EP4267721A1 - Souches bactériennes pour la production de biocellulose - Google Patents
Souches bactériennes pour la production de biocelluloseInfo
- Publication number
- EP4267721A1 EP4267721A1 EP21840612.2A EP21840612A EP4267721A1 EP 4267721 A1 EP4267721 A1 EP 4267721A1 EP 21840612 A EP21840612 A EP 21840612A EP 4267721 A1 EP4267721 A1 EP 4267721A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- biocellulose
- rscic
- dps
- efl
- access
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229920001340 Microbial cellulose Polymers 0.000 title claims abstract description 54
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 49
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 36
- 241000131407 Brevundimonas Species 0.000 claims abstract description 31
- 238000000034 method Methods 0.000 claims abstract description 22
- 238000011534 incubation Methods 0.000 claims description 25
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 17
- 239000008103 glucose Substances 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 239000001963 growth medium Substances 0.000 claims description 16
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 14
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 13
- 229930091371 Fructose Natural products 0.000 claims description 12
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 12
- 239000005715 Fructose Substances 0.000 claims description 12
- 238000010790 dilution Methods 0.000 claims description 10
- 239000012895 dilution Substances 0.000 claims description 10
- 239000013535 sea water Substances 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 5
- FOIXSVOLVBLSDH-UHFFFAOYSA-N Silver ion Chemical compound [Ag+] FOIXSVOLVBLSDH-UHFFFAOYSA-N 0.000 claims description 3
- 230000000845 anti-microbial effect Effects 0.000 claims description 3
- 238000010521 absorption reaction Methods 0.000 claims description 2
- 239000003242 anti bacterial agent Substances 0.000 claims description 2
- 229940088710 antibiotic agent Drugs 0.000 claims description 2
- 229920002678 cellulose Polymers 0.000 description 12
- 239000001913 cellulose Substances 0.000 description 12
- 241000894006 Bacteria Species 0.000 description 10
- 229940041514 candida albicans extract Drugs 0.000 description 10
- 238000003756 stirring Methods 0.000 description 10
- 239000012138 yeast extract Substances 0.000 description 10
- 229920002749 Bacterial cellulose Polymers 0.000 description 8
- 239000005016 bacterial cellulose Substances 0.000 description 8
- 244000235858 Acetobacter xylinum Species 0.000 description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 7
- 235000015097 nutrients Nutrition 0.000 description 7
- 235000002837 Acetobacter xylinum Nutrition 0.000 description 6
- 108020004465 16S ribosomal RNA Proteins 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 5
- 238000012512 characterization method Methods 0.000 description 5
- 230000003068 static effect Effects 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 241000194108 Bacillus licheniformis Species 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000013270 controlled release Methods 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- 238000001228 spectrum Methods 0.000 description 3
- 239000011782 vitamin Substances 0.000 description 3
- 235000013343 vitamin Nutrition 0.000 description 3
- 229940088594 vitamin Drugs 0.000 description 3
- 229930003231 vitamin Natural products 0.000 description 3
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 description 2
- 241000061154 Brevundimonas sp. Species 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- 229930195725 Mannitol Natural products 0.000 description 2
- 229920001046 Nanocellulose Polymers 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 230000008166 cellulose biosynthesis Effects 0.000 description 2
- 239000000594 mannitol Substances 0.000 description 2
- 235000010355 mannitol Nutrition 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 229960000907 methylthioninium chloride Drugs 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000002086 nanomaterial Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 230000029663 wound healing Effects 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241001150381 Bacillus altitudinis Species 0.000 description 1
- 108010023063 Bacto-peptone Proteins 0.000 description 1
- 241000131418 Brevundimonas vesicularis Species 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 241000222122 Candida albicans Species 0.000 description 1
- PTHCMJGKKRQCBF-UHFFFAOYSA-N Cellulose, microcrystalline Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC)C(CO)O1 PTHCMJGKKRQCBF-UHFFFAOYSA-N 0.000 description 1
- 241000283073 Equus caballus Species 0.000 description 1
- 238000005033 Fourier transform infrared spectroscopy Methods 0.000 description 1
- 238000004566 IR spectroscopy Methods 0.000 description 1
- 241000404158 Lonas Species 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000004378 air conditioning Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 229940095731 candida albicans Drugs 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000003937 drug carrier Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000009422 growth inhibiting effect Effects 0.000 description 1
- 125000001475 halogen functional group Chemical group 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000006193 liquid solution Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000006104 solid solution Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 239000003981 vehicle Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
- C12N9/1059—Cellulose synthases (2.4.1.12; 2.4.1.29)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
Definitions
- the present application relates to the field of microbiology and in particular isolated and verified bacterial strains for biocellulose production .
