EP3664612A1 - Composition made from polyol(s) and sterol(s) for use in the agricultural field - Google Patents

Composition made from polyol(s) and sterol(s) for use in the agricultural field

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Publication number
EP3664612A1
EP3664612A1 EP18755875.4A EP18755875A EP3664612A1 EP 3664612 A1 EP3664612 A1 EP 3664612A1 EP 18755875 A EP18755875 A EP 18755875A EP 3664612 A1 EP3664612 A1 EP 3664612A1
Authority
EP
European Patent Office
Prior art keywords
use according
plant
sterol
polyols
nonionic surfactant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP18755875.4A
Other languages
German (de)
French (fr)
Inventor
Alexandra Fregonese
Marie NAVARRO
Alexandre EVEILLARD
Marie Laure Hisette Jourdainne
Aymeric MOLIN
Olivier GOULAY
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Elicit Plant
Original Assignee
Elicit Plant
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR1757557A external-priority patent/FR3069756B1/en
Priority claimed from FR1757558A external-priority patent/FR3069757B1/en
Application filed by Elicit Plant filed Critical Elicit Plant
Publication of EP3664612A1 publication Critical patent/EP3664612A1/en
Pending legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom

Definitions

  • plants have intrinsic means to defend themselves.
  • biotic stress denotes stress that comes from living organisms such as pathogenic microorganisms, for example fungi, bacteria, viruses, but also nematodes, insects, mites herbivores, or parasitic plants.
  • the term “elicitor” denotes a substance that is recognized by plants and activates a signaling cascade that leads the plant to mobilize its defenses.
  • An elicitor is a substance capable, under certain conditions, of stimulating natural defense mechanisms. These natural defenses are directed against bio-aggressors (diseases, pests).
  • abiotic stress denotes stress that results from the non-living such as water stress, salt stress, flooding stress, wind, heat stress ( cold, frost, heat shock), stress due to ultraviolet (solar radiation), stress related to nutrient deficiencies, stress injury, oxidative stress, osmotic stress, chemical stress.
  • bio stimulant or plant biostimulant is defined in accordance with the study commissioned by the Center for Studies and Foresight of the Ministry of Agriculture, Agri-Food and Forestry (MAAF) and financed by MAAF under Program 215 (Contract No. SSP-2013-094, Final Report - December 2014) entitled "Agricultural Stimulation Products to Improve the Biological Functionality of Soils and Plants" - Review of Available Knowledge and strategic recommendations ".
  • a biostimulant is: "A material that contains a substance (s) and / or microorganism” whose function, when applied to plants or the rhizosphere, is to stimulate natural processes to improve / benefit the nutrient absorption, nutrient efficiency, tolerance to abiotic stress, and crop quality, regardless of the nutrient content of the biostimulant. ". (EBIC, 2014).
  • biostimulants currently used are microorganisms, plant extracts or synthetic chemical compounds. However, the effectiveness of these biostimulants is not optimal.
  • the first problem to be solved by the invention is that of developing compositions that contribute to the biostimulation of plants.
  • the goal is to develop alternative compositions that are able to promote plant resistance to abiotic stress more effectively than the compounds of the prior art.
  • the goal is to stimulate certain natural mechanisms to improve / benefit nutrient absorption, nutrient efficiency, tolerance to abiotic stress, and crop quality with, in the case of nutrients in particular, for direct or indirect effect, the growth of the plant, and thus the improvement of the yield.
  • a second problem to be solved by the invention is that of developing compositions that are capable of stimulating certain natural mechanisms to improve tolerance to biotic stress.
  • a third problem to be solved by the invention is that of developing compositions that make it possible to limit the quantity applied of plant protection products while retaining the same effect as the standard doses.
  • the third objective is to develop a composition that can be used as an adjunct phytosanitary composition in particular.
  • the Applicant has found that these objectives were achieved by combining a nonionic surfactant derived from polyols and a sterol hereinafter referred to as "the composition or composition of the invention".
  • the invention firstly relates to the use of at least one nonionic surfactant derived from polyols and a sterol as bio stimulant of a plant hereinafter referred to as "the composition or composition of the invention ".
  • the composition of the invention is used to promote germination.
  • the composition of the invention is used to promote the elongation of the main roots and the multiplication and elongation of the secondary roots, in other words the growth of the main and secondary roots.
  • the composition of the invention is used to promote flowering.
  • the composition of the invention is used to promote the resistance of plants against abiotic stresses.
  • the composition is used in particular to limit the loss of stomatal water, that is to say to allow the plant to better withstand water stress.
  • the composition of the invention has a particular effect on the activation of the acquired systemic resistance system.
  • the composition acts not only to stimulate natural defense mechanisms against biotic stress but also to promote resistance to abiotic stress.
  • composition of the invention is not a product with specific activity makes it possible to envisage broad-spectrum use on a large number of crops, which can save minor crops for which the number of phytosanitary products available is almost -no.
  • the Applicant has found that the composition of the invention makes it possible to improve the spreading of liquids on the sheet, in particular of an aqueous solution. This phenomenon results from the reduction of the surface tension of the sheet in contact with the composition of the invention. It follows that by improving the spreading of a solution containing an active product with respect to the plant, the necessary amount of product is reduced for an equivalent effect.
  • the invention also relates to the use of the composition as an adjuvant of a solution containing an active product vis-à-vis the plant.
  • biocontrol products are intended to prevent the action of plant pests and are chosen in particular from fungicides, fungicides, bactericides, bacteriostats, insecticides, acaricides, parasiticides, nematicides, taupicides or repellents for birds or game, one or more substances intended to destroy undesirable plants or to slow down their chosen growth, in particular among herbicides or antidicotyledons.
  • composition of the invention thus constitutes an economy for the farmer that reduces the number of applications (of field trips).
  • composition of the invention When used in combination with an active product vis-à-vis the plant as described above, it is simultaneously or sequentially.
  • This type of synergy corresponds in all respects to the demands of society in terms of phytosanitary products:
  • composition according to the invention may be applied after emergence or before emergence, on the seed, the seedling (juvenile stage prior to flowering), the plant during flowering (before, during or after pollination), the plant after fertilization, the plant being fruited, the fruit, flowers, leaves, stems, roots or soil, before or after sowing.
  • composition may be in practice applied by spraying, watering, addition to a hydroponic culture medium, immersion of the seed and / or coating of the seed.
  • the plant is chosen from the group comprising Dicotyledonous and Monocotyledonous plants, advantageously in the group comprising cereals, root and tuber crops, saccharifers, legumes, nut plants, oleaginous plants. or oleaginous plants, vegetable crops, fruit trees, aromatic plants and spices, flowering plants, or industrial crop plants for the production of a raw material for processing.
  • the nonionic surfactant derived from polyols is chosen from the group comprising sugar and fatty acid esters, alkyl-mono- and alkyl-poly-glucosides, alkyl-aliphatic esters and mono- and alkyl-poly-glucosides and fatty acids and N-alkylglucamides, especially N-methylglucamides.
  • the nonionic surfactant derived from polyols is chosen from the group comprising sucrose esters, sorbitan esters and glucose esters. According to one particular embodiment, the nonionic surfactant derived from polyols is ethoxylated or non-ethoxylated.
  • the nonionic surfactant derived from polyols is sucrose stearate.
  • the sterol is chosen from cholesterol, plant sterols such as campesterol, beta-sitosterol, stigmasterol, brassicasterol, campestanol, sitostanol, animal sterols such as lanosterol or sterols. yeasts or fungi such as ergosterol.
  • the sterol is beta-sitosterol.
  • the nonionic surfactant derived from polyols and the sterol are in solution, advantageously in the form of an aqueous solution, comprising 0.01% to 80%, preferably 0.05% to 30%, so still more preferably 0.75% to 3% by weight of the nonionic surfactant solution derived from polyols and sterol.
  • the use according to the invention of at least one nonionic surfactant derived from polyols corresponding to sucrose stearate and the sterol corresponding to beta-sitosterol is advantageousously, the use according to the invention of at least one nonionic surfactant derived from polyols corresponding to sucrose stearate and the sterol corresponding to beta-sitosterol.
  • the invention relates to a composition
  • a composition comprising sucrose stearate as a nonionic surfactant derived from polyols and, as sterol, at least beta-sistosterol or stigmasterol or cholesterol or ergosterol or a mixture thereof.
  • the beta-sistosterol is between 1 and 99% by weight of the composition and the sucrose stearate is between 99 and 1% by weight of the composition, preferably the beta-sistosterol is between 40 and 1% and the sucrose stearate is between 60 and 99% by weight of the composition.
  • Figure 1 shows the comparison of the port of parsley plants after watering with water or a solution comprising the combination of sucrose stearate and beta-sitosterol according to the invention.
  • FIG. 2 represents the quantification of salicylic acid in parsley plants 2, 4 or 8 hours after watering with water or a solution comprising the combination of sucrose stearate and beta-sitosterol according to the invention.
  • FIGS. 3 and 4 represent the effect of a control solution (water, C) or of a solution comprising 1%, 3% or 10% of the combination of sucrose stearate and beta-sitosterol according to the invention (1 %, 3% and 10%>) on the rupture of AArabidopsis thaliana seeds seeded in stratified ( Figure 3) or non-stratified ( Figure 4).
