EP3965728A1 - Odermennigextrakt als umweltfreundliches mittel - Google Patents

Odermennigextrakt als umweltfreundliches mittel

Info

Publication number
EP3965728A1
EP3965728A1 EP20722610.1A EP20722610A EP3965728A1 EP 3965728 A1 EP3965728 A1 EP 3965728A1 EP 20722610 A EP20722610 A EP 20722610A EP 3965728 A1 EP3965728 A1 EP 3965728A1
Authority
EP
European Patent Office
Prior art keywords
extract
skin
agrimony
aerial parts
cosmetic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP20722610.1A
Other languages
English (en)
French (fr)
Inventor
Christel FIORINI
Philippe Joulia
Patrick Bogdanowicz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pierre Fabre Dermo Cosmetique SA
Original Assignee
Pierre Fabre Dermo Cosmetique SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pierre Fabre Dermo Cosmetique SA filed Critical Pierre Fabre Dermo Cosmetique SA
Publication of EP3965728A1 publication Critical patent/EP3965728A1/de
Pending legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/007—Preparations for dry skin

Definitions

  • TITLE Agrimony extract as an anti-pollution agent
  • the present invention relates to an extract of agrimony and to compositions containing this extract for an application in the field of cosmetics and dermatology for combating the harmful effects of pollution on the skin.
  • the official definition of pollution is "a degradation of the environment by the introduction into the air, water or soil of materials not naturally present in the environment".
  • air pollution continues to increase, mainly due to human activity, measures have been put in place to give priority to research aimed at reducing or controlling emissions of polluting gases.
  • the consequences of this atmospheric pollution not only have effects on a planetary scale such as the greenhouse effect and the destruction of the ozone layer, but also on a local level on health, well-being, skin and the dander of living beings.
  • the main pollutants have natural and anthropogenic origins.
  • particulates also called suspended particles and tobacco smoke.
  • the effect of these polluting factors on the skin is amplified when combined with other pollutants and / or solar radiation.
  • UV rays associated with pollutants, cause an increase in free radicals and lipid peroxidation.
  • Blue light emitted by the sun and by certain devices with fluorescent or D.e.l. lighting (computer, tablet, television, laptop), would have an influence on photo-aging. Its spectrum varies between 380 and 500 nm, so its rays are those among visible radiation that have the highest energy. Blue light would increase the rate of internal free radicals and the production of melanin, thus promoting the formation of pigment spots.
  • PAHs come from, among other things, the combustion of wood and organic matter as well as exhaust gases. They can settle and / or adsorb on the surface of particulate matter. PAHs can enter the skin via hair follicles or through the epidermis. They are converted into quinines, cyclic chemical compounds that produce reactive oxygen species. They can also promote acne. Among the PAHs we find 2,3,7,8-tetrachlorodibenzo-p-dioxin, a halogenated aromatic hydrocarbon, which is a lipophilic compound capable of activating the aromatic hydrocarbon receptor, which will activate the oxidative cascade (Drakaki et al. al., 2014).
  • Volatile organic compounds come from organic solvents contained in paints, varnishes, lacquers, fuels, tobacco smoke, emissions industrial and automotive. Studies have shown that these compounds induce an increase in cytokines which promote the development of inflammatory and allergic reactions such as atopic dermatitis and psoriasis. The combined action of volatile organic compounds, hydrocarbons, nitrogen oxides, and sunlight can lead to the formation of ozone (Drakaki et al., 2014).
  • Ozone can have a natural origin since it is released in small quantities by the soil and plants. However, it is mainly of anthropogenic origin. Ozone is a strong oxidizing agent capable of altering the integrity of the skin. In the skin, it induces oxidative stress and lipid peroxidation, respectively it decreases the concentration of antioxidant vitamins C and E and increases the concentration of malondialdehyde (MD A). It acts preferentially on the lipids and fatty acids constituting the lipid matrix of the horny layer of the epidermis, in which it will form reactive oxygen species. These processes will upset the balance of the epidermis and lead to multiple skin disorders via stimulation of
  • MMPs which, in turn, will degrade the collagen and elastin fibers of the dermis, accelerating skin aging, tissue sagging and ultimately deepening wrinkles (Drakaki et al., 2014).
  • the oxides are mainly nitrogen dioxide, sulfur dioxide and carbon monoxide which are involved in skin damage.
  • Nitrogen dioxide is of anthropogenic origin since it is emitted during combustion.
  • Sulfur dioxide can have the same origin as nitrogen dioxide, but also a natural origin linked to forest fires and volcanic eruptions.
  • Carbon monoxide comes from incomplete combustions. In areas where these oxides are present in high concentrations, there is an increase in atopic dermatitis in children according to studies conducted in Europe (Drakaki et al., 2014).
