CN220766965U - Magnetic rack for extracting nucleic acid by magnetic bead method - Google Patents
Magnetic rack for extracting nucleic acid by magnetic bead method Download PDFInfo
- Publication number
- CN220766965U CN220766965U CN202322458239.0U CN202322458239U CN220766965U CN 220766965 U CN220766965 U CN 220766965U CN 202322458239 U CN202322458239 U CN 202322458239U CN 220766965 U CN220766965 U CN 220766965U
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- nucleic acid
- magnetic
- bead method
- plate
- magnetic bead
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- 239000011324 bead Substances 0.000 title claims abstract description 53
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 49
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 49
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 49
- 238000000034 method Methods 0.000 title claims abstract description 34
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 14
- 238000009434 installation Methods 0.000 claims abstract description 6
- 229910001172 neodymium magnet Inorganic materials 0.000 claims description 3
- QJVKUMXDEUEQLH-UHFFFAOYSA-N [B].[Fe].[Nd] Chemical compound [B].[Fe].[Nd] QJVKUMXDEUEQLH-UHFFFAOYSA-N 0.000 claims description 2
- 238000000605 extraction Methods 0.000 description 6
- 238000001514 detection method Methods 0.000 description 5
- 238000003780 insertion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a magnetic rack for extracting nucleic acid by a magnetic bead method, which comprises the following steps: a fixed plate and a mounting plate; the fixed plate is arranged in a groove formed by the mounting plate; the fixed plate is provided with a plurality of magnets for adsorbing magnetic beads in the residual reagent of the extracted nucleic acid; elastic clamping pieces for clamping the kit containing the residual reagent of the extracted nucleic acid are arranged at two ends of the fixed plate; the elastic clamping piece is fixed on the lower side of the fixing plate through a screw; mounting concave parts for allowing the elastic clamping pieces to elastically swing are formed at two ends of the mounting plate; the fixing plate and the mounting plate jointly form a step part for clamping into the bottom opening of the kit; the elastic clamping piece is provided with a limiting part which is used for abutting against the bottom edge of the kit to limit the installation position between the magnetic rack for extracting the nucleic acid by the magnetic bead method and the kit. The magnetic rack for extracting nucleic acid by the magnetic bead method can accurately detect whether residual magnetic beads exist or not, can be suitable for kits of different types, and has high universality.
Description
Technical Field
The utility model relates to a magnetic rack for extracting nucleic acid by a magnetic bead method.
Background
The magnetic bead method nucleic acid extraction is a technology of separating magnetic beads from a liquid environment with complex types by using a magnetic field by using functional groups such as hydroxyl groups or carboxyl groups coated on the surfaces of nano magnetic beads to generate chemical bonds with nucleic acid molecules. The magnetic bead method nucleic acid extraction requirement for a large batch of samples can be operated by a magnetic bead method nucleic acid extractor, and the same extraction effect can be achieved by using a magnetic bead method manual nucleic acid extraction magnetic frame for a small batch of samples or users without the nucleic acid extractor.
However, in the manual nucleic acid extraction method using the manual magnetic bead method, there is a possibility that magnetic beads remain in the kit. The magnetic beads are adsorbed with nucleic acid extracts, namely DNA, protein and the like, so that the nucleic acid extraction rate is reduced, and meanwhile, after the detection extracts and the magnetic beads are remained together, certain environmental pollution is caused in a waste recovery link, and corresponding treatment operation is required to be put into aiming at the pollution source, so that the cost of the later treatment of the nucleic acid detection is increased.
Disclosure of Invention
The utility model provides a magnetic rack for extracting nucleic acid by a magnetic bead method, which solves the technical problems, and specifically adopts the following technical scheme:
a magnetic rack for extracting nucleic acid by a magnetic bead method, comprising: a fixed plate and a mounting plate; the fixed plate is arranged in a groove formed by the mounting plate; the fixed plate is provided with a plurality of magnets for adsorbing magnetic beads in the residual reagent of the extracted nucleic acid; elastic clamping pieces for clamping the kit containing the residual reagent of the extracted nucleic acid are arranged at two ends of the fixed plate; the elastic clamping piece is fixed on the lower side of the fixing plate through a screw; mounting concave parts for allowing the elastic clamping pieces to elastically swing are formed at two ends of the mounting plate; the fixing plate and the mounting plate jointly form a step part for clamping into the bottom opening of the kit; the elastic clamping piece is provided with a limiting part which is used for abutting against the bottom edge of the kit to limit the installation position between the magnetic rack for extracting the nucleic acid by the magnetic bead method and the kit.
