EP3505253A1 - Method for extracting nucleic acid and extraction cassette thereof - Google Patents
Method for extracting nucleic acid and extraction cassette thereof Download PDFInfo
- Publication number
- EP3505253A1 EP3505253A1 EP18215536.6A EP18215536A EP3505253A1 EP 3505253 A1 EP3505253 A1 EP 3505253A1 EP 18215536 A EP18215536 A EP 18215536A EP 3505253 A1 EP3505253 A1 EP 3505253A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- compartment
- waste
- liquid
- reaction
- path
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000605 extraction Methods 0.000 title claims abstract description 112
- 102000039446 nucleic acids Human genes 0.000 title claims description 29
- 108020004707 nucleic acids Proteins 0.000 title claims description 29
- 150000007523 nucleic acids Chemical class 0.000 title claims description 29
- 238000000034 method Methods 0.000 title claims description 13
- 239000007788 liquid Substances 0.000 claims abstract description 212
- 239000003599 detergent Substances 0.000 claims description 34
- 239000003480 eluent Substances 0.000 claims description 20
- 239000011358 absorbing material Substances 0.000 claims description 12
- 125000006850 spacer group Chemical group 0.000 claims description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 4
- 230000000284 resting effect Effects 0.000 claims description 4
- 150000003839 salts Chemical class 0.000 description 3
- 238000007886 magnetic bead extraction Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
Images
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/028—Modular arrangements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0668—Trapping microscopic beads
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/141—Preventing contamination, tampering
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/16—Reagents, handling or storing thereof
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0681—Filter
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
Definitions
- the present invention relates to an extraction cassette, and in particular to an extraction cassette with an extraction module.
- the conventional extraction module is only for extracting nucleic acid, which cannot receive waste-liquid. Since the waste-liquid may pollute the filter inside the reaction compartment, the conventional waste-liquid compartment is far away from the collection compartment.
- the waste-liquid compartment and the reaction compartment are disposed on different modules, and the size and cost of the conventional extraction cassette are increased.
- an all-in-one extraction module which extracts nucleic acid by the magnetic bead extraction technology.
- the efficiency and purity of the magnetic bead extraction technology are unqualified.
- an extraction cassette in one embodiment, includes a liquid receiving module and an extraction module.
- the extraction module communicates with the liquid receiving module.
- the extraction module includes an extraction module body, an expansion compartment, a reaction compartment, a filter, a collection compartment, a first waste-liquid compartment and a second waste-liquid compartment.
- the expansion compartment is formed on the extraction module body.
- the reaction compartment is formed on the extraction module body, wherein the reaction compartment includes a reaction compartment inlet, a reaction compartment outlet and a reaction compartment notch, the expansion compartment is connected to the reaction compartment notch, and the reaction compartment notch is located between the reaction compartment inlet and the reaction compartment outlet.
- the filter is disposed in the reaction compartment and corresponding to the reaction compartment outlet.
- the collection compartment is formed on the extraction module body and communicates with the reaction compartment outlet.
- the first waste-liquid compartment is formed on the extraction module body, wherein the first waste-liquid compartment communicates with the reaction compartment outlet.
- the second waste-liquid compartment is formed on the extraction module body, wherein the second waste-liquid compartment communicates with the reaction compartment outlet.
- the reaction compartment includes a cone-shaped portion, the filter is disposed on the cone-shaped portion, and the reaction compartment outlet is formed on one end of the cone-shaped portion.
- the extraction module further includes a first path and a second path, the first path connects the reaction compartment outlet to the first waste-liquid compartment and the second waste-liquid compartment, and the second path connects the reaction compartment outlet to the collection compartment.
- the first path intersects the second path at the reaction compartment outlet, a stopper wall is formed in the second path, and the stopper wall is formed on one end of the second path and is adjacent to the reaction compartment outlet.
- the extraction module further includes a third path and a fourth path, the third path connects the first path to the first waste-liquid compartment, and the fourth path connects the first path to the second waste-liquid compartment.
- the first waste-liquid compartment includes a first waste-liquid compartment connection hole
- the second waste-liquid compartment includes a second waste-liquid compartment connection hole
- the third path connects the first path to the first waste-liquid compartment connection hole
- the fourth path connects the first path to the second waste-liquid compartment connection hole
- at least one portion of the second waste-liquid compartment is located between the first waste-liquid compartment connection hole and the second waste-liquid compartment connection hole.
- At least one portion of the fourth path extends in a first direction, and the first direction is away from the first waste-liquid compartment.
- the second waste-liquid compartment includes a second waste-liquid compartment pressure hole, and at least one portion of the waste-liquid compartment is located between the first waste-liquid compartment and the second waste-liquid compartment pressure hole.
- At least one portion of the second path extends in the first direction.
- the collection compartment includes a collection compartment pressure hole, and at least one portion of the collection compartment is located between the first waste-liquid compartment and the collection compartment pressure hole.
- the extraction module further includes an absorbing material, the absorbing material is disposed in the first waste-liquid compartment, the first waste-liquid compartment includes a first waste-liquid compartment pressure hole, and at least one portion of the absorbing material is located in a space of the first waste-liquid compartment between the first waste-liquid compartment pressure hole and the first waste-liquid compartment connection hole.
- the first waste-liquid compartment pressure hole, the second waste-liquid compartment pressure hole and the collection compartment pressure hole are on the same plane.
- the expansion compartment further includes an expansion compartment inlet, an expansion compartment pressure hole and an expansion compartment spacer, the expansion compartment spacer is located between the expansion compartment inlet and the expansion compartment pressure hole, and the expansion compartment spacer is bent toward the expansion compartment pressure hole.
- a method for extracting nucleic acid includes the following steps. First, an extraction cassette is provided, wherein the extraction cassette includes a liquid receiving module and an extraction module, the extraction module communicates with the liquid receiving module, the extraction module includes a reaction compartment, a filter, a collection compartment, a first waste-liquid compartment and a second waste-liquid compartment. Next, a mixed liquid of sample and alcohol is moved from the liquid receiving module to the extraction module, and the filter captures nucleic acid from the mixed liquid. Then, the mixed liquid is moved to the first waste-liquid compartment. Next, a first detergent is moved from the liquid receiving module to the extraction module, wherein the first detergent passes through the reaction compartment and the filter. Then, the first detergent is moved to the first waste-liquid compartment.
