS SE PROPORCIONAN COMPRENDIENDO FRAGMENTOS DE ENZIMA COMPLEMENTARIA DE BETA-GALACTOSIDASA, DONDE UNO DE LOS FRAGMENTOS SE LIUMITA A UN APOYO Y EL OTRO FRAGMENTO SE CONJUGA A UN EPITOPO REACTIVO CONTRARIO DEL ANALITICO O COMPLEMENTARIO PARA UN RECEPTOR DE ANALITICO. UNO PUEDE LOGRAR UNA DETERMINACION DE LIGANDO, PROPORCIONANDO LA COMPETENCIA ENTRE EL ANALITICO DE LIGANDO Y EL CONJUGADO PARA RECEPTOR, EN EL QUE LA UNION AL RECEPTOR PERMITE LA DISCRIMINACION ENTRE CONJUGADO DE FRAGMENTO DE ENZIMAS COMPUESTO Y EL CONJUGADO DE FRAGMENTO DE ENZIMA NO COMPUESTO. EL MEDIO DE ENSAYO ES LUEGO PERMITIDO PARA EMPAQUETAR EN UN SOPORTE AL QUE EL FRAGMENTO COMPLEMENTARIO ESTA UNIDO SUSTANCIALMENTE DE MANERA UNIFORME EN LA REGION DE DETECCION. EL SOPORTE ES LUEGO REVELADO, DONDE LA FORMACION DE COLOR DEL SUBSTRATO EN LA REGION DE DETECCION SE UTILIZA COMO UNA MEDIDA DE LA PRESENCIA DEL ANALITICO EN LA MUESTRA.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US29145788A | 1988-12-29 | 1988-12-29 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2070917T3 true ES2070917T3 (es) | 1995-06-16 |
Family
ID=23120370
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES89313626T Expired - Lifetime ES2070917T3 (es) | 1988-12-29 | 1989-12-28 | Ensayo a base de cuantificacion de un enzima sobre una mecha. |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP0376707B1 (es) |
JP (1) | JP2667718B2 (es) |
AT (1) | ATE118617T1 (es) |
AU (1) | AU615780B2 (es) |
CA (1) | CA1336163C (es) |
DE (1) | DE68921184T2 (es) |
ES (1) | ES2070917T3 (es) |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4708929A (en) * | 1984-10-29 | 1987-11-24 | Microgenics Corporation | Methods for protein binding enzyme complementation assays |
AU5280086A (en) * | 1985-01-30 | 1986-08-07 | Genetic Diagnostics Corp. | Self timed immunoassay device |
EP0271204A3 (en) * | 1986-11-07 | 1989-10-25 | Syntex (U.S.A.) Inc. | Immunochromatographic method and device |
WO1989002597A1 (en) * | 1987-09-21 | 1989-03-23 | Microgenics Corporation | Solid-phase non-separation enzyme assay |
-
1989
- 1989-08-15 AU AU39930/89A patent/AU615780B2/en not_active Ceased
- 1989-09-26 CA CA000613155A patent/CA1336163C/en not_active Expired - Fee Related
- 1989-11-02 JP JP1285117A patent/JP2667718B2/ja not_active Expired - Fee Related
- 1989-12-28 DE DE68921184T patent/DE68921184T2/de not_active Expired - Fee Related
- 1989-12-28 EP EP89313626A patent/EP0376707B1/en not_active Expired - Lifetime
- 1989-12-28 AT AT89313626T patent/ATE118617T1/de not_active IP Right Cessation
- 1989-12-28 ES ES89313626T patent/ES2070917T3/es not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
AU615780B2 (en) | 1991-10-10 |
JPH02201265A (ja) | 1990-08-09 |
JP2667718B2 (ja) | 1997-10-27 |
EP0376707A3 (en) | 1990-10-31 |
ATE118617T1 (de) | 1995-03-15 |
AU3993089A (en) | 1990-07-05 |
DE68921184T2 (de) | 1995-08-31 |
DE68921184D1 (de) | 1995-03-23 |
EP0376707A2 (en) | 1990-07-04 |
CA1336163C (en) | 1995-07-04 |
EP0376707B1 (en) | 1995-02-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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