  • Airborne particles are fine solid particles carried by air or water. It is not the particles themselves which are responsible for the deleterious effects but the molecules adsorbed on these particles (benzo (a) pyrene, heavy metals, pesticides ). They can generate oxidative stress on the skin and are thus at least partly responsible for skin aging. They are believed to induce the formation of wrinkles and the appearance of pigment spots.
  • pollution has an effect on reducing the protective barrier function of the skin by modifying the hydrolipidic film. It will cause an alteration of the
  • patent application WO06111666 which discloses a composition containing an extract of Camellia sinensis and an extract of Lampsana communis for combating the cutaneous consequences of pollution.
  • patent FR 2838342 which relates to cosmetic compositions containing an extract of seaweed
  • Sargassum muticum useful in combating the consequences of oxidative stress induced by reactive oxygen species, ultraviolet radiation and against the effects of pollution.
  • Kostryco et al (World Scientific News 89 (2017) 90-97) describe that an extract of agrimony has anti-inflammatory properties. This document certainly mentions the anti-inflammatory properties of an agrimony extract, but such an effect is cited among many other virtues.
  • Kostryco et al thus make an inventory of the phytotherapeutic properties (point 5 page 93) of agrimony extracts and cite an anti-HBV activity, an anti-influenza activity, an antibacterial activity, an activity on the healing of wounds, an activity antioxidant which they bind to an anti-inflammatory activity and in particular at the level of the gastrointestinal mucosa, an anti -diarrheal activity, an anti-cancer activity.
  • This document cites thus mixes the weakness of the organism, epigastric pain, intestinal inflammation, hepatic disorders, digestive, enteric and gastric disorders but also cholecystitis, flatulence, varicose veins, damaged skin, baths mouth, pharyngitis, respiratory inflammation, asthma, menopausal disorders, protection of cardiovascular disorders, gynecological disorders, diabetes-related problems and pathologies of the urinary sphere.
  • This document therefore focuses on digestive inflammation but does not mention dermatological or cosmetic effects.
  • the skin cells, their lipids and proteins, but also their DNA are the targets of pollutants, which generate oxidation and inflammation.
  • Dull, dry, reactive and / or sensitive skin, acne, premature and accelerated aging, are, among others, the identified consequences of pollution.
  • This pollution therefore leads to an overproduction of free radicals in the skin, which disrupt the skin's balance and accelerate aging.
  • UV rays or tobacco pollution is therefore an additional risk factor.
  • the skin in particular the upper layer of the epidermis, constitutes a barrier against external aggressions such as chemical, mechanical or infectious and against environmental factors (climate, ultraviolet rays, pollutants and / or xenobiotics).
  • This property is mainly provided by the outermost layer of the epidermis, namely the horny layer.
  • stratum corneum One of the functions of the stratum corneum is to absorb and retain the water contained in the epidermis, and any alteration in its structure and / or function can lead to changes in the hydration of the skin and more particularly an increase in water loss. Excessive dry skin can, through a vicious circle, lead to impaired skin barrier function, and increased stress damage. environmental.
  • a skin barrier disorder can be generally defined as a condition in which the skin, or more particularly the epidermis and in particular the stratum corneum, does not exhibit a normal barrier function. This deterioration of the skin may simply be a minor aesthetic alteration and / or a sensation of skin discomfort or skin dryness. Thus, despite a few options currently available, consumers are in need of new compounds or compositions useful in combating the harmful effects of pollution on the skin, skin tone, skin texture and skin firmness as well as on the hair.
  • an extract of agrimony in particular of its aerial parts, more particularly the flowering tops, made it possible to protect the skin and integuments as well as keratin materials against the harmful effects of pollution. (example 2).
  • the invention therefore relates to a new use of an agrimony extract as an anti-pollution cosmetic agent, as well as the use of anti-pollution cosmetic compositions containing this extract.
  • the invention is aimed at a cosmetic, non-therapeutic use, as an agent for preventing or treating the effects of atmospheric pollutants on the skin and / or integuments, of an extract of agrimony, in particular an extract of aerial parts. agrimony, more particularly flowering tops, said extract being in a cosmetic composition further comprising a cosmetically acceptable excipient.
