EP3643775B1 - Bacillus amyloliquefaciens qv15 stimulant du métabolisme secondaire de composés phénoliques et de la capacité inhibitrice des extraits de framboise et de fraise sur les enzymes associés au syndrome métabolique - Google Patents
Bacillus amyloliquefaciens qv15 stimulant du métabolisme secondaire de composés phénoliques et de la capacité inhibitrice des extraits de framboise et de fraise sur les enzymes associés au syndrome métabolique Download PDFInfo
- Publication number
- EP3643775B1 EP3643775B1 EP18820445.7A EP18820445A EP3643775B1 EP 3643775 B1 EP3643775 B1 EP 3643775B1 EP 18820445 A EP18820445 A EP 18820445A EP 3643775 B1 EP3643775 B1 EP 3643775B1
- Authority
- EP
- European Patent Office
- Prior art keywords
- bacillus amyloliquefaciens
- cect
- deposited
- raspberry
- extracts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000000284 extract Substances 0.000 title claims description 46
- 241000193744 Bacillus amyloliquefaciens Species 0.000 title claims description 31
- 150000002989 phenols Chemical class 0.000 title claims description 28
- 235000016623 Fragaria vesca Nutrition 0.000 title claims description 20
- 235000009122 Rubus idaeus Nutrition 0.000 title claims description 18
- 235000011363 Fragaria x ananassa Nutrition 0.000 title claims description 16
- 230000024053 secondary metabolic process Effects 0.000 title claims description 16
- 235000011034 Rubus glaucus Nutrition 0.000 title claims description 15
- 102000004190 Enzymes Human genes 0.000 title claims description 13
- 108090000790 Enzymes Proteins 0.000 title claims description 13
- 208000001145 Metabolic Syndrome Diseases 0.000 title claims description 12
- 201000000690 abdominal obesity-metabolic syndrome Diseases 0.000 title claims description 12
- 240000009088 Fragaria x ananassa Species 0.000 title claims 4
- 244000235659 Rubus idaeus Species 0.000 title description 21
- 230000002401 inhibitory effect Effects 0.000 title description 4
- 241000196324 Embryophyta Species 0.000 claims description 48
- 235000010208 anthocyanin Nutrition 0.000 claims description 36
- 229930002877 anthocyanin Natural products 0.000 claims description 36
- 239000004410 anthocyanin Substances 0.000 claims description 36
- 150000004636 anthocyanins Chemical class 0.000 claims description 36
- 235000013399 edible fruits Nutrition 0.000 claims description 28
- 101000605127 Homo sapiens Prostaglandin G/H synthase 2 Proteins 0.000 claims description 26
- 102100024295 Maltase-glucoamylase Human genes 0.000 claims description 26
- 108010028144 alpha-Glucosidases Proteins 0.000 claims description 26
- 101000725401 Homo sapiens Cytochrome c oxidase subunit 2 Proteins 0.000 claims description 22
- 241001092459 Rubus Species 0.000 claims description 19
- 241000894006 Bacteria Species 0.000 claims description 18
- 230000000694 effects Effects 0.000 claims description 17
- 235000021029 blackberry Nutrition 0.000 claims description 14
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 12
- 235000016709 nutrition Nutrition 0.000 claims description 11
- 235000017848 Rubus fruticosus Nutrition 0.000 claims description 10
- 230000000975 bioactive effect Effects 0.000 claims description 9
- 229930003935 flavonoid Natural products 0.000 claims description 8
- 150000002215 flavonoids Chemical class 0.000 claims description 8
- 235000017173 flavonoids Nutrition 0.000 claims description 8
- 230000000144 pharmacologic effect Effects 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 7
- 244000078534 Vaccinium myrtillus Species 0.000 claims description 6
- 244000005700 microbiome Species 0.000 claims description 6
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 claims description 5
- 235000005487 catechin Nutrition 0.000 claims description 5
- 206010020772 Hypertension Diseases 0.000 claims description 4
- 206010061218 Inflammation Diseases 0.000 claims description 4
- 235000017537 Vaccinium myrtillus Nutrition 0.000 claims description 4
- 230000004054 inflammatory process Effects 0.000 claims description 4
- 150000001765 catechin Chemical class 0.000 claims description 3
- 238000004040 coloring Methods 0.000 claims description 3
- 241000093727 Berzelia alopecuroides Species 0.000 claims description 2
- 235000011552 Rhamnus crocea Nutrition 0.000 claims description 2
- 235000003095 Vaccinium corymbosum Nutrition 0.000 claims description 2
- 235000021014 blueberries Nutrition 0.000 claims description 2
