CN219991545U - DNA draws and integrative device of amplification - Google Patents
DNA draws and integrative device of amplification Download PDFInfo
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- CN219991545U CN219991545U CN202321496618.2U CN202321496618U CN219991545U CN 219991545 U CN219991545 U CN 219991545U CN 202321496618 U CN202321496618 U CN 202321496618U CN 219991545 U CN219991545 U CN 219991545U
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- extraction
- amplification
- dna
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- rotating
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- 230000003321 amplification Effects 0.000 title claims abstract description 49
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 49
- 238000000605 extraction Methods 0.000 claims abstract description 94
- 238000007400 DNA extraction Methods 0.000 claims abstract description 40
- 230000004544 DNA amplification Effects 0.000 claims abstract description 13
- 230000002093 peripheral effect Effects 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 18
- 230000005778 DNA damage Effects 0.000 abstract description 4
- 231100000277 DNA damage Toxicity 0.000 abstract description 4
- 102000039446 nucleic acids Human genes 0.000 abstract description 2
- 108020004707 nucleic acids Proteins 0.000 abstract description 2
- 150000007523 nucleic acids Chemical class 0.000 abstract description 2
- 238000000746 purification Methods 0.000 abstract description 2
- 239000012295 chemical reaction liquid Substances 0.000 description 7
- 238000007789 sealing Methods 0.000 description 5
- 230000000149 penetrating effect Effects 0.000 description 4
- 239000002244 precipitate Substances 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 239000013049 sediment Substances 0.000 description 3
- 239000011324 bead Substances 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 241000206602 Eukaryota Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000000633 nuclear envelope Anatomy 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 238000002205 phenol-chloroform extraction Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the technical field of nucleic acid extraction and purification, in particular to a DNA extraction and amplification integrated device which comprises an operation frame, wherein a connecting plate is arranged on the operation frame and comprises a rotating part and a fixing part, a DNA extraction assembly is arranged on the rotating part, a DNA amplification assembly is arranged on the fixing part, the DNA extraction assembly comprises an extraction cover, a sliding rail is arranged on the side wall of the connecting plate, the arrangement direction of the sliding rail extends from the rotating part to the fixing part, and the side wall of the extraction cover is positioned in the sliding rail. The utility model improves the problem of DNA damage or pollution in the process of DNA extraction.
Description
Technical Field
The utility model relates to the technical field of nucleic acid extraction and purification, in particular to a DNA extraction and amplification integrated device.
Background
At present, DNA is a carrier of genetic information, and for eukaryotes, nuclear genomic DNA is located in the nucleus, and to obtain purified DNA, the structure such as a cell membrane and a nuclear membrane needs to be cracked first to release the DNA, and then impurities such as cell fragments, proteins, lipids and saccharides are removed to purify the DNA; if DNA is required to be extracted from samples such as swabs, feces, soil and the like, impurities such as the swabs, the feces, the soil and the like are also considered to be removed; currently, alternative DNA extraction methods include phenol-chloroform extraction, magnetic bead, silica gel, silica beads, and the like.
The related art can refer to chinese patent with publication number CN217868855U, which discloses a DNA extraction and amplification integrated tube, comprising a tube cover and a tube body, wherein the tube cover is connected with the tube body in a matching way, the inner side of the tube cover is provided with a microneedle, the tube body is provided with a film for storing a reaction mixture, and a cavity is formed below the film and the tube body for amplification reaction.
In the related art, it is necessary to attach DNA to a microneedle and then mount the microneedle to a tube body during DNA amplification, and DNA damage or contamination may still occur during DNA transfer.
Disclosure of Invention
In order to improve the problem of DNA damage or contamination during DNA extraction, the present utility model provides an integrated DNA extraction and amplification device.
The utility model provides a DNA extraction and amplification integrated device which adopts the following technical scheme:
the utility model provides an integrative device of DNA extraction and amplification, includes the operation panel, set up the connecting plate on the operation panel, the connecting plate includes rotation portion and fixed part, be provided with the DNA extraction element on the rotation portion, be provided with the DNA amplification element on the fixed part, the DNA extraction element is including extracting the lid, be provided with the slide rail on the lateral wall of connecting plate, the slide rail sets up the direction and extends to the fixed part by rotation portion, it is located to extract the lateral wall of lid in the slide rail.
