EP3902920A1 - Method for converting starch-containing (residual) streams into high-quality proteins - Google Patents
Method for converting starch-containing (residual) streams into high-quality proteinsInfo
- Publication number
- EP3902920A1 EP3902920A1 EP19839685.5A EP19839685A EP3902920A1 EP 3902920 A1 EP3902920 A1 EP 3902920A1 EP 19839685 A EP19839685 A EP 19839685A EP 3902920 A1 EP3902920 A1 EP 3902920A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- culture
- starch
- protein
- residual
- microbial mass
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/40—Feeding-stuffs specially adapted for particular animals for carnivorous animals, e.g. cats or dogs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
Abstract
A method for the conversion of starch -containing (residual) streams to high- quality proteins, by means of the following steps: - the conversion of the starch- containing (residual) products (2) in a first and anaerobic fermentation (6), with formation of fatty acids, sugars and oligosaccharides; - the conversion of the reaction product of the first and anaerobic fermentation (6) into a protein concentrate (24) in a second, but now aerobic fermentation (20), by means of a culture of micro-organisms (18); - the optional separation of the microbial mass from the obtained reaction product (21), which mass comprises a concentrate of cellular protein fragments (24) for use as fish feed, cattle feed, pet food or in food for human consumption.
Description
METHOD FOR CONVERTING STARCH-CONTAINING (RESIDUAL) STREAMS INTO HIGH-QUALITY PROTEINS
The present invention relates to a method for the conversion of starch-containing (residual) streams to high-quality proteins.
More in particular, the invention is intended for the conversion of (residual) streams which can arise for example at the processing of potatoes to high-quality proteins.
It is known that, at the processing of potatoes, starch-containing (residual) streams arise such as potato peelings, process water, cutting water and potato scraps.
Traditionally, these (residual) streams are not further processed except as low- quality feed or as additives for the agriculture.
The aim of the present invention is to convert said low-quality starch-containing (residual) streams into high-quality protein-rich products having a higher nutritional value.
Thereto, the invention relates to a method for converting starch-containing (residual) streams of for example potatoes into high-quality proteins, in which these (residual) streams are converted by means of a two-step fermentation process, namely a first and anaerobic fermentation in which starch is converted to intermediary products such as (fatty) acids, alcohols, sugars and oligomers such as oligosaccharides and a second, but aerobic fermentation in which bacteria and yeasts are used, preferably in a single cell protein reactor or fermentor for obtaining a protein-rich reaction product.
Thereto, the invention relates to a method for the conversion of starch-containing (residual) streams into high-quality proteins, in which the method comprises the steps:
- the conversion of the starch-containing (residual) products by means of a first and anaerobic fermentation, converting the insoluble and soluble starch
components into intermediary products such as (fatty) acids, alcohols, sugars and oligomers such as oligosaccharides;
- the conversion of the reaction product of the first and anaerobic fermentation into a protein concentrate by means of a second, but aerobic fermentation, by using a culture of micro-organisms which can comprise, next to bacterial strains, also yeasts, in which a microbial mass is obtained, comprising a concentrate of cellular protein fragments.
In an embodiment, the microbial mass will be separated, for example by centrifugation, from the obtained reaction product.
In a further or other embodiment, the obtained microbial mass will be sterilized, for example by a heat treatment, freeze-drying or spray-drying, in which living cells are killed in the mass. In this way, it is guaranteed that the microbial mass does not have any detrimental effects at storage and at possible applications in food and feed.
In an embodiment, the microbial mass can be used as a (semi)-liquid such as a slurry. In another embodiment, the mass can further be dried so that the residual liquid is removed.
The obtained (sterilized) microbial mass can be used as fish feed, cattle feed, pet food or in food for human consumption.
In an embodiment, for the first and anaerobic fermentation, the naturally active micro-organism culture from the starch-containing (residual) stream itself is used. These present natural micro-organisms, in this case coming from potato peelings, can be applied in other starch-rich sources.
