EP2240600B1 - Mikrofluidvorrichtung für breitflächige mikroarrays - Google Patents

Mikrofluidvorrichtung für breitflächige mikroarrays Download PDF

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Publication number
EP2240600B1
EP2240600B1 EP08798995A EP08798995A EP2240600B1 EP 2240600 B1 EP2240600 B1 EP 2240600B1 EP 08798995 A EP08798995 A EP 08798995A EP 08798995 A EP08798995 A EP 08798995A EP 2240600 B1 EP2240600 B1 EP 2240600B1
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Prior art keywords
fluid
flow chamber
analysis
microns
microfluidic device
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English (en)
French (fr)
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EP2240600A1 (de
EP2240600A4 (de
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Gibum Kim
R. Todd Schwoerer
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Plexera Bioscience LLC
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Plexera Bioscience LLC
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Priority claimed from US11/846,883 external-priority patent/US20090060786A1/en
Priority claimed from US11/846,908 external-priority patent/US7695976B2/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles or throttle valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0825—Test strips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502776—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for focusing or laminating flows

Definitions

  • Microfluidic flow cells that are used in, for example, microarray chemical analysis typically have small holes for fluid inlet and outlet.
  • the flow becomes localized between the fluid inlet and outlet, which results in high non-uniformity of the analyte across the surface of the microarray.
  • a higher flow rate for the analyte fluid may be used to exchange the fluid in the flow cell; however, such high flow rates are undesirable because the analyte is often precious and may be present at low concentration.
  • US patent application US2007140918 A 1 relates to fluidic separation devices and methods of using the same with reduced sample broadening.
  • the device contains a holding chamber, a separation column located downstream from the holding chamber, and means for providing flow effective to convey a sample along a flow path that extends from the holding chamber into the separation column.
  • One embodiment is a microfluidic device for contacting a surface with a fluid that comprises: a) a fluid inlet having a semi-circular groove; and b) a flow chamber that comprises the surface and an inner wall having an inlet end and an outlet end.
  • the fluid inlet is in communication with the flow chamber.
  • the inner wall is curved with a radius of curvature similar to the radius of curvature of the fluid inlet semi-circular groove, such that the fluid inlet is designed to introduce the fluid to the flow chamber through the inlet end.
  • the fluid enters the flow chamber and forms a pattern on the surface, the pattern approximating the semi-circular groove, and upon maintaining the flow a linear fluid front forms on the surface and moves along the surface.
  • a microfluidic device that comprises: a) a fluid inlet having a semi-circular groove and b) a flow chamber having an inlet end, wherein the fluid inlet and flow chamber are in communication and wherein the inner wall at the inlet end is curved with a radius similar to the radius of the semi-circular groove.
  • An analyte fluid introduced through the groove flows across the surface of a microarray with high uniformity and does not require high analyte fluid volumes to exchange the fluid in the flow cell.
  • the flow chamber may be in communication with a sample chip.
  • fluid flows through the flow chamber and is contained between a) the bottom surface adjacent to the fluid inlet and b) the surface of the sample chip.
  • the surface of the sample chip may have an analysis area to which is immobilized, for example, probe molecules such as peptides, proteins, DNA, RNA, etc.
  • the microfluidic device further comprises a fluid outlet end having a fluid outlet, wherein the inner wall of the flow chamber at the outlet end tapers toward the fluid outlet.
  • Another embodiment is an assembly for chemical analysis comprising any of the microfluidic devices as described above and the sample chip having a surface comprising an analysis area, wherein the surface is in communication with the flow chamber.
  • Another embodiment is a method of chemical analysis comprising: a) introducing an analyte fluid having a flow to a surface of a sample chip through a microfluidic device comprising i) a fluid inlet having a semi-circular groove and ii) a flow chamber comprising the surface and an inner wall having an inlet end and an outlet end, wherein the fluid inlet is in communication with the flow chamber, wherein the inner wall at the inlet end is curved with a radius of curvature similar to the radius of curvature of the fluid inlet semi-circular groove, wherein the fluid inlet introduces the fluid to the flow chamber through the inlet end, wherein the fluid enters the flow chamber and forms a fluid front as the fluid contacts the surface; b) maintaining the flow of the analyte fluid such that the analyte fluid forms a pattern on the surface of the sample chip, the pattern approximating the semi-circular groove; c) maintaining the flow of the analyte fluid so that a linear fluid
  • a microfluidic device that comprises: a) a fluid inlet 10 having a semi-circular groove and b) a flow chamber 20 comprising an inner wall 25 having an inlet end 30, wherein fluid inlet 10 is in communication with flow chamber 20 and wherein inner wall 25 at inlet end 30 is curved with a radius similar to the radius of the semi-circular groove.
  • Flow chamber 20 may be in communication with a sample chip 35.
  • fluid flows through flow chamber 20 and is contained between bottom surface 40 and surface 45 of sample chip 35.
  • Surface 45 of sample chip 35 may have analysis area 50 to which is immobilized, for example, probe molecules, peptides, proteins, DNA, RNA, etc.
  • FIG 2a illustrates the microfluidic device from a "top down" view relative to FIG 1 .