- 238000000338 in vitro Methods 0.000 claims description 2
- 240000007651 Rubus glaucus Species 0.000 claims 3
- 102100027456 Cytochrome c oxidase subunit 2 Human genes 0.000 claims 2
- 102100030988 Angiotensin-converting enzyme Human genes 0.000 claims 1
- 101710185050 Angiotensin-converting enzyme Proteins 0.000 claims 1
- 102000015427 Angiotensins Human genes 0.000 claims 1
- 108010064733 Angiotensins Proteins 0.000 claims 1
- 241000219094 Vitaceae Species 0.000 claims 1
- 229940125690 blood glucose regulator Drugs 0.000 claims 1
- 230000004069 differentiation Effects 0.000 claims 1
- 235000021021 grapes Nutrition 0.000 claims 1
- 239000003112 inhibitor Substances 0.000 claims 1
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 21
- HVQAJTFOCKOKIN-UHFFFAOYSA-N flavonol Natural products O1C2=CC=CC=C2C(=O)C(O)=C1C1=CC=CC=C1 HVQAJTFOCKOKIN-UHFFFAOYSA-N 0.000 description 21
- 235000011957 flavonols Nutrition 0.000 description 21
- 102100038280 Prostaglandin G/H synthase 2 Human genes 0.000 description 20
- 241000220223 Fragaria Species 0.000 description 19
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 18
- 150000002216 flavonol derivatives Chemical class 0.000 description 16
- 238000004519 manufacturing process Methods 0.000 description 14
- 238000005259 measurement Methods 0.000 description 13
- 229940088598 enzyme Drugs 0.000 description 11
- 230000007423 decrease Effects 0.000 description 10
- 230000007123 defense Effects 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 10
- 235000013305 food Nutrition 0.000 description 10
- 230000005764 inhibitory process Effects 0.000 description 10
- 230000029553 photosynthesis Effects 0.000 description 10
- 238000010672 photosynthesis Methods 0.000 description 10
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 8
- 235000013824 polyphenols Nutrition 0.000 description 8
- 230000001580 bacterial effect Effects 0.000 description 7
- 238000012512 characterization method Methods 0.000 description 7
- 229940068517 fruit extracts Drugs 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- -1 ACE Proteins 0.000 description 6
- 102000012286 Chitinases Human genes 0.000 description 6
- 108010022172 Chitinases Proteins 0.000 description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 238000004737 colorimetric analysis Methods 0.000 description 6
- 230000007246 mechanism Effects 0.000 description 6
- 230000004060 metabolic process Effects 0.000 description 6
- 230000002265 prevention Effects 0.000 description 6
- 239000003963 antioxidant agent Substances 0.000 description 5
- 230000003078 antioxidant effect Effects 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 5
- 238000000605 extraction Methods 0.000 description 5
- 150000007946 flavonol Chemical class 0.000 description 5
- 244000052769 pathogen Species 0.000 description 5
- 230000001717 pathogenic effect Effects 0.000 description 5
- 230000037361 pathway Effects 0.000 description 5
- 239000001967 plate count agar Substances 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 235000021013 raspberries Nutrition 0.000 description 5
- 235000021012 strawberries Nutrition 0.000 description 5
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 4
- 244000307700 Fragaria vesca Species 0.000 description 4
- VSCWAEJMTAWNJL-UHFFFAOYSA-K aluminium trichloride Chemical compound Cl[Al](Cl)Cl VSCWAEJMTAWNJL-UHFFFAOYSA-K 0.000 description 4
- 235000006708 antioxidants Nutrition 0.000 description 4
- 235000021028 berry Nutrition 0.000 description 4
- 230000006696 biosynthetic metabolic pathway Effects 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 230000036541 health Effects 0.000 description 4
- 230000006872 improvement Effects 0.000 description 4
- 239000000419 plant extract Substances 0.000 description 4
- 230000002000 scavenging effect Effects 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 208000024891 symptom Diseases 0.000 description 4
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 108020004465 16S ribosomal RNA Proteins 0.000 description 3
- 102100037373 DNA-(apurinic or apyrimidinic site) endonuclease Human genes 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 206010033307 Overweight Diseases 0.000 description 3
- 239000000589 Siderophore Substances 0.000 description 3
- 108091023040 Transcription factor Proteins 0.000 description 3
- 102000040945 Transcription factor Human genes 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 239000013543 active substance Substances 0.000 description 3