In another embodiment, a predefined culture of micro-organisms is used of which it is known that they can convert starch-containing (residual) streams. The term "micro-organisms" means both bacteria and yeasts. In a preferred embodiment, the used cultures are a mixture of at least two or more different types of micro organisms.
In an embodiment, the second culture of micro-organisms will be predetermined or predefined, in which it is checked if it is suitable for converting the reaction product of the first and anaerobic fermentation. In a preferred embodiment, the second culture will be capable of converting this reaction product to a protein concentrate of 50-80% of protein based on dry matter in this second, aerobic fermentation.
In an embodiment, the reaction product of the first and anaerobic fermentation with resulting products such as (fatty) acids, sugars and oligosaccharides is separated into dissolved parts being further processed in the conversion process and undissolved parts such as peelings which are separated. The separation can be realized by means of conventional means, known on the market. Non-limiting examples thereof are one or more filters, or centrifuges, or sieves, or sedimentors, or cyclones. These undissolved parts can possibly further be washed for separating the still resulting starch parts from the undissolved parts. In this way, washed potato peelings are obtained.
In an embodiment, the dissolved parts will be heated shortly in a pasteurisation step. As a result, the mass of living microbial parts can be reduced. In an embodiment, the reduction can be at least 4 to 6 log values, preferably for example from log 10-15 per litre to log 6-11 per litre, or for example from log 10- 15 to log 4-9 per litre. Subsequently, the pasteurized microbial mass can be brought into a single cell protein reactor (SCPR) or fermentor, in which an aerobic fermentation is realized by means of a culture of micro-organisms which is suitable for converting the reaction product of the first and anaerobic fermentation to single cell protein or other products of added value, such as exopolysaccharides, PHA, ... by means of the second, but now aerobic fermentation in the SCPR.
In an embodiment, inorganic and/or organic nitrogen, whether or not with phosphate, and/or micronutrients, and/or other nutrients (Ca, Mg, Fe, ...) can be added additionally in the reaction mixture of the aerobic fermentation without exceeding their maximum useful limit.
The definition of a culture of micro-organisms which is suitable for converting the reaction product of the first and anaerobic fermentation takes place according to a specific method with the following steps:
- the definition of the microbiome by means of an aerobic fermentation on the starting substrate; - the culture of the microbiome by upscaling batches in which the strains are first cultured in small volumes, and at sufficient cell density, optic density or OD value, are fully added to a larger volume and this in several steps until a sufficiently large volume has been obtained which is representative of an industrial inoculation or more (for example between 0.1 and 10% of the reactor volume, or for example 400 ml can be representative), suitable for batch and preferably a SCTR process or Continuous Stirred Tank Reactor on laboratory scale;
- the gradual additional addition of starting substrate, in which the same amount of mass is also discharged gradually from the reactor, as a result of which the mass in the reactor obtains the same composition as the outflowing mass;
- the plating of the obtained culture on selective growth media after adaptation and obtaining a protein content of 50-80% of the dry matter; - the identification of the isolated strains by means of DNA sequencing;
- the quantification of the defined strains;
- the elimination of the identified strains which are known to be detrimental for the defined culture of micro-organisms, in which the mixed culture is modified as little as possible for maintaining the earlier obtained functionality and stability as much as possible.
In a preferred embodiment, the stability will be maintained over the production period of preferably several days to months and this by imposing selective process conditions such as pH, temperature, dilution speed, nutrient limitation, ... Stability means that the delivered product always falls within the normal range of the imposed composition and functionality; - the associated composition of a defined culture in a unique way for each substrate which is suitable for the second and aerobic fermentation of the concerned starch-containing (residual) product;
- possible present yeasts are only eliminated when they are detrimental for the defined culture. A culture which is suitable for the second and aerobic fermentation of potato peelings can for example have the following composition :
Castellaniella (denitrificans / defragrans / daejeonensis)
Lactobacillus (casei / paracasei)
Lactobacillus fermentum
Xenophilus (unidentified / azovorans)
Paracoccus (denitrificans / pantotrophus)
Thermomonas (fusca /haemolytica / koreensis)
Acinetobacter venetianus
Pigmentiphaga (kullae / daeguensis)
Stenotrophomonas(unidentified/nitritireducens/ acidaminiphila)
Hydrogenophaga (unidentified / temperata)
Pseudoxanthomonas (jiangsuensis / uncult.)
