KR102360226B1 - Composition containing rare sugars as an active ingredient for inhibiting growth of Ambrosia artemisiifolia and Ambrosia trifida, and growth regulator and herbicide using the compositoin - Google Patents

Abstract

The present invention relates to a composition which can inhibit the growth of Ambrosia artemisiifolia L. and Ambrosia trifida L. that are forest invasive exotic plants using a rare sugar harmless to the human body, and to a growth regulator and an herbicide using the composition. In the above, the inhibition functions of growth comprise effects of seed germination inhibition, early growth inhibition, shoot growth inhibition, seed fructification amount inhibition, and apoptosis.

Description

Composition containing rare sugars as an active ingredient for inhibiting the growth of ragweed and maple leaf ragweed, and a growth regulator and herbicide using the composition {Composition containing rare sugars as an active ingredient for inhibiting growth of Ambrosia artemisiifolia and Ambrosia trifida, and growth regulator and herbicide using the composite}

The present invention relates to a composition capable of inhibiting the growth of ragweed and maple leaf ragweed, which are foreign plants invading forests, using rare sugars harmless to the human body, and a growth regulator and herbicide using the composition. In the above, the inhibition of growth includes seed germination inhibition, early growth inhibition, above-ground part growth inhibition, seed deletion amount inhibition, and apoptosis functions .

The present invention relates to a composition for inhibiting the growth of ragweed and maple leaf ragweed, which are exotic forest invasion species, and outlines of ragweed and maple leaf ragweed are as follows.

Ragweed ( Ambrosia artemisiifolia L.) is an annual and germinates around April, grows upright, and has soft hairs all over the row. The leaves are opposite at the bottom of the stem and opposite at the top. It has 2 upper right bivalve leaves, the front side is dark green, and the back side is grayish with soft hairs. Flowers bloom in single male and female clusters in August-September in the shape of an ear, and the male double flowers are in a raceme, and several bloom downwards, producing a large amount of yellow pollen. A female flower head consists of one female flower, and several are on the lower part of the inflorescence. The fruit is a skinny fruit with a hard shell and is in the shape of a queen's crown.

Ragweed causes allergic pollen disease due to pollen produced in large quantities from late summer to early autumn. Therefore, it has been designated as a harmful alien species to be removed.

Maple leaf ragweed ( Ambrosia trifida L.) is a species imported from the United States. Leaves are opposite, deeply split into 3-5 like maple leaves, each 20-30cm in length and width, the lower part suddenly flows into petioles, there are setae on both sides, and the lobes are oval-lanceolate, with sawtooths on the edges, and flat ends. However, it becomes sharp and the petiole is about 6cm long, has grooves on the surface, has white hairs along with the edges, and the bottom part is widened to cover the main stem.

Flowers bloom in July-September, hang in raceme, and one or several female flowers are clustered into heads and hang at the bottom, with many male flowers blooming from above. The bract of the male flower is dish-shaped, has a pedicel, is regular, has 3 ridges on one side, and the bract of the female flower is a pointed ellipsoid, containing 1 flower, and the split style comes out.

The fruit is egg-shaped, 6-12mm long, with 2-4mm long projections at the tip, and 4-10 fine projections on the ridgeline.

Maple leaf ragweed pollen not only causes allergic rhinitis, but also various respiratory diseases and dermatitis. It is an ecosystem disturbance wild plant designated by the government.

Plant growth regulators, herbicides, etc. are used as substances that help to grow plants.

Plant growth regulators are agents that promote plant growth or, conversely, inhibit or artificially induce abnormal growth, and are used to produce economical agricultural products. Growth regulators known until recently include growth promoters such as atonic, gibberellin, and tomato tone, rooting promoters such as Luton, color promoters such as etepon, fall inhibitors such as 2,4,5-TP, maleic acid hydrazide (Mach), etc. There are growth inhibitory agents.