- 230000009418 agronomic effect Effects 0.000 description 3
- 102000004139 alpha-Amylases Human genes 0.000 description 3
- 108090000637 alpha-Amylases Proteins 0.000 description 3
- 229940024171 alpha-amylase Drugs 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 230000033228 biological regulation Effects 0.000 description 3
- 229930002875 chlorophyll Natural products 0.000 description 3
- 235000019804 chlorophyll Nutrition 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 230000014509 gene expression Effects 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 230000002503 metabolic effect Effects 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 230000008659 phytopathology Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 229930013915 (+)-catechin Natural products 0.000 description 2
- 235000007219 (+)-catechin Nutrition 0.000 description 2
- 241000219195 Arabidopsis thaliana Species 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 2
- 244000045195 Cicer arietinum Species 0.000 description 2
- 235000010523 Cicer arietinum Nutrition 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 2
- 238000007400 DNA extraction Methods 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 241000543691 Lactarius deliciosus Species 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 240000000249 Morus alba Species 0.000 description 2
- 235000008708 Morus alba Nutrition 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 102100034681 Myeloblastin Human genes 0.000 description 2
- 235000008575 Pinus pinea Nutrition 0.000 description 2
- 240000007789 Pinus pinea Species 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 239000012445 acidic reagent Substances 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000000090 biomarker Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 2
- 239000001752 chlorophylls and chlorophyllins Substances 0.000 description 2
- 229950001002 cianidanol Drugs 0.000 description 2
- 206010012601 diabetes mellitus Diseases 0.000 description 2
- 150000002085 enols Chemical class 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 229940074391 gallic acid Drugs 0.000 description 2
- 235000004515 gallic acid Nutrition 0.000 description 2
- 238000012252 genetic analysis Methods 0.000 description 2
- 230000007407 health benefit Effects 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 230000001771 impaired effect Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000006799 invasive growth in response to glucose limitation Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 230000036542 oxidative stress Effects 0.000 description 2
- 229930015704 phenylpropanoid Natural products 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 230000019525 primary metabolic process Effects 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 230000036186 satiety Effects 0.000 description 2
- 235000019627 satiety Nutrition 0.000 description 2
- 229930000044 secondary metabolite Natural products 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 238000004611 spectroscopical analysis Methods 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 230000035882 stress Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- PAJPWUMXBYXFCZ-UHFFFAOYSA-N 1-aminocyclopropanecarboxylic acid Chemical compound OC(=O)C1(N)CC1 PAJPWUMXBYXFCZ-UHFFFAOYSA-N 0.000 description 1
- 241000223600 Alternaria Species 0.000 description 1
- 101100132358 Arabidopsis thaliana MYB6 gene Proteins 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- 241001112741 Bacillaceae Species 0.000 description 1
- 241000193833 Bacillales Species 0.000 description 1
- 241000304886 Bacilli Species 0.000 description 1
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 description 1
- 108020000946 Bacterial DNA Proteins 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102000005575 Cellulases Human genes 0.000 description 1
- 108010084185 Cellulases Proteins 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102100036816 Eukaryotic peptide chain release factor GTP-binding subunit ERF3A Human genes 0.000 description 1
- 101000906005 Fasciola hepatica Glutathione S-transferase class-mu 26 kDa isozyme 1 Proteins 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- 241000895522 Fusarium oxysporum f. sp. ciceris Species 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 101000851788 Homo sapiens Eukaryotic peptide chain release factor GTP-binding subunit ERF3A Proteins 0.000 description 1