- a bacterial cultivation system for the production of cellulose gel (HSBC) containing Gluconacetobacter xylinus (G. xylinus) in an interface system between an aqueous and an oily phase in the presence of glucose and mannitol at 30°C is known (Horu Hoshia, Kazuyoshi Yamazakia, Yuki Satob, Takaya Shidaa, Takao Aoyagia, Production of hollow-type spherical bacterial cellulose as a controlled release device by newly designed floating cultivation, Heliyon 4, 2018, e00873) .
- the inventors of the present invention have isolated new bacterial strains belonging to the genus Bacillus and Brevundimonas capable of producing biocellulose.
- Said strains have peculiar characteristics compared to strains known in the art. In particular, they are able to synthesise biocellulose under room temperature conditions, i.e. without energy expenditure, compared to known processes which take place at a temperature of around 30°C. In the process, no specific saline solutions are used or prepared, but plain water is used, and the culture medium is not a limiting factor and different types can be used in the bacterial culture without affecting the success of the process for biocellulose production. In contrast to what is known in the art in which the culture media are added with glucose and mannitol, the former at concentrations of at least 3%, in the culture of the present invention glucose or fructose at 1.5% is added.
- Another object of the present ibnvention is the use of Brevundimonas efl Access no. DPS RE RSCIC 24 for biocellulose production.
- Still another object of the present invention is a process for biocellulose production including the following steps: a) incubation of Brevundimonas efl Access no. DPS RE RSCIC 24 at room temperature in the presence of culture medium suitable for bacterial growth until obtaining adequate bacterial growth;
- RECTIFIED SHEET (RULE 91) ISA/EP b ) dilution of the culture obtained at the end of step a ) with water and addition of glucose or fructose in an amount ranging between 1 . 5 and 2% of the total and subsequent incubation at room temperature until production of biocellulose by the bacterial culture .
- a further obj ect of the present invention is biocellulose obtained by means of the above process .
- Figure 1 shows a representative infrared spectroscopy (FTIR) spectrum of the materials obtained in static or stirring mode with both bacteria : the spectrum indicates that the material in question is cellulose .
- FTIR infrared spectroscopy
- Figure 2 shows a reference spectrum from Yim, S . M . ; Song, J . E . , Kim, H . R . Production and characterization of bacterial cellulose fabrics by nitrogen sources of tea and carbon sources of sugar, Process Biochemistry 2017 , 59 , 26-36
- biocellulose means cellulose of natural , microbial origin characterised by fibres having variable diameter .
- culture medium suitable for bacterial growth means solid or liquid solutions containing nutrients on which bacterial colonies are possible .
- the obj ect of the present invention is Brevundimonas efl Access n .
- DPS RE RSCIC 24 The obj ect of the present invention is Brevundimonas efl Access n .
- a further obj ect of the present invention is the use of Brevundimonas efl Access n .
- Another another obj ect of the present invention is a process for biocellulose production including the following steps : a ) incubation of Brevundimonas efl Access n . DPS RE RSCIC 24 at room temperature in the presence of culture medium suitable for bacterial growth until obtaining adequate bacterial growth; b ) dilution of the culture obtained at the end of step a ) with water, addition of glucose or fructose in an amount ranging between 1 . 5 and 2% of the total and subsequent incubation at room temperature until production of biocellulose by the bacterial culture .