Pseudoxanthomonas (kalamensis / helianthi / wuyuanensis)
Pichia kudriavzevii
It is obvious that other cultures can also be suitable for this purpose. Once an suitably defined culture is known, it can obviously be re-used repeatedly for the same starch-containing (residual) stream, without having to make a new selection. The defined culture will in time further adapt to the substrate, in which the included strains do not get lost, but become more robust against unfavourable influences.
For a new type of starch-containing (residual) streams, a new selection can be made according to the same method.
Next to the valorisation of the microbial product, the remaining relatively pure peelings will also be able to be used as a covering material in the cultivation of plants and as a covering material in stables. Also, the reduced presence of starch makes is more easily to extract functional potato pectin from the peelings.
By openly selecting the microbiome, the composition of the culture can still be further driven in the direction of desired by-products next to the obtained protein. Desired by-products can be vitamins belonging to the B group for example and in particular (fatty) acids, but they can also be a desired amino acid profile. For example, certain strains make amino acids while other strain consume these amino acids and convert them to cellular protein fragments, as a result of which the protein content increases.
On the one hand, the stability of the whole process is guaranteed by the CSTR or continuous stirred reaction arrangement which is continuously stirred and fed. Hereby, the same amount of influent is fed as the amount of effluent which is discharged, in which the residence time for each process must be sufficient for maintaining the composition in the rector and of the effluent equal with a positive effect of the buffering microbial mass, the mixing culture enhancing resistance, synergy, natural adaptation and plasmid expression. On the other hand, the two- step fermentation enhances the synergy between the two fermentors.
Considering the characteristics of the invention, in the following a non-limiting example of a preferred application of the method for the conversion of starch- containing (residual) streams to high-quality proteins according to the invention will be described, with reference to the associated figures, in which: figure 1 schematically shows a device for the application of the method of the invention described in claim 1.
In figure 1, a device is shown for the application of the method described in claim 1 for the conversion of starch-containing (residual) streams to high-quality proteins. This device comprises a storage tank 1, in which in this case, a (residual) stream 2 of potato peelings and other potato rests have been collected via feed line 3. From the storage tank 1, the (residual) stream 2 is, by means of a pump 4 and a feed line 5, brought into a first fermentor 6 for anaerobic fermentation, provided with a stirring means 7.
After the anaerobic fermentation, the fermentation product 8 is led by means of a pump 9 via the drain line 10 to a filter 11 separating the undissolved parts 12 and peelings from the dissolved parts 13. The undissolved parts 12 are drained via a transport system for further processing. The dissolved parts 13 are transported by
pasteurisation unit 14 for reducing the living microbial mass, for example from log 10-15 per litre to log 6-11 per litre, or for example from log 10-15 per litre to log 4-9 per litre. Subsequently, the pasteurized microbial mass 15 is transported to a second fermentor 16, provided with a stirring means 17, but now for an aerobic fermentation. Thereto, a defined culture of micro-organisms 18 is added via a feed line 19 to the aerobic fermentation mixture 20.
The aerobic fermentation converts the pasteurized microbial mass 15 of the dissolved parts 13 of the fermentation product 8 of the first and anaerobic fermentation into single cell protein 21 which is transported via feed line 22 to a centrifugal unit 23, where, by centrifugation or any other separation means known in the state of the art, a protein concentrate 24 of 50-80% of dry matter is separated. The protein concentrate 24 is sterilized by a heat treatment and dried before being used in fish feed, cattle feed, pet food or in food for human consumption.