Herbicides are chemical agents used to remove weeds, and depending on the scope of application, they are divided into non-selective herbicides that kill all plants in the sprayed area and selective herbicides that can be used to control weeds during crop cultivation by killing only specific plant species. According to the mechanism of action, herbicides are divided into contact herbicides, which kill only the part that the drug directly comes into contact with, and penetrative herbicides, which are absorbed into the plant and migrate to the leaves, stems, and roots, inhibiting plant metabolism and growth, leading to death. There are soil herbicides and foliar herbicides depending on the location of herbicide absorption. A pre-germination treatment agent that kills weed seeds by spraying it on the soil before weeds germinate, a post-emergence treatment agent that targets already growing weeds, and herbicides are also classified according to the time of use.

Commonly used herbicides include ① aliphatic powders: 2,4-D, 2,4,5-T, 2,4-DP, MCPA, MCPP, MCPB, glyphosate, ② phenols: PCP, ③ amides: Propanil, butachlor (Macet), alachlor (Lasso), ④ carbamate: chlorpropam, fenmedipham (betanal), thiovencarb (Satan), ⑤ urea: metabenzthiazuron (Tri) bennyl), ⑥ triazine: atrazine, simazine, ⑦ bipyridylium: paraquat (graoxone), ⑧ uracil: bromacil (HibaX), bentazone (vasagran), ⑨ other organic herbicides: text Liposate (approximately), and the like. Such herbicides are disclosed in 'Patent Document 1' and the like.

However, plant growth regulators and herbicides are artificially synthesized chemicals or plant hormones. In particular, substances that inhibit plant growth (mainly weeds) are chemical substances, so if they are used for vegetables or fruits that people mainly eat, they may accumulate in the human body or cause serious diseases.

The present applicant has received a patent for a composition for inhibiting germination and plant growth containing rare sugar harmless to the human body as an active ingredient, and a plant growth regulator and herbicide using the same with registration number 10-2096485. Referring to claim 1, it can be seen that the functions for Gujeolcho and Western Geumhoncho have been recognized.

KR 10-2019-0123293 A1 (2019. 10. 31.) KR 10-2096485 B1 (2020.03.27.)

An object of the present invention is to provide a growth inhibitory composition using a substance that is not harmful to the human body, a growth regulator and a herbicide using the composition.

The problem to be solved by the present invention is to provide a growth inhibitory composition using substances that are not harmful to the human body as an active ingredient, a growth regulator using the composition, and a manufacturer for ragweed and maple leaf ragweed, which are foreign plants invading forests will be.

Another method for solving the present invention is to provide a composition for inhibiting the growth of ragweed ( Ambrosia artemisiifolia L.) and maple leaf ragweed ( Ambrosia trifida L.) containing allulose (D-allulose) as an active ingredient.

The allulose is characterized in that it is used in a concentration of 5% or more.

The allulose is

It is characterized in that it can be replaced with one or more of allulose and allulose (D-allose) mixed materials through an enzymatic reaction of allulose or allulose.

The growth inhibition is characterized in that it includes seed germination inhibition, above-ground part growth inhibition, seed deletion amount inhibition, and apoptosis functions.

The problem solving means according to the present invention is to provide a ragweed growth regulator comprising the composition. In addition, it is to provide a ragweed herbicide comprising the composition. In addition, it is to provide a growth regulator of maple leaf ragweed comprising the composition. In addition, it is to provide a maple leaf ragweed herbicide comprising the composition.

The composition according to the present invention is harmless to the human body because it can inhibit the growth of plants by using rare sugars. In particular, by using allulose and allose as rare sugars, it has a remarkable effect of inhibiting the growth of ragweed and maple leaf ragweed, which are exotic forest invasion plants. In addition, it includes remarkable effects up to seed germination inhibition, early growth inhibition, above-ground part growth inhibition, seed loss amount inhibition, and death function.