- the method for extracting nucleic acid further includes the following steps. First, a second detergent is moved from the liquid receiving module to the extraction module, wherein the second detergent passes through the reaction compartment and the filter. Then, the second detergent is moved to the second waste-liquid compartment. Next, an eluent is moved from the liquid receiving module to the extraction module, wherein the eluent is resting in the reaction compartment. Then, a positive pressure is provided via a second waste-liquid compartment pressure hole of the second waste-liquid compartment, and a negative pressure is provided via a collection compartment pressure hole of the collection compartment, wherein the eluent with the nucleic acid is moved to the collection compartment.
- the reaction compartment and the waste-liquid compartments are incorporated in one single extraction module, and the size and cost of the extraction cassette are reduced.
- the waste-liquid can be controlled to be moved to the first waste-liquid compartment and the second waste-liquid compartment, and the eluent with the nucleic acid is controlled to be moved to the collection compartment.
- the waste-liquid is prevented from polluting the reaction compartment and the collection compartment.
- Fig. 1A is an assembled view of an extraction cassette C of an embodiment of the present invention.
- Fig. 1B is an exploded view of the extraction cassette C of the embodiment of the present invention.
- the extraction cassette C includes an extraction module 1, a liquid receiving module 2, a sampling module 3 and a connection module 4.
- the extraction cassette C is adapted to be disposed into an analyzer.
- the analyzer includes a first pressure supplying module, an analyzing module and a second pressure supplying module.
- the first pressure supplying module and the second pressure supplying module provide pressure toward the extraction cassette C to control the liquid movement inside the extraction cassette C.
- the analyzing module heats and cools the extraction cassette C, and analyzes the sample inside extraction cassette C.
- the liquid receiving module 2 communicates with the extraction module 1 via the connection module 4.
- Fig. 2 shows the details of the extraction module 1.
- the extraction module 1 includes an extraction module body 19, an expansion compartment 18, a reaction compartment 11, a filter 111, a collection compartment 12, a first waste-liquid compartment 13 and a second waste-liquid compartment 14.
- the expansion compartment 18 is formed on the extraction module body 19.
- the reaction compartment 11 is formed on the extraction module body 19.
- the reaction compartment 11 includes a reaction compartment inlet 114, a reaction compartment outlet 112 and a reaction compartment notch 115.
- the expansion compartment 18 is connected to the reaction compartment notch 115.
- the reaction compartment notch 115 is located between the reaction compartment inlet 114 and the reaction compartment outlet 112.
- the filter 111 is disposed in the reaction compartment 11 and corresponding to the reaction compartment outlet 112.
- the collection compartment 12 is formed on the extraction module body 19 and communicates with the reaction compartment outlet 112.
- the first waste-liquid compartment 13 is formed on the extraction module body 19.
- the first waste-liquid compartment 13 communicates with the reaction compartment outlet 112.
- the second waste-liquid compartment 14 is formed on the extraction module body 19, wherein the second waste-liquid compartment 14 communicates with the reaction compartment outlet 112.
- the expansion compartment 18 further includes an expansion compartment inlet 181, an expansion compartment pressure hole 182 and an expansion compartment spacer 183.
- the expansion compartment spacer 183 is located between the expansion compartment inlet 181 and the expansion compartment pressure hole 182.
- the expansion compartment spacer 183 is bent toward the expansion compartment pressure hole 182.
- the expansion compartment spacer 183 prevents the liquid entering the expansion compartment 18 via the expansion compartment inlet 181 from polluting the expansion compartment pressure hole 182.
- the reaction compartment 11 includes a cone-shaped portion 113.
- the filter 111 is disposed on the cone-shaped portion 113.
- the reaction compartment outlet 112 is formed on one end of the cone-shaped portion 113.
- the extraction module 1 further includes a first path 15 and a second path 16.
- the first path 15 connects the reaction compartment outlet 112 to the first waste-liquid compartment 13 and the second waste-liquid compartment 14.
- the second path 16 connects the reaction compartment outlet 112 to the collection compartment 12.
- the first path 15 intersects the second path 16 at the reaction compartment outlet 112.
- a stopper wall 161 is formed in the second path 16, and the stopper wall 161 is formed on one end of the second path 16 and is adjacent to the reaction compartment outlet 112.
- the extraction module 1 further includes a third path 151 and a fourth path 152.
- the third path 151 connects the first path 15 to the first waste-liquid compartment 13.
- the fourth path 152 connects the first path 15 to the second waste-liquid compartment 14.
- the first waste-liquid compartment 13 includes a first waste-liquid compartment connection hole 131.
- the second waste-liquid compartment 14 includes a second waste-liquid compartment connection hole 141.
- the third path 151 connects the first path 15 to the first waste-liquid compartment connection hole 131.
- the fourth path 152 connects the first path 15 to the second waste-liquid compartment connection hole 141. At least one portion of the second waste-liquid compartment 14 is located between the first waste-liquid compartment connection hole 131 and the second waste-liquid compartment connection hole 141. In one embodiment, at least one portion of the fourth path 152 extends in a first direction Z, and the first direction Z is away from the first waste-liquid compartment 13. The disclosure is not meant to restrict the present invention.
- the second waste-liquid compartment 14 includes a second waste-liquid compartment pressure hole 142. At least one portion of the waste-liquid compartment 14 is located between the first waste-liquid compartment 13 and the second waste-liquid compartment pressure hole 142. In one embodiment, at least one portion of the second path 16 extends in the first direction Z. The disclosure is not meant to restrict the present invention.
- the collection compartment 12 includes a collection compartment pressure hole 121. At least one portion of the collection compartment 12 is located between the first waste-liquid compartment 13 and the collection compartment pressure hole 121.