- 101001071694 Homo sapiens Glutathione S-transferase Mu 1 Proteins 0.000 description 1
- 108010028921 Lipopeptides Proteins 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 235000005205 Pinus Nutrition 0.000 description 1
- 241000218602 Pinus <genus> Species 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 241001236212 Pinus pinaster Species 0.000 description 1
- 235000005105 Pinus pinaster Nutrition 0.000 description 1
- 241000589540 Pseudomonas fluorescens Species 0.000 description 1
- 241000259045 Pseudomonas syringae pv. tomato str. DC3000 Species 0.000 description 1
- 108020001027 Ribosomal DNA Proteins 0.000 description 1
- 244000157431 Rubus ellipticus Species 0.000 description 1
- 235000010814 Rubus ellipticus Nutrition 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 241000589636 Xanthomonas campestris Species 0.000 description 1
- 230000011759 adipose tissue development Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000003178 anti-diabetic effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 229940093797 bioflavonoids Drugs 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229930002868 chlorophyll a Natural products 0.000 description 1
- 229930002869 chlorophyll b Natural products 0.000 description 1
- NSMUHPMZFPKNMZ-VBYMZDBQSA-M chlorophyll b Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C=O)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 NSMUHPMZFPKNMZ-VBYMZDBQSA-M 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- YTAQZPGBTPDBPW-UHFFFAOYSA-N flavonoid group Chemical class O1C(C(C(=O)C2=CC=CC=C12)=O)C1=CC=CC=C1 YTAQZPGBTPDBPW-UHFFFAOYSA-N 0.000 description 1
- 244000000049 foliar pathogen Species 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000012268 genome sequencing Methods 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 230000010030 glucose lowering effect Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000007952 growth promoter Substances 0.000 description 1
- 230000003054 hormonal effect Effects 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000000401 methanolic extract Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000626 neurodegenerative effect Effects 0.000 description 1
- 102000042567 non-coding RNA Human genes 0.000 description 1
- 239000006916 nutrient agar Substances 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 244000039328 opportunistic pathogen Species 0.000 description 1
- LCCNCVORNKJIRZ-UHFFFAOYSA-N parathion Chemical compound CCOP(=S)(OCC)OC1=CC=C([N+]([O-])=O)C=C1 LCCNCVORNKJIRZ-UHFFFAOYSA-N 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 150000002995 phenylpropanoid derivatives Chemical class 0.000 description 1
- 125000001474 phenylpropanoid group Chemical group 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 244000000003 plant pathogen Species 0.000 description 1
- 229930001119 polyketide Natural products 0.000 description 1
- 150000003881 polyketide derivatives Chemical class 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 229930010796 primary metabolite Natural products 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 244000000000 soil microbiome Species 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000005063 solubilization Methods 0.000 description 1
- 230000007928 solubilization Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
Definitions
- This invention relates to a Bacillus amyloliquefaciens (QV15, laboratory internal code) strain for application in plant with the aim of improving the synthesis of phenolic compounds of the secondary metabolism with agricultural, pharmacological or nutritional interest, namely, for improving the coloring in strawberry and raspberry fruits, apart from improving the properties of raspberry and strawberry extracts for the inhibitory effect on alpha-glucosidase, ACE and COX2 for improving symptoms or preventing the metabolic syndrome.
- QV15 laboratory internal code
- Bacillus amyloliquefaciens QV15 is able to modulate the secondary metabolism of plants, as well as to improve the properties of raspberry and strawberry fruit extracts, improving the inhibitory effect of alpha glucosidase, ACE and COX2 with respect to non-inoculated controls and, therefore, this bacterium can be used in any type of culture of agronomic, pharmacological or nutritional, agricultural or forestry interest, for the improvement of symptoms or prevention of metabolic syndrome.
