ES2002653A6 - Procedimiento para la preparacion de 7-desaza-2'-desoxiguanosin-nucleotidos. - Google Patents

Procedimiento para la preparacion de 7-desaza-2'-desoxiguanosin-nucleotidos.

Info

Publication number
ES2002653A6
ES2002653A6 ES8601028A ES8601028A ES2002653A6 ES 2002653 A6 ES2002653 A6 ES 2002653A6 ES 8601028 A ES8601028 A ES 8601028A ES 8601028 A ES8601028 A ES 8601028A ES 2002653 A6 ES2002653 A6 ES 2002653A6
Authority
ES
Spain
Prior art keywords
nucleotides
deaza
preparation
deoxyguanosin
nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES8601028A
Other languages
English (en)
Spanish (es)
Inventor
Frank Seela
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=6278727&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2002653(A6) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of ES2002653A6 publication Critical patent/ES2002653A6/es
Expired legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16Purine radicals
    • C07H19/20Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/14Pyrrolo-pyrimidine radicals

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ES8601028A 1985-08-16 1986-08-12 Procedimiento para la preparacion de 7-desaza-2'-desoxiguanosin-nucleotidos. Expired ES2002653A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19853529478 DE3529478A1 (de) 1985-08-16 1985-08-16 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung

Publications (1)

Publication Number Publication Date
ES2002653A6 true ES2002653A6 (es) 1988-09-16

Family

ID=6278727

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8601028A Expired ES2002653A6 (es) 1985-08-16 1986-08-12 Procedimiento para la preparacion de 7-desaza-2'-desoxiguanosin-nucleotidos.

Country Status (6)

Country Link
US (3) US4804748A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
EP (1) EP0212536B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
JP (1) JPS6245596A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
AT (1) ATE57383T1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
DE (2) DE3529478A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
ES (1) ES2002653A6 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)