  • FIGS. 5 and 6 show the effect of a control solution (water, C) or of a solution comprising 1%, 3% or 10% of the combination of sucrose stearate and beta-sitosterol according to the invention (1 %, 3% and 10%) on the emergence of the radicle of Arabidopsis thaliana seeds grown in stratified ( Figure 5) or non-stratified ( Figure 6).
  • FIGS. 5 and 6 show the effect of a control solution (water, C) or of a solution comprising 1%, 3% or 10% of the combination of sucrose stearate and beta-sitosterol according to the invention (1 %, 3% and 10%) on the emergence of the radicle of Arabidopsis thaliana seeds grown in stratified ( Figure 5) or non-stratified ( Figure 6).
  • FIGS. 5 and 6 show the effect of a control solution (water, C) or of a solution comprising 1%, 3% or 10% of the combination of sucrose stearate and beta-si
  • FIG. 7 and 8 show the effect of a control solution (water, C) or of a solution comprising 1%, 3% or 10% of the combination of sucrose stearate and beta-sitosterol according to the invention (1 %, 3% and 10%) on the opening of cotyledons of Arabidopsis thaliana seeds grown in stratified ( Figure 7) or non-stratified ( Figure 8).
  • FIG. 9 represents the seeds of Arabidopsis thaliana germinated 120 hours after treatment with water (A), a solution comprising 1% (B) or 10% (C) of the combination of sucrose stearate and beta-sitosterol according to the invention.
  • Figure 10 shows the measurement of the length of the main root of Arabidopsis thaliana plants grown in the absence of sucrose stearate and beta-sitosterol (0%) or in the presence of 10 ⁇ 5 % or 10 ⁇ 3 % of sucrose stearate and beta-sitosterol in solution.
  • Figure 11 shows the evaluation of the secondary root system of Arabidopsis thaliana plants grown in the absence of sucrose stearate and beta-sitosterol (0%) or in the presence of 10 ⁇ 5 % or 10 ⁇ 3 % sucrose stearate. and beta-sitosterol in solution.
  • Figure 12 shows the flowering time of Arabidopsis thaliana plants treated with solutions comprising 1, 3 and 10% of the sucrose stearate and beta-sitosterol mixture or a control solution (water; H 2 O) sprayed once (1%, 3%). % and 10%) or twice at two-day intervals (1% 2X, 3% 2X and 10% 2X).
  • FIG. 13 represents the evolution of the loss in stomatal water as a function of time of plants of Arabidopsis thaliana treated with water or a solution comprising the sucrose stearate and beta-sitosterol mixture according to the invention.
  • EXAMPLE 1 Preparation of Combinations of a Nonionic Surfactant and a Sterol According to the Invention and Evaluation of Their Effect on the Resistance of Parsley (Petroselinum Crispum) to Abiotic Stress
  • Various combinations of the invention with a plant sterol are prepared from sucrose stearate and beta-sitosterol.
  • the combinations are prepared by dry blending sucrose stearate and beta-sitosterol, with proportions varying from 0 to 100% by weight relative to the total mass of the mixture, for each of these ingredients, as indicated in the following Table 1. .
  • Potted parsley plants are grown in a climatic chamber under the following conditions: 23 ° C and a photoperiod of 16h day / 8h night. Before treatment all the leaves of the parsley plants are cut. The treatment of parsley plants consists of watering the pots every three days with: - 40 ml of water (Control lot)
  • sample S produces the optimal effect of resistance of the plant to abiotic stress (sample S composed of 80% sucrose stearate and 20% beta-sitosterol).
  • Salicylic acid is a phenolic compound that is involved in both the establishment of local resistance and general resistance (SAR) in plants.
  • the application of the solutions is done by a watering at the foot of the plant (40 ml) and a spray on the leaves.
  • the tested solutions are:
  • treated batch B solution composed of 97% of water and 3% of sample Y, 100% of sucrose stearate and 0% of beta-sitosterol;
  • treated batch C solution composed of 97% of water and 3% of the sample S, ie 80% of sucrose stearate and 20% of beta-sitosterol.
  • Each batch consists of four pots.
  • sucrose stearate and beta-sitosterol induces a stimulation of salicylic acid synthesis which is greater than the addition of the effects of each individual compound (sample A: beta -sitosterol + sample Y: sucrose stearate), in response to biotic stress.
  • Arabidopsis thaliana seeds are placed in a climatic chamber under a photoperiod of 16h day / 8h night at 22 ° C.
  • the seeds were imbibed with water (control) or with the composition according to the invention at concentrations of 1%, 3% and 10%) in water.
  • the seeds were previously stratified (that is, exposed to cold) or unstratified.
  • Arabidopsis thaliana plants are cultivated on agar medium in a climatic chamber under the following conditions: 23 ° C and a photoperiod of 16h day / 8h night.
  • Standard agar medium is used as a control.
  • Agar media containing 10 ⁇ 5 % and 10 ⁇ 3 % of the sucrose stearate and beta-sitosterol mixture according to the sample S are used to evaluate the effect of the invention on the development of the root system.
  • the length of the main root and secondary roots is measured between the 2nd and 21st day post germination.
  • Figure 10 shows that the main roots of plants grown in the presence of 10 "5% and 10" 3% of the mixture according to the invention is longer than for plants grown in agar medium control.
  • Figure 11 shows that plants grown in agar media containing 10 "5% and 10" 3% of the mixture according to the invention have more lateral roots that are also longer. It follows from this demonstration that the mixture sucrose stearate and beta-sitosterol allows growth of the main and secondary root system. As a result, the plant is better anchored in the soil and the penetration of nutrients into the plant is more effective due to more extensive secondary root system.
  • Plants Arabidopsis thaliana potted are grown according to the conditions of Example 1.
  • Solutions comprising 1, 3 and 10% of the sucrose stearate and beta-sitosterol mixture according to sample S or a control solution (water, H 2 O) are sprayed once (1%, 3% and 10%) or twice at two times. days apart (1% 2X, 3% 2X and 10%> 2X), on plants 3 weeks old.
  • the number of flowering plants by modalities was measured as a function of time.
  • sucrose stearate and beta-sitosterol mixture according to the invention accelerates flowering to Arabidopsis thaliana.
  • Plants, 4 weeks old, are treated with a foliar spray, 4 hours before the measurements are made, with water (Control) or a solution comprising 3% of the mixture sucrose stearate and beta-sitosterol according to the sample S.
  • Example 7 Evaluation of the wetting effect of a non-anionic surfactant and a sterol according to the invention on Arabidopsis thaliana
  • Plants Arabidopsis thaliana potted are grown according to the conditions of Example 1.
  • the leaves with Arabidopsis thaliana are treated with a foliar spray, with water (Control) or a solution comprising 3% of the sucrose stearate and beta-sitosterol mixture according to the sample S.
  • the number of droplets present on the leaf surface of the plant is quantified.
  • the results show a decrease in the number of droplets on the Arabidopsis thaliana leaf after application of the mixture according to the invention.
  • the composition according to the invention makes it possible to reduce the surface tension of the sprayed solution on the sheets and thus allows better spreading of the droplets and better adhesion of the solution to the sheet.
  • results show that there is no accumulation of the solution comprising the mixture sucrose stearate and beta-sitosterol according to the invention in the ribs and axillary buds of the plant.
  • sucrose stearate and beta-sitosterol makes it possible in particular to treat grasses known to be poorly wettable.
  • Another advantage is the use of this mixture as an adjuvant for products that have a contact action.
  • the use of the mixture according to the invention makes it possible to increase the cover of the plant and to use less product per hectare.

Abstract

Use of at least one non-ionic surfactant derived from polyols and a sterol for promoting the growth of a plant and the resistance of a plant to an abiotic stress, and stimulating the defence systems of a plant against a biotic stress.

Description

COMPOSITION A BASE DE POLYOL(S) ET DE STEROL(S) POUR USAGE DANS LE DOMAINE AGRICOLE  COMPOSITION BASED ON POLYOL (S) AND STEROL (S) FOR USE IN THE AGRICULTURAL FIELD
Au cours de leur évolution, les végétaux ont mis en place des barrières mécaniques protectrices (cuticule, paroi pectocellulosique), notamment contre les bioagresseurs. Ces barrières leur confèrent une résistance constitutive, notamment face aux agents pathogènes. En cas de franchissement de ces barrières mécaniques, les plantes ont su développer des mécanismes de défense active comme la résistance systémique acquise (SAR). La SAR est certes moins intense ou active que l'application d'un produit phytosanitaire mais elle est durable : la plante est préparée à une nouvelle attaque du pathogène ou d'un autre agresseur et pourra y répondre plus rapidement. During their evolution, the plants have put in place protective mechanical barriers (cuticle, pectocellulosic wall), especially against pests. These barriers give them a constitutive resistance, especially in the face of pathogens. In the event of crossing these mechanical barriers, the plants have been able to develop active defense mechanisms such as acquired systemic resistance (SAR). The SAR is certainly less intense or active than the application of a phytosanitary product but it is sustainable: the plant is prepared for a new attack of the pathogen or another aggressor and can respond more quickly.
En d'autres termes, les plantes disposent de moyens intrinsèques pour se défendre. In other words, plants have intrinsic means to defend themselves.
