EP3902920A1 - Procédé de conversion de flux contenant de l'amidon (résiduels) en protéines de haute qualité - Google Patents

Procédé de conversion de flux contenant de l'amidon (résiduels) en protéines de haute qualité

Info

Publication number
EP3902920A1
EP3902920A1 EP19839685.5A EP19839685A EP3902920A1 EP 3902920 A1 EP3902920 A1 EP 3902920A1 EP 19839685 A EP19839685 A EP 19839685A EP 3902920 A1 EP3902920 A1 EP 3902920A1
Authority
EP
European Patent Office
Prior art keywords
culture
starch
protein
residual
microbial mass
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP19839685.5A
Other languages
German (de)
English (en)
Inventor
Willy Verstraete
Mariane Adèle Leona Anna WAMBEKE
Kim Frans Marie-Louise WINDEY
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Avecom Nv
Original Assignee
Avecom Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Avecom Nv filed Critical Avecom Nv
Publication of EP3902920A1 publication Critical patent/EP3902920A1/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/40—Feeding-stuffs specially adapted for particular animals for carnivorous animals, e.g. cats or dogs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/58—Reaction vessels connected in series or in parallel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/36—Adaptation or attenuation of cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/341—Consortia of bacteria

Definitions

  • the present invention relates to a method for the conversion of starch-containing (residual) streams to high-quality proteins.
  • the invention is intended for the conversion of (residual) streams which can arise for example at the processing of potatoes to high-quality proteins.
  • starch-containing (residual) streams arise such as potato peelings, process water, cutting water and potato scraps.
  • the aim of the present invention is to convert said low-quality starch-containing (residual) streams into high-quality protein-rich products having a higher nutritional value.
  • the invention relates to a method for converting starch-containing (residual) streams of for example potatoes into high-quality proteins, in which these (residual) streams are converted by means of a two-step fermentation process, namely a first and anaerobic fermentation in which starch is converted to intermediary products such as (fatty) acids, alcohols, sugars and oligomers such as oligosaccharides and a second, but aerobic fermentation in which bacteria and yeasts are used, preferably in a single cell protein reactor or fermentor for obtaining a protein-rich reaction product.
  • a two-step fermentation process namely a first and anaerobic fermentation in which starch is converted to intermediary products such as (fatty) acids, alcohols, sugars and oligomers such as oligosaccharides and a second, but aerobic fermentation in which bacteria and yeasts are used, preferably in a single cell protein reactor or fermentor for obtaining a protein-rich reaction product.
  • the invention relates to a method for the conversion of starch-containing (residual) streams into high-quality proteins, in which the method comprises the steps:
  • the microbial mass will be separated, for example by centrifugation, from the obtained reaction product.
  • the obtained microbial mass will be sterilized, for example by a heat treatment, freeze-drying or spray-drying, in which living cells are killed in the mass. In this way, it is guaranteed that the microbial mass does not have any detrimental effects at storage and at possible applications in food and feed.
  • the microbial mass can be used as a (semi)-liquid such as a slurry.
  • the mass can further be dried so that the residual liquid is removed.
  • the obtained (sterilized) microbial mass can be used as fish feed, cattle feed, pet food or in food for human consumption.
  • the naturally active micro-organism culture from the starch-containing (residual) stream itself is used.
  • These present natural micro-organisms in this case coming from potato peelings, can be applied in other starch-rich sources.
  • a predefined culture of micro-organisms is used of which it is known that they can convert starch-containing (residual) streams.
  • micro-organisms means both bacteria and yeasts.
  • the used cultures are a mixture of at least two or more different types of micro organisms.
  • the second culture of micro-organisms will be predetermined or predefined, in which it is checked if it is suitable for converting the reaction product of the first and anaerobic fermentation.
  • the second culture will be capable of converting this reaction product to a protein concentrate of 50-80% of protein based on dry matter in this second, aerobic fermentation.
  • the reaction product of the first and anaerobic fermentation with resulting products such as (fatty) acids, sugars and oligosaccharides is separated into dissolved parts being further processed in the conversion process and undissolved parts such as peelings which are separated.
  • the separation can be realized by means of conventional means, known on the market. Non-limiting examples thereof are one or more filters, or centrifuges, or sieves, or sedimentors, or cyclones. These undissolved parts can possibly further be washed for separating the still resulting starch parts from the undissolved parts. In this way, washed potato peelings are obtained.
  • the dissolved parts will be heated shortly in a pasteurisation step.
  • the mass of living microbial parts can be reduced.
  • the reduction can be at least 4 to 6 log values, preferably for example from log 10-15 per litre to log 6-11 per litre, or for example from log 10- 15 to log 4-9 per litre.
  • the pasteurized microbial mass can be brought into a single cell protein reactor (SCPR) or fermentor, in which an aerobic fermentation is realized by means of a culture of micro-organisms which is suitable for converting the reaction product of the first and anaerobic fermentation to single cell protein or other products of added value, such as exopolysaccharides, PHA, ... by means of the second, but now aerobic fermentation in the SCPR.
  • SCPR single cell protein reactor
  • inorganic and/or organic nitrogen whether or not with phosphate, and/or micronutrients, and/or other nutrients (Ca, Mg, Fe, ...) can be added additionally in the reaction mixture of the aerobic fermentation without exceeding their maximum useful limit.
  • the definition of a culture of micro-organisms which is suitable for converting the reaction product of the first and anaerobic fermentation takes place according to a specific method with the following steps: - the definition of the microbiome by means of an aerobic fermentation on the starting substrate; - the culture of the microbiome by upscaling batches in which the strains are first cultured in small volumes, and at sufficient cell density, optic density or OD value, are fully added to a larger volume and this in several steps until a sufficiently large volume has been obtained which is representative of an industrial inoculation or more (for example between 0.1 and 10% of the reactor volume, or for example 400 ml can be representative), suitable for batch and preferably a SCTR process or Continuous Stirred Tank Reactor on laboratory scale;
  • the stability will be maintained over the production period of preferably several days to months and this by imposing selective process conditions such as pH, temperature, dilution speed, nutrient limitation, ... Stability means that the delivered product always falls within the normal range of the imposed composition and functionality; - the associated composition of a defined culture in a unique way for each substrate which is suitable for the second and aerobic fermentation of the concerned starch-containing (residual) product; - possible present yeasts are only eliminated when they are detrimental for the defined culture.
