EP3569705A4 - Méthode d'expression efficace de pullulanase dans bacillus subtilis et bacillus subtilis recombinant - Google Patents

Méthode d'expression efficace de pullulanase dans bacillus subtilis et bacillus subtilis recombinant Download PDF

Info

Publication number
EP3569705A4
EP3569705A4 EP17891647.4A EP17891647A EP3569705A4 EP 3569705 A4 EP3569705 A4 EP 3569705A4 EP 17891647 A EP17891647 A EP 17891647A EP 3569705 A4 EP3569705 A4 EP 3569705A4
Authority
EP
European Patent Office
Prior art keywords
bacillus subtilis
efficiently expressing
recombinant
expressing pullulanase
pullulanase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP17891647.4A
Other languages
German (de)
English (en)
Other versions
EP3569705A1 (fr
Inventor
Yangyuan LI
Jianrong Wang
Ling Yang
Man QU
Lizhi Chen
Jiale Huang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangdong Vtr Bio-Tech Co Ltd
Original Assignee
Guangdong Vtr Bio-Tech Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangdong Vtr Bio-Tech Co Ltd filed Critical Guangdong Vtr Bio-Tech Co Ltd
Publication of EP3569705A1 publication Critical patent/EP3569705A1/fr
Publication of EP3569705A4 publication Critical patent/EP3569705A4/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP17891647.4A 2017-01-16 2017-10-24 Méthode d'expression efficace de pullulanase dans bacillus subtilis et bacillus subtilis recombinant Pending EP3569705A4 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201710032183.9A CN106754833B (zh) 2017-01-16 2017-01-16 在枯草芽孢杆菌中高效表达普鲁兰酶的方法及重组枯草芽孢杆菌
PCT/CN2017/107476 WO2018129980A1 (fr) 2017-01-16 2017-10-24 Méthode d'expression efficace de pullulanase dans bacillus subtilis et bacillus subtilis recombinant

Publications (2)

Publication Number Publication Date
EP3569705A1 EP3569705A1 (fr) 2019-11-20
EP3569705A4 true EP3569705A4 (fr) 2020-12-16

Family

ID=58946008

Family Applications (1)

Application Number Title Priority Date Filing Date
EP17891647.4A Pending EP3569705A4 (fr) 2017-01-16 2017-10-24 Méthode d'expression efficace de pullulanase dans bacillus subtilis et bacillus subtilis recombinant

Country Status (4)