1A is a photograph showing the results of suppression of germination of ragweed seeds added to each concentration of allulose.
Figure 1b is a graph showing the change in the germination rate of ragweed seeds in the medium to which allulose is added for each concentration.
Figure 2a is a photograph of the result of suppression of germination of ragweed seeds to which allose was added by concentration.
Figure 2b is a graph showing the change in the germination rate of ragweed seeds in the medium to which allose is added for each concentration.
Figure 3a is a photograph of the results of suppression of germination of ragweed seeds added to each concentration of the mixed material.
Figure 3b is a graph showing the change in the germination rate of ragweed seeds in the medium to which the mixed material is added by concentration.
4 is a graph summarizing the test results of seed germination inhibition of the experimental group for ragweed.
Figure 5a is a photograph of the result of germination inhibition of maple leaf ragweed seeds to which allulose was added by concentration.
Figure 5b is a graph showing the change in the germination rate of Maple Leaf ragweed seeds in the medium to which allulose is added by concentration.
Figure 6a is a photograph of the result of suppression of germination of maple leaf ragweed seeds added to each concentration of the mixed material.
Figure 6b is a graph showing the change in the germination rate of maple leaf ragweed seeds in the medium to which the mixed material is added by concentration.
7 is a graph summarizing the test results of seed germination inhibition of the experimental group for maple leaf ragweed.
8 is a photograph of growth change after 1 day and 10 days after treatment with 5% concentration of allulose in ragweed.
9 is a photograph showing the growth change after 1 day and 10 days after treatment with 1% concentration of allulose in ragweed.
10 is a photograph of growth change 10 days after treatment with 5% concentration of glucose in ragweed.
11 is a graph showing the change in the length of the above-ground part of the maple leaf ragweed superior group according to the sugar treatment for each concentration.
12 and 13 are photographs of growth change after 28 days of the Maple Leaf ragweed superior group.
14 is a graph comparing the amount of seed loss in the superior group of maple leaf ragweed according to the allulose treatment.
15 is a graph showing the change in the length of the above-ground part of the maple leaf ragweed inner group according to the sugar treatment for each concentration.
16 and 17 are pictures of the growth change after 28 days and 80 days of the Maple Leaf ragweed Inferior group.

The terms or words used in the present specification and claims conform to the technical spirit of the present invention based on the "principle that the inventor can appropriately define the concept of a term in order to best describe his invention" It should be interpreted as the meaning and concept that

Therefore, the embodiments described in this specification and the configurations shown in the drawings are only the most preferred embodiments of the present invention, and do not represent all the technical spirit of the present invention, so various equivalents that can replace them at the time of the present application It should be understood that there may be water and variations.

Experimental method 1. Experiment for suppressing seed germination of ragweed and maple leaf ragweed

For the in-flight germination inhibition test, ragweed seeds and maple leaf ragweed seeds were sterilized with 70% EtOH for about 2 minutes, surface disinfected with 2.5% sodium hypochlorite for about 1 hour, and washed 5 times with sterile distilled water to prepare.

For the seed germination inhibition screening , 3 types of allulose, D-allose, and mixed material (D-allulose:D-allose = 7:3 concentration ratio produced through the enzymatic reaction of D-allulose) Natural saccharides were used and treated by adding them to 0.8% Agar at concentrations of 0.01%, 0.1%, 1%, and 2%, respectively .

After the sterilized seeds were dented, vernal flowers were treated at 4°C for 2 days, followed by observation at 25°C and 12h/12h conditions for 14 days.

Experiment Result 1-1. Inhibition of seed germination of ragweed

[Allulose]

1 is a photograph showing the results of suppression of germination of ragweed seeds added by concentration of allulose.

Figure 2 is a graph showing the change in the germination rate of ragweed seeds in the medium to which allulose is added for each concentration.

As the concentration of D-allulose treatment increased, the germination rate decreased ( 18% inhibition at 0.1% ), and germination was inhibited by up to 40% (based on 2% treatment group) compared to the control group, untreated group. In addition, as the concentration of D-allulose increased, root development and leaf opening of germinated individuals were not performed properly, and most of them did not grow normally.

[Allos]

Figure 2a is a photograph of the result of suppression of germination of ragweed seeds to which allose was added by concentration.