- step a Brevundimonas ef l Access no .
- DPS RE RSCIC 24 is in combination with Bacillus ef l Access no .
- DPS RE RSCIC 23 is optionally in step a .
- a further obj ect of the present invention is biocellulose of bacterial origin obtained by a process comprising the following steps : a ) incubation of Brevundimonas ef l Access n . DPS RE RSCIC 24 at room temperature in the presence of culture medium suitable for bacterial growth until obtaining adequate bacterial growth; b ) dilution of the culture obtained at the end of step a ) with water and addition of glucose or fructose in an amount ranging between 1 . 5 and 2% of the total and subsequent incubation at room temperature until production of biocellulose by the bacterial culture .
- the obj ect of the present invention is also the use of biocellulose of bacterial origin obtained by a process comprising the following steps : a ) incubation of Brevundimonas efl Access n . DPS RE RSCIC 24 at room temperature in the presence of culture medium suitable for bacterial growth until obtaining adequate bacterial growth; b ) dilution of the culture obtained at the end of step a ) with water and addition of glucose or fructose in an amount ranging between 1 . 5 and 2% of the total and subsequent incubation at room temperature until production of biocellulose by the bacterial culture for the absorption of antibiotics and the subsequent slow release thereof into the environment .
- a further object of the present invention is the use of biocellulose of bacterial origin obtained by a process comprising the following steps : a) incubation of Brevundimonas efl Access n. DPS RE RSCIC 24 at room temperature in the presence of culture medium suitable for bacterial growth until obtaining adequate bacterial growth; b) dilution of the culture obtained at the end of step a) with water and addition of glucose or fructose in an amount ranging between 1.5 and 2% of the total and subsequent incubation at room temperature until production of biocellulose by the bacterial culture for the production of biocellulose filters.
- step a) Brevundimonas efl Access no.
- DPS RE RSCIC 24 is in combination with Bacillus efl Access no.
- DPS RE RSCIC 23 is optionally in step a) Brevundimonas efl Access no.
- the filters can be used in air conditioning or water filtration systems, for example in swimming pools and/or aquariums to block bacterial growth and maintain a healthy environment .
- the biocellulose spheres contain silver nanoparticles with antimicrobial activity.
- the bacterial culture medium can be selected from the group consisting of: Hestrin-Schramm, Yamanaka, Zhou, nutrient broth or yeast extract .
- yeast extract is yeast extract (0.5-1%) .
- the water is seawater or deionised water.
- the nutrient broth has the following composition:
- step a) and step b) the bacteria are incubated at a temperature ranging from 22 and 25 °C.
- step a) the bacteria are incubated statically or under stirring .
- the bacteria When in step a) the bacteria are incubated under stirring, they are preferably under stirring by means of a stirrer at a speed of about 150-200 rpm.
- the adequate bacterial growth is the achievement of an optical density of about 0.6 OD at 550 nanometres.
- step b) if the water is seawater in an amount equal to
- step b) if the water is deionised water it is in an amount equal to 90 % of the final volume.
- step b) glucose or fructose is present in an amount equal to 1.5%.
- the temperature is 22 °C.
- the obtained biocellulose is in the form of a sheet when in step a) the incubation is in static mode.
- the obtained biocellulose is in the form of spheres when in step a) the incubation is under stirring and preferably they have an average diameter ranging between 2 and 10 mm.
- the first strain Bacillus efl Access no. DPS RE RSCIC 23 was identified as belonging to the genus Bacillus on the basis of the 16S rDNA sequence, it is similar to Bacillus altitudinis, it is a gram-positive rod-shaped bacterium. SEQ ID . NO . 1 PRQKKA_03858 Bacillus 16S ribosomal RNA
- DPS RE RSCIC 24 was identified as belonging to the genus Brevundimonas on the basis of the 16S rDNA sequence
- DPS RE RSCIC 24 is similar to Brevundimonas vesicularis adapted to live in Martian conditions .