Renforcer leurs propres moyens de défense en réponse à un stress biotique plutôt que de combattre directement l'agresseur au moyen de produits phytosanitaires constitue une solution intéressante sur les plans scientifique et agronomique. On trouve comme stimulateurs des moyens de défense, les stimulateurs des défenses naturelles des plantes (SDN), que l'on désigne également éliciteurs. Strengthening their own defenses in response to biotic stress rather than fighting the attacker directly with plant protection products is an interesting scientific and agronomic solution. As stimulators, we find defenses, the stimulators of the natural defenses of plants (SDN), which are also called elicitors.
Au sens de l'invention, par « stress biotique », on désigne un stress qui provient d'organismes vivant tel des microorganismes pathogènes par exemple des champignons, des bactéries, des virus, mais encore des nématodes, des insectes, des acariens, des herbivores, ou des plantes parasites. For the purposes of the invention, "biotic stress" denotes stress that comes from living organisms such as pathogenic microorganisms, for example fungi, bacteria, viruses, but also nematodes, insects, mites herbivores, or parasitic plants.
Au sens de l'invention, par « éliciteur », on désigne une substance qui est reconnue par les plantes et active une cascade de signalisation qui conduit la plante à mobiliser ses moyens de défense. Un éliciteur est une substance capable, dans certaines conditions, de stimuler des mécanismes de défense naturelle. Ces défenses naturelles sont dirigées contre des bio- agresseurs (maladies, ravageurs). For the purposes of the invention, the term "elicitor" denotes a substance that is recognized by plants and activates a signaling cascade that leads the plant to mobilize its defenses. An elicitor is a substance capable, under certain conditions, of stimulating natural defense mechanisms. These natural defenses are directed against bio-aggressors (diseases, pests).
On sait également rendre les plantes plus résistantes face à des stress abio tiques, par utilisation d'un biostimulant. It is also known to make plants more resistant to abio stress by using a biostimulant.
Au sens de l'invention, par « stress abiotique », on désigne un stress qui résulte du non vivant tel un stress hydrique, un stress salin, un stress inondation (« flooding »), le vent (verse), un stress thermique (froid, gel, choc thermique), un stress dû aux ultraviolets (rayonnement solaire), un stress lié aux carences nutritives, un stress blessure, un stress oxydatif, un stress osmotique, un stress chimique. Dans le cadre de la présente invention le terme de bio stimulant ou biostimulant végétal est défini conformément à l'étude commanditée par le Centre d'Études et de Prospective du Ministère de l'Agriculture, de l'Agroalimentaire et de la Forêt (MAAF) et financée par le MAAF dans le cadre du programme 215 (Marché n° SSP-2013-094, Rapport final - décembre 2014) intitulée « Produits de stimulation en agriculture visant à améliorer les fonctionnalités biologiques des sols et des plantes - Étude des connaissances disponibles et recommandations stratégiques». For the purposes of the invention, the term "abiotic stress" denotes stress that results from the non-living such as water stress, salt stress, flooding stress, wind, heat stress ( cold, frost, heat shock), stress due to ultraviolet (solar radiation), stress related to nutrient deficiencies, stress injury, oxidative stress, osmotic stress, chemical stress. In the context of the present invention the term bio stimulant or plant biostimulant is defined in accordance with the study commissioned by the Center for Studies and Foresight of the Ministry of Agriculture, Agri-Food and Forestry (MAAF) and financed by MAAF under Program 215 (Contract No. SSP-2013-094, Final Report - December 2014) entitled "Agricultural Stimulation Products to Improve the Biological Functionality of Soils and Plants" - Review of Available Knowledge and strategic recommendations ".
Un biostimulant est : « Un matériel qui contient une (des) substance(s) et/ou micro- organisme^) dont la fonction, quand appliqué aux plantes ou à la rhizosphère, est de stimuler les processus naturels pour améliorer/avantager l'absorption des nutriments, l'efficience des nutriments, la tolérance aux stress abiotiques, et la qualité des cultures, indépendamment du contenu en nutriments du biostimulant. ». (EBIC, 2014). A biostimulant is: "A material that contains a substance (s) and / or microorganism" whose function, when applied to plants or the rhizosphere, is to stimulate natural processes to improve / benefit the nutrient absorption, nutrient efficiency, tolerance to abiotic stress, and crop quality, regardless of the nutrient content of the biostimulant. ". (EBIC, 2014).
A titre d'exemple, des biostimulants actuellement mis en œuvre sont des microorganismes, des extraits de plantes ou encore des composés chimiques de synthèse. Toutefois, l'efficacité de ces biostimulants n'est pas optimale. By way of example, biostimulants currently used are microorganisms, plant extracts or synthetic chemical compounds. However, the effectiveness of these biostimulants is not optimal.
En parallèle, l'agriculture contemporaine a un besoin de plus en plus grand de protéger ses cultures et ses récoltes si elle veut maintenir ses hauts rendements et ses marges déjà faibles dans certaines productions. At the same time, contemporary agriculture has a growing need to protect its crops and crops if it wants to maintain its high yields and already low margins in some productions.
La mauvaise image des pesticides dans le public (en témoigne l'actuelle campagne de communication menée par l'Union des Industries pour la Protection des Plantes - UIPP) pose des problèmes. Par ailleurs, l'efficacité des produits phytosanitaires tend à diminuer. En effet, comme dans le cas des antibiotiques utilisés en médecine humaine, des résistances apparaissent et les rendent moins, voire inefficaces. Pour l'ensemble de ces raisons, il est nécessaire de limiter au maximum leur utilisation en optimisant au mieux leurs effets. The poor image of pesticides in the public (evidenced by the current communication campaign led by the Union of Industries for the Protection of Plants - UIPP) poses problems. In addition, the effectiveness of plant protection products tends to decrease. Indeed, as in the case of antibiotics used in human medicine, resistances appear and make them less, if not ineffective. For all these reasons, it is necessary to limit their use as much as possible by optimizing their effects.
Le premier problème que se propose de résoudre l'invention est donc celui de mettre au point des compositions qui contribuent à la biostimulation des plantes. The first problem to be solved by the invention is that of developing compositions that contribute to the biostimulation of plants.
Par conséquent, l'objectif est celui de mettre au point des compositions alternatives qui soient capables de favoriser la résistance des plantes au regard d'un stress abiotique de façon plus efficace que les composés de l'art antérieur. Therefore, the goal is to develop alternative compositions that are able to promote plant resistance to abiotic stress more effectively than the compounds of the prior art.
En d'autres termes, l'objectif est celui de stimuler certains mécanismes naturels pour améliorer /avantager l'absorption des nutriments, l'efficience des nutriments, la tolérance aux stress abiotiques, et la qualité des cultures avec, dans le cas des nutriments notamment, pour effet direct ou indirect, la croissance de la plante, et donc l'amélioration du rendement. Un second problème que se propose de résoudre l'invention est celui de mettre au point des compositions, qui soit capables de stimuler certains mécanismes naturels pour améliorer la tolérance aux stress biotiques. In other words, the goal is to stimulate certain natural mechanisms to improve / benefit nutrient absorption, nutrient efficiency, tolerance to abiotic stress, and crop quality with, in the case of nutrients in particular, for direct or indirect effect, the growth of the plant, and thus the improvement of the yield. A second problem to be solved by the invention is that of developing compositions that are capable of stimulating certain natural mechanisms to improve tolerance to biotic stress.
Un troisième problème que se propose de résoudre l'invention est celui de mettre au point des compositions qui permettent de limiter la quantité appliquée de produits phytosanitaires tout en gardant le même effet que les doses standards. En d'autres termes, le troisième objectif est de mettre au point une composition qui puisse être utilisée comme adjuvant de composition phytosanitaire notamment. A third problem to be solved by the invention is that of developing compositions that make it possible to limit the quantity applied of plant protection products while retaining the same effect as the standard doses. In other words, the third objective is to develop a composition that can be used as an adjunct phytosanitary composition in particular.
La présente invention répond à l'ensemble de ces problèmes techniques. Plus précisément, le Demandeur a constaté que ces objectifs étaient atteints en combinant un tensio actif non ionique dérivé de polyols et un stérol ci-après désigné « la composition ou la composition de l'invention ». The present invention addresses all of these technical problems. More specifically, the Applicant has found that these objectives were achieved by combining a nonionic surfactant derived from polyols and a sterol hereinafter referred to as "the composition or composition of the invention".
Par conséquent, l'invention a tout d'abord pour objet l'utilisation d'au moins un tensioactif non ionique dérivé de polyols et un stérol comme bio stimulant d'un végétal ci- après désigné « la composition ou la composition de l'invention ». Therefore, the invention firstly relates to the use of at least one nonionic surfactant derived from polyols and a sterol as bio stimulant of a plant hereinafter referred to as "the composition or composition of the invention ".
Comme premier effet contribuant à la bio stimulation, la composition de l'invention est utilisée pour favoriser la germination. As a first effect contributing to the bio stimulation, the composition of the invention is used to promote germination.
Comme second effet contribuant à la biostimulation, la composition de l'invention est utilisée pour favoriser l'allongement des racines principales et la multiplication et l'allongement des racines secondaires, autrement dit la croissance des racines principales et secondaires. As a second effect contributing to the biostimulation, the composition of the invention is used to promote the elongation of the main roots and the multiplication and elongation of the secondary roots, in other words the growth of the main and secondary roots.