- the extraction module 1 further includes an absorbing material 17.
- the absorbing material 17 is disposed in the first waste-liquid compartment 13.
- the first waste-liquid compartment 13 includes a first waste-liquid compartment pressure hole 132. At least one portion of the absorbing material 17 is located in a space of the first waste-liquid compartment 13 between the first waste-liquid compartment pressure hole 132 and the first waste-liquid compartment connection hole 131.
- the absorbing material 17 can be sponge.
- the first waste-liquid compartment pressure hole 132, the second waste-liquid compartment pressure hole 142 and the collection compartment pressure hole 121 are on the same plane.
- Figs. 3A ⁇ 3J show the operation of the extraction module 1 of the embodiment of the present invention.
- a mixed liquid 286 of sample and alcohol is moved from the liquid receiving module 2 to the expansion compartment 18 and the reaction compartment 11 via the expansion compartment inlet 181, and the filter 111 captures the nucleic acid from the mixed liquid 286.
- the mixed liquid 286 is moved to the first waste-liquid compartment 13 by a negative pressure ( ⁇ -10kpa) provided via the first waste-liquid compartment pressure hole 132.
- a negative pressure ⁇ -10kpa
- a first detergent 282 is progressively moved from the liquid receiving module 2 to the filter 111 via the expansion compartment inlet 181, expansion compartment 18 and the reaction compartment 11, and the salts concentration and the PH value on the filter 111 is adjusted.
- the first detergent 282 is moved to the first waste-liquid compartment 13 by the negative pressure ( ⁇ -10kpa) provided via the first waste-liquid compartment pressure hole 132.
- a portion of second detergent 283 is moved from the liquid receiving module 2 to the filter 111 via the expansion compartment inlet 181, expansion compartment 18 and the reaction compartment 11, and the salts concentration and the PH value on the filter 111 is adjusted.
- Fig. 3C a first detergent 282 is progressively moved from the liquid receiving module 2 to the filter 111 via the expansion compartment inlet 181, expansion compartment 18 and the reaction compartment 11, and the salts concentration and the PH value on the filter 111 is adjusted.
- the second detergent 283 is moved to the first waste-liquid compartment 13 by the negative pressure ( ⁇ -10kpa) provided via the first waste-liquid compartment pressure hole 132. Then, with reference to Fig. 3G , the other portion of second detergent 283 is moved from the liquid receiving module 2 to the filter 111 via the expansion compartment inlet 181, expansion compartment 18 and the reaction compartment 11, and the salts concentration and the PH value on the filter 111 is adjusted. Next, with reference to Fig. 3H , the second detergent 283 is moved to the second waste-liquid compartment 14 by the negative pressure ( ⁇ -10kpa) provided via the second waste-liquid compartment pressure hole 142. Then, with reference to Fig.
- an eluent 284 is moved from the liquid receiving module 2 to the reaction compartment 11 via the reaction compartment inlet 114, wherein the eluent 284 is resting in the reaction compartment 11 for three minutes, and the nucleic acid is released from the filter 111 to the eluent 284.
- a big positive pressure 45kpa
- a little positive pressure is provided via the second waste-liquid compartment pressure hole 142 to prevent the eluent 284 with the nucleic acid from entering the first path 15.
- the big positive pressure (45kpa) is provided via the liquid receiving module 2 through the reaction compartment inlet 114.
- the first detergent 282 can be progressively moved from the liquid receiving module 2 to the filter 111, or totally moved from the liquid receiving module 2 to the filter 111 in single one movement, so does the second detergent 283.
- the disclosure is not meant to restrict the present invention.
- the first detergent 282 can neutralize PH value
- the second detergent 283 can remove protein and organic impurities.
- the expansion compartment 18 prevents the mixed liquid 286, the first detergent 282 and the second detergent 283 from contacting the reaction compartment inlet 114 to prevent the reaction compartment inlet 114 from being polluted.
- the stopper wall 161 prevents the mixed liquid 286, the first detergent 282 and the second detergent 283 from entering the collection compartment 12.
- the second path 16 has a bent portion 162.
- the bent portion 162 makes the eluent 284 with the nucleic acid to sufficiently enter the collection compartment 12.
- the collection compartment 12 further includes a collection compartment inclined surface 122 and a collection compartment outlet 123.
- the collection compartment inclined surface 122 connects the collection compartment outlet 123.
- the collection compartment inclined surface 122 makes the eluent 284 with the nucleic acid to totally leave the collection compartment 12 via the collection compartment outlet 123 (to the sampling module 3, with reference to Fig. 1B ).
- the bent portion 162 is bent toward the collection compartment inclined surface 122.
- the second detergent 283 finally entering the second waste-liquid compartment 14 is the cleanest waste-liquid.
- the little positive pressure is provided via the second waste-liquid compartment pressure hole 142 to prevent the eluent 284 with the nucleic acid from entering the first path 15. Because the second detergent 283 in the second waste-liquid compartment 14 is the cleanest waste-liquid, the pollution caused by the air inside the second waste-liquid compartment 14 to the eluent 284 can be reduced.
- the waste-liquid in the first waste-liquid compartment 13 contacts the absorbing material 17.
- the absorbing material 17 removes the bubbles of the waste-liquid, and the waste-liquid is prevented from overflowing out of the first waste-liquid compartment pressure hole 132.
- the filter 111 can be silicon filter or other filters.
- the extraction module can be integrally formed.
- a method for extracting nucleic acid includes the following steps. First, an extraction cassette is provided, wherein the extraction cassette includes a liquid receiving module and an extraction module, the extraction module communicates with the liquid receiving module, the extraction module includes a reaction compartment, a filter, a collection compartment, a first waste-liquid compartment and a second waste-liquid compartment (S11). Next, a mixed liquid of sample and alcohol is moved from the liquid receiving module to the extraction module, and the filter captures nucleic acid from the mixed liquid (S12). Then, the mixed liquid is moved to the first waste-liquid compartment (S13).
