EP3554700C0 - Thermalisierender mikrofluidischer chip mit variablen temperaturzyklen, system mit solch einem chip und pcr-verfahren zum nachweis von dna-sequenzen - Google Patents

Thermalisierender mikrofluidischer chip mit variablen temperaturzyklen, system mit solch einem chip und pcr-verfahren zum nachweis von dna-sequenzen

Info

Publication number
EP3554700C0
EP3554700C0 EP17822628.8A EP17822628A EP3554700C0 EP 3554700 C0 EP3554700 C0 EP 3554700C0 EP 17822628 A EP17822628 A EP 17822628A EP 3554700 C0 EP3554700 C0 EP 3554700C0
Authority
EP
European Patent Office
Prior art keywords
chip
thermalizing
detection
dna sequences
pcr method
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP17822628.8A
Other languages
English (en)
French (fr)
Other versions
EP3554700A1 (de
EP3554700B1 (de
Inventor
Maël Leberre
Adrien Plessis
Walter Minnella
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bforcure
Original Assignee
Bforcure
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR1601823A external-priority patent/FR3060418B1/fr
Priority claimed from FR1762058A external-priority patent/FR3074810B1/fr
Application filed by Bforcure filed Critical Bforcure
Publication of EP3554700A1 publication Critical patent/EP3554700A1/de
Application granted granted Critical
Publication of EP3554700B1 publication Critical patent/EP3554700B1/de
Publication of EP3554700C0 publication Critical patent/EP3554700C0/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F28—HEAT EXCHANGE IN GENERAL
    • F28F—DETAILS OF HEAT-EXCHANGE AND HEAT-TRANSFER APPARATUS, OF GENERAL APPLICATION
    • F28F3/00—Plate-like or laminated elements; Assemblies of plate-like or laminated elements
    • F28F3/12—Elements constructed in the shape of a hollow panel, e.g. with channels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B01L2300/185—Means for temperature control using fluid heat transfer medium using a liquid as fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0655—Valves, specific forms thereof with moving parts pinch valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F28—HEAT EXCHANGE IN GENERAL
    • F28F—DETAILS OF HEAT-EXCHANGE AND HEAT-TRANSFER APPARATUS, OF GENERAL APPLICATION
    • F28F2260/00—Heat exchangers or heat exchange elements having special size, e.g. microstructures
    • F28F2260/02—Heat exchangers or heat exchange elements having special size, e.g. microstructures having microchannels

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Biochemistry (AREA)
  • Mechanical Engineering (AREA)
  • General Engineering & Computer Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
EP17822628.8A 2016-12-19 2017-12-14 Thermalisierender mikrofluidischer chip mit variablen temperaturzyklen, system mit solch einem chip und pcr-verfahren zum nachweis von dna-sequenzen Active EP3554700B1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR1601823A FR3060418B1 (fr) 2016-12-19 2016-12-19 Puce micro fluidique, systeme utilisant une telle puce et procede pcr pour la detection de sequences adn
FR1762058A FR3074810B1 (fr) 2017-12-13 2017-12-13 Puce echantillon micro-fluidique, systeme d'analyse utilisant une telle puce et procede pcr pour la detection de sequences adn
PCT/EP2017/082898 WO2018114620A1 (fr) 2016-12-19 2017-12-14 Puce micro fluidique de thermalisation à cycles de température variable, système utilisant une telle puce et procédé pcr pour la détection de séquences adn

Publications (3)

Publication Number Publication Date
EP3554700A1 EP3554700A1 (de) 2019-10-23
EP3554700B1 EP3554700B1 (de) 2024-02-21
EP3554700C0 true EP3554700C0 (de) 2024-02-21

Family

ID=60888397

Family Applications (2)

Application Number Title Priority Date Filing Date
EP17822629.6A Pending EP3554701A1 (de) 2016-12-19 2017-12-14 Mikrofluidische probenchip, testsystem mit verwendung solch eines chips und pcr-verfahren zur detektion von dna-sequenzen
EP17822628.8A Active EP3554700B1 (de) 2016-12-19 2017-12-14 Thermalisierender mikrofluidischer chip mit variablen temperaturzyklen, system mit solch einem chip und pcr-verfahren zum nachweis von dna-sequenzen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
EP17822629.6A Pending EP3554701A1 (de) 2016-12-19 2017-12-14 Mikrofluidische probenchip, testsystem mit verwendung solch eines chips und pcr-verfahren zur detektion von dna-sequenzen

Country Status (4)