  • FIG 2a shows fluid inlet 10 having a semi-circular groove, the outline of analysis area 50, the outline of inner wall 25, the radius 55 of the fluid inlet 10 having a semi-circular groove, and radius 60 of inner wall 25 at inlet end 30.
  • FIG 2b illustrates a cross section view of the microfluidic device along plane 65 ( FIG 2a).
  • FIG 2b shows the cross section of the fluid inlet 10 having a semi-circular groove, flow chamber 20, inner wall 25, sample chip 35, and space 70 where the a fluid in contained between bottom surface 40 and surface 45 of sample chip 35.
  • FIG 3 illustrates a method of operating the microfluidic device by introducing a fluid flow 75 at roughly the middle 80 of fluid inlet 10 having a semi-circular groove.
  • the fluid flow passes through flow chamber 20 at inlet end 30 and contacts surface 45 of sample chip 35. From the point of contact 85 with surface 45 of sample chip 35, the fluid flows outward 90a, 90b in a pattern that approximates semi-circular groove.
  • a linear front begins forming 95a at point of contact 85 and then flows 100 along surface 45 of sample chip 35 with the linear front 95b maintained.
  • the flow of fluid through flow chamber 20 is highly uniform across the relatively large surface area of a sample chip.
  • fluid inlet 10 is shown in a separate layer 5 and flow chamber 20 is shown in a separate layer 15.
  • fluid inlet 10 and flow chamber 20 need not be in separate layers.
  • fluid inlet 10 and flow chamber 20 comprise separate layers (e.g., layer 5 and layer 15, respectively). These layers can be, for example, pressure sensitive adhesive tape, or other material such as telfon, having a variety of thicknesses.
  • fluid inlet 10 and flow chamber 20 comprise a single layer and may be, for example, fabricated as one solid piece.
  • Such embodiments may have fluid inlet 10 and flow chamber 20 fabricated sequentially or concurrently by, for example, techniques that include machining of a solid block material, embossing a material, molded UV curing, molded thermosetting, etc. and any combination thereof.
  • Materials that the layers can be made from include plastics such as, for example Lucite or Teflon, metals and alloys, and glass or silicon.
  • Bottom surface 40, the inner walls of fluid inlet 10, and/or inner wall 25 of flow chamber 20 may have additional structures protruding into or receding from the fluid path in order to, for example, enhance mixing or improve general flow dynamics.
  • the microfluidic device may have one or more of the following features.
  • Radius 60 of inner wall 25 is from about 105% to about 107% larger than radius 55 of the semi-circular groove.
  • the width of the groove is from about 350 micron to about 500 micron and radius 55 of the semi-circular groove is from about 3.5 cm to about 4 cm.
  • the depth 71 of fluid inlet 10 is from about 25 microns to about 40 microns.
  • the depth 72 of flow chamber 20 is from about 13 microns to about 20 microns.
  • the microfluidic device further comprises a fluid outlet end 105 having a fluid outlet 110, wherein inner wall 25 of flow chamber 20 at outlet end 105 tapers (115) toward fluid outlet 110.
  • Apex 120 of the taper is allows fluid to flow through fluid outlet 110 and out of flow chamber 20.
  • the invention may include one or more of the following.
  • the length 125 of flow chamber 20 may be from about 3 cm to about 4.5 cm and the width 130 of flow chamber 20 may be about 1.4 cm to about 1.6 cm.
  • the volume of flow chamber 20 is from about 6 ⁇ L to about 10 ⁇ L.
  • the microfluidic device has a fluid exchange volume between about 80% and about 130% of the volume of flow chamber 20.
  • the depth 71 of fluid inlet 10 is about 13 microns to about 20 microns
  • semi-circular groove has a width of about 350 micron to about 500 micron and radius 55 of about 3.5 cm to about 4 cm
  • the depth 72 of flow chamber 20 is about 13 microns to about 20 microns
  • flow chamber 20 has length 125 of about 3 cm to 4.5 cm and width 130 of about 1.4 cm to about 1.6 cm.
  • Analysis area 50 is at least 1.5 sq. cm.
  • Analysis area 50 includes a microarray comprising analysis spots. At least one analysis spot of the microarray may comprise a biomolecule.
  • the biomolecule may be a polypeptide or a polynucleotide.
  • the microarray may also comprise a plurality of polypeptides, polynucleotides, or both.
  • FIGs 1-4 Another embodiment, referring to FIGs 1-4 , is a method of chemical analysis comprising: a) introducing an analyte fluid having a flow to surface 45 of sample chip 35 through a microfluidic device comprising i) a fluid inlet 10 having a semi-circular groove for fluid inlet and ii) a flow chamber 20 comprising an inner wall 25 having an inlet end 30, wherein the fluid inlet 10 is in communication with flow chamber 20 and wherein inner wall 25 at inlet end 30 is curved with a radius similar to the radius of the semi-circular groove.; b) maintaining the flow of the analyte fluid such that the analyte fluid forms a pattern on surface 45 of the sample chip 35, the pattern approximating the semi-circular groove; c) maintaining the flow of the analyte fluid so that linear fluid front 95a forms on surface 45 of sample chip 35 at inlet end 30; and d) maintaining the flow so that a linear fluid front 95b moves along surface 45 of sample chip
  • the various features of the microfluidic device may include those described above and illustrated in FIGs 1-4 . Embodiments may have one or more of the following features.
  • the flow has a rate of about 180 ⁇ L/min to about 600 ⁇ L/min and a pressure of about 5 to about 30 PSI (34,500 Pa to 206,800 Pa).
  • Surface 45 of sample chip 35 comprises analysis area 50 that is at least 1.5 sq. cm.
  • Analysis area 50 includes a microarray comprising analysis spots. At least one analysis spot comprises a biomolecule.
  • the biomolecule is a polypeptide or a polynucleotide.
  • the microarray comprises a plurality of polypeptides, polynucleotides, or both.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)
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Claims (15)