- a first detergent is moved from the liquid receiving module to the extraction module, wherein the first detergent passes through the reaction compartment and the filter (S14). Then, the first detergent is moved to the first waste-liquid compartment (S15).
- a second detergent is moved from the liquid receiving module to the extraction module, wherein the second detergent passes through the reaction compartment and the filter (S16). Then, the second detergent is moved to the second waste-liquid compartment (S17).
- an eluent is moved from the liquid receiving module to the extraction module, wherein the eluent is resting in the reaction compartment (S18).
- a positive pressure is provided via a second waste-liquid compartment pressure hole of the second waste-liquid compartment, and a negative pressure is provided via a collection compartment pressure hole of the collection compartment, wherein the eluent with the nucleic acid is moved to the collection compartment (S19).
- the reaction compartment and the waste-liquid compartments are incorporated in one single extraction module, and the size and cost of the extraction cassette are reduced.
- the waste-liquid can be controlled to be moved to the first waste-liquid compartment and the second waste-liquid compartment, and the eluent with the nucleic acid is controlled to be moved to the collection compartment.
- the waste-liquid is prevented from polluting the reaction compartment and the collection compartment.
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
- This application claims the benefit of
U.S. Provisional Application No. 62/610444, filed Des. 26, 2017 - This application is a Continuation-In-Part of pending
U.S. patent application Ser. No. 15/018,067, filed Feb. 8, 2016 and entitled "Nucleic acid extracting device ". - This Application claims priority of China Patent Application No.
2018115350186, filed on Dec. 14, 2018 - The present invention relates to an extraction cassette, and in particular to an extraction cassette with an extraction module.
- The conventional extraction module is only for extracting nucleic acid, which cannot receive waste-liquid. Since the waste-liquid may pollute the filter inside the reaction compartment, the conventional waste-liquid compartment is far away from the collection compartment. The waste-liquid compartment and the reaction compartment are disposed on different modules, and the size and cost of the conventional extraction cassette are increased.
- Conventionally, an all-in-one extraction module is provided, which extracts nucleic acid by the magnetic bead extraction technology. However, the efficiency and purity of the magnetic bead extraction technology are unqualified.
- It is an object of the present invention to provide an enhanced extraction cassette, particularly for use in a method for extracting nucleic acids, where the waste-liquid can be prevented from polluting the reaction compartment and the collection compartment using a simple and cost-efficient set-up. It is a further object of the present invention to provide a corresponding enhanced method for extracting nucleic acids.
- These problems are solved by an extraction cassette as claimed by claim 1, and by a method for extracting nucleic acids as claimed by
claim 14. Further advantageous embodiments are the subject-matter of the dependent claims. - In one embodiment, an extraction cassette is provided. The extraction cassette includes a liquid receiving module and an extraction module. The extraction module communicates with the liquid receiving module. The extraction module includes an extraction module body, an expansion compartment, a reaction compartment, a filter, a collection compartment, a first waste-liquid compartment and a second waste-liquid compartment. The expansion compartment is formed on the extraction module body. The reaction compartment is formed on the extraction module body, wherein the reaction compartment includes a reaction compartment inlet, a reaction compartment outlet and a reaction compartment notch, the expansion compartment is connected to the reaction compartment notch, and the reaction compartment notch is located between the reaction compartment inlet and the reaction compartment outlet. The filter is disposed in the reaction compartment and corresponding to the reaction compartment outlet. The collection compartment is formed on the extraction module body and communicates with the reaction compartment outlet. The first waste-liquid compartment is formed on the extraction module body, wherein the first waste-liquid compartment communicates with the reaction compartment outlet. The second waste-liquid compartment is formed on the extraction module body, wherein the second waste-liquid compartment communicates with the reaction compartment outlet.
- In one embodiment, the reaction compartment includes a cone-shaped portion, the filter is disposed on the cone-shaped portion, and the reaction compartment outlet is formed on one end of the cone-shaped portion.
- In one embodiment, the extraction module further includes a first path and a second path, the first path connects the reaction compartment outlet to the first waste-liquid compartment and the second waste-liquid compartment, and the second path connects the reaction compartment outlet to the collection compartment.
- In one embodiment, the first path intersects the second path at the reaction compartment outlet, a stopper wall is formed in the second path, and the stopper wall is formed on one end of the second path and is adjacent to the reaction compartment outlet.
- In one embodiment, the extraction module further includes a third path and a fourth path, the third path connects the first path to the first waste-liquid compartment, and the fourth path connects the first path to the second waste-liquid compartment.
- In one embodiment, the first waste-liquid compartment includes a first waste-liquid compartment connection hole, the second waste-liquid compartment includes a second waste-liquid compartment connection hole, the third path connects the first path to the first waste-liquid compartment connection hole, the fourth path connects the first path to the second waste-liquid compartment connection hole, and at least one portion of the second waste-liquid compartment is located between the first waste-liquid compartment connection hole and the second waste-liquid compartment connection hole.
- In one embodiment, at least one portion of the fourth path extends in a first direction, and the first direction is away from the first waste-liquid compartment.
- In one embodiment, the second waste-liquid compartment includes a second waste-liquid compartment pressure hole, and at least one portion of the waste-liquid compartment is located between the first waste-liquid compartment and the second waste-liquid compartment pressure hole.
- In one embodiment, at least one portion of the second path extends in the first direction.
- In one embodiment, the collection compartment includes a collection compartment pressure hole, and at least one portion of the collection compartment is located between the first waste-liquid compartment and the collection compartment pressure hole.
- In one embodiment, the extraction module further includes an absorbing material, the absorbing material is disposed in the first waste-liquid compartment, the first waste-liquid compartment includes a first waste-liquid compartment pressure hole, and at least one portion of the absorbing material is located in a space of the first waste-liquid compartment between the first waste-liquid compartment pressure hole and the first waste-liquid compartment connection hole.
- In one embodiment, the first waste-liquid compartment pressure hole, the second waste-liquid compartment pressure hole and the collection compartment pressure hole are on the same plane.