Through adopting above-mentioned technical scheme, when carrying out the DNA extraction, will contain the test tube that DNA deposits and connect in extracting and cover, then upset rotation portion, will extract and cover and test tube inversion for DNA adheres to and draws to cover, will extract and cover the turning back, then makes and draws the cover to slide to the DNA amplification subassembly from the slide rail, makes the DNA solution that draws carry out the amplification.
Preferably, the operation frame is provided with an operation rod, the operation rod is connected with the side wall of the rotating part, the operation rod is rotationally connected to the side wall of the operation frame, the side wall of the operation frame is provided with a rotating hole, the operation rod is positioned in the rotating hole, and the operation rod can slide in the rotating hole.
Through adopting above-mentioned technical scheme, the steerable extraction lid of action bars rotates and slides, reduces directly and draws the lid and contact, and the DNA that draws on the extraction lid causes the condition of damage or pollution, makes simultaneously that DNA draws the process of transferring more convenient and fast.
Preferably, the rotating part is rotationally connected with the fixing part along the axial direction, a rotating shaft is arranged on the side wall of the rotating part, which is close to the fixing part, a rotating groove is arranged on the side wall of the fixing part, which is close to the rotating part, and the rotating shaft is positioned in the rotating groove.
Through adopting above-mentioned technical scheme for rotating portion is more stable at pivoted in-process, reduces and draws lid and extraction tube and produce great rocking at pivoted or in-process, further improves the problem that DNA produced damage or pollution at the in-process that shifts.
Preferably, the extracting cover is detachably connected with an extracting pipe, a clamping block is arranged on the peripheral wall of the extracting cover, an L-shaped clamping groove is formed in the pipe orifice of the extracting pipe, and the clamping block is located in the L-shaped clamping groove.
Through adopting above-mentioned technical scheme, when will extracting the pipe and installing to extracting and cover, only need correspond clamping block and L type draw-in groove then rotate and extract the pipe, alright with extracting the pipe and install to extracting and cover, and in extracting the tight connection of cover, be difficult for dropping at the in-process that carries out the pivoted.
Preferably, the rotating part is provided with a clamping table, the pipe orifice of the extraction pipe is provided with a clamping ring along the circumferential direction, and the clamping ring is positioned on the clamping table and is abutted with the outer wall of the clamping table.
Through adopting above-mentioned technical scheme, can further fix the mounted position of extracting pipe, reduce the condition that rocks the dropping at the in-process of experiment extracting pipe, increase the stability of experimental operation.
Preferably, the rotating portion is provided with a guiding inclined surface, and the vertical distance between the guiding inclined surface and the sliding rail is gradually reduced from one end close to the extraction cover to one end far away from the extraction cover.
By adopting the technical scheme, in the process of DNA extraction, the reaction liquid in part of the extraction tube can be adhered to the extraction cover, and in the process of sliding the extraction cover, the reaction liquid adhered to the extraction cover falls onto the guide inclined plane and is guided into the extraction tube by the guide inclined plane.
Preferably, the DNA amplification assembly comprises an amplification tube detachably connected to the fixing part, the operation lever is detachably connected to a side wall of the extraction cover, and the extraction cover is selectively connected to the amplification tube.
Through adopting above-mentioned technical scheme, draw the lid and be connected the back with the amplification pipe, can dismantle the back with the amplification pipe from fixed part, avoided promptly with the contact of DNA sediment, be convenient for other follow-up operations simultaneously.
Preferably, the fixing portion is provided with a mounting hole, the amplification tube is located in the mounting hole, and the side wall of the amplification tube is abutted to the inner wall of the mounting hole.
Through adopting above-mentioned technical scheme, after the setting of extraction lid and amplification pipe, can directly take out amplification pipe and extraction lid from the fixed plate in, shake the amplification pipe and make the DNA sediment that the extraction was covered dissolve in the reactant in the amplification pipe.