Families Citing this family (113)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6004446A (en) * 1984-03-29 1999-12-21 Li-Cor, Inc. DNA Sequencing
US6086737A (en) * 1984-03-29 2000-07-11 Li-Cor, Inc. Sequencing near infrared and infrared fluorescence labeled DNA for detecting using laser diodes and suitable labels therefor
US5863403A (en) * 1984-03-29 1999-01-26 The Board Of Regents Of The University Of Nebraska Digital DNA typing
US6207421B1 (en) 1984-03-29 2001-03-27 Li-Cor, Inc. DNA sequencing and DNA terminators
US5571388A (en) * 1984-03-29 1996-11-05 Li-Cor, Inc. Sequencing near infrared and infrared fluorescense labeled DNA for detecting using laser diodes and suitable labels thereof
US5360523A (en) * 1984-03-29 1994-11-01 Li-Cor, Inc. DNA sequencing
US4729947A (en) * 1984-03-29 1988-03-08 The Board Of Regents Of The University Of Nebraska DNA sequencing
DE3529478A1 (de) * 1985-08-16 1987-02-19 Boehringer Mannheim Gmbh 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung
US6211158B1 (en) * 1987-04-10 2001-04-03 Roche Diagnostics Gmbh Desazapurine-nucleotide derivatives, processes for the preparation thereof, pharmaceutical compositions containing them and the use thereof for nucleic acid sequencing and as antiviral agents
DE3739366A1 (de) * 1987-04-10 1988-10-27 Boehringer Mannheim Gmbh Desaza-purin-nucleosid-derivate, verfahren zu deren herstellung sowie deren verwendung bei der nucleinsaeure-sequenzierung sowie als antivirale mittel
US5459255A (en) * 1990-01-11 1995-10-17 Isis Pharmaceuticals, Inc. N-2 substituted purines
US6200747B1 (en) 1992-01-28 2001-03-13 North Shore University Hospital Research Corp. Method and kits for detection of fragile X specific, GC-rich DNA sequences
DE4438918A1 (de) * 1994-11-04 1996-05-09 Hoechst Ag Modifizierte Oligonukleotide, deren Herstellung sowie deren Verwendung
US6150510A (en) * 1995-11-06 2000-11-21 Aventis Pharma Deutschland Gmbh Modified oligonucleotides, their preparation and their use
AU6485996A (en) * 1995-07-06 1997-02-05 Board Of Regents, The University Of Texas System Methods and compositions for modulation and inhibition of teomerase
US6004939A (en) * 1995-07-06 1999-12-21 Ctrc Research Foundation Board Of Regents Methods for modulation and inhibition of telomerase
US6291164B1 (en) 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
GB2323357B (en) * 1997-03-20 1999-09-29 Amersham Pharm Biotech Inc Derivatives of 7-deaza-2-deoxy-guanosine-5'-triphosphate, preparation and use thereof
US6906185B1 (en) 1997-03-20 2005-06-14 Amersham Biosciences Corp. Derivatives of 7-deaza -2′-deoxyguanosine-5'-triphosphate, preparation and use thereof
US6660845B1 (en) 1999-11-23 2003-12-09 Epoch Biosciences, Inc. Non-aggregating, non-quenching oligomers comprising nucleotide analogues; methods of synthesis and use thereof
AU2001296645A1 (en) 2000-10-06 2002-04-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding dna and rna
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US20040063109A2 (en) 2002-01-25 2004-04-01 Applera Corporation Single-tube, ready-to-use assay kits, and methods using same
US20060009409A1 (en) * 2002-02-01 2006-01-12 Woolf Tod M Double-stranded oligonucleotides
EP2213737B1 (en) 2002-02-01 2012-11-07 Life Technologies Corporation Double-stranded oligonucleotides
EP1572902B1 (en) * 2002-02-01 2014-06-11 Life Technologies Corporation HIGH POTENCY siRNAS FOR REDUCING THE EXPRESSION OF TARGET GENES
US20040248094A1 (en) * 2002-06-12 2004-12-09 Ford Lance P. Methods and compositions relating to labeled RNA molecules that reduce gene expression
US20100075423A1 (en) * 2002-06-12 2010-03-25 Life Technologies Corporation Methods and compositions relating to polypeptides with rnase iii domains that mediate rna interference
US20040029275A1 (en) * 2002-08-10 2004-02-12 David Brown Methods and compositions for reducing target gene expression using cocktails of siRNAs or constructs expressing siRNAs
KR100890885B1 (ko) 2003-03-31 2009-03-31 에프. 호프만-라 로슈 아게 일본 뇌염 바이러스 혈청군의 구성원을 포함하는, 특정플라비바이러스 검출용 조성물 및 방법
US7572581B2 (en) * 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
US7947817B2 (en) * 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
WO2005044836A2 (de) 2003-11-05 2005-05-19 Genovoxx Gmbh Makromolekulare nukleotidverbindungen und methoden zu deren anwendung
US7158373B2 (en) * 2004-03-08 2007-01-02 Originatic Llc Electronic device having a keyboard rotatable about an axis
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
WO2005118806A2 (en) 2004-05-28 2005-12-15 Ambion, Inc. METHODS AND COMPOSITIONS INVOLVING MicroRNA
US7745125B2 (en) * 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
WO2006016978A1 (en) * 2004-06-30 2006-02-16 Applera Corporation Analog probe complexes