- In one embodiment, the expansion compartment further includes an expansion compartment inlet, an expansion compartment pressure hole and an expansion compartment spacer, the expansion compartment spacer is located between the expansion compartment inlet and the expansion compartment pressure hole, and the expansion compartment spacer is bent toward the expansion compartment pressure hole.
- In one embodiment, a method for extracting nucleic acid is provided. The method for extracting nucleic acid includes the following steps. First, an extraction cassette is provided, wherein the extraction cassette includes a liquid receiving module and an extraction module, the extraction module communicates with the liquid receiving module, the extraction module includes a reaction compartment, a filter, a collection compartment, a first waste-liquid compartment and a second waste-liquid compartment. Next, a mixed liquid of sample and alcohol is moved from the liquid receiving module to the extraction module, and the filter captures nucleic acid from the mixed liquid. Then, the mixed liquid is moved to the first waste-liquid compartment. Next, a first detergent is moved from the liquid receiving module to the extraction module, wherein the first detergent passes through the reaction compartment and the filter. Then, the first detergent is moved to the first waste-liquid compartment.
- In one embodiment, the method for extracting nucleic acid further includes the following steps. First, a second detergent is moved from the liquid receiving module to the extraction module, wherein the second detergent passes through the reaction compartment and the filter. Then, the second detergent is moved to the second waste-liquid compartment. Next, an eluent is moved from the liquid receiving module to the extraction module, wherein the eluent is resting in the reaction compartment. Then, a positive pressure is provided via a second waste-liquid compartment pressure hole of the second waste-liquid compartment, and a negative pressure is provided via a collection compartment pressure hole of the collection compartment, wherein the eluent with the nucleic acid is moved to the collection compartment.
- Utilizing the extraction module of the embodiment of the present invention, the reaction compartment and the waste-liquid compartments are incorporated in one single extraction module, and the size and cost of the extraction cassette are reduced. Particularly, by the design of the paths, the reaction compartment, the collection compartment, the first waste-liquid compartment and the second waste-liquid compartment and by the pressure supply, the waste-liquid can be controlled to be moved to the first waste-liquid compartment and the second waste-liquid compartment, and the eluent with the nucleic acid is controlled to be moved to the collection compartment. The waste-liquid is prevented from polluting the reaction compartment and the collection compartment.
- A detailed description is given in the following embodiments with reference to the accompanying drawings.
- The present invention can be more fully understood by reading the subsequent detailed description and examples with references made to the accompanying drawings, wherein:
-
Fig. 1A is an assembled view of an extraction cassette of an embodiment of the present invention; -
Fig. 1B is an exploded view of the extraction cassette of the embodiment of the present invention; -
Fig. 2 shows the details of the extraction module of the embodiment of the present invention; -
Figs. 3A ,3B ,3C ,3D ,3E ,3F ,3G ,3H ,3I and3J show the operation of the extraction module of the embodiment of the present invention; and -
Figs. 4A and4B show a method for extracting nucleic acid of the embodiment of the present invention. - The following description is of the best-contemplated mode of carrying out the present invention. This description is made for the purpose of illustrating the general principles of the present invention and should not be taken in a limiting sense. The scope of the present invention is best determined by reference to the appended claims.
-
Fig. 1A is an assembled view of an extraction cassette C of an embodiment of the present invention.Fig. 1B is an exploded view of the extraction cassette C of the embodiment of the present invention. With reference toFigs. 1A and1B , the extraction cassette C includes an extraction module 1, a liquid receiving module 2, a sampling module 3 and a connection module 4. The extraction cassette C is adapted to be disposed into an analyzer. The analyzer includes a first pressure supplying module, an analyzing module and a second pressure supplying module. The first pressure supplying module and the second pressure supplying module provide pressure toward the extraction cassette C to control the liquid movement inside the extraction cassette C. The analyzing module heats and cools the extraction cassette C, and analyzes the sample inside extraction cassette C. - With reference to
Fig. 1B , the liquid receiving module 2 communicates with the extraction module 1 via the connection module 4.Fig. 2 shows the details of the extraction module 1. With reference toFig. 2 , the extraction module 1 includes anextraction module body 19, anexpansion compartment 18, areaction compartment 11, a filter 111, acollection compartment 12, a first waste-liquid compartment 13 and a second waste-liquid compartment 14. Theexpansion compartment 18 is formed on theextraction module body 19. Thereaction compartment 11 is formed on theextraction module body 19. Thereaction compartment 11 includes areaction compartment inlet 114, areaction compartment outlet 112 and areaction compartment notch 115. Theexpansion compartment 18 is connected to thereaction compartment notch 115. Thereaction compartment notch 115 is located between thereaction compartment inlet 114 and thereaction compartment outlet 112. The filter 111 is disposed in thereaction compartment 11 and corresponding to thereaction compartment outlet 112. Thecollection compartment 12 is formed on theextraction module body 19 and communicates with thereaction compartment outlet 112. The first waste-liquid compartment 13 is formed on theextraction module body 19. The first waste-liquid compartment 13 communicates with thereaction compartment outlet 112. The second waste-liquid compartment 14 is formed on theextraction module body 19, wherein the second waste-liquid compartment 14 communicates with thereaction compartment outlet 112. - With reference to
Fig. 2 , in one embodiment, theexpansion compartment 18 further includes anexpansion compartment inlet 181, an expansioncompartment pressure hole 182 and anexpansion compartment spacer 183. Theexpansion compartment spacer 183 is located between theexpansion compartment inlet 181 and the expansioncompartment pressure hole 182. Theexpansion compartment spacer 183 is bent toward the expansioncompartment pressure hole 182. Theexpansion compartment spacer 183 prevents the liquid entering theexpansion compartment 18 via theexpansion compartment inlet 181 from polluting the expansioncompartment pressure hole 182. - With reference to