Figure 2b is a graph showing the change in the germination rate of ragweed seeds in the medium to which allose is added for each concentration.

There was no significant difference in the germination rate between the control group and the treatment group when treated by D-allose concentration, and it was observed that the sugar treatment group showed better root development than the control group, such as better growth.

[Mixed Material]

Figure 3a is a photograph of the results of suppression of germination of ragweed seeds added to each concentration of the mixed material. Figure 3b is a graph showing the change in the germination rate of ragweed seeds in the medium to which the mixed material is added by concentration.

When the mixed material mixed with D-allulose and D-allose in a ratio of 7:3 through D-allulose enzyme reaction was treated by concentration, the germination rate was decreased by about 33% compared to the untreated group when treated with 2%, and the sugar concentration was increased Accordingly, it was confirmed that most of them did not grow normally because root development and leaf opening were not performed properly.

[Synthesis]

4 is a graph summarizing the test results of seed germination inhibition of the experimental group for ragweed. There was a difference in the control of ragweed seed germination according to the type of sugar, and it was confirmed that treatment with high concentration (2%) of D-allulose or mixed material inhibited germination most effectively. In addition, it was confirmed that allulose alone showed a significant effect from 0.1%.

Experimental results 1-2. Inhibition of seed germination of maple leaf ragweed

[Allulose]

Figure 5a is a photograph of the result of germination inhibition of maple leaf ragweed seeds to which allulose was added by concentration. Figure 5b is a graph showing the change in the germination rate of Maple Leaf ragweed seeds in the medium to which allulose is added by concentration.

When treated with D-allulose concentration, the germination rate was suppressed by up to 60% (based on 2% treatment group) compared to the control group, untreated group.

[Mixed Material]

Figure 6a is a photograph of the result of suppression of germination of maple leaf ragweed seeds added to each concentration of the mixed material. Figure 6b is a graph showing the change in the germination rate of maple leaf ragweed seeds in the medium to which the mixed material is added by concentration.

When the mixed material mixed with D-allulose and D-allose in a ratio of 7:3 through D-allulose enzyme reaction was treated by concentration, it was inhibited by up to 51% (based on 1% treatment group) compared to the control group, untreated group, and was suppressed by more than 1% During treatment, root development and leaf opening were not performed properly, so most of them did not grow normally.

[Synthesis]

7 is a graph summarizing the test results of seed germination inhibition of the experimental group for maple leaf ragweed.

Both D-allulose and the mixed material (D-allulose 7: D-allose 3) showed effects in inhibiting the germination of maple leaf pigweed seeds. It is considered to be effective for pool control.

Example 1. Composition for inhibiting seed germination of ragweed or maple leaf ragweed containing rare sugar as an active ingredient, and growth regulator and herbicide using the composition

The composition for inhibiting seed germination of ragweed or maple leaf ragweed according to the present invention contains allulose as an active ingredient.

When allulose is used as an active ingredient of the composition for inhibiting ragweed seed germination, it is preferable that allulose in a concentration of 0.1% or more is included. When allulose is used as an active ingredient of the composition for inhibiting seed germination of maple leaf ragweed, it is preferable that allulose in a concentration of 1% or more is included.

The composition for inhibiting seed germination of ragweed or maple leaf ragweed according to the present invention includes allulose and an allose mixed material as an active ingredient.

When an allulose and allose mixed material is used as an active ingredient in the composition for inhibiting seed germination of ragweed or maple leaf ragweed, it is recommended that the concentration ratio of D-allulose: D-allose be 7:3 through the enzymatic reaction of D-allulose. desirable.

When the mixed material is used in the composition for inhibiting ragweed, the concentration of the mixed material is preferably 2% or more. When the mixed material is used in the composition for inhibiting seed germination of maple leaf ragweed, it is preferable that the mixed material be included in a concentration of 1% or more.

