DE3688236T2 - Rekombinante dna-expressionsvektoren und dna-zusammensetzungen, die isopenicillin-n-synthetase aus penicillium chrysogenum codieren. - Google Patents

Rekombinante dna-expressionsvektoren und dna-zusammensetzungen, die isopenicillin-n-synthetase aus penicillium chrysogenum codieren.

Info

Publication number
DE3688236T2
DE3688236T2 DE8686309075T DE3688236T DE3688236T2 DE 3688236 T2 DE3688236 T2 DE 3688236T2 DE 8686309075 T DE8686309075 T DE 8686309075T DE 3688236 T DE3688236 T DE 3688236T DE 3688236 T2 DE3688236 T2 DE 3688236T2
Authority
DE
Germany
Prior art keywords
synthetase
isopenicillin
dna
expression vectors
chrysogenum
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE8686309075T
Other languages
English (en)
Other versions
DE3688236D1 (de
Inventor
Lucinda Gayle Carr
Thomas Dominick Ingolia
Stephen Wyatt Queener
Paul Luther Skatrud
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Publication of DE3688236D1 publication Critical patent/DE3688236D1/de
Application granted granted Critical
Publication of DE3688236T2 publication Critical patent/DE3688236T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)
  • Peptides Or Proteins (AREA)
DE8686309075T 1985-11-25 1986-11-20 Rekombinante dna-expressionsvektoren und dna-zusammensetzungen, die isopenicillin-n-synthetase aus penicillium chrysogenum codieren. Expired - Fee Related DE3688236T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/801,523 US4892819A (en) 1985-11-25 1985-11-25 Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from penicillium chrysogenum

Publications (2)

Publication Number Publication Date
DE3688236D1 DE3688236D1 (de) 1993-05-13
DE3688236T2 true DE3688236T2 (de) 1993-08-12

Family

ID=25181331

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8686309075T Expired - Fee Related DE3688236T2 (de) 1985-11-25 1986-11-20 Rekombinante dna-expressionsvektoren und dna-zusammensetzungen, die isopenicillin-n-synthetase aus penicillium chrysogenum codieren.

Country Status (12)

Country Link
US (1) US4892819A (de)
EP (1) EP0225128B1 (de)
JP (1) JPH0773499B2 (de)
AT (1) ATE87974T1 (de)
AU (1) AU599343B2 (de)
CA (1) CA1309675C (de)
DE (1) DE3688236T2 (de)
DK (1) DK561886A (de)
ES (1) ES2054618T3 (de)
HU (1) HU206519B (de)
IE (1) IE59776B1 (de)
IL (1) IL80710A0 (de)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0041313B1 (de) 1980-04-03 1990-09-12 Biogen, Inc. DNS-Sequenzen, rekombinante DNS-Moleküle und Verfahren zur Herstellung von dem menschlichen Fibroblast-Interferon
EP0200425B1 (de) * 1985-04-22 1991-12-27 Eli Lilly And Company Rekombinante DNS-Expressionsvektoren und DNS-Verbindungen
US4892819A (en) * 1985-11-25 1990-01-09 Eli Lilly And Company Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from penicillium chrysogenum
GB8607502D0 (en) * 1986-03-26 1986-04-30 Antibioticos Sa Penicillium chrysogenum
US6709859B1 (en) 1986-01-31 2004-03-23 Beecham Group P.L.C. Chromosomal DNA Fragments Encoding Enzymes for Encoding B-Lactam Biosynthesis Enzymes, and Vector and Transformants for Their Expression
EP0233715B1 (de) * 1986-01-31 1994-05-25 BEECHAM GROUP plc Isolierung und Expression von bei der Biosynthese von Beta-Laktamen beteiligten Genen
IL85535A (en) * 1987-03-04 1994-02-27 Lilly Co Eli AND Recombinant expression vectors encoding datastoxysplosporin C synthetase and datacetylsephalosporin C synthetase
GB2208077A (en) * 1987-04-24 1989-02-22 Antibioticos Sa Isopenicillin n synthetase gene derived from aspergillus nidulans
EP0303364A1 (de) * 1987-08-10 1989-02-15 The Governors of the University of Alberta Klonierung und Bestimmung der Nukleotidesequenz vom Gen isopenicillin-N-synthetase aus Streptomyces clavuligerus
US4885252A (en) * 1987-09-08 1989-12-05 Eli Lilly And Company Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from aspergillus nidulans
EP0311273A1 (de) * 1987-09-24 1989-04-12 Beecham Group Plc Transformationssystem für Penicillium
US4950603A (en) * 1987-11-02 1990-08-21 Eli Lilly And Company Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from Streptomyces lipmanii
GB8728811D0 (en) * 1987-12-09 1988-01-27 Beecham Group Plc Novel substance
US6258555B1 (en) 1987-12-09 2001-07-10 Beecham Group P.L.C. DNA encoding ACV synthetase
US5108918A (en) * 1988-08-11 1992-04-28 Gist-Brocades Method for identifying and using biosynthetic genes for enhanced production of secondary metabolites
FI104264B (fi) * 1988-08-11 1999-12-15 Dsm Ip Assets Bv Menetelmä sekundääristen metaboliittien tuoton parantamiseksi käyttämällä rykelmöityjä biosynteettisiä geenejä
US5589385A (en) * 1990-07-26 1996-12-31 American Cyanamid Company Cloning of the biosynthetic pathway for chlortetracycline and tetracycline formation and cosmids useful therein
US6103464A (en) * 1990-12-10 2000-08-15 Genencor International, Inc. Method of detecting DNA encoding a β-glucosidase from a filamentous fungus
DE69133100T3 (de) * 1990-12-10 2015-09-03 Danisco Us Inc. Verbesserte saccharifizierung von zellulose durch klonierung und vervielfältigung des beta-glukosidase genes aus trichoderma reesei
US6524812B1 (en) 1993-10-07 2003-02-25 Regents Of The University Of Minnesota Genes encoding resistance to DNA alkylating agents
US6495348B1 (en) * 1993-10-07 2002-12-17 Regents Of The University Of Minnesota Mitomycin biosynthetic gene cluster
DE69535126D1 (de) * 1994-09-28 2006-09-07 Novozymes As Verfahren zur herstellung von sekundären metaboliten
US5958730A (en) * 1996-12-13 1999-09-28 Eli Lilly And Company Streptococcus pneumoniae gene sequence FtsY
US6284483B1 (en) * 1999-10-06 2001-09-04 Board Of Trustees Operating Michigan State University Modified synthetases to produce penicillins and cephalosporins under the control of bicarbonate
US20030194784A1 (en) * 2001-04-17 2003-10-16 Sherman David H. DNA encoding methymycin and pikromycin
WO2004106347A1 (en) * 2003-05-28 2004-12-09 Dsm Ip Assets B.V. Cephem compound

