CN107211888A - A kind of easy screening or the method for identifying anti-AC 263222 paddy rice - Google Patents
A kind of easy screening or the method for identifying anti-AC 263222 paddy rice Download PDFInfo
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- CN107211888A CN107211888A CN201710567939.XA CN201710567939A CN107211888A CN 107211888 A CN107211888 A CN 107211888A CN 201710567939 A CN201710567939 A CN 201710567939A CN 107211888 A CN107211888 A CN 107211888A
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- rice
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- paddy rice
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- Life Sciences & Earth Sciences (AREA)
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- Developmental Biology & Embryology (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of easy screening or the method for identifying anti-AC 263222 paddy rice, comprise the following steps：Choose the ridge of herbicide hundred to lead to, be diluted with water to AC 263222 concentration for 0.1ml/L, the dry seed of experimental cultivar is loaded into water imbibition sulfuric acid paper bag or gauze bag, immerses after dilution, 48h and takes out；Seed soaking is used after flushing, takes out after 24h and drains away the water, being uniformly seeded in tiling has in the plastics cave dish of 1cm rice nursery substrates, nonwatertight moisture keeping is poured after covering 1cm same matrix, progress observation statistics after 22 30 DEG C, 57 days of room temperature is kept.The present invention with the screening of simple and efficient efficiently and accurately or can identify the paddy rice of antiweed AC 263222, and good technological means is provided for the rice breeding of antiweed AC 263222.
Description
Technical field
The present invention relates to rice breeding field, and in particular to a kind of easy screening or the side for identifying anti-AC 263222 paddy rice
Method.
Background technology
Paddy rice is the staple food crop that global population more than half is depended on for existence, is had to human lives and grain security
Significance.China's rice cultivation is with a long history, and region is wide, is one of View of World Rice main production country.Per Annual planting area about
35000000 hm2, account for the 29% of cereal crops sown area.In the rice field of all rice workspaces of China, always there are a large amount of weeds
Occur.According to statistics, national weeds in paddy field has more than 200 kinds, wherein occurring universal, serious, most common weeds of causing harm there are about 40 kinds
(being distributed in each rice region for 10~20 kinds).According to investigations, national rice field crop smothering more than medium (2~5 grades) area up to 1546.7
Ten thousand hm2, wherein (4~5 grades) area of seriously causing harm is 3,800,000 hm2, the 46.8% and 11.5% of Monitoring of Paddy Rice Plant Area is accounted for respectively, is damaged
Lose the kg of grain more than 200 hundred million.Farmland weed and crop contention nutriment, moisture, sunlight and space, hinder field ventilation and penetrating light, increase
Mesoclimate temperature, some are then disease pest vectors, promote pest and disease damage；Parasitics weeds are directly from crop body absorption
Nutrient, so as to reduce the yield and quality of crop.In addition, the seed or pollen of some weeds contain toxin, person poultry poisoning can be made.
In recent ten years, Weedy Rice turn into Rice Production in a kind of new crop smothering, be hybrid rice offspring or affiliation farther out
Rice Progenies formed by nature and artificial selection, with early germination, early tiller, morning heading, early ripe, Yi Luo
The features such as grain, rice matter are poor, rest period elasticity is big, procreation speed is fast and are difficult to pull out.At present, Weedy Rice has become global
Crop smothering, in some countries, Weedy Rice causes paddy rice underproduction 10-15%.Weedy Rice is actually occurred in China, rice growing area
Area accounts for 20%, and yield average loss is 10% or so, and annual loss quenched 10,000,000,000 yuan, is referred to as paddy rice killer.Therefore, prevent
Rice of cutting weeds turns into urgent problem to be solved in current Rice Production, has important meaning for the yield and quality for improving paddy rice
Justice.
There are manual uprooting weed, farm implements weeding and chemical weed control to weeds in paddy field and preventing and kill off mainly for Weedy Rice.Manual uprooting weed and
Farm implements weeding effort is more, work efficiency is low, it is impossible to which large area is prevented and kill off in time.Therefore, weeds in field and Weedy Rice are prevented and kill off using herbicide
It is a most effective approach.Acetolactate synthestase (ALS) is the primary key enzyme in branched-chain amino acid route of synthesis, is made with it
It is divided into for the herbicide of target：Sulfonylurea, imidazolone type, pyrimidine salicylic acid and sulfonamides.This kind of herbicide has
The features such as high activity, herbicide wide spectrum and strong selectivity, be current preventing and treating weeds in paddy field, ratooning rice, the comparatively ideal weeding of Weedy Rice
Agent.AC 263222 is a kind of strong absorbability imidazolinone herbicide, and weeds aerial part plant can not only be killed well,
And also have stronger preventive effect to the root and subterranean stem of underground part.