The resulting liquid in the centrifugal unit 23 is led back to the first fermentor 6 via a return line 25 for anaerobic fermentation to be recycled.
The operation of the device shown in figure 1 is very simple and is as follows.
A starch-containing (residual) product is brought into a first fermentor, in which a first and anaerobic fermentation is realized converting the insoluble and soluble starch components into intermediary products such as (fatty) acids, sugars, alcohols and oligomers such as oligosaccharides. The naturally active micro organisms from the starch-containing (residual) stream are used here.
The resulting fermentation product is separated by means of a filter into a dissolved fraction and an undissolved rest fraction. The dissolved fraction is pasteurized by a short heating and is subsequently added to a second fermentor, in which a second, but now aerobic fermentation is realized.
Thereto, a (predefined) culture of micro-organisms is added to the fermentor, which was selected for giving an optimal second and aerobic fermentation for the conversion of the reaction product from the first and anaerobic fermentation into single cell protein with formation of high-quality proteins in the second and aerobic fermentation. This culture can also comprise yeasts, next to bacterial strains.
By using CSTR reactors, the feed of substrate and the discharge of product is slowly optimized to the aimed residence times. This means that the predefined culture should not always be administered repeatedly.
From the fermentation product of the second and aerobic fermentation, a protein concentrate 24 with a protein content of 50 to 80% on dry matter and a dry matter content of 7 to 30% of dry matter is separated by means of centrifugation, which protein concentrate 24 still is sterilized by freeze-drying, spray-drying or by a heat treatment and is dried before being used in protein-rich fish feed, cattle feed, pet food or in food for human consumption.
The present invention also relates to a microbial mass comprising at least 60% of protein, and further characterized by a digestibility of at least 80% and an amino acid pattern which comprises minimum the following essential amino acids expressed on raw protein: methionine > 1.5%, threonine > 4.5%, tryptophan > 1.5%, valine > 5%, alanine > 7% and glycine > 5%.
The protein content and the amino acid composition can be determined by known methods from the state of the art.
In a preferred embodiment, this microbial mass is obtained according to a method as described above.
The obtained microbial mass has a high nutritional value and is suitable for use in the food industry and feed industry. The microbial mass is particularly useful for being used as fish feed, cattle feed, pet food or in food for human consumption. Thereto, the microbial mass can be processed in a feed or food additive, at a concentration of 0.5 to 88.9% in the additive. The microbial mass can be added in liquid or semi-liquid, as well as in dry form.
Consequently, the present invention also provides a method for providing a protein-rich component in feed such as fish feed, cattle feed, pet food or to food for human consumption; the method comprising the production of a microbial mass according to a method as described above and the addition of the microbial mass or a component comprising the microbial mass in feed and/or food.
The present invention also relates to a device for the conversion of starch- containing (residual) streams to high-quality proteins, characterized in that the device comprises at least two serially connected fermentors, of which a first one serves for an anaerobic fermentation of which the fermentation product undergoes an aerobic fermentation in the second fermenter with formation of high-quality protein, the device being suitable for a continuous or semi-continuous process.
The present invention is not limited to the embodiment described as an example and shown in the figures, but the method and device for converting starch- containing (residual) streams to high-quality proteins can be realized according to different variants without falling out of the framework of the invention, as defined in the following claims.
Claims
1. A method for the conversion of starch-containing (residual) streams to high- quality proteins, characterized by the steps:
- the conversion of starch-containing (residual) products (2) by means of a first and anaerobic fermentation (6), converting the insoluble and soluble starch components into intermediary products;
- the conversion of the reaction product of the first and anaerobic fermentation (6) into a protein concentrate (24) by means of a second, but aerobic fermentation (20), by using a culture of micro-organisms (18) which can comprise, next to bacterial strains, also yeasts, suitable for converting the reaction product of the first, anaerobic fermentation;
- optionally the separation of the microbial mass (23) from the obtained reaction product (21), which microbial mass comprises a concentrate of cellular protein fragments (24);
- optionally the sterilisation of the obtained microbial mass (23) by a heat treatment, freeze-drying or spray-drying process in which living cells are killed.