  • a culture which is suitable for the second and aerobic fermentation of potato peelings can for example have the following composition :
  • Lactobacillus (casei / paracasei)
  • Thermomonas (fusca /haemolytica / koreensis)
  • Pseudoxanthomonas (kalamensis / helianthi / wuyuanensis)
  • the remaining relatively pure peelings will also be able to be used as a covering material in the cultivation of plants and as a covering material in stables.
  • the reduced presence of starch makes is more easily to extract functional potato pectin from the peelings.
  • Desired by-products can be vitamins belonging to the B group for example and in particular (fatty) acids, but they can also be a desired amino acid profile. For example, certain strains make amino acids while other strain consume these amino acids and convert them to cellular protein fragments, as a result of which the protein content increases.
  • the stability of the whole process is guaranteed by the CSTR or continuous stirred reaction arrangement which is continuously stirred and fed.
  • the same amount of influent is fed as the amount of effluent which is discharged, in which the residence time for each process must be sufficient for maintaining the composition in the rector and of the effluent equal with a positive effect of the buffering microbial mass, the mixing culture enhancing resistance, synergy, natural adaptation and plasmid expression.
  • the two- step fermentation enhances the synergy between the two fermentors.
  • figure 1 schematically shows a device for the application of the method of the invention described in claim 1.
  • a device for the application of the method described in claim 1 for the conversion of starch-containing (residual) streams to high-quality proteins.
  • This device comprises a storage tank 1, in which in this case, a (residual) stream 2 of potato peelings and other potato rests have been collected via feed line 3. From the storage tank 1, the (residual) stream 2 is, by means of a pump 4 and a feed line 5, brought into a first fermentor 6 for anaerobic fermentation, provided with a stirring means 7.
  • the fermentation product 8 is led by means of a pump 9 via the drain line 10 to a filter 11 separating the undissolved parts 12 and peelings from the dissolved parts 13.
  • the undissolved parts 12 are drained via a transport system for further processing.
  • the dissolved parts 13 are transported by pasteurisation unit 14 for reducing the living microbial mass, for example from log 10-15 per litre to log 6-11 per litre, or for example from log 10-15 per litre to log 4-9 per litre.
  • the pasteurized microbial mass 15 is transported to a second fermentor 16, provided with a stirring means 17, but now for an aerobic fermentation.
  • a defined culture of micro-organisms 18 is added via a feed line 19 to the aerobic fermentation mixture 20.
  • the aerobic fermentation converts the pasteurized microbial mass 15 of the dissolved parts 13 of the fermentation product 8 of the first and anaerobic fermentation into single cell protein 21 which is transported via feed line 22 to a centrifugal unit 23, where, by centrifugation or any other separation means known in the state of the art, a protein concentrate 24 of 50-80% of dry matter is separated.
  • the protein concentrate 24 is sterilized by a heat treatment and dried before being used in fish feed, cattle feed, pet food or in food for human consumption.
  • the resulting liquid in the centrifugal unit 23 is led back to the first fermentor 6 via a return line 25 for anaerobic fermentation to be recycled.
  • a starch-containing (residual) product is brought into a first fermentor, in which a first and anaerobic fermentation is realized converting the insoluble and soluble starch components into intermediary products such as (fatty) acids, sugars, alcohols and oligomers such as oligosaccharides.
  • intermediary products such as (fatty) acids, sugars, alcohols and oligomers such as oligosaccharides.
  • the naturally active micro organisms from the starch-containing (residual) stream are used here.
  • the resulting fermentation product is separated by means of a filter into a dissolved fraction and an undissolved rest fraction.
  • the dissolved fraction is pasteurized by a short heating and is subsequently added to a second fermentor, in which a second, but now aerobic fermentation is realized.
  • a (predefined) culture of micro-organisms is added to the fermentor, which was selected for giving an optimal second and aerobic fermentation for the conversion of the reaction product from the first and anaerobic fermentation into single cell protein with formation of high-quality proteins in the second and aerobic fermentation.
  • This culture can also comprise yeasts, next to bacterial strains.
  • a protein concentrate 24 with a protein content of 50 to 80% on dry matter and a dry matter content of 7 to 30% of dry matter is separated by means of centrifugation, which protein concentrate 24 still is sterilized by freeze-drying, spray-drying or by a heat treatment and is dried before being used in protein-rich fish feed, cattle feed, pet food or in food for human consumption.
  • the present invention also relates to a microbial mass comprising at least 60% of protein, and further characterized by a digestibility of at least 80% and an amino acid pattern which comprises minimum the following essential amino acids expressed on raw protein: methionine > 1.5%, threonine > 4.5%, tryptophan > 1.5%, valine > 5%, alanine > 7% and glycine > 5%.
  • the protein content and the amino acid composition can be determined by known methods from the state of the art.
  • this microbial mass is obtained according to a method as described above.
  • the obtained microbial mass has a high nutritional value and is suitable for use in the food industry and feed industry.
  • the microbial mass is particularly useful for being used as fish feed, cattle feed, pet food or in food for human consumption.
  • the microbial mass can be processed in a feed or food additive, at a concentration of 0.5 to 88.9% in the additive.
  • the microbial mass can be added in liquid or semi-liquid, as well as in dry form.
  • the present invention also provides a method for providing a protein-rich component in feed such as fish feed, cattle feed, pet food or to food for human consumption; the method comprising the production of a microbial mass according to a method as described above and the addition of the microbial mass or a component comprising the microbial mass in feed and/or food.
  • the present invention also relates to a device for the conversion of starch- containing (residual) streams to high-quality proteins, characterized in that the device comprises at least two serially connected fermentors, of which a first one serves for an anaerobic fermentation of which the fermentation product undergoes an aerobic fermentation in the second fermenter with formation of high-quality protein, the device being suitable for a continuous or semi-continuous process.
  • the present invention is not limited to the embodiment described as an example and shown in the figures, but the method and device for converting starch- containing (residual) streams to high-quality proteins can be realized according to different variants without falling out of the framework of the invention, as defined in the following claims.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Polymers & Plastics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Animal Husbandry (AREA)
  • Virology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Birds (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Physiology (AREA)
  • Clinical Laboratory Science (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fodder In General (AREA)