- sequences similar to the 16S rRNA gene there is one from the strain Brevundimonas sp . R-37024 isolated from a Lake Lundstrdm in Antarctica .
- Brevundimonas ef l possesses an enzyme involved in cellulose biosynthesis .
- the nutrient broth had the following composition:
- Yeast extract is a source of vitamins, particularly B group vitamins. Sodium chloride maintains the osmotic balance of the medium.
- the bacteria incubated at 22°C at 150-200 rpm of rotation.
- the culture is diluted with seawater H 2 0 (50 % of the final volume) or deionised water (90 % of the final volume) . and added with 1.5-2% glucose, and further incubated statically (without stirring) or with stirring at 22°C.
- biocellulose sheet is produced (static mode)
- biocellulose spheres are produced (stirring mode) by Bacillus efl Access no. DPS RE RSCIC 23 in nutrient broth diluted with 50% seawater and added with 1.5-2%.
- biocellulose sheet is produced (static mode)
- biocellulose spheres are produced (stirring mode) by Brevundimonas efl Access no.
- DPS RE RSCIC 24 of 25/11/2020 both cultivated in nutrient broth and in yeast extract occurs .
- biocellulose spheres The ability of the biocellulose spheres to absorb external substances as previously produced was further tested .
- Methylene blue is partially released into the culture medium: after 18 hours , about l/20th of the initial amount absorbed is released .
- Table 2 reports the production methods ( rotation speed, medium volume , flask size ) , yield and average size of the biocellulose spheres synthesised by Bacillus ef l and Brevundimonas efl after 5 days of culture .
- the biocellulose in the form of spheres was also soaked in a solution containing silver nanoparticles ( 20 mg/ml ) having antimicrobial activity, as described in the application incorporated herein for Italian patent reference no . 102019000014121 by the same inventors , 24 hours and subj ected to UV ray sterilisation for 10 minutes . Then the biocellulose spheres were placed in a plate with Muller hinton agar where a strain of Candida albicans was inoculated . The growth inhibitory effect of Candida was visible with a halo of about 4-5 mm even after 72 hours at 22 ° C .
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IT102020000031769A IT202000031769A1 (it) | 2020-12-22 | 2020-12-22 | Ceppi batterici per la produzione di biocellulosa |
PCT/EP2021/086800 WO2022136277A1 (fr) | 2020-12-22 | 2021-12-20 | Souches bactériennes pour la production de biocellulose |
Publications (1)
Publication Number | Publication Date |
---|---|
EP4267721A1 true EP4267721A1 (fr) | 2023-11-01 |
Family
ID=74875088
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP21840612.2A Pending EP4267721A1 (fr) | 2020-12-22 | 2021-12-20 | Souches bactériennes pour la production de biocellulose |
EP21824606.4A Pending EP4267720A1 (fr) | 2020-12-22 | 2021-12-20 | Souches bactériennes pour la production de biocellulose |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP21824606.4A Pending EP4267720A1 (fr) | 2020-12-22 | 2021-12-20 | Souches bactériennes pour la production de biocellulose |
Country Status (4)
Country | Link |
---|---|
EP (2) | EP4267721A1 (fr) |
KR (2) | KR20230124918A (fr) |
IT (1) | IT202000031769A1 (fr) |
