ES2060580T3 - Adn que tiene una secuencia adn codificante de la proteina flagelina y vector que la contiene. - Google Patents
Adn que tiene una secuencia adn codificante de la proteina flagelina y vector que la contiene.Info
- Publication number
- ES2060580T3 ES2060580T3 ES87103488T ES87103488T ES2060580T3 ES 2060580 T3 ES2060580 T3 ES 2060580T3 ES 87103488 T ES87103488 T ES 87103488T ES 87103488 T ES87103488 T ES 87103488T ES 2060580 T3 ES2060580 T3 ES 2060580T3
- Authority
- ES
- Spain
- Prior art keywords
- dna
- flageline
- flagels
- strange
- vector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2730/00—Reverse transcribing DNA viruses
- C12N2730/00011—Details
- C12N2730/10011—Hepadnaviridae
- C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
- C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Virology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Saccharide Compounds (AREA)
Abstract
SE DETERMINA LA SECUENCIA COMPLETA DE ADN QUE CODIFICA LA FLAGELINA DE E.COLI (GEN HAG) CLONIFICADO EN EL FAGO LAMBDA. EL GEN HAG SE INTRODUCE EN PBR322 Y FALTA LA REGION DE GEN HAG CONCERNIENTE A LA ANTIGENICIDAD DE LOS FLAGELOS EN LA QUE SE INSERTAN LOS ESLABONES. CUANDO EL VECTOR QUE TIENE EL ADN EXTRAÑO INSERTADO EN EL ESLABON SE INTRODUCE EN EL E.COLI QUE NO FORMA FLAGELOS, EL E.COLI SE HACE CAPAZ DE FORMAR FLAGELOS Y MOSTRAR MOTILIDAD. LOS FLAGELOS CONSISTEN EN FLAGELINA CONDENSADA CON EL PEPTIDO EXTRAÑO CODIFICADO POR EL ADN EXTRAÑO. ESPECIALMENTE EL PEPTIDO EXTRAÑO SE EXCRETA FUERA DE LAS BACTERIAS. ESTE SISTEMA PUDE SER UTILIZADO EN LA DETERMINACION DEL EPITOPE Y EN LA PREPARACION DE ANTIGENO ASI COMO EN LA EXCRECION DEL PEPTIDO.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP5440086 | 1986-03-11 | ||
JP61223484A JP2584213B2 (ja) | 1986-03-11 | 1986-09-19 | フラジエリンをコ−ドするdnaおよび該dnaを有するベクタ− |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2060580T3 true ES2060580T3 (es) | 1994-12-01 |
Family
ID=26395161
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES87103488T Expired - Lifetime ES2060580T3 (es) | 1986-03-11 | 1987-03-11 | Adn que tiene una secuencia adn codificante de la proteina flagelina y vector que la contiene. |
Country Status (4)
Country | Link |
---|---|
US (1) | US4886748A (es) |
EP (1) | EP0237045B1 (es) |
DE (1) | DE3787820T2 (es) |
ES (1) | ES2060580T3 (es) |
Families Citing this family (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4801536A (en) * | 1985-10-11 | 1989-01-31 | Genetics Institute, Inc. | Method for producing heterologous proteins |
EP0241546A4 (en) * | 1985-10-11 | 1988-11-02 | Genetics Inst | METHOD FOR PRODUCING HETEROLOGICAL PROTEINS. |
WO1989010967A1 (en) * | 1988-05-05 | 1989-11-16 | Praxis Biologics, Inc. | Recombinant flagellin vaccines |
US6130082A (en) * | 1988-05-05 | 2000-10-10 | American Cyanamid Company | Recombinant flagellin vaccines |
EP0413378B1 (en) * | 1989-08-18 | 1995-01-18 | Akzo Nobel N.V. | Escherichia coli vaccine |
US5635182A (en) * | 1994-06-16 | 1997-06-03 | Genetics Institute, Inc. | Method of detecting ligand interactions |
EP1112747B1 (en) * | 1999-12-28 | 2004-06-16 | Akzo Nobel N.V. | Salmonella vaccine not inducing antibodies against flagellin or flagella |
US20030232055A1 (en) * | 2000-07-31 | 2003-12-18 | Ruslan Medzhitov | Innate immune system-directed vaccines |
US20030175287A1 (en) * | 2001-01-03 | 2003-09-18 | Yale University | Innate immune system-directed vaccines |
AU2005323811B2 (en) * | 2004-12-02 | 2012-07-05 | Csir | Gram positive bacterial cells comprising a disrupted flagellin gene, flagellin-based fusion proteins and use in removal of metal ions from a liquid |
WO2006066214A2 (en) * | 2004-12-16 | 2006-06-22 | Wake Forest University Health Sciences | Use of flagellin in the immunotherapy of yersinia pestis |
AU2007224034B2 (en) | 2006-03-07 | 2012-03-29 | Vaxinnate Corporation | Compositions that include hemagglutinin, methods of making and methods of use thereof |
US8020260B2 (en) * | 2007-04-16 | 2011-09-20 | Formetco, Inc. | Locking clip for a billboard sign tensioning system |
CA2739454C (en) * | 2008-10-03 | 2018-05-01 | Emory University | Methods for the treatment of graft-versus-host disease |
US9096659B2 (en) | 2009-03-18 | 2015-08-04 | Wake Forest University Health Sciences | Flagellin fusion proteins and use thereof to induce immune responses against Pseudomonas aeruginosa |
US20120135037A1 (en) | 2009-06-01 | 2012-05-31 | Mizel Steven B | Flagellin fusion proteins and conjugates comprising pneumococcus antigens and methods of using the same |