- Taxonomic characterization of Bacillus amyloliquefaciens was performed by identifying the strain by 16S ribosomal DNA partial sequencing, its comparison with the sequences in the databases revealed a 100% homology with a Bacillus amyloliquefaciens strain.
- Photosynthesis was determined by fluorescence(Fo, Fv/Fm, ⁇ PSII, y NPQ), bioactive compounds (phenols, flavonones and anthocyanins) in leaves and fruits, nutritional values in fruits (pH, °Brix, and citric acid%); and finally, measurements have been made with the methanolic fruit extracts on the inhibition of enzymes related to glucose regulation (alpha amylase and alpha glucosidase), hypertension (ACE) and on inflammation (COX2), enzymes related to the metabolic syndrome.
- the raspberry extract was prepared from fresh raspberries of the "Adelita" variety.
- the blackberries were first lyophilized, then extracted with 80% methanol, centrifuged and the organic fraction vacuum evaporated. The extract with 20% water was characterized; while for the extracts used for the rest of the measurements, an extraction with 80% methanol was performed. So, the measurements obtained for alpha-glucosidase, ACE, and COX2 would be in dry weight while for the rest in fresh weight.
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
- Dentistry (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Cultivation Of Plants (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Claims (8)
- Bacillus amyloliquefaciens QV15 déposé sous le numéro CECT 9371, micro-organisme du groupe des bactéries Gram +, du genre Bacillus, caractérisé par sa capacité à stimuler le métabolisme secondaire des composés phénoliques des espèces végétales, à savoir les flavonoïdes, les anthocyanes et les catéchines, et par sa capacité à augmenter les degrés Brix.
- Bacillus amyloliquefaciens QV15 déposé sous le numéro CECT 9371, selon la revendication 1, caractérisé par sa capacité à augmenter la teneur en anthocyanes des fruits de fraises et de framboises.
- Bacillus amyloliquefaciens QV15 déposé sous le numéro CECT 9371, micro-organisme du groupe des bactéries Gram +, genre Bacillus, caractérisé par sa capacité à renforcer les propriétés des extraits de framboises et de fraises en tant qu'inhibiteurs d'enzymes liées au syndrome métabolique : alpha-glucosidase, régulateurs de la glycémie, ACE, convertisseur d'angiotensine pour l'hypertension et COX2, inflammation.
- Utilisation de Bacillus amyloliquefaciens QV15 déposé sous le numéro CECT 9371, selon les revendications 1 à 3, pour application à tout type de culture d'intérêt agricole, pharmacologique ou nutritionnel, dans le secteur agricole ou forestier, afin d'augmenter les bioactifs et/ou d'améliorer les extraits de feuilles et/ou de fruits pour leur effet sur l'alpha-glucosidase, l'ECA et la COX2.
- Utilisation de Bacillus amyloliquefaciens QV15 déposé sous le numéro CECT 9371, selon les revendications 1 à 3, pour application à toute espèce végétale appartenant à la famille des Rubus sp.
- Utilisation de Bacillus amyloliquefaciens QV15 déposé sous CECT 9371, selon les revendications 1 à 3, pour application à toute espèce végétale dont font partie les baies rouges ou fruits sauvages (tels que fraise, framboise, mûre, myrtille ou myrtille) ou ceux issus du raisin, dans le but d'améliorer les qualités organoleptiques et la coloration des fruits, en particulier, à partir des anthocyanes.
- Utilisation de Bacillus amyloliquefaciens QV15 déposé comme CECT 9371, selon les revendications 1 à 3, soit la souche individuelle ou en combinaison avec d'autres organismes, faisant partie de toute préparation, soit individuellement ou en combinaison avec d'autres organismes, et par tout moyen disponible dans lequel la bactérie entre en contact avec la graine, la racine ou le système aérien des plantes.