Country Link
US (1) US10975366B2 (fr)
EP (1) EP3569705A4 (fr)
CN (1) CN106754833B (fr)
WO (1) WO2018129980A1 (fr)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106754833B (zh) * 2017-01-16 2020-06-09 广东溢多利生物科技股份有限公司 在枯草芽孢杆菌中高效表达普鲁兰酶的方法及重组枯草芽孢杆菌
CN107058367A (zh) * 2017-06-03 2017-08-18 白银赛诺生物科技有限公司 高产普鲁兰酶的重组枯草芽孢杆菌的构建方法
CN108330079A (zh) * 2017-10-25 2018-07-27 南京福斯弗瑞生物科技有限公司 一种产普鲁兰酶的解淀粉芽孢杆菌、其发酵得到的普鲁兰酶及重组载体的构建与应用
CN108570477B (zh) * 2018-04-18 2020-06-30 横琴仲泰生物医药有限公司 一种碱性蛋白酶基因及其重组枯草芽孢杆菌菌株的构建方法
CN108998403B (zh) * 2018-09-19 2021-12-07 长春大学 一种枯草芽孢杆菌重组菌及其应用
CN110106128B (zh) * 2019-04-24 2021-08-17 天津科技大学 一种生产重组碱性蛋白酶的基因工程菌及其构建方法
CN110373403B (zh) * 2019-08-19 2021-08-10 白银赛诺生物科技有限公司 耐高温中性普鲁兰酶及其应用
CN112779178B (zh) * 2019-11-08 2023-02-17 财团法人农业科技研究院 地衣芽孢杆菌a6菌株及其应用
CN113801830B (zh) * 2020-06-12 2023-05-26 青岛蔚蓝生物股份有限公司 一种高产普鲁兰酶的枯草芽孢杆菌菌株及其应用
CN111944790B (zh) * 2020-07-01 2022-09-09 深圳润康生态环境股份有限公司 中性蛋白酶基因、中性蛋白酶及其制备方法和应用
CN111893126A (zh) * 2020-07-01 2020-11-06 深圳润康生态环境股份有限公司 碱性蛋白酶基因、碱性蛋白酶及其制备方法和应用
CN111808834B (zh) * 2020-07-12 2023-08-04 广东溢多利生物科技股份有限公司 在枯草芽孢杆菌中高效表达耐高温α-淀粉酶的方法、重组启动子以及应用
CN111850096B (zh) * 2020-07-29 2022-02-01 江南大学 一种基于n端编码序列改造调控蛋白质表达的方法
CN111808177B (zh) * 2020-07-30 2022-06-21 吉林中粮生化有限公司 提高蛋白表达量的信号肽及其应用
CN111850008B (zh) * 2020-07-30 2023-10-20 吉林中粮生化有限公司 促进蛋白胞外表达的信号肽
CN111826377B (zh) * 2020-07-30 2023-09-01 吉林中粮生化有限公司 促进普鲁兰酶胞外表达的信号肽
CN111996205A (zh) * 2020-08-10 2020-11-27 深圳润康生态环境股份有限公司 几丁质酶基因、几丁质酶及其制备方法和应用
CN113667662A (zh) * 2021-07-05 2021-11-19 南京纽田科技有限公司 高性能淀粉脱支酶嵌合体菌种构建及其生产方法和应用
CN113584057B (zh) * 2021-07-26 2023-08-15 天津大学 Iccg表达元件及表达载体、枯草芽孢杆菌重组菌株和降解pet或其单体的方法
CN114574514B (zh) * 2022-03-30 2022-11-18 威海迪普森生物科技有限公司 纳豆激酶重组表达载体、重组基因工程菌及应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5264366A (en) * 1984-05-29 1993-11-23 Genencor, Inc. Protease deficient bacillus
WO2009058518A1 (fr) * 2007-10-31 2009-05-07 Danisco Us Inc., Genencor Division Utilisation et production de métalloprotéases neutres dans un milieu exempt de sérine protéase
CN104073458A (zh) * 2013-03-26 2014-10-01 南京金斯瑞生物科技有限公司 一株可高效表达外源分泌蛋白酶的枯草芽孢杆菌
WO2016134213A2 (fr) * 2015-02-19 2016-08-25 Danisco Us Inc Expression améliorée de protéine

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5294542A (en) * 1991-03-19 1994-03-15 Omnigene, Inc. Residual protease-III
JP3529774B2 (ja) * 1991-11-14 2004-05-24 ノボザイムス アクティーゼルスカブ バシラス・リヘニフォルミスｘ−アミラーゼのプロモーターの変異体由来のバシラス属細菌のプロモーター
US5955310A (en) * 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
US6287826B1 (en) * 1998-03-09 2001-09-11 Novo Nordisk A/S Enzymatic preparation of glucose syrup from starch
CN103224949B (zh) * 2013-05-13 2014-08-20 南宁邦尔克生物技术有限责任公司 一种高效表达重组普鲁兰酶的枯草芽孢杆菌及其获得方法
WO2015021601A1 (fr) * 2013-08-13 2015-02-19 Danisco Us Inc. Liquéfaction et malto-saccharification simultanées
DK3102669T3 (da) * 2014-02-07 2019-08-19 Dsm Ip Assets Bv Forbedret bacillevært
CN107002110A (zh) * 2014-10-10 2017-08-01 恩细贝普有限公司 用具有改善的合成水解比的枯草杆菌蛋白酶变体的肽片段缩合和环化
EP3253869A4 (fr) * 2015-02-04 2018-11-07 Nanjing Bestzyme Bio-Engineering Co. Ltd. Pullulanases tronquées, procédés de production et procédés d'utilisation de ces dernières
CN106754833B (zh) * 2017-01-16 2020-06-09 广东溢多利生物科技股份有限公司 在枯草芽孢杆菌中高效表达普鲁兰酶的方法及重组枯草芽孢杆菌