At present, the resistance screening of rice herbicide or identification are mainly carried out by field test, i.e., in field to paddy rice
Sowed, spraying for herbicide is carried out after the regular period.The method is wasted time and energy, and the concentration and the uniformity that spray are difficult
Control, so as to have impact on the accuracy of result.And use the method cost of molecular labeling higher, and sport technique segment is more.Therefore one
The antiweed screening or authentication method for planting quickly, efficiently and accurately are just particularly important.
The content of the invention
To solve the above problems, the invention provides a kind of easy screening or the method for identifying anti-AC 263222 paddy rice,
Only with steps such as seed soaking, hole tray sowing, data statistics, you can screen or identify resistant variety.
To achieve the above object, the technical scheme taken of the present invention is：
A kind of easy screening or the method for identifying anti-AC 263222 paddy rice, it is characterised in that comprise the following steps：
S1, choose the ridge of herbicide hundred and lead to, AC 263222 concentration is diluted with water to for 0.1ml/L, by the dry seed of experimental cultivar
Grain loads water imbibition sulfuric acid paper bag or gauze bag, immerses after dilution, 48h and takes out；
Use to take out after Seed soaking, 24h after S2, flushing and drain away the water, being uniformly seeded in tiling there are 1cm rice nursery substrates
Plastics cave dish in, pour nonwatertight moisture keeping after covering 1cm same matrix, keep progress observation statistics after 22-30 DEG C, 5-7 days of room temperature,
Specifically, the germination percentage of investigation experimental cultivar, i.e. germinative number account for the percentage of test seed sum；The paddy rice of anti-AC 263222
Kind, its germination percentage is not influenceed by seed-soaking liquid, under normal circumstances should be more than 85%；Without the product of anti-AC 263222
Kind, the plumule in its seed is suppressed by seed-soaking liquid, and germination percentage is less than 10%
Wherein, the size of the plastics cave dish is 30cm × 60cm.
The invention has the advantages that：
The present invention with the screening of simple and efficient efficiently and accurately or can identify the paddy rice of antiweed AC 263222, be anti-
The rice breeding of herbicide AC 263222 provides good technological means.
Embodiment
In order that objects and advantages of the present invention are more clearly understood, the present invention is carried out with reference to embodiments further
Describe in detail.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to limit this hair
It is bright.
Embodiment
Experimental cultivar is the japonica rice variety (Huaihe River rice No. 5, Huaihe River rice 119) and three anti-AC 263222s of 2 not antiweeds
New germ plasm (T1, T2, T3).Each kind selects full 2 parts of seed rice, 100 every part, loads white sulfuric acid paper bag, uses stapler
Or clip sealing.Portion immersion herbicide AC 263222 dilution, another is compared with Seed soaking.Treat sowing budding
Statistics germination percentage (table 1), as a result shows afterwards, after being soaked seed with AC 263222 dilution, the germination percentage of tri- parts of germplasm of T1, T2, T3
More than 85%, germination percentage does not have difference afterwards with Seed soaking (CK)；Huaihe River rice No. 5 and Huaihe River rice 119 two kinds first imidazoles cigarette
Germination percentage is respectively 2% and 0% after acidleach kind, and it compares germination percentage and respectively reaches 96% and 95%.Show antiweed kind
Do not influenceed by AC 263222, and Huaihe River rice No. 5 and Huaihe River rice 119 are influenceed huge by herbicide seed-soaking liquid, its germinating power is pressed down completely
System.The method is used to screening and identifying anti-AC 263222 rice varieties, has the advantages that safe and efficient precise and high efficiency, Ke Yiyong
In the seed selection and plantation of antiweed kind, with wide market prospects and application value.