EP2281889B1 (en) 2004-11-12 2014-07-30 Asuragen, Inc. Methods and compositions involving miRNA and miRNA inhibitor molecules
US7842794B2 (en) 2004-12-17 2010-11-30 Roche Molecular Systems, Inc. Reagents and methods for detecting Neisseria gonorrhoeae
US20060292586A1 (en) * 2004-12-17 2006-12-28 Schroth Gary P ID-tag complexes, arrays, and methods of use thereof
US20060286590A1 (en) * 2005-06-20 2006-12-21 Applera Corporation. Cis-regulatory modules
US9169510B2 (en) 2005-06-21 2015-10-27 The Trustees Of Columbia University In The City Of New York Pyrosequencing methods and related compositions
US7465561B2 (en) 2005-06-30 2008-12-16 Roche Molecular Systems, Inc. Probes and methods for hepatitis C virus typing using single probe analysis
WO2007053719A2 (en) 2005-10-31 2007-05-10 The Trustees Of Columbia University In The City Of New York Chemically cleavable 3'-o-allyl-dntp-allyl-fluorophore fluorescent nucleotide analogues and related methods
WO2007053702A2 (en) 2005-10-31 2007-05-10 The Trustees Of Columbia University In The City Of New York Synthesis of four color 3'-o-allyl modified photocleavable fluorescent nucleotides and related methods
US9677123B2 (en) 2006-03-15 2017-06-13 Siemens Healthcare Diagnostics Inc. Degenerate nucleobase analogs
WO2008036765A2 (en) 2006-09-19 2008-03-27 Asuragen, Inc. Micrornas differentially expressed in pancreatic diseases and uses thereof
CA2663962A1 (en) 2006-09-19 2008-03-27 Asuragen, Inc. Mir-15, mir-26, mir-31,mir-145, mir-147, mir-188, mir-215, mir-216, mir-331, mmu-mir-292-3p regulated genes and pathways as targets for therapeutic intervention
WO2008069973A2 (en) 2006-12-01 2008-06-12 The Trustees Of Columbia University In The City Of New York Four-color dna sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US7858772B2 (en) * 2006-12-22 2010-12-28 Roche Molecular Systems, Inc. Compounds and methods for synthesis and purification of oligonucleotides
US8399194B2 (en) 2006-12-26 2013-03-19 Siemens Healthcare Diagnostics Inc. Heteropolynucleotide duplexes with purine—purine base pairing
EP3431615A3 (en) 2007-10-19 2019-02-20 The Trustees of Columbia University in the City of New York Dna sequencing with non-fluorescent nucleotide reversible terminators cleavable label modified nucleotide terminators
US10150990B2 (en) 2008-04-21 2018-12-11 Roche Molecular Systems, Inc. Ribonucleotide tag nucleic acid detection
US8258111B2 (en) 2008-05-08 2012-09-04 The Johns Hopkins University Compositions and methods related to miRNA modulation of neovascularization or angiogenesis
CA2740913C (en) * 2008-10-20 2015-02-10 F. Hoffmann-La Roche Ag Improved allele-specific amplification
US8206929B2 (en) 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
US8039215B2 (en) 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
US20120142004A1 (en) 2009-05-21 2012-06-07 Siemens Healthcare Diagnostics Inc. Universal Tags With Non-Natural Nucleobases
US8409802B2 (en) 2009-08-14 2013-04-02 Roche Molecular Systems, Inc. Format of probes to detect nucleic acid differences
WO2011050938A1 (de) 2009-10-26 2011-05-05 Genovoxx Gmbh Konjugate von nukleotiden und methoden zu deren anwendung
US9238832B2 (en) 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
US8614071B2 (en) 2009-12-11 2013-12-24 Roche Molecular Systems, Inc. Preferential amplification of mRNA over DNA using chemically modified primers
EP3822363B1 (en) 2010-02-05 2023-09-06 Quest Diagnostics Investments Incorporated Method to detect repeat sequence motifs in nucleic acid
ES2631458T3 (es) 2010-03-04 2017-08-31 Interna Technologies B.V. Molécula de ARNmi definida por su fuente y sus usos terapéuticos en el cáncer asociado a la EMT
NZ605420A (en) 2010-07-06 2015-02-27 Interna Technologies Bv Mirna and its diagnostic and therapeutic uses in diseases or conditions associated with melanoma, or in diseases or conditions associated with activated braf pathway
WO2012034007A2 (en) 2010-09-10 2012-03-15 Bio-Rad Laboratories, Inc. Size selection of dna for chromatin analysis
WO2012038049A2 (en) 2010-09-22 2012-03-29 Roche Diagnostics Gmbh Amplification of distant nucleic acid targets using engineered primers
CA2817882C (en) 2010-11-17 2022-08-23 Asuragen, Inc. Mirnas as biomarkers for distinguishing benign from malignant thyroid neoplasms
EP2474617A1 (en) 2011-01-11 2012-07-11 InteRNA Technologies BV Mir for treating neo-angiogenesis
EP3272885B1 (en) 2011-01-14 2020-04-22 Life Technologies Corporation Methods for identification, and quantification of mirnas
US20120208193A1 (en) 2011-02-15 2012-08-16 Bio-Rad Laboratories, Inc. Detecting methylation in a subpopulation of genomic dna
WO2012146377A1 (de) 2011-04-27 2012-11-01 Dmitry Cherkasov Methoden und komponenten zur detektion von nukleinsäureketten
US20150086981A1 (en) 2011-05-04 2015-03-26 Genovoxx Gmbh Nucleoside-triphosphate conjugate and methods for the use thereof