  • the invention thus relates to a non-therapeutic cosmetic use of an effective amount of an extract of agrimony, in particular an extract of aerial parts of agrimony, more particularly flowering tops, for preventing or treating the effects of atmospheric pollutants. on the skin and / or integuments.
  • the effects of air pollutants on the skin can be selected from sagging skin, gray complexion, clogged pores, loss of radiance.
  • the effects of atmospheric pollutants on the skin appendages can be chosen from dull hair, dry hair, brittle hair, oily hair.
  • the invention also relates to the use of an extract of agrimony, in particular an extract of aerial parts of agrimony, more particularly the flowering tops, in a cosmetic composition, for preventing or treating the effects of atmospheric pollutants at the level of the skin and / or integuments.
  • the invention relates to a composition or an extract according to the invention for its use to prevent or treat the effects of pollutants
  • the invention is aimed at a method for preventing or treating the effects of atmospheric pollutants on the skin and / or integuments. comprising the topical application of a composition according to the invention.
  • the invention relates to a method for preventing or treating the effects of atmospheric pollutants on the skin and / or integuments comprising the oral administration of a composition according to the invention.
  • the invention also relates to the use of an extract according to the invention for the manufacture of a cosmetic or dermatological composition intended for the treatment or prevention of the effects of atmospheric pollutants on the skin and / or integuments.
  • said cosmetic or dermatological composition is a composition suitable for topical application.
  • said cosmetic or dermatological composition is a composition suitable for oral administration.
  • the skin and / or integuments is skin or integuments exposed to an urban environment.
  • an urban environment is particularly loaded with polluting agents and a skin or integuments thus exposed require a treatment such as that of the invention.
  • the invention therefore also relates to a cosmetic method aimed at preventing or treating the effects of atmospheric pollutants on the skin and / or integuments of a person, in particular the skin of the face, said method comprising the topical application of a cosmetic composition comprising an extract of agrimony, in particular an extract of aerial parts of agrimony.
  • the person to whom the cosmetic composition is applied is a person whose skin and / or integuments, in particular the skin of the face, is or are exposed to an urban environment.
  • an extract of agrimony according to the invention in particular of its aerial parts, more particularly the flowering tops, exhibits a significant protective effect on human skin cells. , in particular the barrier layer, against several common air pollutants.
  • an extract of agrimony according to the invention in particular of its aerial parts, more particularly the flowering tops, can be used for application to healthy skin subjected or which may be subjected to the influence. environmental aggressions such as climatic imbalances or pollutants and as such likely to cause skin discomfort or skin dryness.
  • the extract of agrimony according to the invention, in particular of its aerial parts, more particularly the flowering tops can be applied to the skin when it already shows clinical signs of a deficiency of the skin barrier.
  • extract of agrimony according to the invention in particular of its aerial parts, more particularly the flowering tops, to combat damage to the human skin barrier caused by environmental attacks.
  • the present invention relates to an extract of agrimony according to the invention, in particular of its aerial parts, more particularly the flowering tops, for use in the treatment and / or prevention of disorders of the human skin barrier. and associated signs.
  • the present invention relates to the use of agrimony extract according to the invention, in particular of its aerial parts, more particularly the flowering tops, for the preparation of a composition intended to fight against attacks of the human skin barrier caused by environmental attacks and / or to prevent and / or treat skin barrier disorders and associated signs.
  • the present invention relates to a method for countering damage to the human skin barrier caused by attacks.
  • the method comprising administering to the subject in need of an effective amount of agrimony extract according to the invention, in particular its aerial parts, more particularly the flowering tops.
  • the present invention relates to a method for the treatment and / or prevention of disorders of the human skin barrier and associated signs, said method comprising administering to the subject in need of an effective amount of an extract.
  • agrimony according to the invention in particular of its aerial parts, more particularly the flowering tops.
  • Another aspect of the invention relates to a cosmetic composition or
  • composition intended to fight against damage to the human skin barrier caused by environmental attacks, said composition comprising an effective amount of agrimony extract according to the invention, in particular of its aerial parts, more particularly the flowering tops, in a cosmetically or dermocosmetically acceptable medium.