Fig. 2 , in one embodiment, thereaction compartment 11 includes a cone-shapedportion 113. The filter 111 is disposed on the cone-shapedportion 113. Thereaction compartment outlet 112 is formed on one end of the cone-shapedportion 113. In one embodiment, the extraction module 1 further includes afirst path 15 and asecond path 16. Thefirst path 15 connects thereaction compartment outlet 112 to the first waste-liquid compartment 13 and the second waste-liquid compartment 14. Thesecond path 16 connects thereaction compartment outlet 112 to thecollection compartment 12. - With reference to
Fig. 2 , in one embodiment, thefirst path 15 intersects thesecond path 16 at thereaction compartment outlet 112. Astopper wall 161 is formed in thesecond path 16, and thestopper wall 161 is formed on one end of thesecond path 16 and is adjacent to thereaction compartment outlet 112. In one embodiment, the extraction module 1 further includes athird path 151 and afourth path 152. Thethird path 151 connects thefirst path 15 to the first waste-liquid compartment 13. Thefourth path 152 connects thefirst path 15 to the second waste-liquid compartment 14. In one embodiment, the first waste-liquid compartment 13 includes a first waste-liquidcompartment connection hole 131. The second waste-liquid compartment 14 includes a second waste-liquidcompartment connection hole 141. Thethird path 151 connects thefirst path 15 to the first waste-liquidcompartment connection hole 131. Thefourth path 152 connects thefirst path 15 to the second waste-liquidcompartment connection hole 141. At least one portion of the second waste-liquid compartment 14 is located between the first waste-liquidcompartment connection hole 131 and the second waste-liquidcompartment connection hole 141. In one embodiment, at least one portion of thefourth path 152 extends in a first direction Z, and the first direction Z is away from the first waste-liquid compartment 13. The disclosure is not meant to restrict the present invention. - With reference to
Fig. 2 , in one embodiment, the second waste-liquid compartment 14 includes a second waste-liquidcompartment pressure hole 142. At least one portion of the waste-liquid compartment 14 is located between the first waste-liquid compartment 13 and the second waste-liquidcompartment pressure hole 142. In one embodiment, at least one portion of thesecond path 16 extends in the first direction Z. The disclosure is not meant to restrict the present invention. In one embodiment, thecollection compartment 12 includes a collectioncompartment pressure hole 121. At least one portion of thecollection compartment 12 is located between the first waste-liquid compartment 13 and the collectioncompartment pressure hole 121. - With reference to
Fig. 2 , in one embodiment, the extraction module 1 further includes an absorbingmaterial 17. The absorbingmaterial 17 is disposed in the first waste-liquid compartment 13. The first waste-liquid compartment 13 includes a first waste-liquidcompartment pressure hole 132. At least one portion of the absorbingmaterial 17 is located in a space of the first waste-liquid compartment 13 between the first waste-liquidcompartment pressure hole 132 and the first waste-liquidcompartment connection hole 131. In one embodiment, the absorbingmaterial 17 can be sponge. In one embodiment, the first waste-liquidcompartment pressure hole 132, the second waste-liquidcompartment pressure hole 142 and the collectioncompartment pressure hole 121 are on the same plane. -
Figs. 3A∼3J show the operation of the extraction module 1 of the embodiment of the present invention. With reference toFig. 3A , amixed liquid 286 of sample and alcohol is moved from the liquid receiving module 2 to theexpansion compartment 18 and thereaction compartment 11 via theexpansion compartment inlet 181, and the filter 111 captures the nucleic acid from themixed liquid 286. Then, with reference toFig. 3B , themixed liquid 286 is moved to the first waste-liquid compartment 13 by a negative pressure (<-10kpa) provided via the first waste-liquidcompartment pressure hole 132. Next, with reference toFig. 3C , afirst detergent 282 is progressively moved from the liquid receiving module 2 to the filter 111 via theexpansion compartment inlet 181,expansion compartment 18 and thereaction compartment 11, and the salts concentration and the PH value on the filter 111 is adjusted. Then, with reference toFig. 3D , thefirst detergent 282 is moved to the first waste-liquid compartment 13 by the negative pressure (<-10kpa) provided via the first waste-liquidcompartment pressure hole 132. Then, with reference toFig. 3E , a portion ofsecond detergent 283 is moved from the liquid receiving module 2 to the filter 111 via theexpansion compartment inlet 181,expansion compartment 18 and thereaction compartment 11, and the salts concentration and the PH value on the filter 111 is adjusted. Next, with reference toFig. 3F , thesecond detergent 283 is moved to the first waste-liquid compartment 13 by the negative pressure (<-10kpa) provided via the first waste-liquidcompartment pressure hole 132. Then, with reference toFig. 3G , the other portion ofsecond detergent 283 is moved from the liquid receiving module 2 to the filter 111 via theexpansion compartment inlet 181,expansion compartment 18 and thereaction compartment 11, and the salts concentration and the PH value on the filter 111 is adjusted. Next, with reference toFig. 3H , thesecond detergent 283 is moved to the second waste-liquid compartment 14 by the negative pressure (<-10kpa) provided via the second waste-liquidcompartment pressure hole 142. Then, with reference toFig. 3I , aneluent 284 is moved from the liquid receiving module 2 to thereaction compartment 11 via thereaction compartment inlet 114, wherein theeluent 284 is resting in thereaction compartment 11 for three minutes, and the nucleic acid is released from the filter 111 to theeluent 284. Next, with reference toFig. 3J , a big positive pressure (45kpa) is provided to move theeluent 284 with the nucleic acid is moved to thecollection compartment 12. With reference toFig. 2 , in the step ofFig. 3J , a little positive pressure is provided via the second waste-liquidcompartment pressure hole 142 to prevent theeluent 284 with the nucleic acid from entering thefirst path 15. In this embodiment, the big positive pressure (45kpa) is provided via the liquid receiving module 2 through thereaction compartment inlet 114. - In the steps of
Figs. 3D and3F , thefirst detergent 282 can be progressively moved from the liquid receiving module 2 to the filter 111, or totally moved from the liquid receiving module 2 to the filter 111 in single one movement, so does thesecond detergent 283. The disclosure is not meant to restrict the present invention. In one embodiment, thefirst detergent 282 can neutralize PH value, and thesecond detergent 283 can remove protein and organic impurities. - In the embodiments above, the