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4178210A (en) * 1977-03-07 1979-12-11 Massachusetts Institute Of Technology Accellular synthesis of cephalosporins
DE2759053A1 (de) * 1977-12-30 1979-07-12 Uhlin Verfahren zum herstellen von genprodukten von plasmid-dns
US4307192A (en) * 1979-05-17 1981-12-22 Massachusetts Institute Of Technology Cell-free synthesis of deacetoxycephalosporin C
US4618578A (en) * 1984-07-17 1986-10-21 Chiron Corporation Expression of glycoprotein D of herpes simplex virus
EP0200425B1 (de) * 1985-04-22 1991-12-27 Eli Lilly And Company Rekombinante DNS-Expressionsvektoren und DNS-Verbindungen
US4892819A (en) * 1985-11-25 1990-01-09 Eli Lilly And Company Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from penicillium chrysogenum

Also Published As

Publication number Publication date
IE59776B1 (en) 1994-04-06
HU206519B (en) 1992-11-30
DE3688236D1 (de) 1993-05-13
DK561886D0 (da) 1986-11-24
IL80710A0 (en) 1987-02-27
US4892819A (en) 1990-01-09
HUT44801A (en) 1988-04-28
EP0225128A1 (de) 1987-06-10
ATE87974T1 (de) 1993-04-15
JPH0773499B2 (ja) 1995-08-09
IE863085L (en) 1987-05-25
EP0225128B1 (de) 1993-04-07
JPS62130691A (ja) 1987-06-12
DK561886A (da) 1987-05-26
ES2054618T3 (es) 1994-08-16
AU6561986A (en) 1987-05-28
CA1309675C (en) 1992-11-03
AU599343B2 (en) 1990-07-19

Similar Documents

Publication Publication Date Title
DE3688236T2 (de) Rekombinante dna-expressionsvektoren und dna-zusammensetzungen, die isopenicillin-n-synthetase aus penicillium chrysogenum codieren.
AU6200886A (en) Heterologous polypeptides expressed in filamentous fungi, processes for making same, and vectors for making same
AU2474892A (en) Highly stable recombinant yeasts for the production of recombinant proteins
ES8900142A1 (es) Un procedimiento para aislar el plasmido it335
IE862307L (en) Production of foreign polypeptides in yeast hosts
AU551251B2 (en) Recombinant dna; chymosin production
DE3855633T2 (de) Rekombinante DNS-Expressionsvektoren und DNS-Zusammensetzungen, die für Deacetoxycephalosporin C-Synthetase und Deacetylcephalosporin C-Synthetase kodieren
HUT53149A (en) Process for producing dna encoding isopenicillin n-epimerase (racemase) and vector expressing recombinant dna
DE69120315D1 (de) Rekombinante DNA-Expressionsvektoren und DNA-Zusammensetzungen, die Isopenizillin N-Epimerase-Aktivität kodieren
IL87637A0 (en) Recombinant dna expression vectors and dna compounds that encode isopenicillin n synthetase from aspergillus nidulans
EP0146903A3 (de) Herstellung eines ein Hybrid-Interferon-Spezies kodierenden Vektors
EP0595334A3 (de) Mutanter AOX2 Promotor, diesen enthaltender Vektor, transformierte Zellen und Herstellung von heterologen Proteinen.
AU575331B2 (en) Improved e. coli plasmid containing modified 5' cipoprotein sequence

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee