CN105230480A - Breeding method for novel glyphosate-herbicide-resistant rice germplasm - Google Patents
Breeding method for novel glyphosate-herbicide-resistant rice germplasm Download PDFInfo
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- CN105230480A CN105230480A CN201510510087.1A CN201510510087A CN105230480A CN 105230480 A CN105230480 A CN 105230480A CN 201510510087 A CN201510510087 A CN 201510510087A CN 105230480 A CN105230480 A CN 105230480A
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Abstract
The invention relates to the field of rice germplasm resources and particularly relates to a breeding method for novel glyphosate-herbicide-resistant rice germplasm. The method comprises the steps of carrying out seed preparation, carrying out irradiation mutagenesis, carrying out field planting 1, carrying out resistant plant screening 1, carrying out field planting 2, carrying out chemical mutagenesis, carrying out resistant plant screening 2, carrying out hybridization grouping, carrying out field planting 3, carrying out resistant plant screening 3 and the like. The novel glyphosate-herbicide-resistant rice germplasm obtained by the method has the following beneficial characteristics that the germplasm is non-transgenic; the germplasm has relatively high resistance to glyphosate herbicides; the germplasm is safe in use; and the germplasm is convenient in use.
Description
Technical field
The present invention relates to Rice Germplasm Resources field, particularly relate to a kind of breeding method of glyphosate tolerant weed killer herbicide paddy rice new germ plasm.
Background technology
Along with the fast development of modern agriculture science and technology, the advantage that herbicides for use in paddy saves labor with it obtains spread.Weeds in paddy field mainly contains moleplant seed gramineous, barnyard grass, the difformed galingale herb of sedge family, Scirpus tabernaemontani, the Procumbent Falsepimpernel of Scrophulariaceae, lythraceous mexicana, the ring dish leaf of Commelianaceae, four leaf duckweeds of Marsilaceae, the water pepper of polygonaceae, the alternanthera philoxeroides of Amaranthaceae, the short arrowhead etc. of Alismataceae.The herbicides for use in paddy that current production is applied mostly is the selective herbicides such as 20% Stam F-34 missible oil, 50% Youkebai missible oil, young standing grain guarantor, and they only rise a certain class weeds in rice field and kill or inhibitory action, and cannot play herbicidal effect to another kind of weeds.Glyphosate herbicidal is that a kind of wide spectrum goes out natural disposition, inner-adsorption conduction-type herbicide, and trade name has agriculture to reach, Roundup, Rodeo, Accord etc., and it is absorbed by cauline leaf and conducts to complete stool, makes weeds withered, to annual and perennial weed is all effective.Because it is good and cheap, toxicity is little, meeting water and decompose rapidly, be considered at present pollute one of minimum, safest commercial herbicides, but glyphosate herbicidal plays kill activity too to existing rice varieties.
Applying transgene resistance glyphosate weed killer herbicide kind, though can reach and spray glyphosate and go out and do not damage the object of rice growth except weeds in paddy field, but based on the application procedures of transformed variety commercialization complexity and people to the worry of transgenic rice safety, present stage is difficult to apply in production, and transgenosis resistance glyphosate weed killer herbicide rice varieties is applied also by the restriction of foreign patent in China.
Therefore, formulate the non-transgenic glyphosate tolerant kind matter seed selection glyphosate tolerant weed killer herbicide new rice variety with independent intellectual property right, significant to promotion China Rice Production.
Summary of the invention
Application for Glyphosate resistance gene relies on transgenic technology and the present situation controlled by foreign patent, initiative has the non-transgenic glyphosate tolerant kind matter of independent intellectual property right and the new rice variety of seed selection glyphosate tolerant weed killer herbicide, and reaching saves labor effectively removes the object of weeds in paddy field, increasing both production and income.
The step that the present invention formulates glyphosate tolerant weed killer herbicide paddy rice new germ plasm method is as follows:
(1) seed preparation.Select the conventional Rice govern-house-variety of local spread, as late japonica rice variety Zhejiang round-grained rice 22(New species right in plant CNA20070398.6), seedling is strengthened in light seeding, and this plantation single, individual plant is gathered in the crops.Heading and maturing stage are paid special attention to remove special-shaped strain.
(2) irradiation mutagenesis.The dry seeds that step (1) is gathered in the crops, with 350Gy dosage
60co gamma-ray irradiation, produces an irradiation generation (M
1) seed.
(3) field planting 1.An irradiation generation (M
1) seed, presoaking and germinating, strong seedling is cultivated in light seeding (seeding quantity 15 kgs/acre), this plantation single, and after ripe, mixed receipts produces irradiation two generation (M
2) seed.
(4) resistant plant 1 is screened.M
2for seed sowing, during the rice shoot 3 leaf phase, ponding between drained field, after seedling plate crazing, blend into concentration with 95% glyphosate pulvis is that the aqua even spraying of 100mg/L is on rice shoot.After 2 weeks, rice shoot lobus cardiacus starts leaf roll, gradually withered and yellow death.Only a few seedling growth is only had to be obstructed, but restoration ecosystem gradually after rehydration, until ripe, mixed receipts produces M
3for seed.
(5) field planting 2.With the M that the method propagation steps (4) of step (3) is gathered in the crops
3for seed, produce M
4for seed.
(6) mutagenesis.The M that clear water soaking step (5) is gathered in the crops
4for seed 5 hours, with the chemical mutagen methyl yellow acid second fat (Ethylmethanesulfphonate of 1% concentration, be called for short EMS) process 11 hours, outwell EMS treatment fluid, running water 5 hours, then carries out conventional presoaking and germinating, and strong seedling is cultivated in light seeding (seeding quantity 15 kgs/acre), this transplanting single, mixed receipts EMS after ripe
1for seed.With the method breeding EMS of step (3)
1seed, produces EMS
2for seed.
(7) resistant plant 2 is screened: the EMS that step (6) is gathered in the crops
2for seed, strong seedling is cultivated in light seeding (seeding quantity 15 kgs/acre), and ponding between drained field during tri-leaf period, after seedling plate crazing, blend into concentration with 95% glyphosate pulvis is that the aqua even spraying of 200mg/L is on rice shoot.After 2 weeks, rice shoot lobus cardiacus starts leaf roll, gradually withered and yellow death.Only a few seedling growth is only had to be obstructed, but restoration ecosystem gradually after rehydration, until ripe, individual plant results EMS
3for seed.
(8) combo is hybridized.The EMS that step (7) is gathered in the crops
3for seed, individual plant is numbered, light seeding seedling, and this transplanting single, hybridizes combo between individual plant after heading between two, or three hand over, or multiple cross, produces F
1for hybrid seed.
(9) field planting 3.The F that step (8) is gathered in the crops
1for seed, strong seedling is cultivated in light seeding, this transplanting single, and after ripe, mixed receipts produces F
2for seed.
(10) resistant plant 3 is screened: the F that step (9) is gathered in the crops
2for seed, strong seedling is cultivated in light seeding (seeding quantity 15 kgs/acre), and ponding between drained field during tri-leaf period, after seedling plate crazing, blend into concentration with 95% glyphosate pulvis is that the aqua even spraying of 250mg/L is on rice shoot.After 2 weeks, rice shoot lobus cardiacus starts leaf roll, gradually withered and yellow death.Only a few seedling growth is only had to be obstructed, but restoration ecosystem gradually after rehydration, until ripe, individual plant results F
3for seed.
The F that step (10) is gathered in the crops
3for seed, be glyphosate tolerant weed killer herbicide paddy rice new germ plasm of the present invention, the new rice variety that can be used for glyphosate tolerant weed killer herbicide is cultivated.
Main feature of the present invention is: utilize the method that irradiation mutagenesis and mutagenesis combine, initiative glyphosate tolerant weed killer herbicide paddy rice new germ plasm, then be polymerized the resistant gene of each glyphosate tolerant plant by the method for hybridization combo, cultivate germ plasm resource glyphosate being had to stronger resistance.Compared to other correlation technique current, what this method was formulated is non-transgenic kind matter, has certain resistance to glyphosate herbicidal, can reduce the harm that peasant may cause rice plant because using glyphosate herbicidal improper when rice field and rice field periphery weeding.
The glyphosate tolerant weed killer herbicide paddy rice new germ plasm that the present invention obtains has following beneficial features:
(1) non-transgenic.Resistant gene to be combined generation by irradiation mutagenesis and mutagenesis, is not genetically modified.
(2) stronger patience is had to glyphosate herbicidal.First with irradiation mutagenesis method initiative resistant plant, then strengthen plant resistance with chemical mutagenesis, the method finally by hybridization condenses together the resistant gene of separate sources, reaches object glyphosate herbicidal being had to stronger resistance.
(3) use safety.Because the rice plant of the method initiative has certain resistance to glyphosate herbicidal, during the spray such as rice field periphery, ridge Gyphosate herbicice, be sprayed on rice seedling on a small quantity even if having, also can not cause serious injury to rice seedling.
(4) easy to use.Because glyphosate herbicidal is the raw type weed killer herbicide that goes out, have kill activity to large field weed, easy to use, cost is low.
Embodiment
embodiment 1
(1) Zhejiang round-grained rice 22(New species right in plant CNA20070398.6) be the late japonica rice new varieties of Zhejiang Academy of Agricultural Science crop and the seed selection of nuclear technique research on utilization institute, 2006 by Zhejiang Province's variety certification (rice 2006013 is examined in Zhejiang).This kind has the advantages that output is high, resistance, rice are of fine quality, but responsive to glyphosate herbicidal.When Zhejiang Province's large scale application, usually because of peasant household on ridge, spray Gyphosate herbicice time, Zhejiang round-grained rice 22 plant occurred near balk is caused damage by poisoning.For the resistance of improvement Zhejiang round-grained rice 22 pairs of glyphosate herbicidals, 350Gy dosage
60co gamma-ray irradiation Zhejiang round-grained rice 22 breeder's stock 10 kilograms, light seeding is in one piece of 300M
2experimental field in, cultivate strong seedling, be transplanted to two pieces of 4000M altogether
2experimental field, mixed receive M
1for the M on plant
2500 kilograms, seed.
(2) M
2seed, light seeding is total to 13340M in 8 pieces
2seedling plate on, during three leaves, between drained field, ponding has tiny cracking to seedling plate, sprays 95% glyphosate pulvis and blends into the gyphosate solution that concentration is 100mg/L, and rehydration supports seedling, diseases prevention insect protected after 2 weeks, until ripe, the M that mixed harvest is lived in strain
35 kilograms, seed.
(3) M
3seed, 1% concentration chemical mutagen methyl yellow acid second fat (Ethylmethanesulfphonate is called for short EMS) processes 11 hours, and outwell EMS treatment fluid, running water 5 hours, then carries out conventional presoaking and germinating, and light seeding is in one piece of 100M
2experimental field in, cultivate strong seedling, this transplantings single, diseases prevention insect protected, mixedly receive generation EMS
1for 50 kilograms, seed.Breeding EMS
1for seed, produce EMS
2for 500 kilograms, seed.
(4) EMS
2seed, light seeding is total to 13340M in 8 pieces
2seedling plate on, during three leaves, between drained field, ponding has tiny cracking to seedling plate, sprays 95% glyphosate pulvis and blends into the gyphosate solution that concentration is 200mg/L, and rehydration supports seedling, diseases prevention insect protected after 2 weeks, until ripe, individual plant gathers in the crops 20 EMS survived in strain
3seed.Numbering EMS
3-1, EMS
3-2, EMS
3-3, EMS
3-4, EMS
3-5 ..., EMS
3-20.
(5) EMS
3-1, EMS
3-2, EMS
3-3, EMS
3-4, EMS
3-5 seeds such as grade, strong seedling is cultivated in light seeding, and this transplanting single, hybridizes combo, i.e. EMS when heading is bloomed between two
3-1 × EMS
3-2, EMS
3-1 × EMS
3-3, EMS
3-1 × EMS
3-4, EMS
3-1 × EMS
3-5, EMS
3-2 × EMS
3-3, EMS
3-2 × EMS
3-4, EMS
3-2 × EMS
3-5, EMS
3-3 × EMS
3-4, EMS
3-3 × EMS
3-5, EMS
3-4 × EMS
3-5 etc., obtain F
1hybrid seed 55 combination.
The F of (6) 55 combinations
1seed, strong seedling is cultivated in light seeding, this transplanting single, diseases prevention insect protected, mixed receipts F
1f on plant
2seed.
The F of (7) 55 combinations
2seed, often combine the seed of light seeding about 1000 on seedling plate, during rice shoot three leaf, between drained field, ponding has tiny cracking to seedling plate, sprays 95% glyphosate pulvis and blends into the gyphosate solution that concentration is 250mg/L, and after 2 weeks, rehydration supports seedling, and results are 5 F combined wherein
2survive the F in strain
3seed.
The F of (8) 5 combinations
3and above each for seed, this kind single becomes strain, until proterties is stablized, is not separated plant and occurs.
The Zhejiang round-grained rice 22 physics and chemistry behavior progeny material obtained by above method, is had the characteristic of glyphosate tolerant weed killer herbicide, is glyphosate tolerant paddy rice new germ plasm.
embodiment 2
(1) identical to step (5) with the step (1) of embodiment 1, the EMS of acquisition
3-1, EMS
3-2, EMS
3-3, EMS
3-4, EMS
3strong seedling is cultivated in-5 seed light seedings, and this transplanting single, hybridizes combo, i.e. EMS when heading is bloomed between two
3-1 × EMS
3-2, EMS
3-1 × EMS
3-3, EMS
3-1 × EMS
3-4, EMS
3-1 × EMS
3-5, EMS
3-2 × EMS
3-3, EMS
3-2 × EMS
3-4, EMS
3-2 × EMS
3-5, EMS
3-3 × EMS
3-4, EMS
3-3 × EMS
3-5, EMS
3-4 × EMS
3-5, results F
1hybrid seed 55 combination.
The F of (2) 55 combinations
1seed, strong seedling is cultivated in light seeding, this transplanting single, diseases prevention insect protected, EMS during heading
3-1 × EMS
3the F of-2
1with EMS
3-1 × EMS
3the F of-3
1phase mutual cross, forms multiple cross combination (EMS
3-1 × EMS
3-2) × (EMS
3-1 × EMS
3, and obtain new F-3)
1.
(3) identical to step (8) with the step (6) of embodiment 1, Zhejiang round-grained rice 22 progeny material obtained by above method, is had and has better patience to glyphosate, is glyphosate tolerant paddy rice new germ plasm.
Claims (1)
1. a breeding method for glyphosate tolerant weed killer herbicide paddy rice new germ plasm, is characterized in that the method comprises the following steps:
1) seed preparation
Select the conventional Rice govern-house-variety of local spread, seedling is strengthened in light seeding, this plantation single, and individual plant is gathered in the crops;
2) irradiation mutagenesis
The dry seeds of step 1) results, with 350Gy dosage
60co gamma-ray irradiation, produces irradiation generation M
1seed;
3) field planting one
Irradiation generation M
1seed, presoaking and germinating, strong seedling is cultivated in light seeding, this plantation single, and after ripe, mixed receipts produces irradiation two generation M
2seed;
4) resistant plant one is screened
M
2for seed sowing, during the rice shoot 3 leaf phase, ponding between drained field, after seedling plate crazing, blend into concentration with 95% glyphosate pulvis is that the aqua even spraying of 100mg/L is on rice shoot; After 2 weeks, rice shoot lobus cardiacus starts leaf roll, gradually withered and yellow death; Only a few seedling growth is only had to be obstructed, but restoration ecosystem gradually after rehydration, until ripe, mixed receipts produces M
3for seed;
5) field planting two
M
3for seed, presoaking and germinating, strong seedling is cultivated in light seeding, this plantation single, and after ripe, mixed receipts produces M
4seed;
6) mutagenesis
Clear water soaking step 5) M that gathers in the crops
4for seed 5 hours, with the chemical mutagen methyl yellow acid second fat process 11 hours of 1% concentration, outwell EMS treatment fluid, running water 5 hours, then carried out conventional presoaking and germinating, and strong seedling is cultivated in light seeding, this transplantings single, mixed receipts EMS after ripe
1for seed; EMS
1for seed, presoaking and germinating, strong seedling is cultivated in light seeding, this plantation single, and after ripe, mixed receipts produces EMS
2for seed;
7) resistant plant two is screened
The EMS of step 6) results
2for seed, strong seedling is cultivated in light seeding, ponding between drained field during tri-leaf period, and after seedling plate crazing, blend into concentration with 95% glyphosate pulvis is that the aqua even spraying of 200mg/L is on rice shoot; After 2 weeks, rice shoot lobus cardiacus starts leaf roll, gradually withered and yellow death; Only a few seedling growth is only had to be obstructed, but restoration ecosystem gradually after rehydration, until ripe, individual plant results EMS
3for seed;
8) combo is hybridized
The EMS of step 7) results
3for seed, individual plant is numbered, light seeding seedling, and this transplanting single, hybridizes combo between individual plant after heading between two, or three hand over, or multiple cross, produces F
1for hybrid seed;
9) field planting three
The F of step 8) results
1for seed, strong seedling is cultivated in light seeding, this transplanting single, and after ripe, mixed receipts produces F
2for seed;
10) resistant plant three is screened
The F of step 9) results
2for seed, strong seedling is cultivated in light seeding, ponding between drained field during tri-leaf period, and after seedling plate crazing, blend into concentration with 95% glyphosate pulvis is that the aqua even spraying of 250mg/L is on rice shoot; After 2 weeks, rice shoot lobus cardiacus starts leaf roll, gradually withered and yellow death; Only a few seedling growth is only had to be obstructed, but restoration ecosystem gradually after rehydration, until ripe, individual plant results F
3for seed;
The F of step 10) results
3for seed, be described glyphosate tolerant weed killer herbicide paddy rice new germ plasm.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107211888A (en) * | 2017-07-07 | 2017-09-29 | 江苏徐淮地区淮阴农业科学研究所 | A kind of easy screening or the method for identifying anti-AC 263222 paddy rice |
| CN107242021A (en) * | 2017-08-03 | 2017-10-13 | 黑龙江省科学院大庆分院 | The radiation breeding method of alfalfa |
| CN108040870A (en) * | 2017-12-21 | 2018-05-18 | 贵州大学 | A kind of screening technique of endogenous glyphosate-resistant rice and endogenous EPSPS genes |
| CN109122138A (en) * | 2018-08-09 | 2019-01-04 | 福建省农业科学院生物技术研究所 | A kind of method for directly seeding rice based on glyphosate tolerant |
| CN116267595A (en) * | 2023-04-07 | 2023-06-23 | 浙江省农业科学院 | Method for judging recommended irradiation dose for crop mutation breeding |
| CN116267595B (en) * | 2023-04-07 | 2024-05-24 | 浙江省农业科学院 | Method for judging recommended irradiation dose for crop mutation breeding |
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2015
- 2015-08-19 CN CN201510510087.1A patent/CN105230480A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107211888A (en) * | 2017-07-07 | 2017-09-29 | 江苏徐淮地区淮阴农业科学研究所 | A kind of easy screening or the method for identifying anti-AC 263222 paddy rice |
| CN107242021A (en) * | 2017-08-03 | 2017-10-13 | 黑龙江省科学院大庆分院 | The radiation breeding method of alfalfa |
| CN107242021B (en) * | 2017-08-03 | 2020-06-02 | 黑龙江省科学院大庆分院 | Radiation cultivation method of alfalfa |
| CN108040870A (en) * | 2017-12-21 | 2018-05-18 | 贵州大学 | A kind of screening technique of endogenous glyphosate-resistant rice and endogenous EPSPS genes |
| CN109122138A (en) * | 2018-08-09 | 2019-01-04 | 福建省农业科学院生物技术研究所 | A kind of method for directly seeding rice based on glyphosate tolerant |
| CN116267595A (en) * | 2023-04-07 | 2023-06-23 | 浙江省农业科学院 | Method for judging recommended irradiation dose for crop mutation breeding |
| CN116267595B (en) * | 2023-04-07 | 2024-05-24 | 浙江省农业科学院 | Method for judging recommended irradiation dose for crop mutation breeding |
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Application publication date: 20160113 |