  • the extract of agrimony according to the invention offers protection against environmental attacks, in particular environmental attacks unrelated to UV, whether from the sun or from the sun. from man-made sources (including UVA and UVB rays), which can affect human skin barrier function and cause skin dryness.
  • the agrimony extract according to the invention in particular of its aerial parts, more particularly the flowering tops, or one of its compositions according to the invention can in general be intended to improve the general condition of human skin, and in particular to counter environmental attacks and to prevent and / or treat disorders of the human skin barrier and the associated signs.
  • an extract of agrimony according to the invention in particular of its aerial parts, more particularly the flowering tops, or one of its compositions is intended to prevent, treat and / or reduce the discomfort, the phenomena unpleasant or skin dryness of human skin subjected to the influence of environmental aggressions such as pollution, in particular environmental aggressions unrelated to UV.
  • an extract of agrimony according to the invention in particular of its aerial parts, more particularly the flowering tops, or one of its compositions is intended to prevent, treat and / or improve a disorder of the human skin barrier and the associated signs, the skin barrier disorder being chosen from dry skin.
  • Disorders of the human skin barrier and associated signs include all changes in the external appearance of the skin due in particular to the action of environmental attacks, in particular environmental attacks unrelated to UV rays, and to dehydration of the skin. skin (especially the epidermis), such as the rough and scaly appearance, and reduced flexibility, but also the skin sensations or discomfort caused, such as itching and / or tightness.
  • An extract of agrimony according to the invention in particular of its aerial parts, more particularly the flowering tops, or one of its compositions according to the invention may in particular be intended for preventing, treating and / or improving skin dryness and / or skin disorders associated with a state of skin dryness, in particular dryness of an epidermis, and more particularly a lack of hydration of the horny layer.
  • the genus Agrimonia includes 12 to 15 species, including the eupatory agrimony (Agrimonia eupatoria L.) which is a species of perennial nitrophilous, rhizomatous herbaceous plants, of the Rosaceae family, common throughout Europe.
  • eupatory agrimony Agrimonia eupatoria L.
  • Rosaceae family common throughout Europe.
  • the fruits are hollowed out in strong furrows throughout their length and their hooked spines are not turned back.
  • the fruit consists of 1 or 2 achenes enclosed in the bristling and persistent calyx whose throat has completely closed. It is a perennial plant, common in cool places, woods or wasteland.
  • This species is not found on the mountains at great altitudes, common in general throughout France, it is found in all Europe except in the northern part of the Scandinavian peninsula and in the arctic regions, it is also found in the northern and western regions of Asia, North Africa, the Canary Islands.
  • the plant is harvested throughout the summer, gathered into bouquets that are dried in the shade on racks or ropes.
  • agrimony is used as a mild astringent internally and externally, used in pharyngitis, gastroenteritis and intestinal disorders.
  • agrimony is used in cholecystopathies.
  • the flowering tops are traditionally used in the symptomatic treatment of mild diarrhea (oral route), subjective manifestations of venous insufficiency (heavy legs) and in hemorrhoidal symptoms (oral and local route), mouthwashes for hygiene buccal.
  • Agrimony contains phenolic compounds, volatile compounds and triterpene compounds.
  • condensed tannins accompanied by small amounts of ellagitannins and traces of gallotannins in the stems, silica, flavonoids (luteolin, apigenin heterosides and kaempferol).
  • the plant contains dimeric procyanidols (B-1, B-3, B-6, B-7), trimers (C-1, C-2, and others).
  • Agrimony extract is intended to denote the product of extraction of all or part of the Agrimonia eupatoria plant, preferably its flowering tops.
  • extraction product is meant the product obtained after extraction of aerial parts of the Agrimonia eupatoria plant, and in particular the flowering tops of Agrimonia eupatoria, with a solvent, called extraction solvent, (that is, ie a liquid solution in the extraction solvent) optionally in a concentrated or dry form after partial or total evaporation of the extraction solvent. It can therefore be a dry extract.
  • extraction solvent that is, ie a liquid solution in the extraction solvent
  • dry extract is intended to mean an extract free of extraction solvent or containing it only in the state of insignificant trace.