US9624539B2 (en) 2011-05-23 2017-04-18 The Trustees Of Columbia University In The City Of New York DNA sequencing by synthesis using Raman and infrared spectroscopy detection
US8728987B2 (en) 2011-08-03 2014-05-20 Bio-Rad Laboratories, Inc. Filtering small nucleic acids using permeabilized cells
WO2013040251A2 (en) 2011-09-13 2013-03-21 Asurgen, Inc. Methods and compositions involving mir-135b for distinguishing pancreatic cancer from benign pancreatic disease
CA2847943C (en) 2011-09-23 2017-08-29 F.Hoffmann-La Roche Ag Use of g-clamp for improved allele-specific pcr
WO2013063519A1 (en) 2011-10-26 2013-05-02 Asuragen, Inc. Methods and compositions involving mirna expression levels for distinguishing pancreatic cysts
US9115394B2 (en) 2011-12-22 2015-08-25 Roche Molecular Systems, Inc. Methods and reagents for reducing non-specific amplification
US10174314B2 (en) 2011-12-22 2019-01-08 Interna Technologies B.V. MiRNA for treating head and neck cancer
WO2014015098A1 (en) 2012-07-18 2014-01-23 Siemens Healthcare Diagnostics Inc. A method of normalizing biological samples
US20140100124A1 (en) 2012-10-04 2014-04-10 Asuragen, Inc. Diagnostic mirnas for differential diagnosis of incidental pancreatic cystic lesions
EP3800256A1 (en) 2012-11-06 2021-04-07 InteRNA Technologies B.V. Combination to be used in therapeutic use against diseases or conditions associated with melanoma, or in diseases or conditions associated with activated b-raf pathway
DK2971098T3 (en) 2013-03-14 2019-02-18 Translate Bio Inc QUANTITATIVE ASSESSMENT FOR CAPE EFFECTIVENESS OF MESSENGER RNA
BR112015022505A2 (pt) 2013-03-14 2017-10-24 Shire Human Genetic Therapies avaliação quantitativa para eficiência do cap de rna mensageiro
US9828629B2 (en) 2013-03-15 2017-11-28 Roche Molecular Systems, Inc. Nucleic acid target identification by structure based probe cleavage
ES2935257T3 (es) 2013-03-15 2023-03-03 Univ Chicago Métodos y composiciones relacionadas con la actividad de las células T
US10648026B2 (en) 2013-03-15 2020-05-12 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
CN105722977A (zh) 2013-10-30 2016-06-29 绿色生活生物技术有限责任公司 用于柑橘绿化枯萎病的发病定量系统和治疗方法
US9637791B2 (en) 2013-12-20 2017-05-02 Roche Molecular Systems, Inc. Multiplexed nucleic acid target identification by strucutre based probe cleavage
US9243289B2 (en) 2013-12-23 2016-01-26 Roche Molecular Systems, Inc. Method for screening reagents used in PCR assays
WO2015123551A1 (en) 2014-02-13 2015-08-20 Du Luqin Microrna composition for the treatment of neuroblastoma
US9909169B2 (en) 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
CN104845967B (zh) 2015-04-15 2020-12-11 苏州新海生物科技股份有限公司 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用
WO2017201315A1 (en) 2016-05-18 2017-11-23 Roche Sequencing Solutions, Inc. Quantitative real time pcr amplification using an electrowetting-based device
ES2910080T3 (es) 2016-07-18 2022-05-11 Hoffmann La Roche Moldes asimétricos y procedimiento asimétrico de secuenciación de ácido nucleico
CN111566212A (zh) 2017-11-03 2020-08-21 因特尔纳技术有限公司 miRNA分子,等同物,安塔够妙或其来源用于治疗和/或诊断与神经元缺陷相关的病症和/或疾病或用于神经元生成和/或再生
EP3710820B1 (en) 2017-11-13 2025-06-11 F. Hoffmann-La Roche AG Devices for sample analysis using epitachophoresis
EP3752164A1 (en) 2018-02-12 2020-12-23 InteRNA Technologies B.V. Anticancer microrna and lipid formulations thereof
WO2020074742A1 (en) 2018-10-12 2020-04-16 F. Hoffmann-La Roche Ag Detection methods for epitachophoresis workflow automation
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
EP3969583A1 (en) 2019-05-14 2022-03-23 F. Hoffmann-La Roche AG Devices and methods for sample analysis
CA3145912A1 (en) 2019-08-01 2021-02-04 Adrian Horgan Increasing long-sequence yields in template-free enzymatic synthesis of polynucleotides.
WO2021259881A1 (en) 2020-06-22 2021-12-30 Illumina Cambridge Limited Nucleosides and nucleotides with 3' acetal blocking group
BR112023001129A2 (pt) 2020-07-24 2023-02-14 Univ California Pró-fármacos antivirais, formulações farmacêuticas e métodos
KR20240017865A (ko) 2021-06-04 2024-02-08 트랜슬레이트 바이오 인코포레이티드 Mrna 캡핑 효율의 정량적 평가를 위한 분석
WO2023102459A1 (en) 2021-12-03 2023-06-08 Medicinal Genomics Corporation Psilocybe assay
US20250059600A1 (en) 2021-12-09 2025-02-20 Roche Molecular Systems, Inc. Mass based detection of pcr amplicons
WO2024059493A1 (en) 2022-09-13 2024-03-21 Medicinal Genomics Corporation Psilocybe assay
WO2024104914A1 (en) 2022-11-14 2024-05-23 BioNTech SE Rna capping efficiency assay
IT202200026595A1 (it) 2022-12-22 2024-06-22 Fond Telethon Ets Nuovi inibitori di regolatori epigenetici
WO2024256674A1 (en) 2023-06-14 2024-12-19 Sanofi Methods of simultaneously identifying or quantifying capping and tailing modifications of messenger rna