In summary, the present utility model includes at least one of the following beneficial technical effects:
1. according to the utility model, the rotating part and the fixing part are arranged, when DNA extraction is carried out, the test tube containing DNA sediment is connected to the extraction cover, then the rotating part is turned over, the extraction cover and the test tube are turned upside down, so that DNA is attached to the extraction cover, the extraction cover is turned back, and then the extraction cover slides from the sliding rail to the DNA amplification assembly, so that the extracted DNA is dissolved for amplification;
2. according to the utility model, the rotating shaft is arranged, so that the rotating part is more stable in the rotating process, the large shaking of the extraction cover and the extraction pipe in the rotating or process is reduced, and the problem of damage or pollution of DNA in the transferring process is further improved;
3. according to the utility model, the guide inclined plane is arranged, so that part of reaction liquid in the extraction tube can be adhered to the extraction cover in the process of DNA extraction, and the reaction liquid adhered to the extraction cover falls onto the guide inclined plane in the process of sliding the extraction cover and is guided into the extraction tube by the guide inclined plane.
Drawings
FIG. 1 is a schematic diagram showing the overall structure of a DNA extraction and amplification integrated apparatus according to an embodiment of the present utility model.
Fig. 2 is a schematic structural view of an operation frame according to an embodiment of the present utility model.
Fig. 3 is a schematic structural view of an extraction cap according to an embodiment of the present utility model.
Reference numerals illustrate: 1. an operation rack; 11. a rotating part; 111. a guide slope; 12. a fixing part; 13. a rotating shaft; 14. a rotating groove; 15. an operation lever; 16. a rotation hole; 17. a slide rail; 171. pushing into the groove; 172. a withdrawal slot; 18. a chute; 19. a clamping table; 191. a mounting hole; 2. a DNA extraction assembly; 21. an extraction tube; 211. an L-shaped clamping groove; 212. a seal ring; 213. a clamping ring; 22. an extraction cap; 221. a sliding part; 222. attaching a needle; 223. a locking groove; 224. sealing grooves; 225. a clamping block; 226. a through groove is arranged; 23. a push rod; 231. a locking block; 3. a DNA amplification assembly; 31. amplification tubes.
Detailed Description
The utility model is described in further detail below with reference to fig. 1-3.
The embodiment of the utility model discloses a DNA extraction and amplification integrated device. Referring to fig. 1, a DNA extraction and amplification integrated apparatus includes a manipulation frame 1, a DNA extraction assembly 2, and a DNA amplification assembly 3.
Referring to fig. 1 and 2, a connection board is provided on an operation rack 1, the connection board is used for connecting a DNA extraction assembly 2 and a DNA amplification assembly 3, the connection board comprises a rotation part 11 and a fixing part 12, a rotation shaft 13 is axially arranged at the end part of the rotation part 11 close to the fixing part 12, a rotation groove 14 is formed at the end part of the fixing part 12, the rotation shaft 13 is positioned in the rotation groove 14, an operation rod 15 is rotatably connected to the operation rack 1, a rotation hole 16 is formed on the side wall of the operation rack 1, the operation rod 15 is positioned in the rotation hole 16, the end part of the operation rod 15 is connected with the rotation part 11, and the operation part can control the rotation part 11 to rotate; the operation frame 1 is provided with a sliding rail 17, the arrangement direction of the sliding rail 17 extends to the fixed part 12 along the rotating part 11, and the end part of the sliding rail 17 is rotationally connected to the side wall of the operation frame 1; the DNA extraction assembly 2 comprises an extraction tube 21 and an extraction cover 22, a sliding part 221 is arranged on the outer peripheral wall of the extraction tube 21, the sliding part 221 is positioned in a sliding rail 17, a pushing groove 171 is formed at the end part of the sliding rail 17, which is close to the extraction tube 21, an withdrawing groove 172 is formed at the end part of the sliding rail 17, which is far away from the extraction tube 21, and the diameters of the pushing groove 171 and the withdrawing groove 172 are the same as the diameter of the sliding part 221; the rotating portion 11 is provided with a guide slope 111, and the vertical distance between the guide slope 111 and the slide rail 17 is gradually reduced from one end close to the extraction cap 22 to one end far from the extraction cap 22, so that a part of the reaction liquid in the extraction tube 21 adheres to the extraction cap 22 during DNA extraction, and the reaction liquid adhering to the extraction cap 22 falls down onto the guide slope 111 during sliding of the extraction cap 22, and is guided into the extraction tube 21 by the guide slope 111.