  1. Mikrofluidische Vorrichtung zum Kontaktieren einer Oberfläche mit einer Flüssigkeit, umfassend:
    a) einen Fluideinlass (10) mit einer halbkreisförmigen Rinne und
    b) eine Durchflusskammer (20) mit dieser Oberfläche und eine innere Wand (25) mit einem Einlassende (30) und ein Auslassende (105), wobei der Fluideinlass (10) in Verbindung steht mit der Durchflusskammer (20), dadurch gekennzeichnet, dass die Innenwand (25) am Einlassende (30) mit einem Krümmungsradius ähnlich dem Krümmungsradius des Fluideinlasses der halbkreisförmige Rinne gekrümmt ist, dass der Fluideinlass (10) derart gestaltet ist, Fluid zu der Strömungskammer (20) durch das Einlassende einzubringen, wodurch, bei Verwendung, die Flüssigkeit in die Durchflusskammer (20) eintritt und ein Muster auf der Oberfläche ausbildet, wobei das Muster sich der halbkreisförmigen Rinne annähert und wodurch nach Beibehalten des Flusses sich eine lineare Fluidfront (95b) auf der Oberfläche bildet und sich entlang der Oberfläche bewegt.
  2. Mikrofluidische Vorrichtung nach Anspruch 1, wobei:
    der Fluideinlass (10) und die Durchflusskammer (20) separate Schichten umfasst;
    der Fluideinlass (10) und die Durchflusskammer (20) eine einzelne Schicht umfasst;
    der Radius der inneren Wand etwa 5% bis etwa 7% größer ist als der Radius der Rinne;
    die Breite der Rinne etwa 350 Mikrometer bis etwa 500 Mikrometer und der Radius der Rinne etwa 1,75 cm bis etwa 2 cm beträgt;
    die Tiefe der Fluideinlass (10) etwa 25 Mikrometer bis etwa 40 Mikrometer beträgt, oder
    die Tiefe der Durchflusskammer (20) etwa 13 Mikrometer bis etwa 20 Mikrometer beträgt.
  3. Die Mikrofluidvorrichtung von Anspruch 1 oder 2, ferner umfassend ein Fluidauslassende (105) mit einem Fluidauslass (110), wobei die innere Wand (25) am Auslassende (105) sich hin zum Fluidauslass (110) verjüngt.
  4. Mikrofluidik-Vorrichtung nach einem der Ansprüche 1 bis 3, wobei die Länge des Durchflusskammer (20) etwa 3,5 cm bis etwa 4 cm beträgt und die Breite der Durchflusskammer etwa 1,4 cm bis etwa 1,6 cm beträgt.
  5. Mikrofluidik-Vorrichtung nach einem der Ansprüche 1 bis 4, wobei das Volumen der Durchflusskammer (20) etwa 6 µL bis etwa 10 µL beträgt.
  6. Mikrofluidik-Vorrichtung nach einem der Ansprüche 1 bis 5, wobei die mikrofluidische Vorrichtung ein Flüssigkeitsaustauschvolumen von etwa 80% bis etwa 130% des Volumens der Durchfluss besitzt.
  7. Mikrofluidik-Vorrichtung nach einem der Ansprüche 1 bis 6, wobei die Tiefe des Fluideinlasses (10) etwa 25 Mikrometer bis etwa 40 Mikrometer beträgt, die halbkreisförmige Rinne eine Breite von ungefähr 350 Mikrometer bis ungefähr 500 Mikrometer und einem Radius von etwa 1,8 cm bis etwa 2 cm besitzt, die Tiefe der Durchflusskammer (20) etwa 13 Mikron bis etwa 20 Mikron beträgt, und die Durchflusskammer (20) eine Länge von etwa 3 cm bis 4,5 cm und eine Breite von etwa 1,4 cm bis etwa 1,6 cm besitzt.
  8. Anordnung zur chemischen Analyse, umfassend die Mikrofluidik-Vorrichtung nach Anspruch 1 und einen Proben-Chip (35) mit einer Oberfläche (45) umfassend einen Analysebereich (50), wobei die Oberfläche (45) und die Durchflusskammer (20) in Verbindung stehen.
  9. Anordnung nach Anspruch 8, wobei:
    der Analysebereich (50) mindestens 1,5 cm2 beträgt;
    der Analysebereich (50) einen Mikroarray aufweist, der Analysepunkte umfasst;
    der Analysebereich (50) einen Mikroarray aufweist, der Analysepunkte umfasst und wenigstens ein Analysepunkte ein Biomolekül umfasst;
    der Analysebereich (50) einen Mikroarray aufweist, der Analysepunkte umfasst und
    wenigstens ein Analysepunkt ein Polypeptid oder ein Polynukleotid umfasst, oder
    der Mikroarray eine Vielzahl von Polypeptiden, Polynukleotiden oder beides umfasst.
  10. Verfahren zur chemischen Analyse, umfassend:
    a) Einführen eines Analytenfluids mit einem Fluss zu einer Oberfläche (45) eines Proben-Chips (35) durch eine mikrofluidische Vorrichtung, umfassend i) einen Fluideinlass (10) mit einer halbkreisförmigen Rinne und