Comme troisième effet contribuant à la bio stimulation, la composition de l'invention est utilisée pour favoriser la floraison. As third effect contributing to the bio stimulation, the composition of the invention is used to promote flowering.
Comme troisième effet contribuant à la bio stimulation, la composition de l'invention est utilisée pour favoriser la résistance des plantes contre des stress abiotiques. As a third effect contributing to bio stimulation, the composition of the invention is used to promote the resistance of plants against abiotic stresses.
En tant que produit améliorant la résistance au stress abiotique, la composition est utilisée notamment pour limiter la déperdition d'eau stomatique, c'est-à-dire pour permettre à la plante de mieux résister à un stress hydrique. As a product improving abiotic stress resistance, the composition is used in particular to limit the loss of stomatal water, that is to say to allow the plant to better withstand water stress.
En tant que mécanisme de défense naturelle vis-à-vis d'un stress biotique, la composition de l'invention a un effet particulier sur l'activation du système de résistance systémique acquise. Dans certains cas et selon l'invention, la composition agit non seulement pour stimuler des mécanismes de défense naturelle contre des stress biotiques mais également pour favoriser la résistance à des stress abiotiques. As a natural defense mechanism against biotic stress, the composition of the invention has a particular effect on the activation of the acquired systemic resistance system. In some cases and according to the invention, the composition acts not only to stimulate natural defense mechanisms against biotic stress but also to promote resistance to abiotic stress.
Le fait que la composition de l'invention ne soit pas un produit à activité spécifique permet d'envisager une utilisation à large spectre sur un grand nombre de cultures, ce qui peut sauver des cultures mineures pour lesquelles le nombre de produits phytosanitaires disponibles est quasi-nul. The fact that the composition of the invention is not a product with specific activity makes it possible to envisage broad-spectrum use on a large number of crops, which can save minor crops for which the number of phytosanitary products available is almost -no.
Par ailleurs, le Demandeur a constaté que la composition de l'invention permettait d'améliorer l'étalement des liquides sur la feuille, en particulier d'une solution aqueuse. Ce phénomène résulterait de la diminution de la tension de surface de la feuille au contact de la composition de l'invention. Il s'ensuit qu'en améliorant l'étalement d'une solution contenant un produit actif vis-à-vis de la plante, on diminue la quantité nécessaire de produit pour un effet équivalent. Moreover, the Applicant has found that the composition of the invention makes it possible to improve the spreading of liquids on the sheet, in particular of an aqueous solution. This phenomenon results from the reduction of the surface tension of the sheet in contact with the composition of the invention. It follows that by improving the spreading of a solution containing an active product with respect to the plant, the necessary amount of product is reduced for an equivalent effect.
Cela présente donc un intérêt particulier vis-à-vis des produits phytosanitaires. This is therefore of particular interest with regard to plant protection products.
Par conséquent et selon un autre aspect, l'invention a également pour objet l'utilisation de la composition comme adjuvant d'une solution contenant un produit actif vis-à-vis de la plante. Therefore and in another aspect, the invention also relates to the use of the composition as an adjuvant of a solution containing an active product vis-à-vis the plant.
Comme produit actif, on peut envisager notamment les nutriments, un ou plusieurs engrais, un ou plusieurs régulateurs de croissance et/ou avec un produit de biocontrôle. Les produits de biocontrôle visent à prévenir l'action d'organismes nuisibles pour les végétaux et sont choisis notamment parmi les fongicides, les fongistatiques, les bactéricides, les bactériostatiques, les insecticides, les acaricides, les parasiticides, les nématicides, les taupicides ou les répulsifs pour oiseaux ou gibiers, une ou plusieurs substances qui visent à détruire les végétaux indésirables ou à ralentir leur croissance choisie notamment parmi des herbicides ou des antidicotylédones. As an active product, it is possible to envisage in particular nutrients, one or more fertilizers, one or more growth regulators and / or with a biocontrol product. The biocontrol products are intended to prevent the action of plant pests and are chosen in particular from fungicides, fungicides, bactericides, bacteriostats, insecticides, acaricides, parasiticides, nematicides, taupicides or repellents for birds or game, one or more substances intended to destroy undesirable plants or to slow down their chosen growth, in particular among herbicides or antidicotyledons.
La composition de l'invention constitue ainsi une économie pour l'agriculteur qui réduit le nombre d'applications (de passages au champ). The composition of the invention thus constitutes an economy for the farmer that reduces the number of applications (of field trips).
Avec son mode d'action indirect, le produit n' entraine pas de résistance. Qui plus est, l'utilisation éventuellement en alternance, mais surtout en association avec des produits phytosanitaires «classiques» (produit de biocontrôle) permet non seulement, comme déjà mentionné, de limiter la quantité de produit à appliquer mais également d'éviter ou de retarder l'apparition de résistances à ces produits et augmenter leur durabilité. With its indirect mode of action, the product does not cause resistance. What is more, the use alternatively alternatively, but especially in combination with "traditional" phytosanitary products (biocontrol product) allows not only, as already mentioned, to limit the amount of product to be applied but also to avoid or delay the emergence of resistance to these products and increase their durability.
Lorsque la composition de l'invention est utilisée en association avec un produit actif vis- à-vis de la plante tel que décrit précédemment, elle l'est de façon simultanée ou séquentielle. Ce type de synergie correspond en tous points aux demandes de la société en matière de produits phytosanitaires : When the composition of the invention is used in combination with an active product vis-à-vis the plant as described above, it is simultaneously or sequentially. This type of synergy corresponds in all respects to the demands of society in terms of phytosanitary products:
- Respect de l'environnement ;  - Respect the environment ;
- Absence de danger pour l'homme ;  - Absence of danger for the man;
- Faibles doses ;  - Low doses;
- Large spectre d'utilisation ;  - Wide spectrum of use;
- Multiculture ;  - Multiculture;
- Pas de résistance induite ;  - No induced resistance;
- Aide à retarder l'apparition de résistance aux produits phytosanitaires ;  - Helps to delay the appearance of resistance to phytosanitary products;
- Diminution des intrants ;  - Reduction of inputs;
- Amélioration des conditions environnementales ;  - Improvement of environmental conditions;
- Intérêt économique ;  - Economic interest;
- Intérêt réglementaire.  - Regulatory interest.
La composition selon l'invention peut être appliquée après l'émergence ou avant l'émergence, sur la graine, la plantule (stade juvénile antérieur à la floraison), la plante en cours de floraison (avant, pendant ou après pollinisation), la plante après fécondation, la plante en cours de fructification, le fruit, les fleurs, les feuilles, les tiges, les racines ou dans le sol, avant ou après semis. The composition according to the invention may be applied after emergence or before emergence, on the seed, the seedling (juvenile stage prior to flowering), the plant during flowering (before, during or after pollination), the plant after fertilization, the plant being fruited, the fruit, flowers, leaves, stems, roots or soil, before or after sowing.
La composition peut être en pratique appliquée par pulvérisation, arrosage, addition à un milieu de culture en hydroponie, immersion de la graine et/ou enrobage de la graine. The composition may be in practice applied by spraying, watering, addition to a hydroponic culture medium, immersion of the seed and / or coating of the seed.
Il est possible de traiter des végétaux cultivés en plein champ ou des végétaux sous serre ou des végétaux cultivés en hors sol. It is possible to treat plants grown in the open field or greenhouse plants or plants grown above ground.
Selon l'invention, le végétal est choisi dans le groupe comprenant les Dicotylédones et les Monocotylédones, avantageusement dans le groupe comprenant les céréales, les plantes à racines et tubercules, les saccharifères, les légumineuses, les végétaux à fruits à coques, les plantes oléifères ou oléagineuses, les plantes de culture légumière, les fruitiers, les plantes aromatiques et les épices, les plantes de cultures florales, ou encore les plantes de culture industrielle destinée à la production d'une matière première en vue de sa transformation. According to the invention, the plant is chosen from the group comprising Dicotyledonous and Monocotyledonous plants, advantageously in the group comprising cereals, root and tuber crops, saccharifers, legumes, nut plants, oleaginous plants. or oleaginous plants, vegetable crops, fruit trees, aromatic plants and spices, flowering plants, or industrial crop plants for the production of a raw material for processing.
Selon une autre caractéristique de l'invention, le tensioactif non ionique dérivé de polyols est choisi dans le groupe comprenant les esters de sucre et d'acides gras, les alkyl-mono- et alkyl- poly-glucosides, les esters d' alkyl-mono- et alkyl-poly-glucosides et d'acides gras et les N- alkylglucamides, en particulier les N-methylglucamides. According to another characteristic of the invention, the nonionic surfactant derived from polyols is chosen from the group comprising sugar and fatty acid esters, alkyl-mono- and alkyl-poly-glucosides, alkyl-aliphatic esters and mono- and alkyl-poly-glucosides and fatty acids and N-alkylglucamides, especially N-methylglucamides.
Avantageusement, le tensioactif non ionique dérivé de polyols est choisi dans le groupe comprenant les esters de saccharose, les esters de sorbitan et les esters de glucose. Selon un mode de réalisation particulier, le tensioactif non ionique dérivé de polyols est éthoxylé ou non éthoxylé. Advantageously, the nonionic surfactant derived from polyols is chosen from the group comprising sucrose esters, sorbitan esters and glucose esters. According to one particular embodiment, the nonionic surfactant derived from polyols is ethoxylated or non-ethoxylated.