- Utilisation de Bacillus amyloliquefaciens QV15 déposé sous le numéro CECT 9371, selon les revendications 1 à 3, soit la souche individuelle, soit en combinaison avec d'autres organismes, ou faisant partie de toute préparation, soit individuellement, soit en combinaison avec d'autres organismes, et par tout moyen disponible dans lequel la bactérie entre en contact avec les cellules végétales dans n'importe quel état de différenciation sur une culture in vitro.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES201730818A ES2694593B2 (es) | 2017-06-21 | 2017-06-21 | Bacillus amyloliquefaciens QV15 estimulante del metabolismo secundario de compuestos fenólicos y de la capacidad inhibidora de los extractos de frambruesa y fresa sobre los enzimas relacionados con el síndrome metabólico |
| PCT/ES2018/070369 WO2018234599A1 (fr) | 2017-06-21 | 2018-05-22 | Bacillus amyloliquefaciens qv15 stimulant du métabolisme secondaire de composés phénoliques et de la capacité inhibitrice des extraits de framboise et de fraise sur les enzymes associés au syndrome métabolique |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| EP3643775A1 EP3643775A1 (fr) | 2020-04-29 |
| EP3643775A4 EP3643775A4 (fr) | 2021-03-31 |
| EP3643775B1 true EP3643775B1 (fr) | 2024-07-03 |
Family
ID=64735531
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP18820445.7A Active EP3643775B1 (fr) | 2017-06-21 | 2018-05-22 | Bacillus amyloliquefaciens qv15 stimulant du métabolisme secondaire de composés phénoliques et de la capacité inhibitrice des extraits de framboise et de fraise sur les enzymes associés au syndrome métabolique |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US20210076684A1 (fr) |
| EP (1) | EP3643775B1 (fr) |
| JP (1) | JP7122765B2 (fr) |
| AU (1) | AU2018287094B2 (fr) |
| BR (1) | BR112019026342A2 (fr) |
| CA (1) | CA3067875A1 (fr) |
| CL (1) | CL2019003465A1 (fr) |
| CO (1) | CO2019012690A2 (fr) |
| EA (1) | EA201992374A1 (fr) |
| ES (1) | ES2694593B2 (fr) |
| MA (1) | MA47663B1 (fr) |
| MX (1) | MX2019015261A (fr) |
| PE (1) | PE20200714A1 (fr) |
| TN (1) | TN2019000304A1 (fr) |
| WO (1) | WO2018234599A1 (fr) |
| ZA (1) | ZA202000371B (fr) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2902976B2 (es) * | 2021-09-21 | 2022-09-29 | Biobab R&D S L | Pseudomonas atacamensis CECT 30419 mejoradora de la producción vegetal y estimulante del metabolismo secundario de plantas. |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2336758B1 (es) | 2009-12-03 | 2011-04-06 | Fundacion Universitaria San Pablo Ceu | Pseudomonas fluorescens n. 21.4 estimulante del metabolismo secundario de compuestos fenolicos. |
| KR20110120748A (ko) * | 2010-04-29 | 2011-11-04 | 충남대학교산학협력단 | 신규한 미생물 바실러스 아밀로리큐파션스 씨피 1 및 이를 이용한 딸기 탄저병균의 방제방법 |
| CN102876603B (zh) * | 2012-09-23 | 2013-11-13 | 江苏丘陵地区镇江农业科学研究所 | 一种解淀粉芽孢杆菌Mg116菌株及用途 |
| JP2015181423A (ja) * | 2014-03-25 | 2015-10-22 | 株式会社アイエイアイ | バチルス属に属する菌株、微生物製剤、及び植物の栽培方法 |
| AR103288A1 (es) * | 2014-12-29 | 2017-04-26 | Fmc Corp | Composiciones de bacillus amyloliquefaciens rti472 y métodos de uso para beneficiar el crecimiento de las plantas y el tratamiento de enfermedades de las plantas |
-
2017
- 2017-06-21 ES ES201730818A patent/ES2694593B2/es active Active
-
2018
- 2018-05-22 MA MA47663A patent/MA47663B1/fr unknown
- 2018-05-22 JP JP2019571539A patent/JP7122765B2/ja active Active
- 2018-05-22 US US16/602,882 patent/US20210076684A1/en not_active Abandoned
- 2018-05-22 CA CA3067875A patent/CA3067875A1/fr active Pending