expansion compartment 18 prevents themixed liquid 286, thefirst detergent 282 and thesecond detergent 283 from contacting thereaction compartment inlet 114 to prevent thereaction compartment inlet 114 from being polluted. - With reference to
Fig. 2 , in the embodiment above, thestopper wall 161 prevents themixed liquid 286, thefirst detergent 282 and thesecond detergent 283 from entering thecollection compartment 12. In one embodiment, thesecond path 16 has abent portion 162. Thebent portion 162 makes theeluent 284 with the nucleic acid to sufficiently enter thecollection compartment 12. In one embodiment, thecollection compartment 12 further includes a collection compartment inclinedsurface 122 and acollection compartment outlet 123. The collection compartment inclinedsurface 122 connects thecollection compartment outlet 123. The collection compartment inclinedsurface 122 makes theeluent 284 with the nucleic acid to totally leave thecollection compartment 12 via the collection compartment outlet 123 (to the sampling module 3, with reference toFig. 1B ). In one embodiment, thebent portion 162 is bent toward the collection compartment inclinedsurface 122. - With reference to
Figs. 2 and3H , thesecond detergent 283 finally entering the second waste-liquid compartment 14 is the cleanest waste-liquid. In the step ofFig. 3J , the little positive pressure is provided via the second waste-liquidcompartment pressure hole 142 to prevent theeluent 284 with the nucleic acid from entering thefirst path 15. Because thesecond detergent 283 in the second waste-liquid compartment 14 is the cleanest waste-liquid, the pollution caused by the air inside the second waste-liquid compartment 14 to theeluent 284 can be reduced. - With reference to
Fig. 2 , in the step ofFig. 3F , the waste-liquid in the first waste-liquid compartment 13 contacts the absorbingmaterial 17. The absorbingmaterial 17 removes the bubbles of the waste-liquid, and the waste-liquid is prevented from overflowing out of the first waste-liquidcompartment pressure hole 132. In one embodiment, the filter 111 can be silicon filter or other filters. In one embodiment, the extraction module can be integrally formed. - With reference to
Figs. 4A and4B , in one embodiment, a method for extracting nucleic acid is provided. The method for extracting nucleic acid includes the following steps. First, an extraction cassette is provided, wherein the extraction cassette includes a liquid receiving module and an extraction module, the extraction module communicates with the liquid receiving module, the extraction module includes a reaction compartment, a filter, a collection compartment, a first waste-liquid compartment and a second waste-liquid compartment (S11). Next, a mixed liquid of sample and alcohol is moved from the liquid receiving module to the extraction module, and the filter captures nucleic acid from the mixed liquid (S12). Then, the mixed liquid is moved to the first waste-liquid compartment (S13). Next, a first detergent is moved from the liquid receiving module to the extraction module, wherein the first detergent passes through the reaction compartment and the filter (S14). Then, the first detergent is moved to the first waste-liquid compartment (S15). Next, a second detergent is moved from the liquid receiving module to the extraction module, wherein the second detergent passes through the reaction compartment and the filter (S16). Then, the second detergent is moved to the second waste-liquid compartment (S17). Next, an eluent is moved from the liquid receiving module to the extraction module, wherein the eluent is resting in the reaction compartment (S18). Then, a positive pressure is provided via a second waste-liquid compartment pressure hole of the second waste-liquid compartment, and a negative pressure is provided via a collection compartment pressure hole of the collection compartment, wherein the eluent with the nucleic acid is moved to the collection compartment (S19). - Utilizing the extraction module of the embodiment of the present invention, the reaction compartment and the waste-liquid compartments are incorporated in one single extraction module, and the size and cost of the extraction cassette are reduced. Particularly, by the design of the paths, the reaction compartment, the collection compartment, the first waste-liquid compartment and the second waste-liquid compartment and by the pressure supply, the waste-liquid can be controlled to be moved to the first waste-liquid compartment and the second waste-liquid compartment, and the eluent with the nucleic acid is controlled to be moved to the collection compartment. The waste-liquid is prevented from polluting the reaction compartment and the collection compartment.
- Use of ordinal terms such as "first", "second", "third", etc., in the claims to modify a claim element does not by itself connote any priority, precedence, or order of one claim element over another or the temporal order in which acts of a method are performed, but are used merely as labels to distinguish one claim element having a certain name from another element having the same name (but for use of the ordinal term).
- While the present invention has been described by way of example and in terms of the preferred embodiments, it should be understood that the present invention is not limited to the disclosed embodiments. On the contrary, it is intended to cover various modifications and similar arrangements (as would be apparent to those skilled in the art). Therefore, the scope of the appended claims should be accorded the broadest interpretation so as to encompass all such modifications and similar arrangements.
Claims (15)
- An extraction cassette (C), comprising:a liquid receiving module (2); andan extraction module (1), which communicates with the liquid receiving module, the extraction module comprising:an extraction module body (19);an expansion compartment (18), formed on the extraction module body;a reaction compartment (18), formed on the extraction module body, wherein the reaction compartment comprises a reaction compartment inlet, a reaction compartment outlet and a reaction compartment notch, the expansion compartment is connected to the reaction compartment notch, and the reaction compartment notch is located between the reaction compartment inlet and the reaction compartment outlet;a filter (111), disposed in the reaction compartment and corresponding to the reaction compartment outlet;a collection compartment (12), formed on the extraction module body, the and which communicates with the reaction compartment outlet;a first waste-liquid compartment (13), formed on the extraction module body, wherein the first waste-liquid compartment communicates with the reaction compartment outlet; anda second waste-liquid compartment (14), formed on the extraction module body, wherein the second waste-liquid compartment communicates with the reaction compartment outlet.