De manière avantageuse, le tensioactif non ionique dérivé de polyols est le stéarate du saccharose. Advantageously, the nonionic surfactant derived from polyols is sucrose stearate.
Selon une autre caractéristique de l'invention, le stérol est choisi parmi le cholestérol, les stérols végétaux comme le campestérol, le béta-sitostérol, le stigmastérol, le brassicastérol, le campestanol, le sitostanol, les stérols animaux comme le lanostérol ou les stérols de levures ou champignons comme l'ergostérol. According to another characteristic of the invention, the sterol is chosen from cholesterol, plant sterols such as campesterol, beta-sitosterol, stigmasterol, brassicasterol, campestanol, sitostanol, animal sterols such as lanosterol or sterols. yeasts or fungi such as ergosterol.
Avantageusement, le stérol est le béta-sitostérol. Advantageously, the sterol is beta-sitosterol.
Selon un mode de réalisation particulier, le tensioactif non ionique dérivé de polyols et le stérol sont en solution, avantageusement sous la forme d'une solution aqueuse, comprenant 0.01% à 80%, de préférence 0.05%> à 30%>, de façon encore plus préférentielle 0.75%> à 3% en poids de la solution de tensioactif non ionique dérivé de polyols et de stérol. According to one particular embodiment, the nonionic surfactant derived from polyols and the sterol are in solution, advantageously in the form of an aqueous solution, comprising 0.01% to 80%, preferably 0.05% to 30%, so still more preferably 0.75% to 3% by weight of the nonionic surfactant solution derived from polyols and sterol.
Avantageusement, l'utilisation selon l'invention d'au moins un tensioactif non ionique dérivé de polyols correspondant au stéarate du saccharose et le stérol correspondant au béta- sitostérol. Advantageously, the use according to the invention of at least one nonionic surfactant derived from polyols corresponding to sucrose stearate and the sterol corresponding to beta-sitosterol.
Selon un autre aspect, l'invention concerne une composition comprenant le stéarate de saccharose comme tensioactif non ionique dérivé des polyols et, en tant que stérol, au moins le béta-sistostérol ou le stigmastérol ou le cholestérol ou l'ergostérol ou leur mélange. In another aspect, the invention relates to a composition comprising sucrose stearate as a nonionic surfactant derived from polyols and, as sterol, at least beta-sistosterol or stigmasterol or cholesterol or ergosterol or a mixture thereof.
Avantageusement, le béta-sistostérol est compris entre 1 et 99 % en poids de la composition et le stéarate de saccharose est compris entre 99 et 1% en poids de la composition, de préférence le béta-sistostérol est compris entre 40 et 1 % et le stéarate de saccharose est compris entre 60 et 99 % en poids de la composition. Advantageously, the beta-sistosterol is between 1 and 99% by weight of the composition and the sucrose stearate is between 99 and 1% by weight of the composition, preferably the beta-sistosterol is between 40 and 1% and the sucrose stearate is between 60 and 99% by weight of the composition.
La manière dont l'invention peut être réalisée et les avantages qui en découlent ressortiront mieux des exemples de réalisation qui suivent, donnés à titre indicatif et non limitatif, à l'appui des figures annexées. The way in which the invention can be realized and the advantages which result therefrom will emerge more clearly from the following exemplary embodiments, given by way of non-limiting indication, in support of the appended figures.
La figure 1 représente la comparaison du port de plants de persil après arrosage avec de l'eau ou une solution comprenant la combinaison de sucrose stéarate et de béta-sitostérol selon l'invention. Figure 1 shows the comparison of the port of parsley plants after watering with water or a solution comprising the combination of sucrose stearate and beta-sitosterol according to the invention.
La figure 2 représente la quantification de l'acide salicylique dans des plants de persil 2, 4 ou 8 heures après arrosage avec de l'eau ou une solution comprenant la combinaison de sucrose stéarate et de béta-sitostérol selon l'invention. FIG. 2 represents the quantification of salicylic acid in parsley plants 2, 4 or 8 hours after watering with water or a solution comprising the combination of sucrose stearate and beta-sitosterol according to the invention.
Les figures 3 et 4 représentent l'effet d'une solution contrôle (eau ; C) ou d'une solution comprenant 1%, 3% ou 10% de la combinaison de sucrose stéarate et de béta-sitostérol selon l'invention (1%, 3% et 10%>) sur la rupture des téguments de graines AArabidopsis thaliana cultivées en milieux stratifié (Figure 3) ou non stratifié (Figure 4). FIGS. 3 and 4 represent the effect of a control solution (water, C) or of a solution comprising 1%, 3% or 10% of the combination of sucrose stearate and beta-sitosterol according to the invention (1 %, 3% and 10%>) on the rupture of AArabidopsis thaliana seeds seeded in stratified (Figure 3) or non-stratified (Figure 4).
Les figures 5 et 6 représentent l'effet d'une solution contrôle (eau ; C) ou d'une solution comprenant 1%, 3% ou 10% de la combinaison de sucrose stéarate et de béta-sitostérol selon l'invention (1%, 3% et 10%) sur l'émergence de la radicule de graines d'Arabidopsis thaliana cultivées en milieux stratifié (Figure 5) ou non stratifié (Figure 6). Les figures 7 et 8 représentent l'effet d'une solution contrôle (eau ; C) ou d'une solution comprenant 1%, 3% ou 10% de la combinaison de sucrose stéarate et de béta-sitostérol selon l'invention (1%, 3% et 10%) sur l'ouverture des cotylédons de graines d'Arabidopsis thaliana cultivées en milieux stratifié (Figure 7) ou non stratifié (Figure 8). FIGS. 5 and 6 show the effect of a control solution (water, C) or of a solution comprising 1%, 3% or 10% of the combination of sucrose stearate and beta-sitosterol according to the invention (1 %, 3% and 10%) on the emergence of the radicle of Arabidopsis thaliana seeds grown in stratified (Figure 5) or non-stratified (Figure 6). FIGS. 7 and 8 show the effect of a control solution (water, C) or of a solution comprising 1%, 3% or 10% of the combination of sucrose stearate and beta-sitosterol according to the invention (1 %, 3% and 10%) on the opening of cotyledons of Arabidopsis thaliana seeds grown in stratified (Figure 7) or non-stratified (Figure 8).
La figure 9 représente les graines d'Arabidopsis thaliana germées 120 heures après traitement par de l'eau (A), une solution comprenant 1% (B) ou 10% (C) de la combinaison de sucrose stéarate et de béta-sitostérol selon l'invention. FIG. 9 represents the seeds of Arabidopsis thaliana germinated 120 hours after treatment with water (A), a solution comprising 1% (B) or 10% (C) of the combination of sucrose stearate and beta-sitosterol according to the invention.
La figure 10 représente la mesure de la longueur de la racine principale de plants d'Arabidopsis thaliana cultivés en l'absence de sucrose stéarate et de béta-sitostérol (0%) ou en présence de 10~5% ou 10~3% de sucrose stéarate et de béta-sitostérol en solution. La figure 11 représente l'évaluation du système racinaire secondaire de plants d'Arabidopsis thaliana cultivés en l'absence de sucrose stéarate et de béta-sitostérol (0%) ou en présence de 10~5% ou 10~3% de sucrose stéarate et de béta-sitostérol en solution. Figure 10 shows the measurement of the length of the main root of Arabidopsis thaliana plants grown in the absence of sucrose stearate and beta-sitosterol (0%) or in the presence of 10 ~ 5 % or 10 ~ 3 % of sucrose stearate and beta-sitosterol in solution. Figure 11 shows the evaluation of the secondary root system of Arabidopsis thaliana plants grown in the absence of sucrose stearate and beta-sitosterol (0%) or in the presence of 10 ~ 5 % or 10 ~ 3 % sucrose stearate. and beta-sitosterol in solution.
La figure 12 représente le temps de floraison de plants d'Arabidopsis thaliana traités avec des solutions comprenant 1, 3 et 10% du mélange sucrose stéarate et béta-sitostérol ou une solution contrôle (eau ; H20) pulvérisés une fois (1%, 3% et 10%) ou deux fois à deux jours d'intervalle (1% 2X, 3% 2X et 10% 2X). Figure 12 shows the flowering time of Arabidopsis thaliana plants treated with solutions comprising 1, 3 and 10% of the sucrose stearate and beta-sitosterol mixture or a control solution (water; H 2 O) sprayed once (1%, 3%). % and 10%) or twice at two-day intervals (1% 2X, 3% 2X and 10% 2X).
La figure 13 représente l'évolution de la déperdition en eau stomatique en fonction du temps de plants d'Arabidopsis thaliana traités avec de l'eau ou une solution comprenant le mélange sucrose stéarate et béta-sitostérol selon l'invention. Exemple 1 : Préparation de combinaisons d'un tensioactif non ionique et d'un stérol selon l'invention et évaluation de leur effet sur la résistance du persil (Petroselinum crispum) à un stress abiotique Différentes combinaisons de l'invention avec un stérol végétal sont préparées à partir de sucrose stéarate et de béta-sitostérol. FIG. 13 represents the evolution of the loss in stomatal water as a function of time of plants of Arabidopsis thaliana treated with water or a solution comprising the sucrose stearate and beta-sitosterol mixture according to the invention. EXAMPLE 1 Preparation of Combinations of a Nonionic Surfactant and a Sterol According to the Invention and Evaluation of Their Effect on the Resistance of Parsley (Petroselinum Crispum) to Abiotic Stress Various combinations of the invention with a plant sterol are prepared from sucrose stearate and beta-sitosterol.