WO (2) | WO2022136246A1 (fr) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IT202100017333A1 (it) * | 2021-07-01 | 2023-01-01 | Irides S R L | Produzione di biomateriali da prodotti di scarto alimentari da parte di ceppi batterici antartici |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR691410A (fr) | 1930-03-03 | 1930-10-21 | Jeu de direction indéréglable pour cycles, motocycles, etc. | |
IT201900014121A1 (it) | 2019-08-06 | 2021-02-06 | Univ Degli Studi Di Camerino | Marinomonas ef1 e rhodococcus ef1 per la produzione di metaboliti secondari |
-
2020
- 2020-12-22 IT IT102020000031769A patent/IT202000031769A1/it unknown
-
2021
- 2021-12-20 EP EP21840612.2A patent/EP4267721A1/fr active Pending
- 2021-12-20 KR KR1020237021057A patent/KR20230124918A/ko unknown
- 2021-12-20 WO PCT/EP2021/086747 patent/WO2022136246A1/fr unknown
- 2021-12-20 WO PCT/EP2021/086800 patent/WO2022136277A1/fr unknown
- 2021-12-20 EP EP21824606.4A patent/EP4267720A1/fr active Pending
- 2021-12-20 KR KR1020237021058A patent/KR20230124919A/ko unknown
Also Published As
Publication number | Publication date |
---|---|
WO2022136246A1 (fr) | 2022-06-30 |
EP4267720A1 (fr) | 2023-11-01 |
KR20230124919A (ko) | 2023-08-28 |
IT202000031769A1 (it) | 2022-06-22 |
WO2022136277A1 (fr) | 2022-06-30 |
KR20230124918A (ko) | 2023-08-28 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Niknezhad et al. | Production of xanthan gum by free and immobilized cells of Xanthomonas campestris and Xanthomonas pelargonii | |
JP5765859B2 (ja) | 黄色色素生成欠陥スフィンゴリピドアエロモナス及びジェランゴム生産における使用 | |
JP3720361B2 (ja) | ヒアルロン酸の生産方法及び手段 | |
MX2013015363A (es) | Procesos para elaborar derivados de quitina. | |
CN110257410B (zh) | 一种编码褐藻胶裂解酶的基因 | |
CN108410783B (zh) | 一种培养大肠杆菌发酵生产氨基葡萄糖的方法 | |
CN110438111A (zh) | 一种褐藻胶裂解酶及其应用 | |
EP4267721A1 (fr) | Souches bactériennes pour la production de biocellulose | |
JP5969390B2 (ja) | フコース含有細菌バイオポリマー | |
CN105670975B (zh) | 一种高产胞外多糖的假单胞菌株及其应用 | |
CN112662572B (zh) | 一种高产壳聚糖酶的菌株及其应用 | |
JP2001106608A (ja) | 植物成長促進剤 | |
Minimol et al. | Isolation and characterization of exopolysaccharide producing Bacillus cereus from Brown seaweed-Sargassum wightii | |
Rajendran et al. | Biocompatible nanofiber from exopolysaccharide produced by moderately halophilic Paenibacillus alvei | |
CN113413327B (zh) | 一种多糖类皮肤微生态化妆品的制备方法 | |
CN114437985B (zh) | 一株产气肠杆菌及其在合成微生物多糖中的应用 | |
JPH0549491A (ja) | 微細藻類から多糖類を生産する方法 | |
CN113151060B (zh) | 一种延长赖氨酸芽孢杆菌zcgt05及其应用 | |
CN111849814B (zh) | 一种降解透明质酸的不动杆菌及其培养方法和应用 | |
RU2740710C1 (ru) | Штамм бактерии Paenibacillus polymyxa - продуцент левана | |
AU625278B2 (en) | Polysaccharides, process for preparing them by culturing pseudomonas paucimobilis and application of the strains in agriculture | |
KR20080006931A (ko) | 해양 미생물 비브리오 에스피. 에스21 균주를 이용한알긴산 분해효소의 제조방법 | |
JP3079183B2 (ja) | 褐藻分解物の製造法 | |
CN116286512A (zh) | 一株产壳聚糖酶的芽孢杆菌及其应用 | |
JP3796540B2 (ja) | 黄色色素の製造方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: UNKNOWN |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE |
|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE |
|
17P | Request for examination filed |
Effective date: 20230713 |
|
AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR |
|
P01 | Opt-out of the competence of the unified patent court (upc) registered |
Effective date: 20240207 |
|
DAX | Request for extension of the european patent (deleted) | ||
RAV | Requested validation state of the european patent: fee paid |
Extension state: KH Effective date: 20230713 |