By utilizing the seed germination inhibitory function of ragweed and maple leaf ragweed of allulose or the mixed material, a growth regulator or herbicide containing allulose or the mixed material may be implemented. It goes without saying that other ingredients may be included to suit the conditions for manufacturing a growth regulator or herbicide depending on the design conditions.

Experimental method 2. Growth inhibition experiment of ragweed and maple leaf ragweed

To test the growth inhibitory effect, after disinfection, ragweed germinated in the plane was transplanted into a pot, and then cultivated in the open field for about 2 months, and 1% and 5% D-allulose was applied to the individuals with good growth during the middle growth period (when the flower stalks rise). Start), the soil and foliage were treated once, and observations were made 1 day and 10 days later, respectively .

In addition, to determine whether the result of 5% D-allulose treatment was the effect of sugar or dehydration due to high concentration treatment, 5% glucose was treated as a control and observed.

For maple leaf ragweed, a 1x1 (m) square ball was installed in a colony located in the National Baekdudaegan Arboretum in Chunyang-myeon, Bonghwa-gun, Gyeongsangbuk-do, and the growth inhibitory effect was tested by dividing it into Superior and Inferior groups based on the growth level and appearance frequency.

In the case of maple leaf ragweed, D-allulose and mixed material (D-allulose(7):D-allose(3), produced at a concentration ratio of 7:3 through enzymatic reaction of D-allulose) are used in total using two types of natural sugars. In the early stage of growth, the soil was treated at 1%, 5% concentration (allulose), and 10% concentration (mixed) for 80 days, 3 times at intervals of 1 to 2 weeks in the beginning of growth, once in the middle of growth for a total of 4 times, each 4L each observed .

In May, 30 maple leaf ragweed per square were selected and the length of the above-ground part was measured. In order to confirm the change in the length of the above-ground part, it was measured three times per period.

For the growth control test, in order to test whether there is a change in the yield of D-allulose-treated Maple Leaf ragweed, at the end of August, the flowering period of Maple Leaf ragweed, a seed net was placed on up to 30 specimens per treatment area and collected in early November to check the seed amount. did

Experiment Result 2-1. Inhibition of growth of ragweed

8 is a photograph of growth change after 1 day and 10 days after treatment with 5% concentration of allulose in ragweed. (Left after 1 day / Right after 10 days) After 1 day of treatment with 5% D-allulose, the tip of the leaf was observed to dry slowly, and the lower leaf started to dry out and completely died after 10 days.

9 is a photograph showing the growth change after 1 day and 10 days after treatment with 1% concentration of allulose in ragweed. (Left after 1 day / Right after 10 days) After 1 day of treatment with 1% D-allulose, the lower leaves dried a little, but they grew normally without any significant change until the 10th day.

10 is a photograph of growth change 10 days after treatment with 5% concentration of glucose in ragweed. 5% D-allulose was treated with 5% glucose as a control to determine whether the result of 5% D-allulose treatment was the effect of sugar or dehydration caused by high concentration treatment. It was judged that the effect of allulose treatment was not dehydration caused by sugar treatment.

Experiment result 2-2. Inhibition of growth of maple leaf ragweed

11 is a graph showing the change in the length of the above-ground part of the maple leaf ragweed superior group according to the sugar treatment for each concentration. In the untreated group (Control), the length of the above-ground part increased by an average of 5.6 times (17.1 cm → 113 cm) in 49 days, whereas the 1% D-allulose treatment averaged 4.6 times (17.4 cm → 98.3 cm) and 5% D-allulose averaged 1.9. Pears (19.8cm → 57.8cm) and 10% mixed material increased by 3.9 times (18.9cm → 94cm), confirming that 5% D-allulose treatment effectively inhibited growth by about 1/3 compared to the untreated group .

12 and 13 are photographs of growth change after 28 days of the Maple Leaf ragweed superior group. After 28 days of treatment with 5% D-allulose, most of the lower leaves of the plant dried up, but most of the upper leaves grew normally. did

14 is a graph comparing the amount of seed loss in the superior group of maple leaf ragweed according to the allulose treatment. The untreated group of the Superior group produced an average of 40 seeds per individual, but the 1% and 5% D-allulose treatment groups produced an average of 23 seeds, which decreased by about 43% per individual, showing a significant difference.