Further, a guide plate for guiding the bottom edge of the reagent kit to be clamped into the limit part is formed above the limit part.
Further, the side walls on the two sides of the installation concave part are attached to the two side surfaces of the elastic clamping piece.
Further, slots for inserting elastic clamping pieces are formed at the lower sides of the two ends of the fixing plate; the elastic clamping piece is fixed in the slot through the elastic bottom plate; the slot opening of the slot is formed with a caulking groove for allowing the elastic bottom plate to elastically move up and down.
Further, the distance between the elastic clamping piece and the fixed plate is 5mm; the distance between the elastic bottom plate and the upper groove wall of the caulking groove is 5mm.
Further, the fixing plate is formed with a mounting hole; the fixing plate passes through the mounting hole through the screw and is screwed into a screw hole formed by the mounting plate to be fixed in the groove.
Further, the two sides of the mounting plate are formed with holding recesses for a user to hold the fixing plate for disassembling the fixing plate.
Further, the magnet is rotatably mounted in a screw hole formed in the fixing plate by screw threads.
Further, 24 magnets are arranged on the fixed plate; the 24 magnets are distributed in a rectangular shape.
Further, the magnet is made of high-temperature-resistant neodymium iron boron.
The magnetic rack for extracting the nucleic acid by the magnetic bead method has the advantages that the magnetic beads in the residual reagent after extracting the nucleic acid can be quickly adsorbed to the bottom of the kit by the magnet on the fixing plate through magnetic force, so that whether the magnetic beads remain can be judged by whether the magnetic beads are gathered at the bottom of the kit. In addition, through the structural design of elasticity clamping piece, can carry out stable centre gripping to the kit, in unstable detection environment like this, can guarantee that magnet and the bottom of kit keep stable contact, and then can guarantee the validity and the accuracy that remain magnetic bead detected.
Drawings
In order to more clearly illustrate the embodiments of the utility model or the technical solutions of the prior art, the drawings which are used in the description of the embodiments or the prior art will be briefly described, it being obvious that the drawings in the description below are only some embodiments of the utility model, and that other drawings can be obtained according to these drawings without inventive faculty for a person skilled in the art.
FIG. 1 is a schematic diagram of a magnetic rack for extracting nucleic acids by a magnetic bead method according to the present application;
FIG. 2 is a schematic illustration of the underside of a magnetic rack for extracting nucleic acids by the magnetic bead method of FIG. 1;
FIG. 3 is a schematic view of a removal mounting plate of the magnetic rack of FIG. 1 for extracting nucleic acids by the magnetic bead method;
FIG. 4 is a schematic side view of a magnetic rack for extracting nucleic acids according to the magnetic bead method of FIG. 1.
The magnetic rack 10 for extracting nucleic acid by the magnetic bead method, the fixing plate 11, the insertion groove 111, the insertion groove 112, the mounting hole 113, the screw hole 114, the elastic clip 12, the stopper 121, the guide plate 123, the elastic bottom plate 124, the mounting plate 13, the groove 131, the mounting recess 132, the screw hole (not shown), the holding recess 133, the stepped portion 14, the magnet 15, and the screw (not shown).
Detailed Description
Embodiments of the present utility model are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative and intended to explain the present utility model and should not be construed as limiting the utility model.
In the description of the present utility model, unless explicitly stated and limited otherwise, the terms "connected," "connected," and "fixed" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.
In the present utility model, unless expressly stated or limited otherwise, a first feature "above" or "below" a second feature may include both the first and second features being in direct contact, as well as the first and second features not being in direct contact but being in contact with each other through additional features therebetween. Moreover, a first feature being "above," "over" and "on" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature. The first feature being "under", "below" and "beneath" the second feature includes the first feature being directly under and obliquely below the second feature, or simply means that the first feature is less level than the second feature.
