ES2060580T3 - Adn que tiene una secuencia adn codificante de la proteina flagelina y vector que la contiene. - Google Patents

Adn que tiene una secuencia adn codificante de la proteina flagelina y vector que la contiene.

Info

Publication number
ES2060580T3
ES2060580T3 ES87103488T ES87103488T ES2060580T3 ES 2060580 T3 ES2060580 T3 ES 2060580T3 ES 87103488 T ES87103488 T ES 87103488T ES 87103488 T ES87103488 T ES 87103488T ES 2060580 T3 ES2060580 T3 ES 2060580T3
Authority
ES
Spain
Prior art keywords
dna
flageline
flagels
strange
vector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES87103488T
Other languages
English (en)
Inventor
Junichiro Asaka
Tamio Fujiwara
Takashi Fujiwara
Goro Kuwajima
Eiji Kondo
Masaru Shin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shionogi and Co Ltd
Original Assignee
Shionogi and Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP61223484A external-priority patent/JP2584213B2/ja
Application filed by Shionogi and Co Ltd filed Critical Shionogi and Co Ltd
Application granted granted Critical
Publication of ES2060580T3 publication Critical patent/ES2060580T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00—Reverse transcribing DNA viruses
    • C12N2730/00011—Details
    • C12N2730/10011—Hepadnaviridae
    • C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

SE DETERMINA LA SECUENCIA COMPLETA DE ADN QUE CODIFICA LA FLAGELINA DE E.COLI (GEN HAG) CLONIFICADO EN EL FAGO LAMBDA. EL GEN HAG SE INTRODUCE EN PBR322 Y FALTA LA REGION DE GEN HAG CONCERNIENTE A LA ANTIGENICIDAD DE LOS FLAGELOS EN LA QUE SE INSERTAN LOS ESLABONES. CUANDO EL VECTOR QUE TIENE EL ADN EXTRAÑO INSERTADO EN EL ESLABON SE INTRODUCE EN EL E.COLI QUE NO FORMA FLAGELOS, EL E.COLI SE HACE CAPAZ DE FORMAR FLAGELOS Y MOSTRAR MOTILIDAD. LOS FLAGELOS CONSISTEN EN FLAGELINA CONDENSADA CON EL PEPTIDO EXTRAÑO CODIFICADO POR EL ADN EXTRAÑO. ESPECIALMENTE EL PEPTIDO EXTRAÑO SE EXCRETA FUERA DE LAS BACTERIAS. ESTE SISTEMA PUDE SER UTILIZADO EN LA DETERMINACION DEL EPITOPE Y EN LA PREPARACION DE ANTIGENO ASI COMO EN LA EXCRECION DEL PEPTIDO.
ES87103488T 1986-03-11 1987-03-11 Adn que tiene una secuencia adn codificante de la proteina flagelina y vector que la contiene. Expired - Lifetime ES2060580T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP5440086 1986-03-11
JP61223484A JP2584213B2 (ja) 1986-03-11 1986-09-19 フラジエリンをコ−ドするｄｎａおよび該ｄｎａを有するベクタ−

Publications (1)

Publication Number Publication Date
ES2060580T3 true ES2060580T3 (es) 1994-12-01

Family

ID=26395161

Family Applications (1)

Application Number Title Priority Date Filing Date
ES87103488T Expired - Lifetime ES2060580T3 (es) 1986-03-11 1987-03-11 Adn que tiene una secuencia adn codificante de la proteina flagelina y vector que la contiene.

Country Status (4)