  • a dry extract thus contains only material derived from Agrimonia eupatoria, in particular from its flowering tops. It may also contain insignificant traces of extraction solvent. It can be combined with a drying medium such as maltodextrins, starches, silicas ...
  • the extract of Agrimonia eupatoria is advantageously obtained from the flowering tops of the plant.
  • the parts of the agrimony plant in particular its flowering tops, can be fresh, frozen or dry, whole; cut or crushed then subjected to an extraction step.
  • a method for preparing an extract according to the invention comprises a step of extracting Agrimonia eupatoria, in particular its flowering tops, with a solvent extraction.
  • the extraction can be carried out at ambient temperature (for example between 15 and 30 ° C, in particular between 20 and 25 ° C) or hot (between 40 and 250 ° C and / or at atmospheric pressure or at high pressure.
  • the extraction solvent can be a solvent chosen from:
  • glycols for example propylene glycol, butylene glycol, pentylene glycol
  • ketones including acetone and methyl ethyl acetone
  • the extraction solvent is a hydro-alcoholic solvent, that is to say a mixture of C1 to C5 alcohol / water, such as an ethanol / water mixture.
  • the solvent when it is hydroalcoholic, it may be a mixture of C1-C5 alcohol / water in an alcohol / water volume ratio of between 10/90 and 90/10, preferably between 20/80 and 40/60 .
  • the extraction solvent is an ethanol / water mixture in a volume ratio of the order of 10/90 to 90/10, more particularly between 20/80 and 40/60. More particularly still, the extraction solvent is an ethanol / water mixture in a volume ratio of 30/70.
  • the extraction can be carried out by various technologies, in particular “green” technologies, such as at atmospheric pressure (possibly using means of assistance for the extraction such as ultrasound, microwaves, leaching, maceration. , decoction ”) or at high pressures, with extraction solvents in the super- or sub-critical state such as C02 with or without co-solvent such as ethanol or water, and / or at temperatures between 20 ° C and 250 ° C.
  • green technologies such as at atmospheric pressure (possibly using means of assistance for the extraction such as ultrasound, microwaves, leaching, maceration. , decoction ”) or at high pressures, with extraction solvents in the super- or sub-critical state such as C02 with or without co-solvent such as ethanol or water, and / or at temperatures between 20 ° C and 250 ° C.
  • the solvent / parts of agrimony ratio can be between 1/5 and 1/20, particularly between 1/8 and 1/12, by mass (g) / volume (ml).
  • the extract of agrimony in particular an extract of its flowering tops, will advantageously be composed of a phenolic fraction.
  • phenolic fraction is meant a fraction of the extract containing compounds having one or more phenol groups such as flavonoids and tannins.
  • the invention relates to the use of an extract of aerial part of agrimony as an anti-pollution cosmetic agent.
  • anti-pollution cosmetic agent an ingredient of a cosmetic composition having the effect of preventing or treating the effects of atmospheric pollutants on the skin and / or integuments.
  • the present invention relates to a composition
  • dermatological or cosmetic comprising at least one extract of aerial part of agrimony with at least one dermatologically or cosmetically acceptable excipient for its use to prevent or treat the effects of atmospheric pollutants on the skin and / or integuments.
  • integuments is understood to mean the hair, body hair, nails or any other keratin material. Preferably, it is the hair.
  • the extract included in the dermatological or cosmetic composition is as described above.
  • the dermatological or cosmetic composition comprising at least one extract of aerial part of agrimony as described above and at least one dermatologically or cosmetically acceptable excipient comprises from 0.01 to 10% by weight , preferably 0.05 to 5% by weight, more preferably 0.1 to 3% by weight, more preferably 0.5 to 2% extract of aerial parts of agrimony, by weight of dry extract relative to the total weight of the composition.
  • the invention is preferably aimed at a dermatological or cosmetic composition according to the invention which is provided in a clean form and suitable for topical application, in particular to the skin, scalp and / or hair.
  • the cosmetic or dermatological composition according to the invention can thus be provided in the forms which are usually known for topical administration, that is to say in particular lotions, shampoos, balms, foams, gels, dispersions. , emulsions, sprays, serums, masks or creams, with excipients capable in particular of penetration in order to improve the properties and accessibility of the active principle.
  • it will be a milk, a cream or a balm.
  • the invention is thus aimed at dermatological or cosmetic compositions according to one of the embodiments of the present invention, characterized in that they are provided in a clean form suitable for topical application.