Germination percentage (%) after the experimental cultivar herbicide of table 1 and Seed soaking
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (3)
1. a kind of easy screening or the method for identifying anti-AC 263222 paddy rice, it is characterised in that comprise the following steps：
S1, choose the ridge of herbicide hundred and lead to, be diluted with water to AC 263222 concentration for 0.1ml/L, the dry seed of experimental cultivar is filled
Enter water imbibition sulfuric acid paper bag or gauze bag, immerse after dilution, 48h and take out；
Use to take out after Seed soaking, 24h after S2, flushing and drain away the water, being uniformly seeded in tiling has the modeling of 1cm rice nursery substrates
In material cave disk, nonwatertight moisture keeping is poured after covering 1cm same matrix, progress observation statistics after 22-30 DEG C, 5-7 days of room temperature is kept.
2. a kind of easy screening as claimed in claim 1 or the method for identifying anti-AC 263222 paddy rice, it is characterised in that institute
The size for stating plastics cave dish is 30cm × 60cm.
3. a kind of easy screening as claimed in claim 1 or the method for identifying anti-AC 263222 paddy rice, it is characterised in that institute
The SS for stating step S2 is；Investigate the percentage that the germination percentage of experimental cultivar, i.e. germinative number account for test seed sum；Anti- first
The rice varieties of imazapyr, its germination percentage is not influenceed by seed-soaking liquid, under normal circumstances should be more than 85%；Without anti-first
Plumule in the kind of imazapyr, its seed is suppressed by seed-soaking liquid, and germination percentage is less than 10%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710567939.XA CN107211888A (en) | 2017-07-07 | 2017-07-07 | A kind of easy screening or the method for identifying anti-AC 263222 paddy rice |
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| CN201710567939.XA CN107211888A (en) | 2017-07-07 | 2017-07-07 | A kind of easy screening or the method for identifying anti-AC 263222 paddy rice |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111631132A (en) * | 2020-07-16 | 2020-09-08 | 黑龙江省农业科学院绥化分院 | Breeding method of herbicide-resistant dry-planting rice variety |
| CN112106649A (en) * | 2020-09-16 | 2020-12-22 | 河北省农林科学院滨海农业研究所 | Rapid breeding method of imidazolinone herbicide-resistant rice germplasm |
Citations (3)
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|---|---|---|---|---|
| CN1582639A (en) * | 2004-05-26 | 2005-02-23 | 中国科学院亚热带农业生态研究所 | Method for mutagenizing anti-chlorosulfuron somatic mutant of rice |
| CN102946715A (en) * | 2010-01-07 | 2013-02-27 | 巴斯福农业有限责任公司阿纳姆(荷兰)韦登斯维尔分公司 | Herbicide-tolerant plants |
| CN105230480A (en) * | 2015-08-19 | 2016-01-13 | 浙江省农业科学院 | Breeding method for novel glyphosate-herbicide-resistant rice germplasm |
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2017
- 2017-07-07 CN CN201710567939.XA patent/CN107211888A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1582639A (en) * | 2004-05-26 | 2005-02-23 | 中国科学院亚热带农业生态研究所 | Method for mutagenizing anti-chlorosulfuron somatic mutant of rice |
| CN102946715A (en) * | 2010-01-07 | 2013-02-27 | 巴斯福农业有限责任公司阿纳姆(荷兰)韦登斯维尔分公司 | Herbicide-tolerant plants |
| CN105230480A (en) * | 2015-08-19 | 2016-01-13 | 浙江省农业科学院 | Breeding method for novel glyphosate-herbicide-resistant rice germplasm |
Non-Patent Citations (3)
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| 宋鹏等: "不同诱变方法对抗咪唑乙烟酸水稻筛选的影响", 《沈阳农业大学学报》 * |
| 王建平等: "抗咪唑啉酮水稻对普施特除草剂的抗性效应", 《江西农业学报》 * |
| 苏少泉: "乙酰乳酸合成酶抑制型除草剂抗性作物的创制与发展", 《世界农药》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111631132A (en) * | 2020-07-16 | 2020-09-08 | 黑龙江省农业科学院绥化分院 | Breeding method of herbicide-resistant dry-planting rice variety |
| CN112106649A (en) * | 2020-09-16 | 2020-12-22 | 河北省农林科学院滨海农业研究所 | Rapid breeding method of imidazolinone herbicide-resistant rice germplasm |
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Application publication date: 20170929 |
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