- 2018-05-22 WO PCT/ES2018/070369 patent/WO2018234599A1/fr unknown
- 2018-05-22 TN TNP/2019/000304A patent/TN2019000304A1/en unknown
- 2018-05-22 BR BR112019026342-0A patent/BR112019026342A2/pt unknown
- 2018-05-22 EP EP18820445.7A patent/EP3643775B1/fr active Active
- 2018-05-22 AU AU2018287094A patent/AU2018287094B2/en active Active
- 2018-05-22 PE PE2019002272A patent/PE20200714A1/es unknown
- 2018-05-22 MX MX2019015261A patent/MX2019015261A/es unknown
- 2018-05-22 EA EA201992374A patent/EA201992374A1/ru unknown
-
2019
- 2019-11-14 CO CONC2019/0012690A patent/CO2019012690A2/es unknown
- 2019-11-27 CL CL2019003465A patent/CL2019003465A1/es unknown
-
2020
- 2020-01-20 ZA ZA2020/00371A patent/ZA202000371B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| MX2019015261A (es) | 2020-02-12 |
| ZA202000371B (en) | 2021-05-26 |
| AU2018287094B2 (en) | 2024-07-11 |
| WO2018234599A1 (fr) | 2018-12-27 |
| CA3067875A1 (fr) | 2018-12-27 |
| CL2019003465A1 (es) | 2020-05-29 |
| BR112019026342A2 (pt) | 2020-07-21 |
| MA47663B1 (fr) | 2020-10-28 |
| CO2019012690A2 (es) | 2020-01-17 |
| US20210076684A1 (en) | 2021-03-18 |
| ES2694593B2 (es) | 2019-06-28 |
| EP3643775A4 (fr) | 2021-03-31 |
| EP3643775A1 (fr) | 2020-04-29 |
| EA201992374A1 (ru) | 2020-05-28 |
| JP2020524522A (ja) | 2020-08-20 |
| ES2694593A1 (es) | 2018-12-21 |
| MA47663A1 (fr) | 2020-05-29 |
| TN2019000304A1 (en) | 2021-05-07 |
| PE20200714A1 (es) | 2020-06-30 |
| JP7122765B2 (ja) | 2022-08-22 |
| AU2018287094A1 (en) | 2020-02-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Haidar et al. | Screening and modes of action of antagonistic bacteria to control the fungal pathogen Phaeomoniella chlamydospora involved in grapevine trunk diseases | |
| Fu et al. | Plant growth-promoting traits of yeasts isolated from the phyllosphere and rhizosphere of Drosera spatulata Lab. | |
| Apaliya et al. | Hanseniaspora uvarum enhanced with trehalose induced defense-related enzyme activities and relative genes expression levels against Aspergillus tubingensis in table grapes | |
| Sanzani et al. | Study on the role of patulin on pathogenicity and virulence of Penicillium expansum | |
| Wang et al. | Study on biocontrol of postharvest decay of table grapes caused by Penicillium rubens and the possible resistance mechanisms by Yarrowia lipolytica | |
| Hammami et al. | Isolation and characterization of rhizosphere bacteria for the biocontrol of the damping-off disease of tomatoes in Tunisia | |
| Arfaoui et al. | Isolation, identification and in vitro characterization of grapevine rhizobacteria to control ochratoxigenic Aspergillus spp. on grapes | |
| Kriaa et al. | Biocontrol of tomato plant diseases caused by Fusarium solani using a new isolated Aspergillus tubingensis CTM 507 glucose oxidase | |
| Lin et al. | Study on the biocontrol effect and physiological mechanism of Hannaella sinensis on the blue mold decay of apples | |
| Abdel-Kareem et al. | Novel marine yeast strains as plant growth-promoting agents improve defense in wheat (Triticum aestivum) against Fusarium oxysporum | |
| Li et al. | CsMYB60 confers enhanced resistance to fusarium solani by increasing Proanthocyanidin biosynthesis in cucumber | |
| EP3643775B1 (fr) | Bacillus amyloliquefaciens qv15 stimulant du métabolisme secondaire de composés phénoliques et de la capacité inhibitrice des extraits de framboise et de fraise sur les enzymes associés au syndrome métabolique | |