  • compositions generally contain, in addition to an extract of agrimony according to the invention, in particular an extract of aerial parts of agrimony, a physiologically acceptable medium, in particular one or more cosmetically or dermatologically acceptable excipients, generally based on water. or solvent, for example alcohols, ethers or glycols. They can thus also contain surfactants, complexing agents, preservatives, stabilizers, emulsifiers, thickeners, gelling agents, humectants, emollients, trace elements, essential oils, perfumes, dyes, mattifying agents, chemical or mineral filters, hydrating agents or thermal waters, anti-UV agents or sun filters, for example.
  • the cosmetic or dermatological composition according to the invention may also contain at least one other anti-pollution compound chosen from sun filters,
  • the present invention also relates to a composition
  • a composition comprising an extract of aerial parts of agrimony as described, in a form suitable for oral administration.
  • the invention thus relates to the use of an anti-polluting cosmetic composition according to the invention, characterized in that the composition is intended for oral administration.
  • the invention relates to the use of an anti-polluting cosmetic composition comprising at least one extract of aerial parts of agrimony, with at least one pharmaceutically acceptable excipient, for preventing or treating the effects of atmospheric pollutants at the level of the skin and / or integuments characterized in that the composition is intended for oral administration.
  • it may be a capsule, gelatin capsule, tablet or drinkable powder containing the extract of aerial parts of agrimony as described here in combination with usual pharmaceutically acceptable excipients such as bulking agents, stabilizers, coloring agents, traditionally used in the galenic formulation of oral pharmaceutical forms.
  • oral forms may comprise 0.01 to 10% by weight, preferably 0.05 to 5% by weight, more preferably 0.1 to 3% by weight, more preferably 0.5 to 2%. extract of aerial parts of agrimony, by weight of dry extract relative to the total weight of the composition.
  • the cosmetic or dermatological composition in a form suitable for topical application or oral administration, according to the invention, may also contain at least one other plant extract.
  • the yield is 12 g / 100 g of flowering tops.
  • the objective of this study is to evaluate the anti-oxidant, detoxifying and protective activity against ozone of an extract of agrimony, following exposure to ozone within a reconstructed human epithelium (RHE). thus mimicking the effect of pollution on the skin.
  • RHE human epithelium
  • Lipids playing a role in the barrier function of the epidermis can be impacted under stress conditions with increased oxidative damage to lipids.
  • Direct measurements of lipid peroxidation can be performed by measuring malondialdhédyde (MD A) by gas chromatography techniques combined with mass spectrometry.
  • MD A malondialdhédyde
  • Trolox 3,4-Dihydro-6-hydroxy-2,5,7,8-tetramethyl-2H-1 -benzopyran-2-carboxylic acid, or Trolox, is a hydrophilic analogue of vitamin E. It is 'an antioxidant, which is used to limit damage due to oxidative stress.
  • the agrimony extract is dissolved in water / DMSO, (80/20, v / v).
  • the process is based on lipid oxidation from ozonolysis.
  • Ozone production is carried out at 0.2 L / min by a mixture of 02 and 03 (99/1% (v / v)) in the medium.
  • the production of 1% ozone produced by this process is equivalent to 4 ppm of exposure to ozone.
  • the ozonolysis reaction induces various products called primary or secondary products according to the Criegee rearrangement.
  • RHEs are constructed from normal human primary keratinocytes obtained from subjects who have undergone abdominal dermolipectomy. After 8 days of exposure to air, the medium is changed, the test compound is added to the desired concentration. On day 9, the RHEs are placed in a chamber with a new medium. The reaction medium is cooled to 5 ° C. and under bubbled air for 1 minute. The air production is 0.2 L / min at
  • the RHEs are introduced into a plate and a new medium (without active ingredient) is added. On day 10, the RHE and media are recovered.
  • a protection index is calculated from the stress and control conditions.
  • IP (%) (stress condition - (stress condition + active)) / (stress condition - control condition) * 100.
  • the statistical evaluation is based on a one-way ANOVA followed by the Bonferroni multiple comparison test.
  • MDA ng / mg of protein
  • the inventors demonstrate that an extract of agrimony induces a protective effect against lipid peroxidation due to ozone, demonstrating an anti-pollution effect.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Dermatology (AREA)
  • Biotechnology (AREA)
  • Engineering & Computer Science (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Cosmetics (AREA)
  • Medicines Containing Plant Substances (AREA)
EP20722610.1A 2019-05-07 2020-05-06 Odermennigextrakt als umweltfreundliches mittel Pending EP3965728A1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1904763A FR3095760B1 (fr) 2019-05-07 2019-05-07 Extrait d’aigremoine comme agent anti-pollution
PCT/EP2020/062606 WO2020225318A1 (fr) 2019-05-07 2020-05-06 Extrait d'aigremoine comme agent anti-pollution