2. The method according to claim 1, characterized in that, for the first and anaerobic fermentation (6), the naturally active micro-organism culture from the starch-containing (residual) stream (2) itself is used.
3. The method according to any one of the previous claims, characterized in that the second culture of micro-organisms, used for the aerobic fermentation, is predefined, in which it is determined if the culture is suitable for converting the reaction product of the first, anaerobic fermentation to a protein concentrate of 50 to 80% of proteins based on dry matter.
4. The method according to any one of the previous claims, characterized in that the reaction product of the first and anaerobic fermentation (6) is separated into dissolved parts (13) being further processed in the conversion process and undissolved parts (15) which are separated.
5. The method according to claim 4, characterized in that the undissolved parts (15) are further washed for separating the still remaining starch parts from the undissolved parts (15).
6. The method according to claim 4 or 5, characterized in that the dissolved parts (13) are shortly heated in a pasteurisation (14) step for reducing the living microbial mass, preferably from log 10-15 per litre to log 6-11 per litre, or from log 10-15 per litre to log 4-9 per litre.
7. The method according to claim 6, characterized in that the dissolved parts (13) with the pasteurized microbial mass are brought into a single cell protein reactor (SCPR), in which an aerobic fermentation is realized by means of a defined culture of micro-organisms (18) which is suitable for converting the reaction product (8) of the first and anaerobic fermentation (6) into single cell protein by means of the second, but now aerobic fermentation in the SCPR or single cell protein reactor (20).
8. The method according to claim 7, characterized in that the inorganic and/or organic nitrogen and/or phosphate and/or micro(nutrients) are added to the reaction mixture of the aerobic fermentation (20) without exceeding their maximum useful limit.
9. The method according to claim 43, characterized in that the definition of a culture of micro-organisms (18) which is suitable for converting the reaction product (8) of the first and anaerobic fermentation (6) takes place according to a specific method with the following steps:
- defining of the microbiome by means of an aerobic fermentation on the starting substrate;
- culturing of the microbiome by upscaling batches in which the strains are first cultured in small volumes, and at sufficient cell density, optic density or OD value, are fully added to a larger volume and this in several steps until a sufficiently large volume has been obtained, which is representative for an industrial inoculation or more, suitable for batch and preferably a SCTR process or Continuous Stirred Tank Reactor on laboratory scale;
- the gradual additional addition of starting substrate, in which the same amount of mass is also removed gradually from the reactor, as a result of which the mass in the reactor obtains the same composition as the outflowing mass;
- the plating of the obtained culture on selective growth media after adaptation and obtaining a protein content of 50-80% of protein based on the dry matter;
- the identification of the isolated strains by means of DNA sequencing;
- the quantification of the defined strains;
- the elimination of the identified strains which are known to be detrimental for the defined culture (18) of micro-organisms, in which the mixed culture is modified as little as possible for maintaining the earlier obtained functionality and stability as much as possible.
- composing with this, a defined culture (18) in a unique for each substrate which is suitable for the second and aerobic fermentation (20) of the concerned starch- containing (residual) product (2);
- the elimination of possibly present yeasts, only if they are detrimental for the defined culture.
10. The method according to any one of the previous claims, characterized in that the culture (18) has the following composition for the second and aerobic fermentation (20) of starch-containing residual (streams) has the following composition :
Castellaniella (denitrificans / defragrans / daejeonensis)
Lactobacillus (casei / paracasei)
Lactobacillus fermentum
Xenophilus (unidentified / azovorans)
Paracoccus (denitrificans / pantotrophus)
Thermomonas (fusca /haemolytica / koreensis)
Acinetobacter venetianus
Pigmentiphaga (kullae / daeguensis)
Stenotrophomonas(unidentified/nitritireducens/ acidaminiphila)
Hydrogenophaga (unidentified / temperata)
Pseudoxanthomonas (jiangsuensis / uncult.)
