ES2054618T3 - Vectores de expresion de dna recombinante y compuestos de dna que codifican la isopenicilina n sintetasa de penicillium chrysogenum. - Google Patents

Vectores de expresion de dna recombinante y compuestos de dna que codifican la isopenicilina n sintetasa de penicillium chrysogenum.

Info

Publication number
ES2054618T3
ES2054618T3 ES86309075T ES86309075T ES2054618T3 ES 2054618 T3 ES2054618 T3 ES 2054618T3 ES 86309075 T ES86309075 T ES 86309075T ES 86309075 T ES86309075 T ES 86309075T ES 2054618 T3 ES2054618 T3 ES 2054618T3
Authority
ES
Spain
Prior art keywords
isopenicillin
synthetase
dna
expression vectors
chrysogenum
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES86309075T
Other languages
English (en)
Inventor
Lucinda Gayle Carr
Thomas Dominick Ingolia
Stephen Wyatt Queener
Paul Luther Skatrud
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Application granted granted Critical
Publication of ES2054618T3 publication Critical patent/ES2054618T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Un procedimiento para la preparación de un vector de DNA recombinante, que comprende al menos una parte del operón isopenicilina N sintetasa de Penicillium chrysogenum, caracterizado por la incubación de un cultivo de E. coli/K12 JM109/pLC2, lisis de las células incubadas, separación del DNA de las células de los restos celulares mediante centrifugación, y extracción de los restos con fenol tamponado, y aislamiento del DNA plasmídico.
ES86309075T 1985-11-25 1986-11-20 Vectores de expresion de dna recombinante y compuestos de dna que codifican la isopenicilina n sintetasa de penicillium chrysogenum. Expired - Lifetime ES2054618T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/801,523 US4892819A (en) 1985-11-25 1985-11-25 Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from penicillium chrysogenum

Publications (1)

Publication Number Publication Date
ES2054618T3 true ES2054618T3 (es) 1994-08-16

Family

ID=25181331

Family Applications (1)

Application Number Title Priority Date Filing Date
ES86309075T Expired - Lifetime ES2054618T3 (es) 1985-11-25 1986-11-20 Vectores de expresion de dna recombinante y compuestos de dna que codifican la isopenicilina n sintetasa de penicillium chrysogenum.

Country Status (12)