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3321462A (en) * 1963-07-15 1967-05-23 Syntex Corp Process for the preparation of nucleoside polyphosphates
US3337530A (en) * 1965-09-20 1967-08-22 Upjohn Co Dinucleoside 3', 5' -and 2', 5'-phosphates containing one 7-deazapurin riboside moiety
US4500707A (en) * 1980-02-29 1985-02-19 University Patents, Inc. Nucleosides useful in the preparation of polynucleotides
DD225696A1 (de) * 1984-06-15 1985-08-07 Adw Ddr Verfahren zur herstellung von nucleosid-5'-di- und triphosphaten hoher spezifischer aktivitaet
DE3529478A1 (de) * 1985-08-16 1987-02-19 Boehringer Mannheim Gmbh 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung
US5142033A (en) * 1988-09-23 1992-08-25 Hoffmann-La Roche Inc. Structure-independent DNA amplification by the polymerase chain reaction
US5091310A (en) * 1988-09-23 1992-02-25 Cetus Corporation Structure-independent dna amplification by the polymerase chain reaction

Also Published As

Publication number Publication date
US5480980A (en) 1996-01-02
EP0212536A3 (en) 1988-03-16
DE3529478A1 (de) 1987-02-19
US5990303A (en) 1999-11-23
JPS638120B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 1988-02-19
DE3674861D1 (de) 1990-11-15
JPS6245596A (ja) 1987-02-27
ATE57383T1 (de) 1990-10-15
EP0212536A2 (de) 1987-03-04
EP0212536B1 (de) 1990-10-10
US4804748A (en) 1989-02-14

Similar Documents

Publication Publication Date Title
EP0212536A3 (en) 7-deaza-2'-deoxyguanosin-nucleotides, process for their preparation and their use in nucleic-acid sequencing
ES8703934A1 (es) Un metodo para detectar una secuencia de polinucleotidos
IL72499A0 (en) Detection of bacteria by nucleic acid hybridization
AU561382B2 (en) Identification of nucleic acid by hybridization probes
GR3021704T3 (en) Nucleic acid sequence amplification methods
ES8406477A1 (es) Procedimiento para la preparacion de b-carbolinas
IL81695A (en) Quantitative determination of the degree of amplification of genes and/or corresponding messenger rna and reagent kit for performing the determination
GB8810181D0 (en) Amperometric method for quantitative determination of 1 4-dihydronicotinamide adenine in solution
EP0329198A3 (en) Ribose- and 2-deoxyribose compounds
ATE10573T1 (de) Salzgemisch, verfahren zu seiner herstellung und verwendung.
GB2050602B (en) Method of and reagents for quantitative determination of cyclic nucleotides
ATE92498T1 (de) Primycinkomponenten und verfahren zur trennung des antibiotischen komplexes.
EP0167936A3 (en) Use of actinonin as an immunopotentiator
WO1994012518A3 (de) Reagenz zur kupplung veschiedener substanzen an nukleinsäuren sowie verfahren zu dessen herstellung
FR2594107B1 (fr) Procede de preparation de chlorate de metal alcalin
CA2210900A1 (en) Method and composition for stabilization of labelled nucleoside triphosphates
EP0205967A3 (en) H2o2-forming sarcosineoxidase, their preparation and use
ZA878464B (en) Method for the detection of nucleic acid hybrids
ES2000327A6 (es) Procedimiento para preparar flourotitanatos de metales alcalinos
EP0228264A3 (en) Tylosin derivatives, processes for their preparation, and novel processes involving tylosin derivatives
AU2687488A (en) Novel polynucleotide analogs, methods for inhibiting nucleic acid polymerases and methods for inducing synthesis of interferon
Frank-Kamenetskaya et al. Enzymatic synthesis of labeled nucleotides of high molar radioactivity.
EP0151245A3 (en) Soporific comprising lorazepam
YU43881B (en) Process for obtaining crystalline salt of s-6-fluor-4-(benzyloxi-carbonylamino )chroman-4-carbonic acid
ES8302088A1 (es) Procedimiento para la produccion de uricasa

Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20060814