Referring to fig. 1 and 3, the side wall of the extraction cover 22 is located in the slide rail 17, the end of the extraction cover 22 is provided with an attachment needle 222, the attachment needle 222 is used for attaching the DNA precipitate, the extraction cover 22 is provided with a penetrating groove 226, a push rod 23 is arranged in the penetrating groove 226, the end of the push rod 23 penetrates through the operation frame 1 and extends to the outer side of the operation frame 1, a sliding groove 18 is formed in the side wall of the operation frame 1, which is close to the push rod 23, the push rod 23 is located in the sliding groove 18, a locking block 231 is arranged on the side wall of the push rod 23, which is close to the penetrating groove, the locking block 231 is rotatably connected to the push rod 23, the end of the extraction cover 22 is provided with a locking groove 223, the locking groove 223 is communicated with the penetrating groove, and the locking block 231 is located in the locking groove 223.
After the DNA pellet is attached to the attaching needle 222, the extraction tube 21 is rotated to separate the extraction tube 21 from the extraction cap 22, the slide rail 17 is rotated to the use position, the extraction cap 22 is moved to the push-in groove 171 by sliding the push lever 23 in the slide groove 18, then the push lever 23 is pushed so that the extraction cap 22 slides in the slide rail 17 until sliding to the escape groove 172, and the push lever 23 is operated again to slide in the slide groove 18 so that the extraction cap 22 is withdrawn from the slide rail 17 and moved to be connected to the DNA amplification assembly 3.
Referring to fig. 3, in the present embodiment, an extraction cap 22 is detachably connected to an extraction tube 21, a clamping block 225 is fixedly connected to a side wall of the extraction tube 21, which is close to an attachment needle 222, an L-shaped clamping groove 211 is formed in an inner wall of a tube orifice of the extraction tube 21, when DNA extraction is required, the extraction cap 22 is connected to the extraction tube 21, the clamping block 225 corresponds to an opening of the L-shaped clamping groove 211, and then the extraction tube 21 is pushed to a corner of the clamping block 225, which is abutted against the L-shaped clamping groove 211, and the extraction tube 21 is rotated to fix the extraction tube 21; a circle of sealing groove 224 is formed in the end, close to the extraction cover 21, of the extraction cover 22 in the circumferential direction, a sealing ring 212 is arranged in the end, close to the extraction cover 22, of the extraction cover 21 in the circumferential direction, and the sealing ring 212 is located in the sealing groove 224; the rotating part 11 is provided with a clamping table 19 on the side wall close to the extraction tube 21, the mouth of the extraction tube 21 is provided with a clamping ring 213 along the circumferential direction, and the clamping ring 213 is positioned on the clamping table 19 and is abutted against the outer wall of the clamping table 19 to further fix the extraction tube 21.
Referring to fig. 1, the dna amplification module 3 includes an amplification tube 31, the amplification tube 31 is detachably connected to a fixing portion 12, a mounting hole 191 is formed in the fixing portion 12, the amplification tube is located in the mounting hole 191, a side wall of the amplification tube 31 abuts against an inner wall of the mounting hole 191, and the amplification tube 31 has the same structure as the extraction tube 21.
The embodiment of the utility model provides an implementation principle of an integrated device for DNA extraction and amplification, which comprises the following steps: in the process of DNA extraction and amplification, firstly, an extraction tube 21 containing DNA precipitate is connected with an extraction cover 22, then the extraction rod and the extraction tube 21 are rotated through an operation rod 15, so that DNA precipitate is attached to an attaching needle 222, then the extraction cover 22 and the extraction tube 21 are rotated to separate the extraction cover 22 from the extraction tube 21, the extraction cover 22 is pushed to slide on a slide rail 17 through the operation rod 15, so that the extraction cover 22 is connected with an amplification tube, then the extraction cover 22 and the amplification tube are removed from a fixing part 12 to be mixed, the DNA precipitate on the attaching needle 222 is dissolved in a reaction liquid in the amplification tube, the whole operation process reduces the operation steps of DNA extraction and transfer to the amplification tube, and simultaneously, the situation of false touch on DNA in the DNA transfer is reduced, so that the problem of DNA damage or pollution in the DNA extraction process is solved.