Abstract

L'invention concerne un procédé de conversion de flux contenant de l'amidon (résiduels) en protéines de haute qualité, au moyen des étapes suivantes : -conversion des produits (résiduels) contenant de l'amidon (2) par une première fermentation anaérobie (6), avec formation d'acides gras, de sucres et d'oligosaccharides; -conversion du produit de réaction de la première fermentation anaérobie (6) en un concentré de protéines (24) par une seconde fermentation, cette fois une fermentation aérobie (20), au moyen d'une culture de micro-organismes (18); -séparation éventuelle de la masse microbienne à partir du produit de réaction obtenu (21), ladite masse comprenant un concentré de fragments de protéine cellulaire (24) destiné à être utilisé comme aliment pour poissons, aliment pour bétail, aliment pour animaux de compagnie ou dans des aliments pour une consommation humaine.
EP19839685.5A 2018-12-26 2019-12-26 Procédé de conversion de flux contenant de l'amidon (résiduels) en protéines de haute qualité Pending EP3902920A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BE20185947A BE1026952B1 (nl) 2018-12-26 2018-12-26 Werkwijze voor het omzetten van zetmeelhoudende reststromen naar hoogwaardige proteïnes
PCT/IB2019/061373 WO2020136600A1 (fr) 2018-12-26 2019-12-26 Procédé de conversion de flux contenant de l'amidon (résiduels) en protéines de haute qualité

Publications (1)

Publication Number Publication Date
EP3902920A1 true EP3902920A1 (fr) 2021-11-03

Family

ID=65200483

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19839685.5A Pending EP3902920A1 (fr) 2018-12-26 2019-12-26 Procédé de conversion de flux contenant de l'amidon (résiduels) en protéines de haute qualité

Country Status (4)

Country Link
EP (1) EP3902920A1 (fr)
BE (1) BE1026952B1 (fr)
NL (1) NL2024582B1 (fr)
WO (1) WO2020136600A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109234188B (zh) * 2018-08-15 2021-09-28 李晓明 一株异养硝化好氧反硝化菌l2及其应用
CN112746013A (zh) * 2020-12-31 2021-05-04 广东旺大集团股份有限公司 一种饲料发酵设备及其使用方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101220498B1 (ko) * 2011-02-28 2013-01-10 한국과학기술원 유기산을 원료로 하여 미생물의 체내산물을 생산하는 방법
CN106035985A (zh) * 2016-05-31 2016-10-26 同济大学 一种混菌液态发酵黄酒加工废弃物生产单细胞蛋白的方法

Also Published As

Publication number Publication date
BE1026952B1 (nl) 2020-08-04
BE1026952A1 (nl) 2020-07-30
WO2020136600A1 (fr) 2020-07-02
NL2024582B1 (nl) 2020-07-23

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