Pseudoxanthomonas (kalamensis / helianthi / wuyuanensis)
Pichia kudriavzevii.
11. The method according to any one of the previous claims, characterized in that the starch-containing (residual) streams are potatoes or by-products of potatoes, such as potato peelings, process water, cutting water and potato scraps.
12. A device for the conversion of starch-containing (residual) streams to high- quality proteins, characterized in that the device comprises at least two serially connected fermentors (6, 20), of which a first one (6) serves for an anaerobic fermentation of which the fermentation product (8) undergoes an aerobic fermentation in the second fermenter (20) with formation of high-quality protein (24).
13. The device according to claim 12, characterized in that the de device is suitable for a continuous or semi-continuous process.
14. A microbial mass comprising at least 60% of protein, and further characterized by a digestibility of at least 80% and an amino acid pattern which comprises minimum the following essential amino acids expressed on raw protein : methionine > 1.5%, threonine > 4.5%, tryptophan > 1.5%, valine > 5%, alanine > 7% and glycine > 5%.
15. The microbial mass according to the previous claim, characterized in that the microbial mass is obtained according to a method of any one of the claims 1 to 11.
16. A use of a microbial mass according to any one of the claims 14 or 15 as a fish feed, cattle feed, pet food or in food for human consumption.
17. A feed or food additive, provided with 0.5 to 99.9% of a microbial mass according to any one of the claims 14 or 15.
18. A method for providing a protein-rich component in feed such as fish feed, cattle feed, pet food or to food for human consumption; the method comprising the production of a microbial mass according to any one of the claims 1 to 11 and
the addition of the microbial mass or a component comprising the microbial mass in feed and/or food.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BE20185947A BE1026952B1 (en) | 2018-12-26 | 2018-12-26 | Method for converting starch-containing residual flows into high-quality proteins |
| PCT/IB2019/061373 WO2020136600A1 (en) | 2018-12-26 | 2019-12-26 | Method for converting starch-containing (residual) streams into high-quality proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EP3902920A1 true EP3902920A1 (en) | 2021-11-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP19839685.5A Pending EP3902920A1 (en) | 2018-12-26 | 2019-12-26 | Method for converting starch-containing (residual) streams into high-quality proteins |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP3902920A1 (en) |
| BE (1) | BE1026952B1 (en) |
| NL (1) | NL2024582B1 (en) |
| WO (1) | WO2020136600A1 (en) |
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| CN109234188B (en) * | 2018-08-15 | 2021-09-28 | 李晓明 | Heterotrophic nitrification aerobic denitrifying bacterium L2 and application thereof |
| CN112746013A (en) * | 2020-12-31 | 2021-05-04 | 广东旺大集团股份有限公司 | Feed fermentation equipment and using method thereof |
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| KR101220498B1 (en) * | 2011-02-28 | 2013-01-10 | 한국과학기술원 | Method of Producing Microbial Intracellular Products from Volatile Fatty Acids |
| CN106035985A (en) * | 2016-05-31 | 2016-10-26 | 同济大学 | Method for producing single cell proteins by using processed waste from mixed bacteria liquid fermentation of yellow wine |
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2018
- 2018-12-26 BE BE20185947A patent/BE1026952B1/en active IP Right Grant
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2019
- 2019-12-26 EP EP19839685.5A patent/EP3902920A1/en active Pending
- 2019-12-26 WO PCT/IB2019/061373 patent/WO2020136600A1/en unknown
- 2019-12-26 NL NL2024582A patent/NL2024582B1/en active
Also Published As
| Publication number | Publication date |
|---|---|
| NL2024582B1 (en) | 2020-07-23 |
| BE1026952B1 (en) | 2020-08-04 |
| WO2020136600A1 (en) | 2020-07-02 |
| BE1026952A1 (en) | 2020-07-30 |
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