- The extraction cassette as claimed in claim 1, wherein the reaction compartment (18) comprises a cone-shaped portion (113), the filter (111) is disposed on the cone-shaped portion (113), and the reaction compartment outlet (112) is formed on one end of the cone-shaped portion (113).
- The extraction cassette as claimed in claim 1 or 2, wherein the extraction module (1) further comprises a first path (15) and a second path (16), the first path connects the reaction compartment outlet (112) to the first waste-liquid compartment (13) and the second waste-liquid compartment (14), and the second path (16) connects the reaction compartment outlet (112) to the collection compartment.
- The extraction cassette as claimed in claim 3, wherein the first path (15) intersects the second path (16) at the reaction compartment outlet (112), a stopper wall (161) is formed in the second path (16), and the stopper wall (161) is formed on one end of the second path (16) and is adjacent to the reaction compartment outlet (112).
- The extraction cassette as claimed in claim 3 or 4, wherein the extraction module (1) further comprises a third path (151) and a fourth path (152), the third path connects the first path (15) to the first waste-liquid compartment (13), and the fourth path (152) connects the first path (15) to the second waste-liquid compartment (14).
- The extraction cassette as claimed in claim 5, wherein the first waste-liquid compartment (13) comprises a first waste-liquid compartment connection hole (13), the second waste-liquid compartment (14) comprises a second waste-liquid compartment connection hole (141), the third path (151) connects the first path (15) to the first waste-liquid compartment connection hole (131), the fourth path (152) connects the first path (15) to the second waste-liquid compartment connection hole (141), and at least one portion of the second waste-liquid compartment (14) is located between the first waste-liquid compartment connection hole (131) and the second waste-liquid compartment connection hole (141).
- The extraction cassette as claimed in claim 6, wherein at least one portion of the fourth path (152) extends in a first direction (Z), and the first direction is away from the first waste-liquid compartment (13).
- The extraction cassette as claimed in claim 6 or 7, wherein the second waste-liquid compartment (14) comprises a second waste-liquid compartment pressure hole (142), and at least one portion of the waste-liquid compartment (14) is located between the first waste-liquid compartment (13) and the second waste-liquid compartment pressure hole (142).
- The extraction cassette as claimed in claim 8, wherein at least one portion of the second path (16) extends in a first direction (Z).
- The extraction cassette as claimed in claim 8 or 9, wherein the collection compartment comprises a collection compartment pressure hole, and at least one portion of the collection compartment is located between the first waste-liquid compartment and the collection compartment pressure hole.
- The extraction cassette as claimed in any of the preceding claims, wherein the extraction module (1) further comprises an absorbing material (17), the absorbing material is disposed in the first waste-liquid compartment (13), the first waste-liquid compartment comprises a first waste-liquid compartment pressure hole (132), and at least one portion of the absorbing material (17) is located in a space of the first waste-liquid compartment between the first waste-liquid compartment pressure hole (132) and the first waste-liquid compartment connection hole (131).
- The extraction cassette as claimed in claim 11, wherein the first waste-liquid compartment pressure hole (132), the second waste-liquid compartment pressure hole (142) and the collection compartment pressure hole (1221) are on the same plane.
- The extraction cassette as claimed in any of the preceding claims, wherein the expansion compartment further comprises an expansion compartment inlet (181), an expansion compartment pressure hole (182) and an expansion compartment spacer (183), the expansion compartment spacer (183) is located between the expansion compartment inlet (181) and the expansion compartment pressure hole (182), and the expansion compartment spacer (183) is bent toward the expansion compartment pressure hole (182).
- A method for extracting nucleic acid, comprising steps of:providing an extraction cassette, wherein the extraction cassette comprises a liquid receiving module and an extraction module, the extraction module communicates with the liquid receiving module, the extraction module comprises a reaction compartment, a filter, a collection compartment, a first waste-liquid compartment and a second waste-liquid compartment;moving a mixed liquid of sample and alcohol from the liquid receiving module to the extraction module, and the filter captures nucleic acid from the mixed liquid;moving the mixed liquid to the first waste-liquid compartment;moving a first detergent from the liquid receiving module to the extraction module, wherein the first detergent passes through the reaction compartment and the filter; andmoving the first detergent to the first waste-liquid compartment.
- The method as claimed in claim 14, further comprising steps of:moving a second detergent from the liquid receiving module to the extraction module, wherein the second detergent passes through the reaction compartment and the filter;moving the second detergent to the second waste-liquid compartment;moving an eluent from the liquid receiving module to the extraction module, wherein the eluent is resting in the reaction compartment; andproviding a positive pressure via a second waste-liquid compartment pressure hole of the second waste-liquid compartment, and providing a negative pressure via a collection compartment pressure hole of the collection compartment, wherein the eluent with the nucleic acid is moved to the collection compartment.
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US201762610444P | 2017-12-26 | 2017-12-26 | |
CN201811535018.6A CN110004141A (en) | 2017-12-26 | 2018-12-14 | Nucleic acid extraction method and its extraction cassette |
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EP3505253B1 EP3505253B1 (en) | 2021-07-28 |
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CN114062094A (en) * | 2020-07-31 | 2022-02-18 | 深圳市帝迈生物技术有限公司 | Hemolysis device, saccharification liquid path system and multifunctional all-in-one machine |
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US20080153078A1 (en) * | 2006-06-15 | 2008-06-26 | Braman Jeffrey C | System for isolating biomolecules from a sample |
GB2516666A (en) * | 2013-07-29 | 2015-02-04 | Atlas Genetics Ltd | Fluidic cartridge for nucleic acid amplification and detection |
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2018
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US20080153078A1 (en) * | 2006-06-15 | 2008-06-26 | Braman Jeffrey C | System for isolating biomolecules from a sample |
GB2516666A (en) * | 2013-07-29 | 2015-02-04 | Atlas Genetics Ltd | Fluidic cartridge for nucleic acid amplification and detection |
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CN114062094A (en) * | 2020-07-31 | 2022-02-18 | 深圳市帝迈生物技术有限公司 | Hemolysis device, saccharification liquid path system and multifunctional all-in-one machine |
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