Les combinaisons sont préparées par mélange à sec de sucrose stéarate et de béta- sitostérol, avec des proportions variant de 0 à 100% en masse par rapport à la masse totale du mélange, pour chacun de ces ingrédients, comme indiqué dans le tableau 1 suivant. The combinations are prepared by dry blending sucrose stearate and beta-sitosterol, with proportions varying from 0 to 100% by weight relative to the total mass of the mixture, for each of these ingredients, as indicated in the following Table 1. .
Tableau 1 Table 1
L'efficacité de chaque combinaison sur la tolérance des plantes au stress abiotique est analysée. The effectiveness of each combination on plant tolerance to abiotic stress is analyzed.
Des plants de persil en pot sont cultivés dans une enceinte climatique sous les conditions suivantes: 23°C et une photopériode de 16h de jour/8h de nuit. Avant le traitement l'ensemble des feuilles des plants de persil sont coupées. Le traitement des plants de persil consiste à arroser les pots tous les trois jours avec : - 40 ml d'eau (lot Contrôle) Potted parsley plants are grown in a climatic chamber under the following conditions: 23 ° C and a photoperiod of 16h day / 8h night. Before treatment all the leaves of the parsley plants are cut. The treatment of parsley plants consists of watering the pots every three days with: - 40 ml of water (Control lot)
- 40 ml d'une solution composée d'un échantillon selon le tableau 1 (97% d'eau + 3% de l'échantillon) - 40 ml of a solution composed of a sample according to Table 1 (97% water + 3% of the sample)
Cet arrosage abondant a pour but de mimer un stress de type « inondation» (flooding). Chaque lot est constitué de quatre pots. This abundant watering is intended to mimic a flooding type of stress. Each batch consists of four pots.
Après 18 jours les plants sont observés et des clichés sont pris. After 18 days the plants are observed and clichés are taken.
Tous les échantillons, excepté le béta sitostérol seul (échantillon A), présentent un effet positif sur la tolérance des végétaux au stress. All samples except beta sitosterol alone (sample A) have a positive effect on plant tolerance to stress.
L'efficacité optimale, c'est-à-dire qui permet une meilleure résistance au stress abio tique («flooding») est obtenue avec The optimal efficiency, that is to say that allows a better resistance to abio stress ("flooding") is obtained with
Les résultats montrent que l'échantillon S produit l'effet optimal de résistance de la plante au stress abiotique (échantillon S composé de 80% de sucrose stéarate et 20%> de béta- sitostérol). The results show that sample S produces the optimal effect of resistance of the plant to abiotic stress (sample S composed of 80% sucrose stearate and 20% beta-sitosterol).
On observe un port dressé dans plants traité alors que les plants contrôle ont un port tombant. De plus la coloration des feuilles des plants traités est plus foncée. An upright habit is observed in treated plants while control plants have a falling habit. In addition, the coloring of the leaves of the treated plants is darker.
Les résultats sont présentés dans la figure 1. The results are shown in Figure 1.
L'application de l'invention par arrosage permet une meilleure tolérance au stress «flooding». The application of the invention by watering allows a better tolerance to stress "flooding".
Exemple 2 : Evaluation de l'effet d'un tensioactif non anionique et d'un stérol selon l'invention sur la synthèse d'acide salicylique du persil EXAMPLE 2 Evaluation of the Effect of a Nonionic Surfactant and a Sterol According to the Invention on the Synthesis of Salicylic Acid in Parsley
L'acide salycilique est un composé phénolique qui est impliqué à la fois dans la mise en place d'une résistance locale et d'une résistance générale (SAR) chez les plantes. Salicylic acid is a phenolic compound that is involved in both the establishment of local resistance and general resistance (SAR) in plants.
Des plants de persil en pot sont cultivés selon les conditions de l'exemple 1. Potted parsley plants are grown according to the conditions of Example 1.
L'application des solutions se fait par un arrosage au pied du végétal (40 ml) et une pulvérisation sur les feuilles. Les solutions testées sont : The application of the solutions is done by a watering at the foot of the plant (40 ml) and a spray on the leaves. The tested solutions are:
- Lot Contrôle : eau ; - Lot Control: water;
- lot traité A : solution composée de 97% d'eau et de 3% de l'échantillon A, soit 0% de sucrose stéarate et 100% de béta-sitostérol ; - batch A: solution composed of 97% water and 3% of sample A, ie 0% sucrose stearate and 100% beta-sitosterol;
- lot traité B : solution composée de 97% d'eau et de 3% de l'échantillon Y, 100% de sucrose stéarate et 0%> de béta-sitostérol ; treated batch B: solution composed of 97% of water and 3% of sample Y, 100% of sucrose stearate and 0% of beta-sitosterol;
- lot traité C : solution composée de 97% d'eau et de 3% de l'échantillon S, soit 80% de sucrose stéarate et 20% de béta-sitostérol. treated batch C: solution composed of 97% of water and 3% of the sample S, ie 80% of sucrose stearate and 20% of beta-sitosterol.
Chaque lot est constitué de quatre pots. Each batch consists of four pots.
Les plantes récoltées après l'application du traitement ont été congelées et broyées avec de l'azote liquide pour réaliser une analyse quantitative de la teneur en acide salycilique contenue dans les plantes. Les résultats sont présentés dans la figure 2. Plants harvested after the application of the treatment were frozen and ground with liquid nitrogen to perform a quantitative analysis of the salicylic acid content in the plants. The results are shown in Figure 2.
On observe, de manière inattendue, que la combinaison de sucrose stéarate et de béta- sitostérol (échantillon S) induit une stimulation de la synthèse de l'acide salicylique supérieure à l'addition des effets de chaque composé pris individuellement (échantillon A : béta-sitostérol + échantillon Y : sucrose stéarate), en réponse à un stress biotique. Ces résultats démontrent donc une synergie d'action de ces deux molécules. It is unexpectedly observed that the combination of sucrose stearate and beta-sitosterol (sample S) induces a stimulation of salicylic acid synthesis which is greater than the addition of the effects of each individual compound (sample A: beta -sitosterol + sample Y: sucrose stearate), in response to biotic stress. These results therefore demonstrate a synergistic action of these two molecules.
Exemple 3 : Evaluation de l'effet d'un tensioactif non anionique et d'un stérol selon l'invention sur la germination de graines d'Arabidopsis thaliana Example 3 Evaluation of the Effect of a Nonionic Surfactant and a Sterol According to the Invention on the Germination of Arabidopsis thaliana Seeds
Des graines dArabidopsis thaliana sont placées dans une enceinte climatique sous une photopériode de 16h de jour /8h de nuit, à 22°C. Les graines ont été imbibées avec de l'eau (contrôle) ou avec la composition selon l'invention à des concentrations de 1%, 3% et 10%) dans l'eau. Les graines ont été préalablement stratifiées (c'est-à dire exposées au froid) ou non stratifiées. Arabidopsis thaliana seeds are placed in a climatic chamber under a photoperiod of 16h day / 8h night at 22 ° C. The seeds were imbibed with water (control) or with the composition according to the invention at concentrations of 1%, 3% and 10%) in water. The seeds were previously stratified (that is, exposed to cold) or unstratified.
Différentes étapes de la germination sont observées en fonction du temps : la rupture des téguments (Figures 3 et 4), l'émergence de la radicule (Figure 5 et 6) et l'ouverture des cotylédons (Figures 7 et 8). Different stages of germination are observed as a function of time: the rupture of the integuments (Figures 3 and 4), the emergence of the radicle (Figure 5 and 6) and the opening of the cotyledons (Figures 7 and 8).
Les résultats montrent que l'imbibition des graines avec le mélange de sucrose stéarate et de béta-sitostérol, permet d'accélérer la germination des graines dArabidospis thaliana. Ces résultats sont illustrés par la figure 9 qui représente la germination de ces graines 120 heures après l'imbibition. Exemple 4 : Evaluation de l'effet d'un tensioactif non anionique et d'un stérol selon l'invention sur la croissance racinaire d'Arabidopsis thaliana The results show that the imbibition of the seeds with the mixture of sucrose stearate and beta-sitosterol makes it possible to accelerate the germination of Arabidospis thaliana seeds. These results are illustrated in Figure 9 which shows the germination of these seeds 120 hours after the imbibition. EXAMPLE 4 Evaluation of the Effect of a Nonionic Surfactant and a Sterol According to the Invention on the Root Growth of Arabidopsis thaliana
Des plants & Arabidopsis thaliana sont cultivés sur milieu gélosé dans une enceinte climatique sous les conditions suivantes: 23°C et une photopériode de 16h de jour /8h de nuit. Arabidopsis thaliana plants are cultivated on agar medium in a climatic chamber under the following conditions: 23 ° C and a photoperiod of 16h day / 8h night.
Un milieu gélosé standard est utilisé comme contrôle. Standard agar medium is used as a control.