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5264366A (en) * 1984-05-29 1993-11-23 Genencor, Inc. Protease deficient bacillus
WO2009058518A1 (fr) * 2007-10-31 2009-05-07 Danisco Us Inc., Genencor Division Utilisation et production de métalloprotéases neutres dans un milieu exempt de sérine protéase
CN104073458A (zh) * 2013-03-26 2014-10-01 南京金斯瑞生物科技有限公司 一株可高效表达外源分泌蛋白酶的枯草芽孢杆菌
WO2016134213A2 (fr) * 2015-02-19 2016-08-25 Danisco Us Inc Expression améliorée de protéine

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2018129980A1 *

Also Published As

Publication number Publication date
CN106754833B (zh) 2020-06-09
WO2018129980A1 (fr) 2018-07-19
US20200140839A1 (en) 2020-05-07
CN106754833A (zh) 2017-05-31
US10975366B2 (en) 2021-04-13
EP3569705A1 (fr) 2019-11-20

Similar Documents

Publication Publication Date Title
EP3569705A4 (fr) Méthode d'expression efficace de pullulanase dans bacillus subtilis et bacillus subtilis recombinant
EP3682746A4 (fr) Agent anti-âge et procédé anti-âge
EP3710002A4 (fr) Agents de dégradation et dégrons pour dégradation protéique ciblée
EP3664508A4 (fr) Procédé de commutation d'instances et dispositif associé
EP3635760A4 (fr) Procédé et système pour une distribution d'énergie sans fil
EP3600178A4 (fr) Procédés et dispositifs de réduction de pression
EP3669605A4 (fr) Procédé et appareil de communication en liaison latérale
EP3146753A4 (fr) Système et procédé de commercialisation de services de publicité mobile
EP3550011A4 (fr) Bacillus subtilis, son procédé de culture et application associée
EP3589065A4 (fr) Procédé et appareil de commutation
EP3576245A4 (fr) Système d'alimentation électrique et procédé d'alimentation électrique
EP3493244A4 (fr) Tampon de polissage et procédé de polissage l'utilisant
EP3614707A4 (fr) Procédé et appareil de commutation d'état
EP3850954A4 (fr) Agent anti-âge et procédé anti-âge
EP3304470A4 (fr) Système et procédé d'intégration de fidélité pour portefeuilles numériques spécifiques de marchand
EP3582252A4 (fr) Liquide de polissage et procédé de polissage
EP3437798A4 (fr) Tampon de polissage et procédé de polissage
EP3697147A4 (fr) Procédé de configuration de service et produit associé
EP3572507A4 (fr) Procédé d'expression pour protéine recombinante d'haemocoagulase acutus
EP3477574A4 (fr) Système de fourniture de publicité mobile et procédé
EP3670238A4 (fr) Système d'alimentation électrique et son procédé de fonctionnement
EP3609739A4 (fr) Procédé d'échange et agencement pour l'échange d'unités de chargement
EP3605589A4 (fr) Composition de polissage et procédé de polissage
EP3326751A4 (fr) Tampon de polissage et procédé de polissage
EP3525147A4 (fr) Procédé et dispositif de partage de message dans une région

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE

17P Request for examination filed

Effective date: 20190813

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

AX Request for extension of the european patent

Extension state: BA ME

DAV Request for validation of the european patent (deleted)
DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20201113

RIC1 Information provided on ipc code assigned before grant

Ipc: C12N 9/44 20060101AFI20201109BHEP

Ipc: C12N 1/21 20060101ALI20201109BHEP

Ipc: C12R 1/125 20060101ALI20201109BHEP