    ii) eine Durchflusskammer (20) mit der Oberfläche und einer inneren Wand (25) mit einem Einlassende (30) und ein Auslassende (105), wobei der Fluideinlass (10) in Verbindung mit der Durchflusskammer (20) steht, wobei die innere Wand (25) am Einlassende (30) mit einem Krümmungsradius ähnlich dem Krümmungsradius des Fluideinlasses der halbkreisförmigen Rinne gekrümmt ist, wobei der Fluideinlass (10) das Fluid zur Durchflusskammer (20) durch das Einlassende (30) führt, wobei das Fluid in die Durchflusskammer (20) eintritt und ein Fluidfront (95a) ausbildet, wenn das Fluid in Kontakt mit der Oberfläche (45) kommt,
    b) Beibehalten des Flusses des Analytenfluids, so dass das Analytenfluid ein Muster auf der Oberfläche (45) des Proben-Chips (35) ausbildet, wobei das Muster sich der halbkreisförmigen Rinne annähert;
    c) Beibehalten des Flusses des Analytenfluids, so dass eine lineare Fluidfront (95b) sich auf der Oberfläche (45) des Proben-Chips (35) am Einlassende (30) ausbildet, und
    d) Beibehalten des Flusses, so dass die lineare Fluidfront (95b) sich entlang der Oberfläche (45) des Probe-Chip (35) bewegt.
  11. Verfahren zur chemischen Analyse nach Anspruch 10, wobei in der mikrofluidischen Vorrichtung:
    der Fluideinlass (10) und die Durchflusskammer (20) separate Schichten umfassen;
    der Fluideinlass (10) und die Durchflusskammer (20) eine einzelne Schicht umfassen;
    der Radius der inneren Wand (25) etwa 5% bis etwa 7% größer als ist der Radius der Rinne;
    die Breite der Rinne etwa 350 Mikrometer bis etwa 500 Mikrometer beträgt und der Radius der Rinne etwa 1,8 cm bis etwa 2 cm beträgt;
    die Tiefe der Fluideinlass etwa 20 Mikrometer bis etwa 40 Mikrometer beträgt, oder
    die Tiefe der Durchflusskammer etwa 13 Mikrometer bis etwa 20 Mikrometer beträgt.
  12. Verfahren zur chemischen Analyse nach Anspruch 10 oder 11, wobei die mikrofluidische Vorrichtung weiterhin eine Fluidauslassende (105) mit einem Fluidauslass (110), wobei sich die innere Wand (25) am Auslassende zum Fluidauslass (110) hin verjüngt.
  13. Verfahren zur chemischen Analyse nach einem der Ansprüche 10 bis 12, wobei in der mikrofluidischen Vorrichtung:
    die Länge der Durchflusskammer (20) etwa 4 cm bis etwa 4,5 cm beträgt und die Breite der Durchflusskammer (20) etwa 1,4 cm bis etwa 1,6 cm beträgt;
    das Volumen der Durchflusskammer (20) etwa 6 µL bis etwa 10 µL beträgt; oder
    die Tiefe der Fluideinlass (10) etwa 20 Mikrometer bis etwa 40 Mikrometer beträgt, die halbkreisförmige Rinne eine Breite von etwa 350 Mikrometer bis etwa 500 Mikrometer besitzt und einen Radius von etwa 3,5 mm bis etwa 4 mm besitzt, die Tiefe der Durchflusskammer (20) etwa 13 Mikrometer bis etwa 20 Mikrometer beträgt, und die Durchflusskammer eine Länge von etwa 3,5 cm bis 4 cm und eine Breite von etwa 1,4 cm bis etwa 1,6 cm aufweist.
  14. Verfahren zur chemischen Analyse nach einem der Ansprüche 10 bis 13, wobei die mikrofluidische Vorrichtung ein Flüssigkeitsaustauschvolumen zwischen etwa 80% und etwa 130% des Volumens der Durchflusskammer besitzt.
  15. Verfahren zur chemischen Analyse nach einem der Ansprüche 10 bis 14, wobei:
    die Flussrate etwa 180 µL/min bis etwa 600 µL/min beträgt und einen Druck von etwa 34,500 Pa bis etwa 206,800 Pa (ungefähr 5 bis ungefähr 30 PSI);
    die Oberfläche (45) des Proben-Chip (35) einen Analysebereich umfasst, die mindestens 0,00015 m2 (1,5 cm2) beträgt;
    der Analysebereich (50) ein Mikroarray ist, der Analysepunkte umfasst;
    der Analysebereich (50) einen Mikroarray aufweist, der Analysepunkte umfasst und wenigstens ein Analysepunkt in Biomolekül umfasst;
    der Analysebereich (50) einen Mikroarray aufweist, der Analysepunkte umfasst und wenigstens einer Analysepunkte umfasst ein Polypeptid oder ein Polynukleotid ist, oder
    der Mikroarray umfasst eine Vielzahl von Polypeptiden, Polynukleotiden oder beides.
EP08798995A 2007-08-29 2008-08-29 Mikrofluidvorrichtung für breitflächige mikroarrays Active EP2240600B1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11/846,883 US20090060786A1 (en) 2007-08-29 2007-08-29 Microfluidic apparatus for wide area microarrays
US11/846,908 US7695976B2 (en) 2007-08-29 2007-08-29 Method for uniform analyte fluid delivery to microarrays
PCT/US2008/074865 WO2009029845A1 (en) 2007-08-29 2008-08-29 Microfluidic apparatus for wide area microarrays