US20120171239A1 (en) | 2009-09-03 | 2012-07-05 | Mizel Steven B | Immunogenic Conjugates for Producing Immune Responses to Drugs of Abuse and Methods of Use |
JP5746761B2 (ja) | 2010-06-25 | 2015-07-08 | アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル | 気道感染症の処置のための方法及び医薬組成物 |
WO2015011254A1 (en) | 2013-07-26 | 2015-01-29 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Methods and pharmaceutical compositions for the treatment of bacterial infections |
KR102524577B1 (ko) | 2019-04-22 | 2023-04-21 | 전남대학교산학협력단 | 플라젤린 융합 단백질 및 이의 용도 |
FR3114970B1 (fr) | 2020-10-08 | 2023-06-30 | Univ Tours | Combinaison d’anticorps inhalés avec des agents immunomodulateurs pour le traitement ou la prévention d’inféctions respiratoires |
KR102461645B1 (ko) * | 2020-10-20 | 2022-11-01 | 전남대학교산학협력단 | 플라젤린 융합 단백질을 포함하는 비알코올성 지방간질환 또는 대사증후군 예방 또는 치료용 조성물 |
WO2023275292A2 (en) | 2021-06-30 | 2023-01-05 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Aerosol composition for pulmonary delivery of flagellin |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0241546A4 (en) * | 1985-10-11 | 1988-11-02 | Genetics Inst | METHOD FOR PRODUCING HETEROLOGICAL PROTEINS. |
-
1987
- 1987-03-11 EP EP87103488A patent/EP0237045B1/en not_active Expired - Lifetime
- 1987-03-11 DE DE87103488T patent/DE3787820T2/de not_active Expired - Fee Related
- 1987-03-11 US US07/024,992 patent/US4886748A/en not_active Expired - Lifetime
- 1987-03-11 ES ES87103488T patent/ES2060580T3/es not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DE3787820T2 (de) | 1994-04-28 |
DE3787820D1 (de) | 1993-11-25 |
EP0237045B1 (en) | 1993-10-20 |
EP0237045A2 (en) | 1987-09-16 |
EP0237045A3 (en) | 1988-11-02 |
US4886748A (en) | 1989-12-12 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ES2060580T3 (es) | Adn que tiene una secuencia adn codificante de la proteina flagelina y vector que la contiene. | |
MX6339E (es) | Metodo para elaborar dentro de un huesped bacteriano y excretar a traves de la membrana de la celula huesped,una proteina o un polipeptido seleccionados | |
ES2127829T3 (es) | Expresion de proteinas recombinantes fusionadas en bacterias atenuadas. | |
CY1113218T1 (el) | Υβριδιο και διμερης εκφραση πρωτεϊνων ναϊσσεριας | |
ES2077666T3 (es) | Proteina de union a fibronectina y su preparacion. | |
PT93910A (pt) | Prodesso para a preparacao de uma proteina heterologa mediante secrecao a partir de uma celula | |
ATE118352T1 (de) | Erreger der mysteriösen schweinekrankheit,impfstoff-zusammensetzungen und diagnose kits. | |
DE69523052D1 (de) | Gereinigte myceliophthora laccasen und nukleinsäuren dafür kodierend | |
ES2153037T3 (es) | Lacasas purificadas de scytalidium y acidos nucleicos que las codifican. | |
DE69926647D1 (de) | Durch molekulares pathogenicid vermittelte krankheitsresistenz in pflanzen | |
BR1100650A (pt) | Proteìnas de superfìcie cd44 variantes, sequências de dna que as codificam, anticorpos contra estas proteìnas, bem como a sua utilização no diagnóstico e na terapia | |
AR246765A1 (es) | Novedosos genes y secuencias de adn del factor i del crecimiento del tipo de la preproinsulina humana. | |
KR890000517A (ko) | 하이브리드 단백질 c 및 이의 제조방법 | |
FR2649982B1 (fr) | Membrane biologique artificielle | |
DE3878231T2 (de) | Neues cadiodilatin-fragment, prozess zu dessen herstellung und dessen anwendung. | |
NO930428D0 (no) | Proteinstrukturen til plantetoksingelonin | |
DE3881023D1 (de) | Proteine, expressionsvektoren, die fuer solche proteine kodierende dna enthalten und zellen, die solche expressionsvektoren enthalten. | |
ES2090357T3 (es) | Una proteina de union a colageno asi como su preparacion. | |
KR910003095A (ko) | 사람의 단백질 c변이체 및 이의 제조방법 | |
KR890006253A (ko) | 가금류내의 E.coli septicaemia에 대한 백신 | |
ES2074179T3 (es) | Haptenos marcados mejorados, procedimiento para su preparacion, asi como empleo de estos haptenos marcados en inmunoensayos. | |
DE3886443D1 (de) | Menschliche Rhinoviruspeptide. | |
ES2063223T5 (es) | Anticuerpos contra secuencias de aminoacidos muy conservadas de sustancias inmunogenas, procedimiento para la preparacion de estos anticuerpos, asi como su utilizacion en inmunoensayos. | |
DK0938555T3 (da) | Konstruktioner og komplekser af cyclin E | |
ES2104130T3 (es) | Adn hibrido, que codifica una subunidad de una toxina bacteriana con una hemolisina. |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
Ref document number: 237045 Country of ref document: ES |