As shown in fig. 1 to 4, a magnetic rack 10 for extracting nucleic acid by a magnetic bead method according to the present application comprises: the fixing plate 11 and the mounting plate 13, the mounting plate 13 is formed with a recess 131, and then the fixing plate 11 is mounted in the recess 131. The fixing plate 11 is provided with a plurality of magnets 15, and the magnets 15 are used for adsorbing magnetic beads in the residual reagent of the extracted nucleic acid. In this scheme, both ends of the fixing plate 11 are provided with elastic clips 12 for clamping the kit containing the residual reagents of the extracted nucleic acid, thereby ensuring the stability of the fit between the kit magnets 15. The elastic clip 12 is fixed to the underside of the fixing plate 11 by screws. Mounting recesses 132 are formed at both ends of the mounting plate 13, and the mounting recesses 132 allow the elastic clips 12 to elastically swing, that is, improve the versatility of the magnetic rack detection when different cartridges can be applied by the elastic swinging of the elastic clips 12. The fixing plate 11 and the mounting plate 13 together form a step portion 14, and the step portion 14 is just caught in an opening at the bottom of the reagent cartridge when the reagent cartridge is in contact with the magnet 15, so that the reagent cartridge can be prevented from being positionally shifted. The elastic clamping piece 12 is formed with a limiting part 121, and the limiting part 121 is used for abutting against the bottom edge of the kit, so that the installation position between the magnetic rack 10 for extracting nucleic acid by the magnetic bead method and the kit is limited, namely, the bottom edge of the kit is clamped below the limiting part, and the contact stability of the kit and the magnetic rack is further ensured.
The magnetic rack 10 for extracting nucleic acid by the magnetic bead method can quickly adsorb the magnetic beads in the residual reagent after extracting nucleic acid to the bottom of the kit by the magnetic force through the magnet 15 on the fixing plate 11, so that whether the magnetic beads remain can be judged by whether the magnetic beads are accumulated at the bottom of the kit. In addition, through the structural design of elastic clamping piece 12, can carry out stable centre gripping to the kit, in unstable detection environment like this, can guarantee that magnet 15 and the bottom of kit keep stable contact, and then can guarantee the validity and the accuracy that remain the magnetic bead and detect.
Further, a guide plate 123 is formed above the stopper 121. The guide plate 123 is used to guide the bottom edge of the kit to be caught in the stopper 121 when detecting whether there are any remaining magnetic beads in the kit.
As a further solution, the side walls on both sides of the mounting recess 132 are attached to both side surfaces of the elastic clip 12, so that the elastic clip 12 can be limited to perform elastic movement along the left-right direction, so that deformation is not easy to occur, stability of the structure is ensured, and service life of the elastic clip 12 is prolonged.
As a further alternative, both ends of the fixing plate 11 are formed with insertion grooves 111 at lower sides for inserting the elastic clips 12. The resilient clip 12 forms a resilient base 124, and then the resilient clip 12 is secured into the slot 111 by the resilient base 124. A caulking groove 112 is also formed in the notch of the slot 111, and the caulking groove 112 is used for allowing the elastic bottom plate 124 to elastically move up and down, so that kits having bottom edges of different thicknesses can be adapted, and versatility is higher.
Specifically, the distance between the elastic clamping piece 12 and the fixing plate 11 is 5mm, and the distance between the elastic bottom plate 124 and the upper groove wall of the caulking groove 112 is 5mm. Such size limitation can be applied to various kits on the market, while the clamping stability is also relatively high.
Further, the fixing plate 11 is formed with the mounting hole 113, and the fixing plate 11 is fixed in the groove 131 by passing through the mounting hole 113 through a screw and screwing into a screw hole formed in the mounting plate 13, so that the assembly and the disassembly are convenient.
Further, the holding concave portions 133 are formed on both sides of the mounting plate 13, and the user can hold the fixing plate 11 by hand through the holding concave portions 133 to detach the fixing plate 11, thereby improving the assembly efficiency.
As a further solution, the fixing plate 11 is formed with a threaded hole 114, and when the magnet 15 is mounted in the threaded hole 114 by screwing, not only the convenience of mounting and dismounting the magnet 15 can be improved, but also kits having different sizes can be adapted, for example, the hole positions of deep hole plates of some kits are relatively high, and the magnet 15 needs to be rotated upwards to improve the extending height of the magnet 15.