15 is a graph showing the change in the length of the above-ground part of the maple leaf ragweed inner group according to the sugar treatment for each concentration. In the untreated group (Control), the length of the above-ground part increased by an average of 3.9 times (10.3cm → 50.7cm) in 49 days, whereas the 1% D-allulose treatment averaged 3 times (11.3cm → 46.1cm), and the 5% D-allulose averaged 1.4 Pear (12.1cm → 28.8cm) and 10% mixed material increased 3 times (10.8cm → 44.3cm), indicating that the 5% D-allulose treatment effectively inhibited growth by about 1/3 compared to the untreated group, as in the Superior group. Confirmed.

16 and 17 are pictures of the growth change after 28 days and 80 days of the Maple Leaf ragweed Inferior group. After 28 days of treatment with 5% D-allulose, most of the lower leaves of the plant dried up, and the rest of the leaves turned yellow. On the other hand, 1% D-allulose and 10% mixed material had little effect, and did not die until 80 days after treatment.

D-allulose is effective when treated at a higher concentration than in the in-flight test due to the nature of the field test, which is heavily influenced by the environment, such as sunlight, rain, and wind. It was inhibited (1/3 level), and it is judged that the effect as a herbicide is excellent as the treatment at a lower growth stage kills the plant.

Example 2. Composition for inhibiting growth of ragweed or maple leaf ragweed containing rare sugar as an active ingredient, and growth regulator and herbicide using the composition

The composition for inhibiting the growth of ragweed or maple leaf ragweed according to the present invention contains allulose as an active ingredient.

When allulose is used as an active ingredient of the composition for inhibiting the growth of ragweed, it is preferable that allulose in a concentration of 5% or more is included. When allulose is used as an active ingredient of the composition for inhibiting the growth of maple leaf ragweed, it is preferable that allulose in a concentration of 5% or more is included. In the above, the inhibition of growth includes seed germination inhibition, early growth inhibition, above-ground growth inhibition, seed deletion amount inhibition, and apoptosis functions.

A growth regulator or herbicide may be implemented by utilizing the growth inhibitory function of ragweed and maple leaf ragweed of allulose. It goes without saying that other ingredients may be included to suit the conditions for manufacturing a growth regulator or herbicide depending on the design conditions.

So far, the present invention has been focused on preferred examples and experimental examples. The embodiments, experimental examples, and configurations shown in the drawings described in this specification relate to one most preferred embodiment of the present invention, and do not represent all of the technical spirit of the present invention, so various equivalents that can be substituted for them It should be understood that there may be variations and examples.

Therefore, the present invention is not limited to the presented embodiments, and within the equivalent scope of the technical spirit of the present invention and the technical spirit described in the claims to be described below by those of ordinary skill in the technical field to which the present invention pertains. There may be embodiments in which various modifications and changes are possible.

Claims (8)

As a growth inhibition composition comprising allulose (D-allulose) as an active ingredient,
Included are those with a concentration of 5% or more of allulose,
The growth inhibition, characterized in that it includes the suppression of seed germination, inhibition of growth of above-ground parts, inhibition of the amount of seed loss and the function of death,
A composition for inhibiting the growth of ragweed ( Ambrosia artemisiifolia L. ) and maple leaf ragweed ( Ambrosia trifida L. ).
delete The method according to claim 1,
The allulose is
A composition for inhibiting the growth of ragweed and maple leaf ragweed, characterized in that it can be replaced with one or more of allulose and D-allose mixed materials by an enzymatic reaction of allulose or allulose.
delete A ragweed growth regulator comprising the composition of claim 1 or 3.
A ragweed herbicide comprising the composition of claim 1 or 3.
A growth regulator of maple leaf ragweed comprising the composition of claim 1 or 3.
A maple leaf ragweed herbicide comprising the composition of claim 1 or 3.

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