Publications (3)

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EP2240600A1 EP2240600A1 (de) 2010-10-20
EP2240600A4 EP2240600A4 (de) 2011-08-03
EP2240600B1 true EP2240600B1 (de) 2013-03-13

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EP4171818A4 (de) * 2020-06-30 2024-07-10 Plexium, Inc. Fluidische vorrichtung und verfahren
US12508586B2 (en) 2020-06-30 2025-12-30 Plexium, Inc. Uniform fluid flow with constant resistance and velocity

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KR102079307B1 (ko) 2012-02-27 2020-02-19 에꼴 뽈리떼끄닉 뻬데랄 드 로잔느 (으뻬에프엘) 분리 가능한 슬라이드를 갖는 표본 가공 장치
EP3554700B1 (de) 2016-12-19 2024-02-21 Bforcure Thermalisierender mikrofluidischer chip mit variablen temperaturzyklen, system mit solch einem chip und pcr-verfahren zum nachweis von dna-sequenzen

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EP4171818A4 (de) * 2020-06-30 2024-07-10 Plexium, Inc. Fluidische vorrichtung und verfahren
US12508586B2 (en) 2020-06-30 2025-12-30 Plexium, Inc. Uniform fluid flow with constant resistance and velocity

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EP2240600A1 (de) 2010-10-20
EP2240600A4 (de) 2011-08-03

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