| Pal et al. | Seed endophytic bacterium Bacillus velezensis and its lipopeptides acts as elicitors of defense responses against Fusarium verticillioides in maize seedlings | |
| Shobha et al. | Induction of systemic resistance by rhizobacterial and endophytic fungi against foot rot disease of Piper nigrum L. by increasing enzyme defense activity | |
| KR100423121B1 (ko) | 길항미생물 바실러스 아밀로리퀴파시엔스 조104 및 이균주가 생산하는 항균물질 | |
| Tedsree et al. | Diversity and antimicrobial activity of plant growth promoting endophytic actinomycetes isolated from Thai orchids | |
| Alhasan et al. | In Vitro Antimicrobial Activity of The Filtrate Crude Extract Produced by Aspergillus niger | |
| Tlais et al. | Ecological linkages between biotechnologically relevant autochthonous microorganisms and phenolic compounds in sugar apple fruit (Annona squamosa L.) | |
| Patel et al. | Elicitation of plant defense enzymes against Fusarium oxysporum f. sp. lycopersici in tomato plant using a novel rhizobacteria Providencia rettgeri MSS2 | |
| Abied et al. | Alleviation of black root rot symptoms and alteration of strawberry growth via modulating physiological and biochemical mechanisms using Trichoderma viride and Bacillus subtilis | |
| Hasan et al. | Characterization and antagonistic control measures of Pichia kudriavzevii yeast like fungus in brown rot disease of Citrus sinensis (L.) Osbeck | |
| CN112410273A (zh) | 一种地衣芽孢杆菌w10菌悬液在诱导油桃果实抗褐腐病中的应用 | |
| Lekatompessy et al. | Potential of isolated bacteria biostimulant from soil roots on white turmeric plants (Curcuma zedoaria) | |
| Hamzah et al. | Act of malondialdehyde and total phenolic content under bean yellow mosaic virus infection and biostimulants application | |
| EP4421472A1 (fr) | Pseudomonas atacamensis cect 30419 améliorant la production végétale et stimulant le métabolisme secondaire de plantes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE |
|
| PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE |
|
| 17P | Request for examination filed |
Effective date: 20200110 |
|
| AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR |
|
| AX | Request for extension of the european patent |
Extension state: BA ME |
|
| DAV | Request for validation of the european patent (deleted) | ||
| DAX | Request for extension of the european patent (deleted) | ||
| A4 | Supplementary search report drawn up and despatched |
Effective date: 20210226 |
|
| RIC1 | Information provided on ipc code assigned before grant |
Ipc: A01N 63/22 20200101ALI20210222BHEP Ipc: C12R 1/07 20060101ALI20210222BHEP Ipc: C05F 11/08 20060101ALI20210222BHEP Ipc: C12N 1/20 20060101AFI20210222BHEP |
|
| GRAP | Despatch of communication of intention to grant a patent |
Free format text: ORIGINAL CODE: EPIDOSNIGR1 |
|
| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: GRANT OF PATENT IS INTENDED |
|
| INTG | Intention to grant announced |
Effective date: 20240326 |
|
| GRAS | Grant fee paid |
Free format text: ORIGINAL CODE: EPIDOSNIGR3 |
|
| GRAA | (expected) grant |
Free format text: ORIGINAL CODE: 0009210 |
|
| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE PATENT HAS BEEN GRANTED |
|
| AK | Designated contracting states |
Kind code of ref document: B1 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR |
|
| REG | Reference to a national code |
Ref country code: CH Ref legal event code: EP |
|
| REG | Reference to a national code |
Ref country code: DE Ref legal event code: R096 Ref document number: 602018071373 Country of ref document: DE |
|
| REG | Reference to a national code |
Ref country code: NL Ref legal event code: FP |