As a further solution, 24 magnets 15 are provided on the fixing plate 11, and the 24 magnets 15 are distributed in a rectangular shape to fit the bottom of the kit. The magnet 15 is made of N52-grade high-temperature-resistant neodymium iron boron magnet 15, and has high magnetic flux and good adsorption effect, and 24 magnets 15 are used for adsorbing 96 Kong Cizhu, so that the efficiency is high and the cost is low.
The foregoing has shown and described the basic principles, principal features and advantages of the utility model. It will be appreciated by persons skilled in the art that the above embodiments are not intended to limit the utility model in any way, and that all technical solutions obtained by means of equivalent substitutions or equivalent transformations fall within the scope of the utility model.
Claims (10)
1. A magnetic rack for extracting nucleic acid by a magnetic bead method, comprising: a fixed plate and a mounting plate;
the fixing plate is arranged in a groove formed by the mounting plate;
the fixed plate is provided with a plurality of magnets for adsorbing magnetic beads in the residual reagent of the extracted nucleic acid;
elastic clamping pieces for clamping a kit containing the residual reagent of the extracted nucleic acid are arranged at two ends of the fixing plate;
the elastic clamping piece is fixed on the lower side of the fixing plate through a screw;
mounting concave parts for allowing the elastic clamping pieces to elastically swing are formed at two ends of the mounting plate;
the fixing plate and the mounting plate jointly form a step part for being clamped into an opening at the bottom of the kit;
the elastic clamping piece is provided with a limiting part which is used for abutting against the bottom edge of the kit to limit the installation position between the magnetic rack for extracting the nucleic acid by the magnetic bead method and the kit.
2. The magnetic rack for extracting nucleic acid by a magnetic bead method according to claim 1, wherein,
the top of spacing portion is formed with the guide board that is used for guiding the bottom edge card of kit to go into in the spacing portion.
3. The magnetic rack for extracting nucleic acid by a magnetic bead method according to claim 1, wherein,
the side walls at the two sides of the installation concave part are attached to the two side surfaces of the elastic clamping piece.
4. The magnetic rack for extracting nucleic acid by a magnetic bead method according to claim 1, wherein,
the two ends of the fixing plate are provided with slots for inserting the elastic clamping pieces at the lower sides;
the elastic clamping piece is fixed into the slot through the formed elastic bottom plate;
the slot opening of the slot is provided with a caulking groove for allowing the elastic bottom plate to elastically move up and down.
5. The magnetic rack for extracting nucleic acid by a magnetic bead method according to claim 4, wherein,
the distance between the elastic clamping piece and the fixed plate is 5mm;
the distance between the elastic bottom plate and the upper groove wall of the caulking groove is 5mm.
6. The magnetic rack for extracting nucleic acid by a magnetic bead method according to claim 1, wherein,
the fixing plate is provided with a mounting hole;
the fixing plate passes through the mounting hole through a screw and is screwed into a screw hole formed by the mounting plate to be fixed in the groove.
7. The magnetic rack for extracting nucleic acid by a magnetic bead method according to claim 1, wherein,
two sides of the mounting plate are provided with holding concave parts for users to hold the fixing plate to detach the fixing plate.
8. The magnetic rack for extracting nucleic acid by a magnetic bead method according to claim 1, wherein,
the magnet is rotatably arranged in a threaded hole formed in the fixing plate through threads.
9. The magnetic rack for extracting nucleic acid by a magnetic bead method according to claim 1, wherein,
24 magnets are arranged on the fixed plate;
the 24 magnets are distributed in a rectangular shape.
10. The magnetic rack for extracting nucleic acid by a magnetic bead method according to claim 9, wherein,
the magnet is made of high-temperature-resistant neodymium iron boron.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322458239.0U CN220766965U (en) | 2023-09-11 | 2023-09-11 | Magnetic rack for extracting nucleic acid by magnetic bead method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322458239.0U CN220766965U (en) | 2023-09-11 | 2023-09-11 | Magnetic rack for extracting nucleic acid by magnetic bead method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220766965U true CN220766965U (en) | 2024-04-12 |
Family
ID=90598613
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322458239.0U Active CN220766965U (en) | 2023-09-11 | 2023-09-11 | Magnetic rack for extracting nucleic acid by magnetic bead method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220766965U (en) |
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2023
- 2023-09-11 CN CN202322458239.0U patent/CN220766965U/en active Active
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