Country Link
US (1) US4886748A (es)
EP (1) EP0237045B1 (es)
DE (1) DE3787820T2 (es)
ES (1) ES2060580T3 (es)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0241546A4 (en) * 1985-10-11 1988-11-02 Genetics Inst METHOD FOR PRODUCING HETEROLOGICAL PROTEINS.
US4801536A (en) * 1985-10-11 1989-01-31 Genetics Institute, Inc. Method for producing heterologous proteins
EP0419513B1 (en) * 1988-05-05 1995-04-26 American Cyanamid Company Recombinant flagellin vaccines
US6130082A (en) * 1988-05-05 2000-10-10 American Cyanamid Company Recombinant flagellin vaccines
DK0413378T3 (da) * 1989-08-18 1995-05-15 Akzo Nobel Nv Escherichia coli vaccine
US5635182A (en) * 1994-06-16 1997-06-03 Genetics Institute, Inc. Method of detecting ligand interactions
DE60011560T2 (de) * 1999-12-28 2005-08-18 Akzo Nobel N.V. Salmonella Impfstoff, der keinen Antikörper gegen Flagellin oder gegen Flagella induziert
US20030232055A1 (en) * 2000-07-31 2003-12-18 Ruslan Medzhitov Innate immune system-directed vaccines
US20030175287A1 (en) * 2001-01-03 2003-09-18 Yale University Innate immune system-directed vaccines
CN101128480A (zh) 2004-12-02 2008-02-20 Csir公司 含有缺损的鞭毛蛋白基因的革兰氏阳性菌细胞、基于鞭毛蛋白的融合蛋白以及从液体中除去金属离子的用途
JP5094407B2 (ja) * 2004-12-16 2012-12-12 ウェイク・フォレスト・ユニヴァーシティ・ヘルス・サイエンシズ Ｙｅｒｓｉｎｉａｐｅｓｔｉｓの免疫療法におけるフラジェリンの使用
EP2476432B1 (en) 2006-03-07 2015-08-19 Vaxinnate Corporation Compositions that include hemagglutinin, methods of making and methods of use thereof
US8020260B2 (en) * 2007-04-16 2011-09-20 Formetco, Inc. Locking clip for a billboard sign tensioning system
CA2739454C (en) * 2008-10-03 2018-05-01 Emory University Methods for the treatment of graft-versus-host disease
WO2010107778A1 (en) 2009-03-18 2010-09-23 Wake Forest University Health Sciences Flagellin fusion proteins and use thereof to induce immune responses against pseudomonas aeruginosa
WO2010141312A2 (en) 2009-06-01 2010-12-09 Wake Forest University Health Sciences Flagellin fusion proteins and conjugates comprising pneumococcus antigens and methods of using the same
US20120171239A1 (en) 2009-09-03 2012-07-05 Mizel Steven B Immunogenic Conjugates for Producing Immune Responses to Drugs of Abuse and Methods of Use
ES2690760T3 (es) 2010-06-25 2018-11-22 Inserm - Institut National De La Santé Et De La Recherche Médicale Polipéptido de flagelina como agonista de TLR5 para su uso en el tratamiento de infecciones de las vías respiratorias
EP3632458A1 (en) 2013-07-26 2020-04-08 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods and pharmaceutical compositions for the treatment of bacterial infections
KR102524577B1 (ko) 2019-04-22 2023-04-21 전남대학교산학협력단 플라젤린 융합 단백질 및 이의 용도
FR3114970B1 (fr) 2020-10-08 2023-06-30 Univ Tours Combinaison d’anticorps inhalés avec des agents immunomodulateurs pour le traitement ou la prévention d’inféctions respiratoires
KR102461645B1 (ko) * 2020-10-20 2022-11-01 전남대학교산학협력단 플라젤린 융합 단백질을 포함하는 비알코올성 지방간질환 또는 대사증후군 예방 또는 치료용 조성물
WO2023275292A2 (en) 2021-06-30 2023-01-05 INSERM (Institut National de la Santé et de la Recherche Médicale) Aerosol composition for pulmonary delivery of flagellin

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0241546A4 (en) * 1985-10-11 1988-11-02 Genetics Inst METHOD FOR PRODUCING HETEROLOGICAL PROTEINS.

Also Published As

Publication number Publication date
EP0237045B1 (en) 1993-10-20
DE3787820T2 (de) 1994-04-28
US4886748A (en) 1989-12-12
EP0237045A2 (en) 1987-09-16
DE3787820D1 (de) 1993-11-25
EP0237045A3 (en) 1988-11-02

Similar Documents

Publication Publication Date Title
ES2060580T3 (es) Adn que tiene una secuencia adn codificante de la proteina flagelina y vector que la contiene.
MX6339E (es) Metodo para elaborar dentro de un huesped bacteriano y excretar a traves de la membrana de la celula huesped,una proteina o un polipeptido seleccionados
ES2127829T3 (es) Expresion de proteinas recombinantes fusionadas en bacterias atenuadas.
CY1113218T1 (el) Υβριδιο και διμερης εκφραση πρωτεϊνων ναϊσσεριας
ES2077666T3 (es) Proteina de union a fibronectina y su preparacion.
PT93910A (pt) Prodesso para a preparacao de uma proteina heterologa mediante secrecao a partir de uma celula
ATE118352T1 (de) Erreger der mysteriösen schweinekrankheit,impfstoff-zusammensetzungen und diagnose kits.
DE69523052T2 (de) Gereinigte myceliophthora laccasen und nukleinsäuren dafür kodierend
ES2153037T3 (es) Lacasas purificadas de scytalidium y acidos nucleicos que las codifican.
DE69926647D1 (de) Durch molekulares pathogenicid vermittelte krankheitsresistenz in pflanzen
BR1100650A (pt) Proteìnas de superfìcie cd44 variantes, sequências de dna que as codificam, anticorpos contra estas proteìnas, bem como a sua utilização no diagnóstico e na terapia
AR246765A1 (es) Novedosos genes y secuencias de adn del factor i del crecimiento del tipo de la preproinsulina humana.
KR890000517A (ko) 하이브리드 단백질 c 및 이의 제조방법
FR2649982B1 (fr) Membrane biologique artificielle
DK610888D0 (da) Cardiodilatinfragmentet, fremgangsmaade til fremstilling deraf samt dets anvendelse
NO930428D0 (no) Proteinstrukturen til plantetoksingelonin
DE3881023D1 (de) Proteine, expressionsvektoren, die fuer solche proteine kodierende dna enthalten und zellen, die solche expressionsvektoren enthalten.
ATE432287T1 (de) Peptidfragmente mit den zelltod verhindernder aktivität
ES2090357T3 (es) Una proteina de union a colageno asi como su preparacion.
KR910003095A (ko) 사람의 단백질 c변이체 및 이의 제조방법
KR890006253A (ko) 가금류내의 E.coli septicaemia에 대한 백신
ES2074179T3 (es) Haptenos marcados mejorados, procedimiento para su preparacion, asi como empleo de estos haptenos marcados en inmunoensayos.
DE3886443T2 (de) Menschliche Rhinoviruspeptide.
ES2063223T5 (es) Anticuerpos contra secuencias de aminoacidos muy conservadas de sustancias inmunogenas, procedimiento para la preparacion de estos anticuerpos, asi como su utilizacion en inmunoensayos.
DK0938555T3 (da) Konstruktioner og komplekser af cyclin E

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 237045

Country of ref document: ES