Des milieux gélosés contenant 10~5% et 10~3% du mélange sucrose stéarate et béta- sitostérol selon l'échantillon S sont mis en œuvre pour évaluer l'effet de l'invention sur le développement du système racinaire. La longueur de la racine principale et des racines secondaires est mesurée entre le 2ème et le 21ème jour post germination. Agar media containing 10 ~ 5 % and 10 ~ 3 % of the sucrose stearate and beta-sitosterol mixture according to the sample S are used to evaluate the effect of the invention on the development of the root system. The length of the main root and secondary roots is measured between the 2nd and 21st day post germination.
La quantité de racines secondaires est évaluée visuellement. Les résultats sont représentés par les figures 10 et 11. The amount of secondary roots is evaluated visually. The results are shown in Figures 10 and 11.
La figure 10 montre que la racine principale des plantes cultivées en présence de 10"5% et 10"3% du mélange selon l'invention est plus longue que pour les plantes cultivées en milieu gélosé contrôle. Figure 10 shows that the main roots of plants grown in the presence of 10 "5% and 10" 3% of the mixture according to the invention is longer than for plants grown in agar medium control.
La figure 11 montre que les plantes cultivées en milieux gélosés comprenant 10"5% et 10" 3% du mélange selon l'invention ont plus de racines secondaires qui sont également plus longue. II résulte de cette démonstration que le mélange sucrose stéarate et béta-sitostérol permet une croissance du système racinaire principal et secondaire. En conséquence, la plante est mieux ancrée dans le sol et la pénétration des nutriments dans la plante est plus efficace en raison duréseau racinaire secondaire plus développé. Figure 11 shows that plants grown in agar media containing 10 "5% and 10" 3% of the mixture according to the invention have more lateral roots that are also longer. It follows from this demonstration that the mixture sucrose stearate and beta-sitosterol allows growth of the main and secondary root system. As a result, the plant is better anchored in the soil and the penetration of nutrients into the plant is more effective due to more extensive secondary root system.
Exemple 5 : Evaluation de l'effet d'un tensioactif non anionique et d'un stérol selon l'invention sur le temps de floraison tfArabidopsis thaliana EXAMPLE 5 Evaluation of the Effect of a Nonionic Surfactant and a Sterol According to the Invention on the Flowering Time tf Arabidopsis thaliana
Des plants àArabidopsis thaliana en pot sont cultivés selon les conditions de l'exemple 1. Plants Arabidopsis thaliana potted are grown according to the conditions of Example 1.
Des solutions comprenant 1, 3 et 10% du mélange sucrose stéarate et béta-sitostérol selon l'échantillon S ou une solution contrôle (eau ; H20) sont pulvérisés une fois (1%, 3% et 10%) ou deux fois à deux jours d'intervalle (1% 2X, 3% 2X et 10%> 2X), sur des plantes âgées de 3 semaines. Solutions comprising 1, 3 and 10% of the sucrose stearate and beta-sitosterol mixture according to sample S or a control solution (water, H 2 O) are sprayed once (1%, 3% and 10%) or twice at two times. days apart (1% 2X, 3% 2X and 10%> 2X), on plants 3 weeks old.
Le nombre de plantes fleuries par modalités a été mesuré en fonction du temps. The number of flowering plants by modalities was measured as a function of time.
Les résultats sont représentés par la figure 12. The results are shown in Figure 12.
Les résultats montrent que la solution contenant 10% du mélange selon l'invention et pulvérisé une fois, de même que la solution comprenant 1% du mélange et pulvérisé deux fois, ont la même efficacité que l'eau sur la floraison des plantes. The results show that the solution containing 10% of the mixture according to the invention and sprayed once, as well as the solution comprising 1% of the mixture and sprayed twice, have the same efficiency as water on the flowering of the plants.
En revanche, une seule pulvérisation sur les plantes des solutions comprenant 1% ou 5% du mélange augmente le nombre de plantes fleuries par rapport au contrôle. On the other hand, a single spray on the plants of the solutions comprising 1% or 5% of the mixture increases the number of flowering plants compared to the control.
En conséquence, le mélange sucrose stéarate et béta-sitostérol selon l'invention accélère la floraison à' Arabidopsis thaliana. As a result, the sucrose stearate and beta-sitosterol mixture according to the invention accelerates flowering to Arabidopsis thaliana.
Exemple 6 : Evaluation de l'effet d'un tensioactif non anionique et d'un stérol selon l'invention sur la déperdition d'eau stomatique tfArabidopsis thaliana EXAMPLE 6 Evaluation of the Effect of a Nonionic Surfactant and a Sterol According to the Invention on the Loss of Stomatic Water tf Arabidopsis thaliana
Des plants d'Arabidopsis thaliana en pot sont cultivés selon les conditions de l'exemple 1. Potted Arabidopsis thaliana plants are cultivated according to the conditions of Example 1.
Les plantes, âgées de 4 semaines, sont traitées par une pulvérisation foliaire, 4 heures avant que les mesures soient effectuées, avec de l'eau (Contrôle) ou une solution comprenant 3% du mélange sucrose stéarate et béta-sitostérol selon l'échantillon S. Plants, 4 weeks old, are treated with a foliar spray, 4 hours before the measurements are made, with water (Control) or a solution comprising 3% of the mixture sucrose stearate and beta-sitosterol according to the sample S.
La quantité d'eau perdue par les stomates au cours du temps est mesurée après détachement de la rosette par rapport à la masse d'eau totale à t=0, selon la formule suivante : The amount of water lost by the stomata over time is measured after detachment of the rosette relative to the total body of water at t = 0, according to the following formula:
masse fraîche t = 0— masse t = T  cool mass t = 0- mass t = T
Perte (%) = '—— lOO  Loss (%) = '- 100
masse fraîche t = 0— masse sèche  fresh mass t = 0- dry mass
Les résultats sont représentés par la figure 13.  The results are shown in Figure 13.
Les données montrent que l'application par pulvérisation de sucrose stéarate et béta- sitostérol selon l'invention permet de diminuer la déperdition d'eau par les stomates de la plante de façon efficace et durable dans le temps. Exemple 7 : Evaluation de l'effet mouillant d'un tensioactif non anionique et d'un stérol selon l'invention sur Arabidopsis thaliana The data show that the spray application of sucrose stearate and beta-sitosterol according to the invention makes it possible to reduce the water loss by the stomata of the plant in an efficient and durable manner over time. Example 7 Evaluation of the wetting effect of a non-anionic surfactant and a sterol according to the invention on Arabidopsis thaliana
Des plants àArabidopsis thaliana en pot sont cultivés selon les conditions de l'exemple 1. Plants Arabidopsis thaliana potted are grown according to the conditions of Example 1.
Les feuilles àArabidopsis thaliana sont traitées par une pulvérisation foliaire, avec de l'eau (Contrôle) ou une solution comprenant 3% du mélange sucrose stéarate et béta- sitostérol selon l'échantillon S. The leaves with Arabidopsis thaliana are treated with a foliar spray, with water (Control) or a solution comprising 3% of the sucrose stearate and beta-sitosterol mixture according to the sample S.
Le nombre de gouttelettes présentes sur la surface de la feuille de la plante est quantifié. The number of droplets present on the leaf surface of the plant is quantified.
Les résultats montrent une diminution du nombre de gouttelettes sur la feuille àArabidopsis thaliana après application du mélange selon l'invention. En d'autres termes, la composition selon l'invention permet de réduire la tension de surface de la solution pulvérisée sur les feuilles et donc permet un meilleur étalement des gouttelettes et une meilleure adhésion de la solution sur la feuille. The results show a decrease in the number of droplets on the Arabidopsis thaliana leaf after application of the mixture according to the invention. In other words, the composition according to the invention makes it possible to reduce the surface tension of the sprayed solution on the sheets and thus allows better spreading of the droplets and better adhesion of the solution to the sheet.
En outre, les résultats montrent qu'il n'y a pas d'accumulation de la solution comprenant le mélange sucrose stéarate et béta-sitostérol selon l'invention au niveau des nervures et des bourgeons axillaires de la plante. In addition, the results show that there is no accumulation of the solution comprising the mixture sucrose stearate and beta-sitosterol according to the invention in the ribs and axillary buds of the plant.
En conséquence, le mélange du sucrose stéarate et du béta-sitostérol selon l'invention permet notamment de traiter les graminées connues pour être peu mouillables. Consequently, the mixture of sucrose stearate and beta-sitosterol according to the invention makes it possible in particular to treat grasses known to be poorly wettable.
Un autre intérêt est l'utilisation de ce mélange en tant qu'adjuvant pour les produits qui ont une action de contact. Another advantage is the use of this mixture as an adjuvant for products that have a contact action.
Par ailleurs, l'utilisation du mélange selon l'invention permet d'augmenter la couverture de la plante et d'utiliser moins de produit à l'hectare. Moreover, the use of the mixture according to the invention makes it possible to increase the cover of the plant and to use less product per hectare.

Claims

REVENDICATIONS
1/ Utilisation d'au moins un tensioactif non ionique dérivé de polyols et un stérol comme biostimulant d'un végétal. 1 / Use of at least one nonionic surfactant derived from polyols and a sterol as a biostimulant of a plant.