Country Link
US (1) US4892819A (es)
EP (1) EP0225128B1 (es)
JP (1) JPH0773499B2 (es)
AT (1) ATE87974T1 (es)
AU (1) AU599343B2 (es)
CA (1) CA1309675C (es)
DE (1) DE3688236T2 (es)
DK (1) DK561886A (es)
ES (1) ES2054618T3 (es)
HU (1) HU206519B (es)
IE (1) IE59776B1 (es)
IL (1) IL80710A0 (es)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES500966A0 (es) 1980-04-03 1982-12-16 Biogen Nv Un metodo de producir un polipeptido que muestra una actividad immunologica obiologica de interferon de fibroblasto hu- mano.
DE3683080D1 (de) * 1985-04-22 1992-02-06 Lilly Co Eli Rekombinante dns-expressionsvektoren und dns-verbindungen.
US4892819A (en) * 1985-11-25 1990-01-09 Eli Lilly And Company Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from penicillium chrysogenum
GB8607502D0 (en) * 1986-03-26 1986-04-30 Antibioticos Sa Penicillium chrysogenum
US6709859B1 (en) * 1986-01-31 2004-03-23 Beecham Group P.L.C. Chromosomal DNA Fragments Encoding Enzymes for Encoding B-Lactam Biosynthesis Enzymes, and Vector and Transformants for Their Expression
DE3789880T2 (de) * 1986-01-31 1994-09-08 Beecham Group Plc Isolierung und Expression von bei der Biosynthese von Beta-Laktamen beteiligten Genen.
IL85535A (en) * 1987-03-04 1994-02-27 Lilly Co Eli AND Recombinant expression vectors encoding datastoxysplosporin C synthetase and datacetylsephalosporin C synthetase
GB2208077A (en) * 1987-04-24 1989-02-22 Antibioticos Sa Isopenicillin n synthetase gene derived from aspergillus nidulans
EP0303364A1 (en) * 1987-08-10 1989-02-15 The Governors of the University of Alberta Cloning and nucleotide sequence determination of the isopenicillin N synthetase gene from streptomyces clavuligerus
US4885252A (en) * 1987-09-08 1989-12-05 Eli Lilly And Company Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from aspergillus nidulans
EP0311273A1 (en) * 1987-09-24 1989-04-12 Beecham Group Plc Transformation system for Penicillium
US4950603A (en) * 1987-11-02 1990-08-21 Eli Lilly And Company Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from Streptomyces lipmanii
US6258555B1 (en) 1987-12-09 2001-07-10 Beecham Group P.L.C. DNA encoding ACV synthetase
GB8728811D0 (en) * 1987-12-09 1988-01-27 Beecham Group Plc Novel substance
FI104264B1 (fi) * 1988-08-11 1999-12-15 Dsm Nv Menetelmä sekundääristen metaboliittien tuoton parantamiseksi käyttämällä rykelmöityjä biosynteettisiä geenejä
US5108918A (en) * 1988-08-11 1992-04-28 Gist-Brocades Method for identifying and using biosynthetic genes for enhanced production of secondary metabolites
US5589385A (en) * 1990-07-26 1996-12-31 American Cyanamid Company Cloning of the biosynthetic pathway for chlortetracycline and tetracycline formation and cosmids useful therein
JPH06503960A (ja) * 1990-12-10 1994-05-12 ジェネンコア　インターナショナル　インコーポレーテッド ＴＲＩＣＨＯＤＥＲＭＡ　ＲＥＥＳＥＩのβ−グルコシダーゼ遺伝子のクローニングおよび増幅によるセルロースの改良糖化
US6103464A (en) * 1990-12-10 2000-08-15 Genencor International, Inc. Method of detecting DNA encoding a β-glucosidase from a filamentous fungus
US6524812B1 (en) 1993-10-07 2003-02-25 Regents Of The University Of Minnesota Genes encoding resistance to DNA alkylating agents
US6495348B1 (en) * 1993-10-07 2002-12-17 Regents Of The University Of Minnesota Mitomycin biosynthetic gene cluster
EP0783581B1 (en) * 1994-09-28 2006-07-26 Novozymes A/S Process for the production of secondary metabolites
USH2071H1 (en) * 1996-12-13 2003-07-01 Eli Lilly And Company Streptococcus pneumoniae gene HI0454
US6284483B1 (en) * 1999-10-06 2001-09-04 Board Of Trustees Operating Michigan State University Modified synthetases to produce penicillins and cephalosporins under the control of bicarbonate
US20030194784A1 (en) * 2001-04-17 2003-10-16 Sherman David H. DNA encoding methymycin and pikromycin
ES2400562T3 (es) * 2003-05-28 2013-04-10 Dsm Sinochem Pharmaceuticals Netherlands B.V. Compuesto de cefem

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4178210A (en) * 1977-03-07 1979-12-11 Massachusetts Institute Of Technology Accellular synthesis of cephalosporins
DE2759053A1 (de) * 1977-12-30 1979-07-12 Uhlin Verfahren zum herstellen von genprodukten von plasmid-dns
US4307192A (en) * 1979-05-17 1981-12-22 Massachusetts Institute Of Technology Cell-free synthesis of deacetoxycephalosporin C
US4618578A (en) * 1984-07-17 1986-10-21 Chiron Corporation Expression of glycoprotein D of herpes simplex virus
DE3683080D1 (de) * 1985-04-22 1992-02-06 Lilly Co Eli Rekombinante dns-expressionsvektoren und dns-verbindungen.
US4892819A (en) * 1985-11-25 1990-01-09 Eli Lilly And Company Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from penicillium chrysogenum

Also Published As

Publication number Publication date
US4892819A (en) 1990-01-09
ATE87974T1 (de) 1993-04-15
IE59776B1 (en) 1994-04-06
HU206519B (en) 1992-11-30
JPS62130691A (ja) 1987-06-12
HUT44801A (en) 1988-04-28
EP0225128B1 (en) 1993-04-07
EP0225128A1 (en) 1987-06-10
IE863085L (en) 1987-05-25
AU6561986A (en) 1987-05-28
JPH0773499B2 (ja) 1995-08-09
CA1309675C (en) 1992-11-03
DE3688236D1 (de) 1993-05-13
DK561886A (da) 1987-05-26
IL80710A0 (en) 1987-02-27
AU599343B2 (en) 1990-07-19
DK561886D0 (da) 1986-11-24
DE3688236T2 (de) 1993-08-12

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