Publications (1)

Publication Number Publication Date
EP3965728A1 true EP3965728A1 (de) 2022-03-16

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EP20722610.1A Pending EP3965728A1 (de) 2019-05-07 2020-05-06 Odermennigextrakt als umweltfreundliches mittel

Country Status (8)

Country Link
US (1) US20220226231A1 (de)
EP (1) EP3965728A1 (de)
JP (1) JP2022532559A (de)
KR (1) KR20220005505A (de)
CN (1) CN114025740A (de)
FR (1) FR3095760B1 (de)
MX (1) MX2021013455A (de)
WO (1) WO2020225318A1 (de)

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FR2884718B1 (fr) 2005-04-22 2007-06-22 Clarins Soc Par Actions Simpli Composition cosmetique pour lutter contre les consequences cutanees de la pollution
CN101766555A (zh) * 2008-12-31 2010-07-07 云南恒冠实业有限公司 一种抗衰老、去皱抗皱化妆品组合物
CN102743313B (zh) * 2012-07-27 2013-06-05 王恩瀚 具有延缓皮肤衰老作用的天然药物组合物及其用途
CN104107154A (zh) * 2014-07-30 2014-10-22 广州丹奇日用化工厂有限公司 一种化妆品组合物及其制备方法
CN104434628B (zh) * 2014-11-11 2017-07-14 广东幸美化妆品股份有限公司 一种48小时持续补水的水份霜膜及其制备方法
CN104856901B (zh) * 2015-05-20 2018-09-21 广东幸美化妆品股份有限公司 一种八倍水保湿焕颜面膜及其制备方法
CN108815034A (zh) * 2018-07-29 2018-11-16 佛山文森特知识产权服务有限公司 一种细致毛孔的化妆品组合物

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US20220226231A1 (en) 2022-07-21
KR20220005505A (ko) 2022-01-13
WO2020225318A1 (fr) 2020-11-12
CN114025740A (zh) 2022-02-08
FR3095760A1 (fr) 2020-11-13
MX2021013455A (es) 2022-01-24
JP2022532559A (ja) 2022-07-15

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