Country Link
US (3) US11198120B2 (de)
EP (2) EP3554701A1 (de)
CN (2) CN110191758B (de)
WO (2) WO2018114625A1 (de)

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EP3743524A4 (de) * 2018-01-22 2021-10-27 University of Washington Verfahren zur durchführung einer digitalen nukleinsäureamplifikation unter verwendung von polybuten
IT201800006089A1 (it) * 2018-06-06 2019-12-06 Dispositivo microfluidico per la concentrazione di particelle
CN110554651B (zh) * 2019-09-19 2021-07-30 哈尔滨工业大学 微流控芯片温度测量及控制的私有物联网系统
CN110756236B (zh) * 2019-11-18 2021-12-17 江苏纳迪芯生命科技研究院有限公司 一种密封式微流控乳化芯片及其制作工艺和使用方法
EP3831490A1 (de) * 2019-12-03 2021-06-09 Eppendorf AG Thermoblock zur aufnahme und temperierung mindestens eines laborprobengefässes, herstellungsverfahren und simulationsverfahren
EP3838411A1 (de) * 2019-12-18 2021-06-23 TECAN Trading AG Pipettiervorrichtung und -verfahren
CN111185248A (zh) * 2020-01-19 2020-05-22 中国科学院上海微系统与信息技术研究所 一种含有气泡消除结构的聚合酶链式反应芯片及液滴乳液的处理方法
US20230234050A1 (en) * 2020-05-28 2023-07-27 National University Of Singapore Microfluidic chip and system
FR3117045A1 (fr) 2020-12-04 2022-06-10 Bforcure Puce de détection PCR, appareil de test associé et système d’analyse de mise en œuvre
CN114618599A (zh) * 2020-12-14 2022-06-14 京东方科技集团股份有限公司 加热控温装置及微流控系统
CN112684320B (zh) * 2020-12-17 2023-01-31 海光信息技术股份有限公司 芯片低温测试环境仓及芯片测试机
CN113720834B (zh) * 2021-08-25 2023-08-18 中国科学院南海海洋研究所 一种用于水体生化要素检测的微流控芯片、系统及方法
FR3136386A1 (fr) 2022-06-08 2023-12-15 Bforcure Puce de détection PCR, appareil de test associé et système d’analyse de mise en œuvre
CN114923354B (zh) * 2022-06-09 2025-03-28 马虹霞 一种热传导转接模块及其应用
CN115651824A (zh) * 2022-06-27 2023-01-31 杭州聚致生物科技有限公司 Pcr反应系统和方法

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US5637469A (en) * 1992-05-01 1997-06-10 Trustees Of The University Of Pennsylvania Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems
US5508197A (en) 1994-07-25 1996-04-16 The Regents, University Of California High-speed thermal cycling system and method of use
US5958349A (en) 1997-02-28 1999-09-28 Cepheid Reaction vessel for heat-exchanging chemical processes
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
JP5046412B2 (ja) 2000-04-06 2012-10-10 カリパー・ライフ・サイエンシズ・インク． 被覆層を組み込んだミクロ流体装置及びシステム
US7440684B2 (en) 2001-04-12 2008-10-21 Spaid Michael A Method and apparatus for improved temperature control in microfluidic devices
TWI273240B (en) * 2004-09-27 2007-02-11 Univ Nat Cheng Kung Reverse transcription polymerase chain reaction chip
KR100773552B1 (ko) * 2006-04-28 2007-11-07 삼성전자주식회사 미세유체 반응 용기, 이의 제조 방법 및, 이를 이용한미세유체 반응 방법
WO2009029845A1 (en) 2007-08-29 2009-03-05 Plexera Bioscience Llc Microfluidic apparatus for wide area microarrays
US9034635B2 (en) 2008-02-20 2015-05-19 Streck, Inc. Thermocycler and sample vessel for rapid amplification of DNA
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Also Published As

Publication number Publication date
US20200016593A1 (en) 2020-01-16
CN110191759B (zh) 2022-10-11
WO2018114620A1 (fr) 2018-06-28
US20190388887A1 (en) 2019-12-26
CN110191758A (zh) 2019-08-30
US20220097049A1 (en) 2022-03-31
CN110191759A (zh) 2019-08-30
CN110191758B (zh) 2022-10-14
EP3554701A1 (de) 2019-10-23
WO2018114625A1 (fr) 2018-06-28
EP3554700A1 (de) 2019-10-23
US11198120B2 (en) 2021-12-14
EP3554700B1 (de) 2024-02-21
US11607684B2 (en) 2023-03-21

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