The above embodiments are not intended to limit the scope of the present utility model, so: all equivalent changes in structure, shape and principle of the utility model should be covered in the scope of protection of the utility model.
Claims (8)
1. An integrated DNA extraction and amplification device comprising an operating frame (1), characterized in that: set up the connecting plate on handling frame (1), the connecting plate includes rotation portion (11) and fixed part (12), be provided with DNA extraction element (2) on rotation portion (11), be provided with DNA amplification element (3) on fixed part (12), DNA extraction element (2) are including extracting lid (22), be provided with slide rail (17) on the lateral wall of connecting plate, slide rail (17) set up the direction and extend to fixed part (12) by rotation portion (11), the lateral wall of extracting lid (22) is located in slide rail (17).
2. The integrated DNA extraction and amplification device of claim 1, wherein: be provided with action bars (15) on action bars (1), action bars (15) are connected with the lateral wall of rotating portion (11), action bars (15) rotate and connect on the lateral wall of action bars (1), rotation hole (16) have been seted up on the lateral wall of action bars (1), action bars (15) are located in rotation hole (16).
3. The integrated DNA extraction and amplification device of claim 1, wherein: the rotating part (11) is connected with the fixing part (12) in a rotating way along the axial direction, a rotating shaft (13) is arranged on the side wall, close to the fixing part (12), of the rotating part (11), a rotating groove (14) is formed in the side wall, close to the rotating part (11), of the fixing part (12), and the rotating shaft (13) is located in the rotating groove (14).
4. The integrated DNA extraction and amplification device of claim 1, wherein: the extraction cover (22) is detachably connected with an extraction pipe (21), a clamping block (225) is arranged on the peripheral wall of the extraction cover (22), an L-shaped clamping groove (211) is formed in the pipe orifice of the extraction pipe (21), and the clamping block (225) is located in the L-shaped clamping groove (211).
5. The integrated DNA extraction and amplification device according to claim 4, wherein: the rotary part (11) is provided with a clamping table (19), a pipe orifice of the extraction pipe (21) is circumferentially provided with a clamping ring (213), and the clamping ring (213) is positioned on the clamping table (19) and is abutted with the outer wall of the clamping table (19).
6. The integrated DNA extraction and amplification device of claim 1, wherein: the rotating part (11) is provided with a guide inclined plane, and the vertical distance between one end close to the extraction cover (22) and one end far away from the extraction cover (22) and the sliding rail (17) is gradually reduced.
7. A DNA extraction and amplification integrated device according to claim 2, characterized in that: the DNA amplification assembly (3) comprises an amplification tube (31), the amplification tube (31) is detachably connected to the fixing portion (12), the operating rod (15) is detachably connected to the side wall of the extraction cover (22), and the extraction cover (22) is connected with the amplification tube (31).
8. The integrated DNA extraction and amplification device of claim 7, wherein: the fixing part (12) is provided with a mounting hole (191), the amplification tube (31) is positioned in the mounting hole (191), and the side wall of the amplification tube (31) is abutted with the inner wall of the mounting hole (191).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321496618.2U CN219991545U (en) | 2023-06-12 | 2023-06-12 | DNA draws and integrative device of amplification |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321496618.2U CN219991545U (en) | 2023-06-12 | 2023-06-12 | DNA draws and integrative device of amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219991545U true CN219991545U (en) | 2023-11-10 |
Family
ID=88606885
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321496618.2U Active CN219991545U (en) | 2023-06-12 | 2023-06-12 | DNA draws and integrative device of amplification |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219991545U (en) |
-
2023
- 2023-06-12 CN CN202321496618.2U patent/CN219991545U/en active Active
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