21 Utilisation selon la revendication 1 pour favoriser la germination. 3/ Utilisation selon la revendication 1 pour favoriser l'allongement des racines principales et la multiplication et l'allongement des racines secondaires. Use according to claim 1 for promoting germination. 3 / Use according to claim 1 to promote the elongation of the main roots and the multiplication and elongation of secondary roots.
4/ Utilisation selon la revendication 1 pour favoriser la floraison. 4 / Use according to claim 1 to promote flowering.
5/ Utilisation selon la revendication 1 , pour lutter contre le stress abiotique, éventuellement la déperdition d'eau stomatique. 6/ Utilisation d'au moins un tensioactif non ionique dérivé de polyols et un stérol pour stimuler des mécanismes de défense naturelle contre un stress biotique. 5 / Use according to claim 1, for combating abiotic stress, possibly the loss of stomatal water. 6 / Using at least one nonionic surfactant derived from polyols and a sterol to stimulate natural defense mechanisms against biotic stress.
7/ Utilisation selon la revendication 6, caractérisée en ce que le mécanisme de défense naturelle est le système de résistance systémique acquise. 7 / Use according to claim 6, characterized in that the natural defense mechanism is the acquired systemic resistance system.
8/ Utilisation d'au moins un tensioactif non ionique dérivé de polyols et un stérol comme adjuvant d'une solution contenant un produit actif vis-à-vis de la plante. 8 / Use of at least one nonionic surfactant derived from polyols and a sterol as an adjuvant of a solution containing an active product with respect to the plant.
91 Utilisation selon la revendication 8, caractérisée en ce que le produit actif est choisi dans le groupe comprenant les nutriments, un ou plusieurs engrais, un ou plusieurs régulateur de croissance et/ou avec un produit de biocontrôle, lui-même choisi dans le groupe comprenant les fongicides, les fongistatiques, les bactéricides, les bactériostatiques, les insecticides, les acaricides, les parasiticides, les nématicides, les taupicides ou les répulsifs pour oiseaux ou gibiers, une ou plusieurs substances qui visent à détruire les végétaux indésirables ou en ralentir leur croissance choisie notamment parmi des herbicides ou des antidicotylédones. 91 Use according to claim 8, characterized in that the active product is selected from the group comprising nutrients, one or more fertilizers, one or more growth regulator and / or with a biocontrol product, itself chosen from the group including fungicides, fungicides, bactericides, bacteriostats, insecticides, acaricides, parasiticides, nematicides, taupicides or repellents for birds or game, one or more substances intended to destroy unwanted plants or to slow down their growth. growth chosen especially from herbicides or antidicotyledons.
10/ Utilisation selon l'une des revendications précédentes, après l'émergence ou avant l'émergence, sur la graine, la plantule (stade juvénile antérieur à la floraison), la plante en cours de floraison (avant, pendant ou après pollinisation), la plante après fécondation, la plante en cours de fructification, le fruit, les fleurs, les feuilles, les tiges, les racines ou dans le sol, avant ou après semis. 10 / Use according to one of the preceding claims, after emergence or before emergence, on the seed, the seedling (juvenile stage prior to flowering), the plant during flowering (before, during or after pollination) , the plant after fertilization, the plant being fruited, the fruit, flowers, leaves, stems, roots or in the soil, before or after sowing.
11/ Utilisation selon l'une des revendications précédentes, caractérisée en ce que le végétal est choisi dans le groupe comprenant les Dicotylédones et les Monocotylédones, avantageusement dans le groupe comprenant les céréales, les plantes à racines et tubercules, les saccharifères, les légumineuses, les végétaux à fruits à coques, les plantes oléifères ou oléagineuses, les plantes de culture légumière, les fruitiers, les plantes aromatiques et les épices, les plantes de cultures florales, ou encore les plantes de culture industrielle destinée à la production d'une matière première en vue de sa transformation. 11 / The use according to one of the preceding claims, characterized in that the plant is selected from the group comprising Dicotyledonous and Monocotyledonous, advantageously in the group comprising cereals, root and tuber crops, saccharifères, legumes, nuts, oil plants, or oleaginous plants, vegetable crops, fruit trees, aromatic plants and spices, flowering plants, or industrial crops intended for the production of a raw material for processing.
12/ Utilisation selon l'une des revendications précédentes, caractérisée en ce que le tensioactif non ionique dérivé de polyols est choisi dans le groupe comprenant les esters de sucre et d'acides gras, les alkyl-mono- et alkyl-poly-glucosides, les esters d'alkyl- mono- et alkyl-poly-glucosides et d'acides gras et les N-alkylglucamides, en particulier les N-méthylglucamides. 12 / Use according to one of the preceding claims, characterized in that the nonionic surfactant derived from polyols is selected from the group consisting of sugar esters of fatty acids, alkyl-mono- and alkyl-poly-glucosides, esters of alkyl-mono- and alkyl-polyglycosides and of fatty acids and N-alkylglucamides, in particular N-methylglucamides.
13/ Utilisation selon l'une des revendications précédentes, caractérisée en ce que le tensioactif non ionique dérivé de polyols est choisi dans le groupe comprenant les esters de saccharose, les esters de sorbitan et les esters de glucose. 13 / Use according to one of the preceding claims, characterized in that the nonionic surfactant derived from polyols is selected from the group comprising sucrose esters, sorbitan esters and glucose esters.
14/ Utilisation selon l'une des revendications précédentes, caractérisée en ce que le tensioactif non ionique dérivé de polyols est éthoxylé ou non éthoxylé. 14 / Use according to one of the preceding claims, characterized in that the nonionic surfactant derived from polyols is ethoxylated or non-ethoxylated.
15/ Utilisation selon l'une des revendications précédentes, caractérisée en ce que le tensioactif non ionique dérivé de polyols est le stéarate du saccharose. 15 / Use according to one of the preceding claims, characterized in that the nonionic surfactant derived from polyols is sucrose stearate.
16/ Utilisation selon l'une des revendications précédentes, caractérisée en ce que le stérol est choisi parmi le cholestérol, les stérols végétaux comme le campestérol, le béta- sitostérol, le stigmastérol, le brassicastérol, le campestanol, le sitostanol, les stérols animaux comme le lanostérol ou les stérols de levures ou champignons comme l'ergostérol. 16 / Use according to one of the preceding claims, characterized in that the sterol is selected from cholesterol, plant sterols such as campesterol, beta-sitosterol, stigmasterol, brassicasterol, campestanol, sitostanol, animal sterols such as lanosterol or yeast sterols or fungi such as ergosterol.
17/ Utilisation selon l'une des revendications précédentes, caractérisée en ce que le stérol est le béta-sitostérol. 17 / The use according to one of the preceding claims, characterized in that the sterol is beta-sitosterol.
18/ Utilisation selon l'une des revendications précédentes, caractérisée en ce que le tensioactif non ionique dérivé de polyols et le stérol sont en solution, avantageusement sous la forme d'une solution aqueuse, comprenant 0.01% à 80%, de préférence 0.05%> à 30%>, de façon encore plus préférentielle 0.75%> à 3 % en poids de la solution de tensioactif non ionique dérivé de polyols et de stérol. 18 / Use according to one of the preceding claims, characterized in that the nonionic surfactant derived from polyols and the sterol are in solution, preferably in the form of an aqueous solution, comprising 0.01% to 80%, preferably 0.05% > 30%>, still more preferably 0.75%> to 3% by weight of the solution of nonionic surfactant derived from polyols and sterol.
19/ Utilisation selon l'une des revendications précédentes, caractérisée en ce que le au moins un tensioactif non ionique dérivé de polyols est le stéarate du saccharose et le stérol est le béta-sitostérol. 19 / The use according to one of the preceding claims, characterized in that the at least one nonionic surfactant derived from polyols is sucrose stearate and the sterol is beta-sitosterol.
20/ Utilisation selon l'une des revendications précédentes, caractérisée en ce que le tensioactif non ionique dérivé de polyols et le stérol sont appliqués sur le végétal par pulvérisation, arrosage de la plante, par addition à un milieu de culture en hydroponie, par immersion de la graine et/ou par enrobage de la graine. 20 / Use according to one of the preceding claims, characterized in that the nonionic surfactant derived from polyols and the sterol are applied to the plant by spraying, watering the plant by addition to a hydroponic culture medium, by immersion of the seed and / or by coating the seed.
21/ Composition comprenant le stéarate de saccharose et le béta-sistostérol. 21 / Composition comprising sucrose stearate and beta-sistosterol.
22/ Composition la revendication 21, caractérisée en ce que le béta-sistostérol est compris entre 1 et 99 % en poids de la composition et le stéarate de saccharose est compris entre 99 et 1% en poids de la composition, de préférence le béta-sistostérol est compris entre 40 et 1 % et le stéarate de saccharose est compris entre 60 et 99 % en poids de la composition. 22 / Composition claim 21, characterized in that the beta-sistosterol is between 1 and 99% by weight of the composition and the sucrose stearate is between 99 and 1% by weight of the composition, preferably the beta-sistosterol is sistosterol is between 40 and 1% and the sucrose stearate is between 60 and 99% by weight of the composition.
EP18755875.4A 2017-08-07 2018-07-26 Composition made from polyol(s) and sterol(s) for use in the agricultural field Pending EP3664612A1 (en)

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